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The map label for this gene is lasT [H]

Identifier: 159184955

GI number: 159184955

Start: 1846590

End: 1847423

Strand: Reverse

Name: lasT [H]

Synonym: Atu1868

Alternate gene names: 159184955

Gene position: 1847423-1846590 (Counterclockwise)

Preceding gene: 159184956

Following gene: 15889167

Centisome position: 65.01

GC content: 60.67

Gene sequence:

>834_bases
ATGGCAGGCACTAACAGCGAGCTTGAACTTGTGGCGGAAGGCCCGGCGATCATTCTGGTCGAACCGCAGCTCGGCGAAAA
TATCGGCATGGTGGCGCGGGCCATGGCCAATTTCGGCCTTTCCGAATTGCGTCTGGTCAATCCGCGTGATGGCTGGCCCA
GCGAGAAGGCGCGCGCCGCCGCTTCCAAGGCCGACCACGTCATTGACGGCACCAGGGTGTTCGAGACGCTGGAAGAGGCG
ATCAAGGATCTCAATTTCGTCTATGCCACGACCGCGCGCGAGCGTTACGGGTTCAAGCCGGTGCGGGCGCCCGTCACCGC
CGCCGATACGCTCAGGACAAAATTCAAGGCTGGCGAAAAGACCGGTATCCTGTTCGGGCGTGAGCGCTGGGGGCTGACGA
ACGAGGAGGTGGCGCTGGCCGACGAGATCGTGACCTTCCCGGTCAATCCTGCCTTTGCCTCGCTCAACATCGCGCAGGCC
GTGCTTTTGATGTCCTATGAATGGATGAAGTCTGGCATGGACGATCTGGGTGAGACGCTGTTCCAGCCCATCGAGCAACG
GCCCTCGACCAAGGAGCAGGTTTTTGGCCTGTTCGAACATATCGAGGAAGCGCTGGATGCGCGCGGTTATTTCCACCCTG
CTGAAAAAAAGCCGAAAATGGTCGACAATCTGCGGGCGGTGCTGTCGAGACGCGGCTTTTCCGAGCAGGAAATCAGTGTT
TTCCGCGGCGTGATCAGGTCGCTCGATCGTTTTCCCCGGCAGTGGCCAAAGAAGACGGGCAGGGCAGCGGGGCCGTTGGC
CGAGGGAGCAGGGGAGAATGCTGAAGACGAGTGA

Upstream 100 bases:

>100_bases
CCCCTCCGGCTTTGATTTTCGCGCTGATCTGTGCCAGTGAGGCGCGCTTTAGGGCATCGTTGAAAAATCGATGCCGCGCT
TCAAACACGGACAAATGACG

Downstream 100 bases:

>100_bases
GGCGGCGGCCGATGTGCTGAAACCGGTGCTGGTGTTCGATTCCGGCATTGGTGGGCTGACGGTTCTGCGCGAGGCGCGGG
TGCTGATGCCCGAGCGCGGT

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MAGTNSELELVAEGPAIILVEPQLGENIGMVARAMANFGLSELRLVNPRDGWPSEKARAAASKADHVIDGTRVFETLEEA
IKDLNFVYATTARERYGFKPVRAPVTAADTLRTKFKAGEKTGILFGRERWGLTNEEVALADEIVTFPVNPAFASLNIAQA
VLLMSYEWMKSGMDDLGETLFQPIEQRPSTKEQVFGLFEHIEEALDARGYFHPAEKKPKMVDNLRAVLSRRGFSEQEISV
FRGVIRSLDRFPRQWPKKTGRAAGPLAEGAGENAEDE

Sequences:

>Translated_277_residues
MAGTNSELELVAEGPAIILVEPQLGENIGMVARAMANFGLSELRLVNPRDGWPSEKARAAASKADHVIDGTRVFETLEEA
IKDLNFVYATTARERYGFKPVRAPVTAADTLRTKFKAGEKTGILFGRERWGLTNEEVALADEIVTFPVNPAFASLNIAQA
VLLMSYEWMKSGMDDLGETLFQPIEQRPSTKEQVFGLFEHIEEALDARGYFHPAEKKPKMVDNLRAVLSRRGFSEQEISV
FRGVIRSLDRFPRQWPKKTGRAAGPLAEGAGENAEDE
>Mature_276_residues
AGTNSELELVAEGPAIILVEPQLGENIGMVARAMANFGLSELRLVNPRDGWPSEKARAAASKADHVIDGTRVFETLEEAI
KDLNFVYATTARERYGFKPVRAPVTAADTLRTKFKAGEKTGILFGRERWGLTNEEVALADEIVTFPVNPAFASLNIAQAV
LLMSYEWMKSGMDDLGETLFQPIEQRPSTKEQVFGLFEHIEEALDARGYFHPAEKKPKMVDNLRAVLSRRGFSEQEISVF
RGVIRSLDRFPRQWPKKTGRAAGPLAEGAGENAEDE

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=242, Percent_Identity=34.297520661157, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1790865, Length=151, Percent_Identity=40.3973509933775, Blast_Score=107, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30696; Mature: 30565

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGTNSELELVAEGPAIILVEPQLGENIGMVARAMANFGLSELRLVNPRDGWPSEKARAA
CCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHCCHHHEEEECCCCCCCCHHHHHH
ASKADHVIDGTRVFETLEEAIKDLNFVYATTARERYGFKPVRAPVTAADTLRTKFKAGEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHCCCCCCCCCCHHHHHHHHHHHCCCC
TGILFGRERWGLTNEEVALADEIVTFPVNPAFASLNIAQAVLLMSYEWMKSGMDDLGETL
CCEEEEHHHCCCCCCHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
FQPIEQRPSTKEQVFGLFEHIEEALDARGYFHPAEKKPKMVDNLRAVLSRRGFSEQEISV
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
FRGVIRSLDRFPRQWPKKTGRAAGPLAEGAGENAEDE
HHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCC
>Mature Secondary Structure 
AGTNSELELVAEGPAIILVEPQLGENIGMVARAMANFGLSELRLVNPRDGWPSEKARAA
CCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHCCHHHEEEECCCCCCCCHHHHHH
ASKADHVIDGTRVFETLEEAIKDLNFVYATTARERYGFKPVRAPVTAADTLRTKFKAGEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHCCCCCCCCCCHHHHHHHHHHHCCCC
TGILFGRERWGLTNEEVALADEIVTFPVNPAFASLNIAQAVLLMSYEWMKSGMDDLGETL
CCEEEEHHHCCCCCCHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
FQPIEQRPSTKEQVFGLFEHIEEALDARGYFHPAEKKPKMVDNLRAVLSRRGFSEQEISV
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
FRGVIRSLDRFPRQWPKKTGRAAGPLAEGAGENAEDE
HHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA