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The map label for this gene is gyrB [H]

Identifier: 159184842

GI number: 159184842

Start: 1606994

End: 1609126

Strand: Direct

Name: gyrB [H]

Synonym: Atu1622

Alternate gene names: 159184842

Gene position: 1606994-1609126 (Clockwise)

Preceding gene: 15888930

Following gene: 159184843

Centisome position: 56.55

GC content: 61.42

Gene sequence:

>2133_bases
ATGATGGACGACAGCAACGATCTCTTCTCCGGTCTTCCGGCAGCACAAAACAGCGATACGGAAACGAACGAGGACACGGT
GAAACCCGTGAAGGCCCCGGCGACCGCAGCTGCCAACGTGCCCGCCAACCGGAATGTGCAGCCGAAACCCGCAGCCATTG
CGGCCATCCCAACGTCCGCTCCGCCCGCCCCCGTGTCTCCTTCCAGTGATGACTACGGCGCCTCGTCGATCCGCGTGCTT
GAAGGGCTGGAGCCCGTTCGCATGCGTCCGGGCATGTATATTGGCGGCACCGACGAAAAGGCGCTGCACCATCTCTTTGC
CGAAGTCATCGACAACTCCATGGACGAGGCCGTCGCAGGCCACGCCAATTTCATCGAGGTTCATCTCGATGCTGAAGGTT
TTCTGACCGTCACCGATAACGGCCGCGGTATCCCCGTGGAAAACCATCCGCAGGTTCCGGGCAAATCGACGCTCGAAGTC
ATCATGACCAAGCTGCATGCCGGCGGCAAATTCGACGGCAAGGCCTATGAGACATCGGGCGGCCTGCATGGCGTCGGCGT
TTCCGTGGTCAATGCGCTCTCCGACCTGCTGGAAGTTGAAGTCGCCCGTAACCGCAAGCTTTACCGCCAGCGTTTTTCGC
GCGGCGCGCCGATTGGCGGGCTTGAGGAACTGGGCGACGTGCATAATCGCCGCGGCACCCGCGTGCGTTTCCACCCCGAC
CCGCAGATTTTCGGCGATCACGCGAAATTCGAACCGGCCCGCATCTTCCGCATGGCCCGCTCCAAGGCCTATCTGTTCGG
CGGCGTAGAAATCCGCTGGAGTTGCGACCCCGGCATGGTGCCGGCCGATGGCGAAATCCCTGAAAAGGCCGTGTTCCATT
TTCCGGGTGGTCTCAAGGACTATCTGGCGGCCACGCTCGGCAAGGAATTCACGGTCACCCGCGAGATTTTCTCGGGCCGC
ACCGAAAAGACCGGCGGCCACGGTGCGTTGGAATGGGCTGTCACCTGGTATGGCGGCGACACGCAGATCCATTCCTATTG
CAACACCATCCCGACGCCCGAAGGCGGCACGCATGAGGCCGGTTTCCGCATCGCGCTCACCAAGGGCCTGAAGAACTATG
CCGAGTTGACGCAGAACAAGCGCGCCCGTGAAATCACCACCGATGACGTGATGATCTCGGCAGCCGGCATGCTCTCGGTC
TTTATCCGCGAGCCGGAATTCGTCGGCCAGACCAAGGACAAGCTTGCGACCGTCGAGGCCCAGCGCATTGTTGAAAACGC
GCTGCGCGATCCCTTCGACCACTATCTCACCGGCAACCCGGGCGAAGCGGCCAAGCTGCTCGACTGGGTGATCGAGCGCG
CCGAAGAACGCCTTCGCCGCCGCAAGGAAAAGGAAGTCAACCGCAAGACGGCGGTGCGCAAGCTGCGCCTGCCCGGCAAG
CTGGCGGACTGCTCGCAGAACACGGCTGAGAATGCGGAACTTTTCATCGTCGAGGGTGACTCGGCTGGCGGCTCGGCCAA
GCAGGCGCGCAACCGCGCCAATCAGGCCATCCTGCCGCTGCGTGGCAAGATTTTGAACGTCGGCAGCGCCAGCCGCGAAA
AGCTATCGGCCAACCAGCAGATCGCCGATCTCATCCAGGCACTCGGCTGCGGCACGCGCACCAAGTACCGCGAAGAGGAT
CTGCGTTACGAACGCATCATCATCATGACCGATGCCGATGTCGACGGCGCCCACATCGCCTCGCTGCTCATCACCTTTTT
CTATCAGGAAATGCCGGAACTCATCCGCGGCAACCATCTCTATCTGGCCGTGCCGCCGCTCTACGTCATCCGTCAGGGTG
CCAAAAGCGCCTATGCCCGCGACGACGCTCATCGCGCAGAGCTGATGGAAACCGTCTTTAAGGGCAAGAAGGTCGAAATC
GGCCGCTTCAAAGGCCTCGGTGAAATGATGGCCGCACAGCTGAAGGAAACCACCATGGATCCCGAAAAGCGCACCCTGCT
GCGCGTCGAGATCGATGACGTGGATTTCGAAGGCACCCGCGAGGCGGTGGACAATCTGATGGGCACCAAGGCGGATGCTC
GCTTCCGTTTCATTCAGGAACGGGCGGCTTTCGCGGATAATCTGGATATTTGA

Upstream 100 bases:

>100_bases
CGGTTCAATCGGACGGGAAACGCATTTGGCGTTATGATTGACGCGATTGACATAATGATTCGCATGGCGAAATTCTGACA
CACGCTTTTGAACTTTGGAC

Downstream 100 bases:

>100_bases
TGAGAAACCCAAGCTAGTCTTGGCCAAATAGCCCTAATACTGGCCATTTTGGAACCTGAAACTGGCCAACGCCACGTTTG
ATTATTTCCGGGCACTGAAG

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 710; Mature: 710

Protein sequence:

>710_residues
MMDDSNDLFSGLPAAQNSDTETNEDTVKPVKAPATAAANVPANRNVQPKPAAIAAIPTSAPPAPVSPSSDDYGASSIRVL
EGLEPVRMRPGMYIGGTDEKALHHLFAEVIDNSMDEAVAGHANFIEVHLDAEGFLTVTDNGRGIPVENHPQVPGKSTLEV
IMTKLHAGGKFDGKAYETSGGLHGVGVSVVNALSDLLEVEVARNRKLYRQRFSRGAPIGGLEELGDVHNRRGTRVRFHPD
PQIFGDHAKFEPARIFRMARSKAYLFGGVEIRWSCDPGMVPADGEIPEKAVFHFPGGLKDYLAATLGKEFTVTREIFSGR
TEKTGGHGALEWAVTWYGGDTQIHSYCNTIPTPEGGTHEAGFRIALTKGLKNYAELTQNKRAREITTDDVMISAAGMLSV
FIREPEFVGQTKDKLATVEAQRIVENALRDPFDHYLTGNPGEAAKLLDWVIERAEERLRRRKEKEVNRKTAVRKLRLPGK
LADCSQNTAENAELFIVEGDSAGGSAKQARNRANQAILPLRGKILNVGSASREKLSANQQIADLIQALGCGTRTKYREED
LRYERIIIMTDADVDGAHIASLLITFFYQEMPELIRGNHLYLAVPPLYVIRQGAKSAYARDDAHRAELMETVFKGKKVEI
GRFKGLGEMMAAQLKETTMDPEKRTLLRVEIDDVDFEGTREAVDNLMGTKADARFRFIQERAAFADNLDI

Sequences:

>Translated_710_residues
MMDDSNDLFSGLPAAQNSDTETNEDTVKPVKAPATAAANVPANRNVQPKPAAIAAIPTSAPPAPVSPSSDDYGASSIRVL
EGLEPVRMRPGMYIGGTDEKALHHLFAEVIDNSMDEAVAGHANFIEVHLDAEGFLTVTDNGRGIPVENHPQVPGKSTLEV
IMTKLHAGGKFDGKAYETSGGLHGVGVSVVNALSDLLEVEVARNRKLYRQRFSRGAPIGGLEELGDVHNRRGTRVRFHPD
PQIFGDHAKFEPARIFRMARSKAYLFGGVEIRWSCDPGMVPADGEIPEKAVFHFPGGLKDYLAATLGKEFTVTREIFSGR
TEKTGGHGALEWAVTWYGGDTQIHSYCNTIPTPEGGTHEAGFRIALTKGLKNYAELTQNKRAREITTDDVMISAAGMLSV
FIREPEFVGQTKDKLATVEAQRIVENALRDPFDHYLTGNPGEAAKLLDWVIERAEERLRRRKEKEVNRKTAVRKLRLPGK
LADCSQNTAENAELFIVEGDSAGGSAKQARNRANQAILPLRGKILNVGSASREKLSANQQIADLIQALGCGTRTKYREED
LRYERIIIMTDADVDGAHIASLLITFFYQEMPELIRGNHLYLAVPPLYVIRQGAKSAYARDDAHRAELMETVFKGKKVEI
GRFKGLGEMMAAQLKETTMDPEKRTLLRVEIDDVDFEGTREAVDNLMGTKADARFRFIQERAAFADNLDI
>Mature_710_residues
MMDDSNDLFSGLPAAQNSDTETNEDTVKPVKAPATAAANVPANRNVQPKPAAIAAIPTSAPPAPVSPSSDDYGASSIRVL
EGLEPVRMRPGMYIGGTDEKALHHLFAEVIDNSMDEAVAGHANFIEVHLDAEGFLTVTDNGRGIPVENHPQVPGKSTLEV
IMTKLHAGGKFDGKAYETSGGLHGVGVSVVNALSDLLEVEVARNRKLYRQRFSRGAPIGGLEELGDVHNRRGTRVRFHPD
PQIFGDHAKFEPARIFRMARSKAYLFGGVEIRWSCDPGMVPADGEIPEKAVFHFPGGLKDYLAATLGKEFTVTREIFSGR
TEKTGGHGALEWAVTWYGGDTQIHSYCNTIPTPEGGTHEAGFRIALTKGLKNYAELTQNKRAREITTDDVMISAAGMLSV
FIREPEFVGQTKDKLATVEAQRIVENALRDPFDHYLTGNPGEAAKLLDWVIERAEERLRRRKEKEVNRKTAVRKLRLPGK
LADCSQNTAENAELFIVEGDSAGGSAKQARNRANQAILPLRGKILNVGSASREKLSANQQIADLIQALGCGTRTKYREED
LRYERIIIMTDADVDGAHIASLLITFFYQEMPELIRGNHLYLAVPPLYVIRQGAKSAYARDDAHRAELMETVFKGKKVEI
GRFKGLGEMMAAQLKETTMDPEKRTLLRVEIDDVDFEGTREAVDNLMGTKADARFRFIQERAAFADNLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=617, Percent_Identity=25.9319286871961, Blast_Score=148, Evalue=2e-35,
Organism=Homo sapiens, GI19913408, Length=628, Percent_Identity=25, Blast_Score=133, Evalue=5e-31,
Organism=Escherichia coli, GI48994957, Length=560, Percent_Identity=44.8214285714286, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI1789408, Length=641, Percent_Identity=40.7176287051482, Blast_Score=434, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI212645845, Length=600, Percent_Identity=23.8333333333333, Blast_Score=122, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17535065, Length=620, Percent_Identity=23.5483870967742, Blast_Score=122, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI212645657, Length=209, Percent_Identity=26.3157894736842, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=678, Percent_Identity=25.0737463126844, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17136538, Length=646, Percent_Identity=24.7678018575851, Blast_Score=130, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 78057; Mature: 78057

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDDSNDLFSGLPAAQNSDTETNEDTVKPVKAPATAAANVPANRNVQPKPAAIAAIPTSA
CCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCC
PPAPVSPSSDDYGASSIRVLEGLEPVRMRPGMYIGGTDEKALHHLFAEVIDNSMDEAVAG
CCCCCCCCCCCCCCCHHHHHHCCCCHHCCCCEEECCCCHHHHHHHHHHHHCCHHHHHHCC
HANFIEVHLDAEGFLTVTDNGRGIPVENHPQVPGKSTLEVIMTKLHAGGKFDGKAYETSG
CCEEEEEEECCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCC
GLHGVGVSVVNALSDLLEVEVARNRKLYRQRFSRGAPIGGLEELGDVHNRRGTRVRFHPD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEECCC
PQIFGDHAKFEPARIFRMARSKAYLFGGVEIRWSCDPGMVPADGEIPEKAVFHFPGGLKD
CCCCCCCCCCCHHHHHHHHHCCEEEEECEEEEECCCCCCCCCCCCCCCHHEEECCCCHHH
YLAATLGKEFTVTREIFSGRTEKTGGHGALEWAVTWYGGDTQIHSYCNTIPTPEGGTHEA
HHHHHHCCCCEEHHHHHCCCCCCCCCCCEEEEEEEEECCCHHHHHHHCCCCCCCCCCCCC
GFRIALTKGLKNYAELTQNKRAREITTDDVMISAAGMLSVFIREPEFVGQTKDKLATVEA
CEEEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHH
QRIVENALRDPFDHYLTGNPGEAAKLLDWVIERAEERLRRRKEKEVNRKTAVRKLRLPGK
HHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LADCSQNTAENAELFIVEGDSAGGSAKQARNRANQAILPLRGKILNVGSASREKLSANQQ
HHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEECCCEEEECCCCHHHHHHHHHH
IADLIQALGCGTRTKYREEDLRYERIIIMTDADVDGAHIASLLITFFYQEMPELIRGNHL
HHHHHHHHCCCCCCCHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEE
YLAVPPLYVIRQGAKSAYARDDAHRAELMETVFKGKKVEIGRFKGLGEMMAAQLKETTMD
EEECCHHHHHHHCHHHHHHCCHHHHHHHHHHHHCCCCEEEHHHCCHHHHHHHHHHHHCCC
PEKRTLLRVEIDDVDFEGTREAVDNLMGTKADARFRFIQERAAFADNLDI
CCCCEEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMDDSNDLFSGLPAAQNSDTETNEDTVKPVKAPATAAANVPANRNVQPKPAAIAAIPTSA
CCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCC
PPAPVSPSSDDYGASSIRVLEGLEPVRMRPGMYIGGTDEKALHHLFAEVIDNSMDEAVAG
CCCCCCCCCCCCCCCHHHHHHCCCCHHCCCCEEECCCCHHHHHHHHHHHHCCHHHHHHCC
HANFIEVHLDAEGFLTVTDNGRGIPVENHPQVPGKSTLEVIMTKLHAGGKFDGKAYETSG
CCEEEEEEECCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCC
GLHGVGVSVVNALSDLLEVEVARNRKLYRQRFSRGAPIGGLEELGDVHNRRGTRVRFHPD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEECCC
PQIFGDHAKFEPARIFRMARSKAYLFGGVEIRWSCDPGMVPADGEIPEKAVFHFPGGLKD
CCCCCCCCCCCHHHHHHHHHCCEEEEECEEEEECCCCCCCCCCCCCCCHHEEECCCCHHH
YLAATLGKEFTVTREIFSGRTEKTGGHGALEWAVTWYGGDTQIHSYCNTIPTPEGGTHEA
HHHHHHCCCCEEHHHHHCCCCCCCCCCCEEEEEEEEECCCHHHHHHHCCCCCCCCCCCCC
GFRIALTKGLKNYAELTQNKRAREITTDDVMISAAGMLSVFIREPEFVGQTKDKLATVEA
CEEEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHH
QRIVENALRDPFDHYLTGNPGEAAKLLDWVIERAEERLRRRKEKEVNRKTAVRKLRLPGK
HHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LADCSQNTAENAELFIVEGDSAGGSAKQARNRANQAILPLRGKILNVGSASREKLSANQQ
HHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEECCCEEEECCCCHHHHHHHHHH
IADLIQALGCGTRTKYREEDLRYERIIIMTDADVDGAHIASLLITFFYQEMPELIRGNHL
HHHHHHHHCCCCCCCHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEE
YLAVPPLYVIRQGAKSAYARDDAHRAELMETVFKGKKVEIGRFKGLGEMMAAQLKETTMD
EEECCHHHHHHHCHHHHHHCCHHHHHHHHHHHHCCCCEEEHHHCCHHHHHHHHHHHHCCC
PEKRTLLRVEIDDVDFEGTREAVDNLMGTKADARFRFIQERAAFADNLDI
CCCCEEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9797224 [H]