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The map label for this gene is proS

Identifier: 159184694

GI number: 159184694

Start: 1275738

End: 1277060

Strand: Direct

Name: proS

Synonym: Atu1288

Alternate gene names: 159184694

Gene position: 1275738-1277060 (Clockwise)

Preceding gene: 17935187

Following gene: 15888617

Centisome position: 44.9

GC content: 57.75

Gene sequence:

>1323_bases
ATGCGTCTTAGCCGTTATTTCCTGCCCATCCTGAAGGAAAACCCCAAGGAAGCCGAGATTGTTTCTCACCGCCTCATGCT
GCGCGCCGGCATGATCCGGCAGCAATCGGCTGGCATCTATTCCTGGTTGCCGCTCGGCAAGCGCGTGCTTGATAAGGTCA
ACAAGATCATCCGCGAGGAGCAGAACCGTGCCGGCGCCATCGAGCTTCTGATGCCGACGCTGCAAACGGCCGAACTCTGG
CAGGAAAGCGGTCGTTACGACGATTACGGCAAGGAAATGCTGCGTATCAAGGACCGCCAGGACCGCCAGATGCTCTACGG
CCCCACCAATGAGGAGATGATCACTGACATCTTCCGTTCCTATGTGAAGTCCTACAAGAACCTGCCGCTGAACCTCTATC
ATATCCAGCTAAAGTTCCGCGACGAGGTGCGTCCGCGTTTCGGCACCATGCGCTCGCGTGAGTTCCTGATGAAGGATGCT
TATTCCTTCGACCTGACCAAGGAAGACGCGATCCATTCCTATAACAAGATGTTCGTGGCCTATCTGCGCACCTTCGAGCG
CCTTGGCCTGCGCGCTATTCCGATGCGCGCCGATACCGGTCCGATCGGCGGCAACCACAGCCATGAATTCATCATTCTGG
CCGATACCGGCGAATCCGAAGTCTTCTGCCACAAGAGCTTCCTTGACCGCGCCATCCCGGCCGAAAGCACCGATTTCGAC
GATGTCGCGGCGCTGCAGGGCGTGTTCGACGAGTGGACGGCCGATTACGCCGCGACGTCTGAAATGCACGACGATGCTGC
CTACGATGCCATTCCCGAAGGCGAGCGCCTTTCCGCGCGCGGCATCGAGGTTGGCCACATCTTCTATTTCGGCACCAAAT
ATTCCGAGCCGATGGGCGCGAAAGTGCAGGGCAAGGATGGCAAGGAACACCCTGTCCACATGGGTTCCTATGGCATTGGA
CCGACACGCCTTGTTCCCGCCATCATTGAAGCATCGCATGACGAGAATGGAATCATCTGGCCGGCCTCGGTCGCTCCTTT
CGATGTCGTGATCATCAACATGAAGGCTGGCGATGCGGCCTGCGATGCGGCTTGTGAAAAGCTGTATTATCAGCTCTCCA
ACGCCGGCAAGGATGTTCTCTACGACGATACCGACGACCGTGCAGGCCAGAAATTCGCCACAGCCGATCTGATCGGTGTG
CCGGTGCAGATCATCGTCGGCCCGCGTTCTGTTGCGAACGGCGAAGTCGAAGTGAAGGACCGCAAGACCGGCGAACGTGA
AACCGTCACAATCGAGGCGGCAATGAACAAGGCGCTTGGCTAA

Upstream 100 bases:

>100_bases
TTCTTCTTGGCGGGAAACGGCTATATCCGGCGCCAATATCCCTCTATTCGGGGCGATTCCCGCGCTTATGGCGCTGTCGT
CCGTATCAATGGAAGCCGTC

Downstream 100 bases:

>100_bases
GCGCAAAGGGAAAGATGGGAGCGGCCTTCATGCGAAGACCGCTCCGGTTTTATGGCGCAGGCGCTGCTACGATTGGGACC
GATCTTTGGAAAAGACCGAT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MRLSRYFLPILKENPKEAEIVSHRLMLRAGMIRQQSAGIYSWLPLGKRVLDKVNKIIREEQNRAGAIELLMPTLQTAELW
QESGRYDDYGKEMLRIKDRQDRQMLYGPTNEEMITDIFRSYVKSYKNLPLNLYHIQLKFRDEVRPRFGTMRSREFLMKDA
YSFDLTKEDAIHSYNKMFVAYLRTFERLGLRAIPMRADTGPIGGNHSHEFIILADTGESEVFCHKSFLDRAIPAESTDFD
DVAALQGVFDEWTADYAATSEMHDDAAYDAIPEGERLSARGIEVGHIFYFGTKYSEPMGAKVQGKDGKEHPVHMGSYGIG
PTRLVPAIIEASHDENGIIWPASVAPFDVVIINMKAGDAACDAACEKLYYQLSNAGKDVLYDDTDDRAGQKFATADLIGV
PVQIIVGPRSVANGEVEVKDRKTGERETVTIEAAMNKALG

Sequences:

>Translated_440_residues
MRLSRYFLPILKENPKEAEIVSHRLMLRAGMIRQQSAGIYSWLPLGKRVLDKVNKIIREEQNRAGAIELLMPTLQTAELW
QESGRYDDYGKEMLRIKDRQDRQMLYGPTNEEMITDIFRSYVKSYKNLPLNLYHIQLKFRDEVRPRFGTMRSREFLMKDA
YSFDLTKEDAIHSYNKMFVAYLRTFERLGLRAIPMRADTGPIGGNHSHEFIILADTGESEVFCHKSFLDRAIPAESTDFD
DVAALQGVFDEWTADYAATSEMHDDAAYDAIPEGERLSARGIEVGHIFYFGTKYSEPMGAKVQGKDGKEHPVHMGSYGIG
PTRLVPAIIEASHDENGIIWPASVAPFDVVIINMKAGDAACDAACEKLYYQLSNAGKDVLYDDTDDRAGQKFATADLIGV
PVQIIVGPRSVANGEVEVKDRKTGERETVTIEAAMNKALG
>Mature_440_residues
MRLSRYFLPILKENPKEAEIVSHRLMLRAGMIRQQSAGIYSWLPLGKRVLDKVNKIIREEQNRAGAIELLMPTLQTAELW
QESGRYDDYGKEMLRIKDRQDRQMLYGPTNEEMITDIFRSYVKSYKNLPLNLYHIQLKFRDEVRPRFGTMRSREFLMKDA
YSFDLTKEDAIHSYNKMFVAYLRTFERLGLRAIPMRADTGPIGGNHSHEFIILADTGESEVFCHKSFLDRAIPAESTDFD
DVAALQGVFDEWTADYAATSEMHDDAAYDAIPEGERLSARGIEVGHIFYFGTKYSEPMGAKVQGKDGKEHPVHMGSYGIG
PTRLVPAIIEASHDENGIIWPASVAPFDVVIINMKAGDAACDAACEKLYYQLSNAGKDVLYDDTDDRAGQKFATADLIGV
PVQIIVGPRSVANGEVEVKDRKTGERETVTIEAAMNKALG

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=437, Percent_Identity=36.3844393592677, Blast_Score=256, Evalue=4e-68,
Organism=Escherichia coli, GI1786392, Length=221, Percent_Identity=59.2760180995475, Blast_Score=284, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI115532348, Length=399, Percent_Identity=33.0827067669173, Blast_Score=208, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI193203271, Length=97, Percent_Identity=35.0515463917526, Blast_Score=78, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI71984184, Length=415, Percent_Identity=21.6867469879518, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71984192, Length=415, Percent_Identity=21.6867469879518, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6320931, Length=225, Percent_Identity=36.8888888888889, Blast_Score=163, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24656200, Length=424, Percent_Identity=32.5471698113208, Blast_Score=226, Evalue=3e-59,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_AGRT5 (Q8UFV9)

Other databases:

- EMBL:   AE007869
- PIR:   A97516
- PIR:   AH2734
- RefSeq:   NP_354297.2
- ProteinModelPortal:   Q8UFV9
- SMR:   Q8UFV9
- STRING:   Q8UFV9
- GeneID:   1133326
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu1288
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   Q8UFV9
- ProtClustDB:   PRK12325
- BioCyc:   ATUM176299-1:ATU1288-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01570
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.15

Molecular weight: Translated: 49610; Mature: 49610

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSRYFLPILKENPKEAEIVSHRLMLRAGMIRQQSAGIYSWLPLGKRVLDKVNKIIREE
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
QNRAGAIELLMPTLQTAELWQESGRYDDYGKEMLRIKDRQDRQMLYGPTNEEMITDIFRS
CCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHH
YVKSYKNLPLNLYHIQLKFRDEVRPRFGTMRSREFLMKDAYSFDLTKEDAIHSYNKMFVA
HHHHHHCCCEEEEEEEEEECCCCCHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
YLRTFERLGLRAIPMRADTGPIGGNHSHEFIILADTGESEVFCHKSFLDRAIPAESTDFD
HHHHHHHCCCEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEEEHHHHHHCCCCCCCCHH
DVAALQGVFDEWTADYAATSEMHDDAAYDAIPEGERLSARGIEVGHIFYFGTKYSEPMGA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCC
KVQGKDGKEHPVHMGSYGIGPTRLVPAIIEASHDENGIIWPASVAPFDVVIINMKAGDAA
EEECCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEEEECCCCHH
CDAACEKLYYQLSNAGKDVLYDDTDDRAGQKFATADLIGVPVQIIVGPRSVANGEVEVKD
HHHHHHHHHHHHHCCCCCCEECCCCCCCCCEEEHHHHCCCCEEEEECCCCCCCCEEEEEC
RKTGERETVTIEAAMNKALG
CCCCCCEEEEEHHHHHHCCC
>Mature Secondary Structure
MRLSRYFLPILKENPKEAEIVSHRLMLRAGMIRQQSAGIYSWLPLGKRVLDKVNKIIREE
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
QNRAGAIELLMPTLQTAELWQESGRYDDYGKEMLRIKDRQDRQMLYGPTNEEMITDIFRS
CCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHH
YVKSYKNLPLNLYHIQLKFRDEVRPRFGTMRSREFLMKDAYSFDLTKEDAIHSYNKMFVA
HHHHHHCCCEEEEEEEEEECCCCCHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
YLRTFERLGLRAIPMRADTGPIGGNHSHEFIILADTGESEVFCHKSFLDRAIPAESTDFD
HHHHHHHCCCEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEEEHHHHHHCCCCCCCCHH
DVAALQGVFDEWTADYAATSEMHDDAAYDAIPEGERLSARGIEVGHIFYFGTKYSEPMGA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCC
KVQGKDGKEHPVHMGSYGIGPTRLVPAIIEASHDENGIIWPASVAPFDVVIINMKAGDAA
EEECCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEEEECCCCHH
CDAACEKLYYQLSNAGKDVLYDDTDDRAGQKFATADLIGVPVQIIVGPRSVANGEVEVKD
HHHHHHHHHHHHHCCCCCCEECCCCCCCCCEEEHHHHCCCCEEEEECCCCCCCCEEEEEC
RKTGERETVTIEAAMNKALG
CCCCCCEEEEEHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194