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The map label for this gene is yneF [H]

Identifier: 159185045

GI number: 159185045

Start: 2054055

End: 2054864

Strand: Reverse

Name: yneF [H]

Synonym: Atu2091

Alternate gene names: 159185045

Gene position: 2054864-2054055 (Counterclockwise)

Preceding gene: 15889376

Following gene: 159185044

Centisome position: 72.31

GC content: 58.52

Gene sequence:

>810_bases
ATGATGTTGGGTCTTTGGAAAAAACCGATCGAGATGATGAGTTTTTCGGTCGCTACGCAGGCGGGTCGCCGGCGGCTGGG
GCCTCTTGAATTCTCCGTTCGTATCGCTTGTCTCGTGACGCTCATTGTCATCGTGATGTGCACGGGTGGCCTGACGCTTC
TCGCGCAGTTCGGGCTCGTGACCGATCTCTTCAACGGGATCATGTTAAGCGTGACGCTCGGCGGGGCTGTCGCCTTTACG
GTGACGTTGCTGGTCTGCTGGCTGAATGCGAGGGAAGTGCAAATTCTCGTGCAGTCCCACGAACGCTTCCTGCACCTGAG
CCATACGGACGCGCTGACTGGCCTTAGCAACCGGCTAGGCCTTTATGCGGCATGCGCCGAACTTGGCAGCGACTATTGTG
TTGCCTTTTTGGACATCGATCATTTCAAAGCGGTGAACGACGGGTACAGCCATCTTGTTGGTGACCTCGTCATCGCAAGC
GTCGCCCGCCGGATAAAGGAGCATTTCGATCCTCCCGCGCATGTCGCCCGCCTGGGTGGCGAGGAGTTCGTCGTCGTGCA
GGAAACCAGCCCGCTCGCGTTTCTGCAGATGTGCGAAAGGGTTCGTGCGGCGATCGAGACGACGCCGGTGGCGTATCAGG
ATCTGCGAATTACGGTGACGATTTCAATTGGCGTCGCCTTTCGCGGCGACGCCGAAATCTTCGACAAGGTCATGCACAAT
GCCGATCTCGCGCTTTATCGGGCCAAGGGCGGAGGACGCAATCGCGTTTGCATGAGCGGTCATGTCGAGCGGCGCCAGGC
AGTGGCTTAA

Upstream 100 bases:

>100_bases
CAAATACGTGAGTGGGCGAAGTAACGCCCCGCTCCTTAAATATTAAATCTTTCTTTATTTGCAAATACCAACGGCATTAC
GTGATTTAGGCACTCTGAGC

Downstream 100 bases:

>100_bases
GCAGAAATAGACAAGGAAAAGCCCGCGAAACGAAGGTTTCGCGGGCTTTTTGACGTCGATGGTCTCAGATCAGACTTCGT
CCTTGCGGGACAGAAAAATG

Product: GGDEF family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MMLGLWKKPIEMMSFSVATQAGRRRLGPLEFSVRIACLVTLIVIVMCTGGLTLLAQFGLVTDLFNGIMLSVTLGGAVAFT
VTLLVCWLNAREVQILVQSHERFLHLSHTDALTGLSNRLGLYAACAELGSDYCVAFLDIDHFKAVNDGYSHLVGDLVIAS
VARRIKEHFDPPAHVARLGGEEFVVVQETSPLAFLQMCERVRAAIETTPVAYQDLRITVTISIGVAFRGDAEIFDKVMHN
ADLALYRAKGGGRNRVCMSGHVERRQAVA

Sequences:

>Translated_269_residues
MMLGLWKKPIEMMSFSVATQAGRRRLGPLEFSVRIACLVTLIVIVMCTGGLTLLAQFGLVTDLFNGIMLSVTLGGAVAFT
VTLLVCWLNAREVQILVQSHERFLHLSHTDALTGLSNRLGLYAACAELGSDYCVAFLDIDHFKAVNDGYSHLVGDLVIAS
VARRIKEHFDPPAHVARLGGEEFVVVQETSPLAFLQMCERVRAAIETTPVAYQDLRITVTISIGVAFRGDAEIFDKVMHN
ADLALYRAKGGGRNRVCMSGHVERRQAVA
>Mature_269_residues
MMLGLWKKPIEMMSFSVATQAGRRRLGPLEFSVRIACLVTLIVIVMCTGGLTLLAQFGLVTDLFNGIMLSVTLGGAVAFT
VTLLVCWLNAREVQILVQSHERFLHLSHTDALTGLSNRLGLYAACAELGSDYCVAFLDIDHFKAVNDGYSHLVGDLVIAS
VARRIKEHFDPPAHVARLGGEEFVVVQETSPLAFLQMCERVRAAIETTPVAYQDLRITVTISIGVAFRGDAEIFDKVMHN
ADLALYRAKGGGRNRVCMSGHVERRQAVA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787802, Length=243, Percent_Identity=32.0987654320988, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI87081881, Length=164, Percent_Identity=35.9756097560976, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787262, Length=248, Percent_Identity=32.6612903225806, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786584, Length=243, Percent_Identity=29.6296296296296, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI145693134, Length=159, Percent_Identity=34.5911949685535, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1787541, Length=168, Percent_Identity=31.547619047619, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI87082007, Length=213, Percent_Identity=31.4553990610329, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788085, Length=158, Percent_Identity=34.8101265822785, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787816, Length=163, Percent_Identity=34.3558282208589, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081977, Length=169, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1788956, Length=170, Percent_Identity=32.3529411764706, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788381, Length=189, Percent_Identity=28.5714285714286, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 29433; Mature: 29433

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLGLWKKPIEMMSFSVATQAGRRRLGPLEFSVRIACLVTLIVIVMCTGGLTLLAQFGLV
CCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TDLFNGIMLSVTLGGAVAFTVTLLVCWLNAREVQILVQSHERFLHLSHTDALTGLSNRLG
HHHHCCCEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEECCHHHHHHHHHHHH
LYAACAELGSDYCVAFLDIDHFKAVNDGYSHLVGDLVIASVARRIKEHFDPPAHVARLGG
HHHHHHHHCCCEEEEEEEHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
EEFVVVQETSPLAFLQMCERVRAAIETTPVAYQDLRITVTISIGVAFRGDAEIFDKVMHN
CEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEEEEECCCHHHHHHHHCC
ADLALYRAKGGGRNRVCMSGHVERRQAVA
CCEEEEEECCCCCCCEEECCCHHHHHCCC
>Mature Secondary Structure
MMLGLWKKPIEMMSFSVATQAGRRRLGPLEFSVRIACLVTLIVIVMCTGGLTLLAQFGLV
CCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TDLFNGIMLSVTLGGAVAFTVTLLVCWLNAREVQILVQSHERFLHLSHTDALTGLSNRLG
HHHHCCCEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEECCHHHHHHHHHHHH
LYAACAELGSDYCVAFLDIDHFKAVNDGYSHLVGDLVIASVARRIKEHFDPPAHVARLGG
HHHHHHHHCCCEEEEEEEHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
EEFVVVQETSPLAFLQMCERVRAAIETTPVAYQDLRITVTISIGVAFRGDAEIFDKVMHN
CEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEEEEECCCHHHHHHHHCC
ADLALYRAKGGGRNRVCMSGHVERRQAVA
CCEEEEEECCCCCCCEEECCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]