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The map label for this gene is pcrA [H]

Identifier: 159185029

GI number: 159185029

Start: 2019745

End: 2022219

Strand: Direct

Name: pcrA [H]

Synonym: Atu2057

Alternate gene names: 159185029

Gene position: 2019745-2022219 (Clockwise)

Preceding gene: 15889342

Following gene: 159185030

Centisome position: 71.08

GC content: 60.69

Gene sequence:

>2475_bases
ATGAGCAACAGTTTCGACGATATGCCGTTCTTCGATGAAGAACCCGTGGCACCGCGCAGGGCGACCAACAATGCCCCTTC
GGAAAACGGCGGCGGGCTTGGCATTGCCGCCCGCGCCATGGCGGCGCGCGACCAGGTGCGGCCGGCACACGATTATCTTT
CCGGCCTCAACCCGGAACAGCGCGAGGCCGTGGAAACGCTTGACGGACCGGTTCTCGTTCTCGCCGGCGCCGGCACCGGC
AAGACCCGCGTTCTGACGACCCGCATCGCCCATATTCTCGCCACCGGCCGCGCCTATCCAAGCCAGATCCTTGCCGTGAC
CTTCACCAACAAGGCGGCCCGCGAGATGAAGGAACGTATCGGCGTCCTCGTCGGCGGCGCTGTCGAGGGCATGCCGTGGC
TTGGCACCTTCCACTCCATCGGCGTCAAGCTTCTGCGCCGCCATGCCGAGCTCATCGGCCTGAAATCGGATTTCACCATT
CTCGACACGGATGATGTGGTGCGGCTGATCAAGCAGCTCATTCAGGCCGAAGGTCTTGATGACAAGCGCTGGCCGGCAAA
ACAGTTCGCCGGCATGATCGACGGCTGGAAGAACAAGGGGCTGACGCCACCGGATATTCCCGAGGGTGACAGCCGCGCCT
TTGCCAACGGCAAGGGCCGCGAACTTTACGCCGCCTATCAGGCCCGGCTGAAAACGTTGAACGCCTGCGATTTCGGTGAT
CTCTTGCTGCACCCGATCAGCATCTTCCGCCGCCATACGGATATTCTGAAAGAGTATCACCAGAAGTTCCGTTATATTCT
GGTGGACGAATATCAGGACACCAACACGGCGCAATATATGTGGCTGCGGCTGCTTGCCCAGCGCGCCAAGGACGAGCCGC
AGAATGTCTGCTGCGTCGGTGACGACGACCAGTCGATCTATGGCTGGCGCGGCGCGGAAGTGGACAATATCCTGCGCTTC
GACAAGGATTTTCCCGGTGCCAAGGTCATCAAGCTTGAGCGTAACTACCGTTCCACCGAACATATTCTCGGCGCTGCCGG
CCATCTGATCGCCCACAACGAGGGCCGTCTCGGCAAGACGTTGTTTACCGAGCGCAGCAGTCCGGACGATGAAAAGGTCG
TGGTGCACGCCGCCTGGGATTCGGAAGAAGAGGCCCGCGCGGTCGGAGAGGAAATCGAGCAACTCCAGCGTAAGAACTAC
AAGCTGAACGACATGGCCATCCTCGTTCGCGCTTCCTTCCAGATGCGCGAATTCGAAGACCGCTTCGTGACGCTTGGCCT
CAATTACCGCGTCATCGGCGGCCCGCGCTTTTATGAGCGCCTCGAAATCCGCGATGCCATGGCCTATTTCCGGCTGGTCT
GCCAACCGGCCGACGATCTCGCCTTCGAGCGGATCGTCAATACGCCAAAGCGTGGCCTTGGCGATACGACGATCCGTAAC
CTGCACGACTATGCCCGCGCCCGCGATATCCCGATGCTGGCAGCCGCCGCCGACATCATCGAGACCGACGAGTTGAAGCC
GAAAGCGCGCAAGGCGCTGTTCGATGTGGTGCAGGATTTCCGCCGCTGGCAGGGCCTGCTGGAAAACACCGAACACACCA
CGCTTGCCGAACAGATTCTCGACGAAAGCGGCTATACCGCCATGTGGCAGGCCGACAAGACGGCCGAAGCACCGGGACGG
CTTGAGAACCTGAAGGAACTCATCCGCTCGATGGAATCCTTCGAAAGCATGCGTGGATTTCTGGAACACGTCGCCCTGGT
TATGGATGCGGAACAGAATGCCGAGCTGGATGCCGTCTCCATCATGACGCTGCATTCCGCCAAGGGTCTGGAATTCGACA
CCGTCTTCCTGCCCGGTTGGGAAGAAGGCCTGTTTCCCCACCAGCGGGCGCTGGACGAAGGCGGTCGCTCCGGTCTGGAG
GAAGAACGTCGTCTGGCTTATGTCGGCATCACCCGCGCCAAGAAGCTCTGCCATATCTGGTTCGTCTCGAACCGGCGCAT
TCACGGGCTGTGGCAATCCACCCTGCCCTCGCGTTTCCTCGACGAACTGCCGCCCGCCCATGTGGATGTCGCGGCTTCCG
ACAGCAATTATGGCGGTTATGGCGGACGCGGCGGTTACGGCCAGTCCCGTTTCGACAAGGCCGATCCCTTCACCAACAAT
TACTCCACCCCCGGCTGGAAACGCGCCCAGCAGAACCGCAGCGACGCCACCCGCGACAATTGGGGCACACGCTCCGGCCA
TGCGGTGGAGCGCATCGGTTACGGCGAAAGCGGCCCGCGCACCCGCACCATCGACGGCGAACTGGTGGCGAAATCCGTGG
CTGACAAGCCCTCGAAATTCTTCGTCGGCGACCGTGTTTTCCACCTCAAATTCGGCAATGGCAATATTTCCGCGATCGAA
GGCAATAAATTGACCATCAACTTCGACCGTGCCGGCGAAAAGCGCGTGCTGGATGGATTTGTGGAAAAGGTTTGA

Upstream 100 bases:

>100_bases
CCGAAAAAATGCCTTGGAATAGGCGTTTTGACTGACGATACCGGAATAAAAGCAGAACACGGTTCTGGCCTTTATATCCC
GAATCACTACATTGGGCCGC

Downstream 100 bases:

>100_bases
TCCGACAACCATGAGTGCCTGGAGAGCGCTTTTCCCATAGCCGATGTCACGCCATTGCAATAAAGGAGTTGCAAAGGCGA
ACTGAAAACCGGGACGGGAC

Product: DNA helicase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 824; Mature: 823

Protein sequence:

>824_residues
MSNSFDDMPFFDEEPVAPRRATNNAPSENGGGLGIAARAMAARDQVRPAHDYLSGLNPEQREAVETLDGPVLVLAGAGTG
KTRVLTTRIAHILATGRAYPSQILAVTFTNKAAREMKERIGVLVGGAVEGMPWLGTFHSIGVKLLRRHAELIGLKSDFTI
LDTDDVVRLIKQLIQAEGLDDKRWPAKQFAGMIDGWKNKGLTPPDIPEGDSRAFANGKGRELYAAYQARLKTLNACDFGD
LLLHPISIFRRHTDILKEYHQKFRYILVDEYQDTNTAQYMWLRLLAQRAKDEPQNVCCVGDDDQSIYGWRGAEVDNILRF
DKDFPGAKVIKLERNYRSTEHILGAAGHLIAHNEGRLGKTLFTERSSPDDEKVVVHAAWDSEEEARAVGEEIEQLQRKNY
KLNDMAILVRASFQMREFEDRFVTLGLNYRVIGGPRFYERLEIRDAMAYFRLVCQPADDLAFERIVNTPKRGLGDTTIRN
LHDYARARDIPMLAAAADIIETDELKPKARKALFDVVQDFRRWQGLLENTEHTTLAEQILDESGYTAMWQADKTAEAPGR
LENLKELIRSMESFESMRGFLEHVALVMDAEQNAELDAVSIMTLHSAKGLEFDTVFLPGWEEGLFPHQRALDEGGRSGLE
EERRLAYVGITRAKKLCHIWFVSNRRIHGLWQSTLPSRFLDELPPAHVDVAASDSNYGGYGGRGGYGQSRFDKADPFTNN
YSTPGWKRAQQNRSDATRDNWGTRSGHAVERIGYGESGPRTRTIDGELVAKSVADKPSKFFVGDRVFHLKFGNGNISAIE
GNKLTINFDRAGEKRVLDGFVEKV

Sequences:

>Translated_824_residues
MSNSFDDMPFFDEEPVAPRRATNNAPSENGGGLGIAARAMAARDQVRPAHDYLSGLNPEQREAVETLDGPVLVLAGAGTG
KTRVLTTRIAHILATGRAYPSQILAVTFTNKAAREMKERIGVLVGGAVEGMPWLGTFHSIGVKLLRRHAELIGLKSDFTI
LDTDDVVRLIKQLIQAEGLDDKRWPAKQFAGMIDGWKNKGLTPPDIPEGDSRAFANGKGRELYAAYQARLKTLNACDFGD
LLLHPISIFRRHTDILKEYHQKFRYILVDEYQDTNTAQYMWLRLLAQRAKDEPQNVCCVGDDDQSIYGWRGAEVDNILRF
DKDFPGAKVIKLERNYRSTEHILGAAGHLIAHNEGRLGKTLFTERSSPDDEKVVVHAAWDSEEEARAVGEEIEQLQRKNY
KLNDMAILVRASFQMREFEDRFVTLGLNYRVIGGPRFYERLEIRDAMAYFRLVCQPADDLAFERIVNTPKRGLGDTTIRN
LHDYARARDIPMLAAAADIIETDELKPKARKALFDVVQDFRRWQGLLENTEHTTLAEQILDESGYTAMWQADKTAEAPGR
LENLKELIRSMESFESMRGFLEHVALVMDAEQNAELDAVSIMTLHSAKGLEFDTVFLPGWEEGLFPHQRALDEGGRSGLE
EERRLAYVGITRAKKLCHIWFVSNRRIHGLWQSTLPSRFLDELPPAHVDVAASDSNYGGYGGRGGYGQSRFDKADPFTNN
YSTPGWKRAQQNRSDATRDNWGTRSGHAVERIGYGESGPRTRTIDGELVAKSVADKPSKFFVGDRVFHLKFGNGNISAIE
GNKLTINFDRAGEKRVLDGFVEKV
>Mature_823_residues
SNSFDDMPFFDEEPVAPRRATNNAPSENGGGLGIAARAMAARDQVRPAHDYLSGLNPEQREAVETLDGPVLVLAGAGTGK
TRVLTTRIAHILATGRAYPSQILAVTFTNKAAREMKERIGVLVGGAVEGMPWLGTFHSIGVKLLRRHAELIGLKSDFTIL
DTDDVVRLIKQLIQAEGLDDKRWPAKQFAGMIDGWKNKGLTPPDIPEGDSRAFANGKGRELYAAYQARLKTLNACDFGDL
LLHPISIFRRHTDILKEYHQKFRYILVDEYQDTNTAQYMWLRLLAQRAKDEPQNVCCVGDDDQSIYGWRGAEVDNILRFD
KDFPGAKVIKLERNYRSTEHILGAAGHLIAHNEGRLGKTLFTERSSPDDEKVVVHAAWDSEEEARAVGEEIEQLQRKNYK
LNDMAILVRASFQMREFEDRFVTLGLNYRVIGGPRFYERLEIRDAMAYFRLVCQPADDLAFERIVNTPKRGLGDTTIRNL
HDYARARDIPMLAAAADIIETDELKPKARKALFDVVQDFRRWQGLLENTEHTTLAEQILDESGYTAMWQADKTAEAPGRL
ENLKELIRSMESFESMRGFLEHVALVMDAEQNAELDAVSIMTLHSAKGLEFDTVFLPGWEEGLFPHQRALDEGGRSGLEE
ERRLAYVGITRAKKLCHIWFVSNRRIHGLWQSTLPSRFLDELPPAHVDVAASDSNYGGYGGRGGYGQSRFDKADPFTNNY
STPGWKRAQQNRSDATRDNWGTRSGHAVERIGYGESGPRTRTIDGELVAKSVADKPSKFFVGDRVFHLKFGNGNISAIEG
NKLTINFDRAGEKRVLDGFVEKV

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=653, Percent_Identity=43.1852986217458, Blast_Score=489, Evalue=1e-139,
Organism=Escherichia coli, GI48994965, Length=650, Percent_Identity=40, Blast_Score=397, Evalue=1e-111,
Organism=Escherichia coli, GI1787196, Length=347, Percent_Identity=28.2420749279539, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322369, Length=513, Percent_Identity=30.2144249512671, Blast_Score=182, Evalue=2e-46,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 92590; Mature: 92458

Theoretical pI: Translated: 6.49; Mature: 6.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSFDDMPFFDEEPVAPRRATNNAPSENGGGLGIAARAMAARDQVRPAHDYLSGLNPEQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCHHH
REAVETLDGPVLVLAGAGTGKTRVLTTRIAHILATGRAYPSQILAVTFTNKAAREMKERI
HHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHH
GVLVGGAVEGMPWLGTFHSIGVKLLRRHAELIGLKSDFTILDTDDVVRLIKQLIQAEGLD
CEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHCCCC
DKRWPAKQFAGMIDGWKNKGLTPPDIPEGDSRAFANGKGRELYAAYQARLKTLNACDFGD
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHH
LLLHPISIFRRHTDILKEYHQKFRYILVDEYQDTNTAQYMWLRLLAQRAKDEPQNVCCVG
HHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEC
DDDQSIYGWRGAEVDNILRFDKDFPGAKVIKLERNYRSTEHILGAAGHLIAHNEGRLGKT
CCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCEEECCCCCCCHH
LFTERSSPDDEKVVVHAAWDSEEEARAVGEEIEQLQRKNYKLNDMAILVRASFQMREFED
HHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHCC
RFVTLGLNYRVIGGPRFYERLEIRDAMAYFRLVCQPADDLAFERIVNTPKRGLGDTTIRN
CEEEEECCEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHH
LHDYARARDIPMLAAAADIIETDELKPKARKALFDVVQDFRRWQGLLENTEHTTLAEQIL
HHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
DESGYTAMWQADKTAEAPGRLENLKELIRSMESFESMRGFLEHVALVMDAEQNAELDAVS
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
IMTLHSAKGLEFDTVFLPGWEEGLFPHQRALDEGGRSGLEEERRLAYVGITRAKKLCHIW
EEEEECCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCHHHHHEEEEEHHHHHHEEEEE
FVSNRRIHGLWQSTLPSRFLDELPPAHVDVAASDSNYGGYGGRGGYGQSRFDKADPFTNN
EECCCEEEHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
YSTPGWKRAQQNRSDATRDNWGTRSGHAVERIGYGESGPRTRTIDGELVAKSVADKPSKF
CCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEECCHHHHHHHHHCCCCEE
FVGDRVFHLKFGNGNISAIEGNKLTINFDRAGEKRVLDGFVEKV
EECCEEEEEEECCCCEEEEECCEEEEEECCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SNSFDDMPFFDEEPVAPRRATNNAPSENGGGLGIAARAMAARDQVRPAHDYLSGLNPEQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCHHH
REAVETLDGPVLVLAGAGTGKTRVLTTRIAHILATGRAYPSQILAVTFTNKAAREMKERI
HHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHH
GVLVGGAVEGMPWLGTFHSIGVKLLRRHAELIGLKSDFTILDTDDVVRLIKQLIQAEGLD
CEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHCCCC
DKRWPAKQFAGMIDGWKNKGLTPPDIPEGDSRAFANGKGRELYAAYQARLKTLNACDFGD
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHH
LLLHPISIFRRHTDILKEYHQKFRYILVDEYQDTNTAQYMWLRLLAQRAKDEPQNVCCVG
HHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEC
DDDQSIYGWRGAEVDNILRFDKDFPGAKVIKLERNYRSTEHILGAAGHLIAHNEGRLGKT
CCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCEEECCCCCCCHH
LFTERSSPDDEKVVVHAAWDSEEEARAVGEEIEQLQRKNYKLNDMAILVRASFQMREFED
HHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHCC
RFVTLGLNYRVIGGPRFYERLEIRDAMAYFRLVCQPADDLAFERIVNTPKRGLGDTTIRN
CEEEEECCEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHH
LHDYARARDIPMLAAAADIIETDELKPKARKALFDVVQDFRRWQGLLENTEHTTLAEQIL
HHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
DESGYTAMWQADKTAEAPGRLENLKELIRSMESFESMRGFLEHVALVMDAEQNAELDAVS
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
IMTLHSAKGLEFDTVFLPGWEEGLFPHQRALDEGGRSGLEEERRLAYVGITRAKKLCHIW
EEEEECCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCHHHHHEEEEEHHHHHHEEEEE
FVSNRRIHGLWQSTLPSRFLDELPPAHVDVAASDSNYGGYGGRGGYGQSRFDKADPFTNN
EECCCEEEHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
YSTPGWKRAQQNRSDATRDNWGTRSGHAVERIGYGESGPRTRTIDGELVAKSVADKPSKF
CCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEECCHHHHHHHHHCCCCEE
FVGDRVFHLKFGNGNISAIEGNKLTINFDRAGEKRVLDGFVEKV
EECCEEEEEEECCCCEEEEECCEEEEEECCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA