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The map label for this gene is kefA [C]

Identifier: 159184847

GI number: 159184847

Start: 1620412

End: 1622919

Strand: Reverse

Name: kefA [C]

Synonym: Atu1633

Alternate gene names: 159184847

Gene position: 1622919-1620412 (Counterclockwise)

Preceding gene: 15888947

Following gene: 15888944

Centisome position: 57.11

GC content: 61.2

Gene sequence:

>2508_bases
ATGCCGGCCCTTGCGCAGGAAACAACGACGCCGGCAGCGCCGGGCGCCATCCAGGCCTCTACGGTCGAGCAAACGAGATC
AGATCTCGAAAAATGGAAGACGGACATCGCGTTCATCTCGGGGCAGGTGGAGGCGGGTGGACGCGACGACGTCCAACTGG
TTGACCTCAAGGGGCGGGCCGATGGCATCGCTGCCGATGCCGCCGCCGCCAATGCCAAACTTCGGGCACGGCTGGACCAG
ATCAAGACCCGGCTGGATGCACTGGGTGCGGCACCAGGCGAAGGCCAGCCGCCGGAGGCAAGCCTGGTGACGGAAGAACG
AAACCGGCTGACGGCGGAGCGCGGCGAGGTGAATGCCATGGCGGGCGAAGTGGAGGCCACCGCCAACAATGCCGCGCAGA
TTTCCAACAATATCACGGCGGTTCGCCGGGCTCTTTTTGCCGCCACCCTGTTCAAGCGCACGGAAGTTTCCGCCCAGACG
CTGGGCGACGCATCCTCCGCCTTTCTGGCCGAGCTTACCAATCTCAACAATGCCTTCAGCAACTGGACGGCCTATGTCTG
GAACTACAAGCGGTTGCCGATGTTCGGGGCCGTGATGCTGTCGATCATGGCGGCGCTGTTGTTTCTGGTCGGCGGTTACC
GCTTTTTCGGCAGCCGCATGGACCGGCGTGCCTTTACGGGCGAGCCTTCTTATCTCAGAAGGCTTTCGGTGGCTTTCTGG
TCAACCATGGTGCAGTCTCTGTCGCTGTTCCTGTTTCTCGTCACTTCGGCCTTCTTCCTCGATAATTTCAACGTATTGCG
GTCCGACATAGCGCCGATCCTGTTCGGGGCGATGGCGATCACCGGTTTTGTTTATTTCGTCTCGCGACTGAGCTATGCGA
TCTTTGCGCCGACGCAGCCGGAATGGCGCCTGCTGAAGGTGTCCAACAAGGGCGCGCATACGCTGTCTTCGGCGGTGCTG
CTGATGGCGCTCGTCAACGGTCTCGATTATTTGTTCGGCACGATCAGCGAGACGCTTTATTCGCCGCTGATCGTCACCGT
CGCCAAGAGTTTCATCGCGTCCATCATCATCGGGCTCATTCTGCTCACCGTATCGTTCCTGCGGCCGATGATCGGCGAGG
AACAGGATTACGACACCGGCAATCAGCGCCTGCCGCGCTGGCTCGTCATTCTGCTGCGGGTGGGTGGCCTCATCCTCATC
GGCGCTTGCCTGACCGGTTATGTCGGGCTTGCGCGTTTCCTCGCCACTCAGATCGTCGCGACCGGTGCGGTGCTGGCGAC
CATGTATATCGGCATTCTCTCCGGCAAGGCGATTTCGCGGCAGGGTGCCTTTGGCGAATCCCTTGCCGGGCGTTATCTCG
CGCGGCGTTTCAGCCTCGGTCCGGTTGCGCTCGATCAGGCCGGCCTTGCTGCAGGGCTCGGCATATATGTCGTGGCGCTT
GCTTTCGGCGTGCCGCTCATCCTGTTTTCCTGGGGTTTCCAGCCGGGCGATATCGAAAGCTGGGCCTATCGCCTGCTGAC
CGGCATCACCGTCGGCAATGCCTCCATCTCGCTGATCGGTCTGTTCGGCGGCGTCCTCGTCTTCGCCATCGGCTATATCA
TCACCCGCTGGTTCCAGAAGTGGCTGGACAATAACGTCATGGCGCGGGGCCAGGTGGATGCCGGGGTGCGCAACTCGGTC
AAAACAGGCATCGGTTATCTCGGCATCGCCGTTGCGGCCATCTTTGGCGTCTCCTCCGCCGGACTCAATCTTTCAAGCCT
GGCGCTGGTCGCTTCGGCCCTTTCGGTCGGTATCGGTTTTGGTCTGCAGAACATCGTCTCGAATTTCGTCTCGGGCCTCA
TCCTCCTGGTCGAACGCCCGTTCAAGGTGGGAGACTGGGTGGTGACCGGCACGACGGAGGGAACCGTCAAGCGGCTTTCC
GTGCGCGCCACCGAAATCGAAACTTTTCGCGGCCAGTCGATCATCGTGCCGAATTCGGAATTCATCAATTCTTCTGTCGG
GAACTGGACGCACCGCAACCGCATCATGCGGGCGGAAATTCCGGTTTCCGTGGCTTATGATTCCGATCCGCAGCAGGTGA
TGGATATTTTGCTGGAGCTGGTGCGCGCCCAGCCGCCGGTGCTGCGCAACCCCGAGCCGCATGTGGAATTCCTGCGTTTC
GGCGATTTTTCGCTGGATTTCGAATTGCGTTTCCACCTTGCCGATCTTTCGAACGGGCTGGCTGTAAAAAATGCGCTGCG
GATCGCCATCCTCCACCGTTTCCGTGAAGAGGGCATCGCCATCCCGTTCCCGCAGCGCAACCTCAACATCCATGTGGAAG
GCGACGCCAATCCGCAGATGCTGGCGGCGCTGCTTTCGGAAGAGGGCGACAAGGCCGTGGTGGGCCATACAGGGGCGGCT
GCCGCGCCGACTAGCAGCGCGACGCTCCCAAAAGACGGTCCTGAGACCGACGATGAGGCGGCTCCCGCAAACGCTGCAAC
CGCCAAGACCCCCGGCAAGGCCAAATGA

Upstream 100 bases:

>100_bases
CGGACGGGTGTGGCGCGGGCGAGCGGTTGCTGGCAGGCGAGGTGGCGGCATGGCCCCGATTCTGGTCGTTCTTTTTGCCC
TCCTCAGCAGCCTTTGGGTG

Downstream 100 bases:

>100_bases
TGATCACCGACATGGTCGTGTCTGCGACAGCAATTTCCTCCCTGTCGCAGCACCCCTTGGCCATGTCGCAATCAGGCGGC
TGAACGGCAGGCAAGCGCTT

Product: potassium efflux system KEFA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 835; Mature: 834

Protein sequence:

>835_residues
MPALAQETTTPAAPGAIQASTVEQTRSDLEKWKTDIAFISGQVEAGGRDDVQLVDLKGRADGIAADAAAANAKLRARLDQ
IKTRLDALGAAPGEGQPPEASLVTEERNRLTAERGEVNAMAGEVEATANNAAQISNNITAVRRALFAATLFKRTEVSAQT
LGDASSAFLAELTNLNNAFSNWTAYVWNYKRLPMFGAVMLSIMAALLFLVGGYRFFGSRMDRRAFTGEPSYLRRLSVAFW
STMVQSLSLFLFLVTSAFFLDNFNVLRSDIAPILFGAMAITGFVYFVSRLSYAIFAPTQPEWRLLKVSNKGAHTLSSAVL
LMALVNGLDYLFGTISETLYSPLIVTVAKSFIASIIIGLILLTVSFLRPMIGEEQDYDTGNQRLPRWLVILLRVGGLILI
GACLTGYVGLARFLATQIVATGAVLATMYIGILSGKAISRQGAFGESLAGRYLARRFSLGPVALDQAGLAAGLGIYVVAL
AFGVPLILFSWGFQPGDIESWAYRLLTGITVGNASISLIGLFGGVLVFAIGYIITRWFQKWLDNNVMARGQVDAGVRNSV
KTGIGYLGIAVAAIFGVSSAGLNLSSLALVASALSVGIGFGLQNIVSNFVSGLILLVERPFKVGDWVVTGTTEGTVKRLS
VRATEIETFRGQSIIVPNSEFINSSVGNWTHRNRIMRAEIPVSVAYDSDPQQVMDILLELVRAQPPVLRNPEPHVEFLRF
GDFSLDFELRFHLADLSNGLAVKNALRIAILHRFREEGIAIPFPQRNLNIHVEGDANPQMLAALLSEEGDKAVVGHTGAA
AAPTSSATLPKDGPETDDEAAPANAATAKTPGKAK

Sequences:

>Translated_835_residues
MPALAQETTTPAAPGAIQASTVEQTRSDLEKWKTDIAFISGQVEAGGRDDVQLVDLKGRADGIAADAAAANAKLRARLDQ
IKTRLDALGAAPGEGQPPEASLVTEERNRLTAERGEVNAMAGEVEATANNAAQISNNITAVRRALFAATLFKRTEVSAQT
LGDASSAFLAELTNLNNAFSNWTAYVWNYKRLPMFGAVMLSIMAALLFLVGGYRFFGSRMDRRAFTGEPSYLRRLSVAFW
STMVQSLSLFLFLVTSAFFLDNFNVLRSDIAPILFGAMAITGFVYFVSRLSYAIFAPTQPEWRLLKVSNKGAHTLSSAVL
LMALVNGLDYLFGTISETLYSPLIVTVAKSFIASIIIGLILLTVSFLRPMIGEEQDYDTGNQRLPRWLVILLRVGGLILI
GACLTGYVGLARFLATQIVATGAVLATMYIGILSGKAISRQGAFGESLAGRYLARRFSLGPVALDQAGLAAGLGIYVVAL
AFGVPLILFSWGFQPGDIESWAYRLLTGITVGNASISLIGLFGGVLVFAIGYIITRWFQKWLDNNVMARGQVDAGVRNSV
KTGIGYLGIAVAAIFGVSSAGLNLSSLALVASALSVGIGFGLQNIVSNFVSGLILLVERPFKVGDWVVTGTTEGTVKRLS
VRATEIETFRGQSIIVPNSEFINSSVGNWTHRNRIMRAEIPVSVAYDSDPQQVMDILLELVRAQPPVLRNPEPHVEFLRF
GDFSLDFELRFHLADLSNGLAVKNALRIAILHRFREEGIAIPFPQRNLNIHVEGDANPQMLAALLSEEGDKAVVGHTGAA
AAPTSSATLPKDGPETDDEAAPANAATAKTPGKAK
>Mature_834_residues
PALAQETTTPAAPGAIQASTVEQTRSDLEKWKTDIAFISGQVEAGGRDDVQLVDLKGRADGIAADAAAANAKLRARLDQI
KTRLDALGAAPGEGQPPEASLVTEERNRLTAERGEVNAMAGEVEATANNAAQISNNITAVRRALFAATLFKRTEVSAQTL
GDASSAFLAELTNLNNAFSNWTAYVWNYKRLPMFGAVMLSIMAALLFLVGGYRFFGSRMDRRAFTGEPSYLRRLSVAFWS
TMVQSLSLFLFLVTSAFFLDNFNVLRSDIAPILFGAMAITGFVYFVSRLSYAIFAPTQPEWRLLKVSNKGAHTLSSAVLL
MALVNGLDYLFGTISETLYSPLIVTVAKSFIASIIIGLILLTVSFLRPMIGEEQDYDTGNQRLPRWLVILLRVGGLILIG
ACLTGYVGLARFLATQIVATGAVLATMYIGILSGKAISRQGAFGESLAGRYLARRFSLGPVALDQAGLAAGLGIYVVALA
FGVPLILFSWGFQPGDIESWAYRLLTGITVGNASISLIGLFGGVLVFAIGYIITRWFQKWLDNNVMARGQVDAGVRNSVK
TGIGYLGIAVAAIFGVSSAGLNLSSLALVASALSVGIGFGLQNIVSNFVSGLILLVERPFKVGDWVVTGTTEGTVKRLSV
RATEIETFRGQSIIVPNSEFINSSVGNWTHRNRIMRAEIPVSVAYDSDPQQVMDILLELVRAQPPVLRNPEPHVEFLRFG
DFSLDFELRFHLADLSNGLAVKNALRIAILHRFREEGIAIPFPQRNLNIHVEGDANPQMLAALLSEEGDKAVVGHTGAAA
APTSSATLPKDGPETDDEAAPANAATAKTPGKAK

Specific function: Unknown

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=284, Percent_Identity=35.9154929577465, Blast_Score=159, Evalue=9e-40,
Organism=Escherichia coli, GI2367355, Length=249, Percent_Identity=31.7269076305221, Blast_Score=138, Evalue=2e-33,
Organism=Escherichia coli, GI1789291, Length=257, Percent_Identity=28.4046692607004, Blast_Score=109, Evalue=8e-25,
Organism=Escherichia coli, GI1787591, Length=197, Percent_Identity=27.4111675126904, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 89603; Mature: 89472

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPALAQETTTPAAPGAIQASTVEQTRSDLEKWKTDIAFISGQVEAGGRDDVQLVDLKGRA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECEECCCCCCCEEEEEECCCC
DGIAADAAAANAKLRARLDQIKTRLDALGAAPGEGQPPEASLVTEERNRLTAERGEVNAM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHH
AGEVEATANNAAQISNNITAVRRALFAATLFKRTEVSAQTLGDASSAFLAELTNLNNAFS
HCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
NWTAYVWNYKRLPMFGAVMLSIMAALLFLVGGYRFFGSRMDRRAFTGEPSYLRRLSVAFW
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
STMVQSLSLFLFLVTSAFFLDNFNVLRSDIAPILFGAMAITGFVYFVSRLSYAIFAPTQP
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCC
EWRLLKVSNKGAHTLSSAVLLMALVNGLDYLFGTISETLYSPLIVTVAKSFIASIIIGLI
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLTVSFLRPMIGEEQDYDTGNQRLPRWLVILLRVGGLILIGACLTGYVGLARFLATQIVA
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGAVLATMYIGILSGKAISRQGAFGESLAGRYLARRFSLGPVALDQAGLAAGLGIYVVAL
HHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
AFGVPLILFSWGFQPGDIESWAYRLLTGITVGNASISLIGLFGGVLVFAIGYIITRWFQK
HHHHHHHHHHCCCCCCCHHHHHHHHHHHCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WLDNNVMARGQVDAGVRNSVKTGIGYLGIAVAAIFGVSSAGLNLSSLALVASALSVGIGF
HHCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC
GLQNIVSNFVSGLILLVERPFKVGDWVVTGTTEGTVKRLSVRATEIETFRGQSIIVPNSE
CHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHEEEHHHHHHHCCCEEECCCHH
FINSSVGNWTHRNRIMRAEIPVSVAYDSDPQQVMDILLELVRAQPPVLRNPEPHVEFLRF
HHCCCCCCCHHHCCEEEEECCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEE
GDFSLDFELRFHLADLSNGLAVKNALRIAILHRFREEGIAIPFPQRNLNIHVEGDANPQM
CCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHH
LAALLSEEGDKAVVGHTGAAAAPTSSATLPKDGPETDDEAAPANAATAKTPGKAK
HHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PALAQETTTPAAPGAIQASTVEQTRSDLEKWKTDIAFISGQVEAGGRDDVQLVDLKGRA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECEECCCCCCCEEEEEECCCC
DGIAADAAAANAKLRARLDQIKTRLDALGAAPGEGQPPEASLVTEERNRLTAERGEVNAM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHH
AGEVEATANNAAQISNNITAVRRALFAATLFKRTEVSAQTLGDASSAFLAELTNLNNAFS
HCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
NWTAYVWNYKRLPMFGAVMLSIMAALLFLVGGYRFFGSRMDRRAFTGEPSYLRRLSVAFW
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
STMVQSLSLFLFLVTSAFFLDNFNVLRSDIAPILFGAMAITGFVYFVSRLSYAIFAPTQP
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCC
EWRLLKVSNKGAHTLSSAVLLMALVNGLDYLFGTISETLYSPLIVTVAKSFIASIIIGLI
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLTVSFLRPMIGEEQDYDTGNQRLPRWLVILLRVGGLILIGACLTGYVGLARFLATQIVA
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGAVLATMYIGILSGKAISRQGAFGESLAGRYLARRFSLGPVALDQAGLAAGLGIYVVAL
HHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
AFGVPLILFSWGFQPGDIESWAYRLLTGITVGNASISLIGLFGGVLVFAIGYIITRWFQK
HHHHHHHHHHCCCCCCCHHHHHHHHHHHCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WLDNNVMARGQVDAGVRNSVKTGIGYLGIAVAAIFGVSSAGLNLSSLALVASALSVGIGF
HHCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC
GLQNIVSNFVSGLILLVERPFKVGDWVVTGTTEGTVKRLSVRATEIETFRGQSIIVPNSE
CHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHEEEHHHHHHHCCCEEECCCHH
FINSSVGNWTHRNRIMRAEIPVSVAYDSDPQQVMDILLELVRAQPPVLRNPEPHVEFLRF
HHCCCCCCCHHHCCEEEEECCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEE
GDFSLDFELRFHLADLSNGLAVKNALRIAILHRFREEGIAIPFPQRNLNIHVEGDANPQM
CCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHH
LAALLSEEGDKAVVGHTGAAAAPTSSATLPKDGPETDDEAAPANAATAKTPGKAK
HHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]