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The map label for this gene is pdxJ

Identifier: 159185015

GI number: 159185015

Start: 1985658

End: 1986413

Strand: Reverse

Name: pdxJ

Synonym: Atu2024

Alternate gene names: 159185015

Gene position: 1986413-1985658 (Counterclockwise)

Preceding gene: 159185016

Following gene: 159185014

Centisome position: 69.91

GC content: 59.79

Gene sequence:

>756_bases
ATGCCTGCAAAACTTTCCGTCAATCTGAACGCAATTGCCATGTTACGCAACCGCCGCGATCTTCCCTGGCCTGATGTCGC
GCATTTCGGGCAGATTGCGCTCTCGGCGGGTGCAAGCGGGTTGACTGTACACCCGCGGCCCGACCAGCGCCATATCCGCT
TTTCGGATCTGCCGGTGCTGCGGGCGCTGATCGATGATCGTTTCCCCGGCGCGGAATTCAACATCGAAGGGTATCCCACC
GAGGATTTTCTCGTTCTCTGCGAAAAGACCCAGCCGGAACAGGTGACGCTGGTGCCTGACGACCCGTCGCAGGCGACGTC
AGACCACGGCTGGGATTTCCGCAAACATGCTGTTTTTCTGAAAGATGTCGTCGGGCGCCTGAAGGCCGGTGGCATGCGCG
TTTCACTGTTTGCCGATGGCGACGGGGAACGCGAGCCGGTGGAGCTGGCGGCCGAGACGGGGGCCGCCCGCATCGAGCTT
TACACCGGCCCTTATGGCGGCTGTTTTGACGATACGCAAAAGGCTGACCTGCTTGTCGAGAAGCTTGGGCAGACGGCGGA
TCATGCCGCAGCACTCGGGCTTGCTGTCAATGCCGGCCATGATCTGACGGTTGCGAACCTGCCCAAGCTGATGAAACGTA
TCCCCAATCTCGCTGAGGTATCGATCGGCCACGGGCTGACGGCAGATGCCATGGAATATGGCATGGCTGAAACAGTCCGG
CGTTTTTGTCAGGCCTGCGGTCAGCGTAATTCATAA

Upstream 100 bases:

>100_bases
GCTTTCGTTCCCGCAGCTTTAAAAAATTTTATCGGCCTCCTTGCGGTCGGCGTCAACCTGCGTGAAACAAGGTATCAATT
CCGAACCGGAGCCCGATGCC

Downstream 100 bases:

>100_bases
CCAAAGTCAATTTTTTCGCCTATGCTTTGGCTTGCGCATGCTCCGTGCGCGAGCTTTGGAAGGCAAAGACATATGCAAGA
ACATCATGTTGTCGTCGTGG

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MPAKLSVNLNAIAMLRNRRDLPWPDVAHFGQIALSAGASGLTVHPRPDQRHIRFSDLPVLRALIDDRFPGAEFNIEGYPT
EDFLVLCEKTQPEQVTLVPDDPSQATSDHGWDFRKHAVFLKDVVGRLKAGGMRVSLFADGDGEREPVELAAETGAARIEL
YTGPYGGCFDDTQKADLLVEKLGQTADHAAALGLAVNAGHDLTVANLPKLMKRIPNLAEVSIGHGLTADAMEYGMAETVR
RFCQACGQRNS

Sequences:

>Translated_251_residues
MPAKLSVNLNAIAMLRNRRDLPWPDVAHFGQIALSAGASGLTVHPRPDQRHIRFSDLPVLRALIDDRFPGAEFNIEGYPT
EDFLVLCEKTQPEQVTLVPDDPSQATSDHGWDFRKHAVFLKDVVGRLKAGGMRVSLFADGDGEREPVELAAETGAARIEL
YTGPYGGCFDDTQKADLLVEKLGQTADHAAALGLAVNAGHDLTVANLPKLMKRIPNLAEVSIGHGLTADAMEYGMAETVR
RFCQACGQRNS
>Mature_250_residues
PAKLSVNLNAIAMLRNRRDLPWPDVAHFGQIALSAGASGLTVHPRPDQRHIRFSDLPVLRALIDDRFPGAEFNIEGYPTE
DFLVLCEKTQPEQVTLVPDDPSQATSDHGWDFRKHAVFLKDVVGRLKAGGMRVSLFADGDGEREPVELAAETGAARIELY
TGPYGGCFDDTQKADLLVEKLGQTADHAAALGLAVNAGHDLTVANLPKLMKRIPNLAEVSIGHGLTADAMEYGMAETVRR
FCQACGQRNS

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=235, Percent_Identity=38.7234042553192, Blast_Score=148, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_AGRT5 (Q8UDU5)

Other databases:

- EMBL:   AE007869
- PIR:   A97603
- PIR:   AB2825
- RefSeq:   NP_354993.2
- ProteinModelPortal:   Q8UDU5
- SMR:   Q8UDU5
- STRING:   Q8UDU5
- GeneID:   1134062
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2024
- eggNOG:   COG0854
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- PhylomeDB:   Q8UDU5
- ProtClustDB:   PRK05265
- BioCyc:   ATUM176299-1:ATU2024-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 27283; Mature: 27152

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: ACT_SITE 44-44 ACT_SITE 76-76 ACT_SITE 200-200 BINDING 8-8 BINDING 19-19 BINDING 46-46 BINDING 51-51 BINDING 106-106 BINDING 201-201

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAKLSVNLNAIAMLRNRRDLPWPDVAHFGQIALSAGASGLTVHPRPDQRHIRFSDLPVL
CCCEEECCHHHEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEECCCCCCCCEEEHHHHHH
RALIDDRFPGAEFNIEGYPTEDFLVLCEKTQPEQVTLVPDDPSQATSDHGWDFRKHAVFL
HHHHHCCCCCCEEEECCCCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHH
KDVVGRLKAGGMRVSLFADGDGEREPVELAAETGAARIELYTGPYGGCFDDTQKADLLVE
HHHHHHHHCCCEEEEEEECCCCCCCHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHH
KLGQTADHAAALGLAVNAGHDLTVANLPKLMKRIPNLAEVSIGHGLTADAMEYGMAETVR
HHCCCHHHHHHHEEEEECCCCCCHHHHHHHHHHCCCHHEECCCCCCCHHHHHHHHHHHHH
RFCQACGQRNS
HHHHHHCCCCC
>Mature Secondary Structure 
PAKLSVNLNAIAMLRNRRDLPWPDVAHFGQIALSAGASGLTVHPRPDQRHIRFSDLPVL
CCEEECCHHHEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEECCCCCCCCEEEHHHHHH
RALIDDRFPGAEFNIEGYPTEDFLVLCEKTQPEQVTLVPDDPSQATSDHGWDFRKHAVFL
HHHHHCCCCCCEEEECCCCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHH
KDVVGRLKAGGMRVSLFADGDGEREPVELAAETGAARIELYTGPYGGCFDDTQKADLLVE
HHHHHHHHCCCEEEEEEECCCCCCCHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHH
KLGQTADHAAALGLAVNAGHDLTVANLPKLMKRIPNLAEVSIGHGLTADAMEYGMAETVR
HHCCCHHHHHHHEEEEECCCCCCHHHHHHHHHHCCCHHEECCCCCCCHHHHHHHHHHHHH
RFCQACGQRNS
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194