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The map label for this gene is yneF [H]

Identifier: 159184686

GI number: 159184686

Start: 1244567

End: 1245814

Strand: Reverse

Name: yneF [H]

Synonym: Atu1257

Alternate gene names: 159184686

Gene position: 1245814-1244567 (Counterclockwise)

Preceding gene: 159184687

Following gene: 15888585

Centisome position: 43.84

GC content: 60.58

Gene sequence:

>1248_bases
ATGCTGGATGCAAAGACGAGCACGCTACTGTGGGCGGCGGAGACGTTCACCCTTGCCGTGCTTTTGGGAACGCTGTGGCT
GCACCGCCCTTCGCGCCGGCATAATCTCTATTTCGCCGCCGGTTTCCTCGCCACAGGCTTCGGTACCGTCATGGTGGCGC
TTCGCGGGGATATTTCGAGTTTTCTGTCCATTCAGGTCGGCAATACGCTTGCACTTTCCGCCTTCGGTTTCTGGCTCGCG
GGCCTGCTATCTCTGGAGCAGCGCAAACTCGGCGGCTGGATCGCCATTCCGGCGCTATTGTGGATCGCCGGAATGTTCGT
GCCATCAGTGCCTGAAGACATGGTCGCTCGCATCCTCCTTTATCATGCCAGCGCGGCCACGGGATATTTCATGCTCGCCG
GCGTGCTTCTGGGCAGCAGGGCACGTGCTTCACGCAGCCGAAAGGTGCTGGCCACGATCCTGATCCTGCAGGCCTTTGCC
GGAGCGATCGTCGCCTCCATCGTCATCCCGGCCAATGCCGCGGTTCCCAACGCCATCCCCATGACATCGGCGCTCTCCAT
TTCCGGCATGCTCGGCTTTACGGCCATCATCATGATCAGCGCCAAGATCATCATGGAAGACACGGAGATCCGCCTCCACC
GGCTCGCCATGACCGATCACCTGACCGGTGTCCTCAACCGGCGCGGTCTGCTGGAGGAATTCGAACACATCAAGAAGCGC
TCGCACGGCTCCGACCGCTACGTGGCGCTGGTTCTCTTCGACATCGATCATTTCAAGAAGATCAATGACAGATACGGACA
TCAGTCTGGCGATGCCGTGCTTGTGCATTTCTGCAATATCGCACAACGCGTGATCGGCGAGCGCGGCCTTTTCGTCCGGA
TGGGCGGCGAGGAATTTGCGCTGGTGGCCGAAGTCGAGAGCCCGTCCAGCACCGTGACACTCGCGGAGGCCATCCGTAGC
AATCTGCGCCTCTCGAAGATCACCGCACGCAACGAAGCCATCAAGGTGACGACCAGCGTCGGCATATCCGAAGCAATGAT
CAAGGATGCCGACCTGAGCGTGATGATGACCGAGGCCGACCGCGCACTTTATGCCGCCAAGAAAGCGGGGCGCAACCGGA
CCGTCATCCATGTGAATTCGGCCAATGTCGTCGTGCCCGCCCCGGACCGGGACGAAAACCCGATGGACAACAATGCCGAC
CGGCAGGTCGCGGCACTGAACCGTATATCCGCCATCGCCAGCCGGTGA

Upstream 100 bases:

>100_bases
CTTCCGGGTGAAATGGCAGCTCAACAATTCCAGCGGGCATAACGCACCTCGTGCGAAAGGTCCCGCTTTTACCGCATGAT
GGCGCAGATAGGATTTTGCA

Downstream 100 bases:

>100_bases
GGATGTCACCGTACAGCGACAGATGACGGCGAATGCACTGGCCGTTACCCGCCCTTCCCAAAGCGCCACACGCGGACTAA
ACCATCAGCATGACAGCGAC

Product: GGDEF family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MLDAKTSTLLWAAETFTLAVLLGTLWLHRPSRRHNLYFAAGFLATGFGTVMVALRGDISSFLSIQVGNTLALSAFGFWLA
GLLSLEQRKLGGWIAIPALLWIAGMFVPSVPEDMVARILLYHASAATGYFMLAGVLLGSRARASRSRKVLATILILQAFA
GAIVASIVIPANAAVPNAIPMTSALSISGMLGFTAIIMISAKIIMEDTEIRLHRLAMTDHLTGVLNRRGLLEEFEHIKKR
SHGSDRYVALVLFDIDHFKKINDRYGHQSGDAVLVHFCNIAQRVIGERGLFVRMGGEEFALVAEVESPSSTVTLAEAIRS
NLRLSKITARNEAIKVTTSVGISEAMIKDADLSVMMTEADRALYAAKKAGRNRTVIHVNSANVVVPAPDRDENPMDNNAD
RQVAALNRISAIASR

Sequences:

>Translated_415_residues
MLDAKTSTLLWAAETFTLAVLLGTLWLHRPSRRHNLYFAAGFLATGFGTVMVALRGDISSFLSIQVGNTLALSAFGFWLA
GLLSLEQRKLGGWIAIPALLWIAGMFVPSVPEDMVARILLYHASAATGYFMLAGVLLGSRARASRSRKVLATILILQAFA
GAIVASIVIPANAAVPNAIPMTSALSISGMLGFTAIIMISAKIIMEDTEIRLHRLAMTDHLTGVLNRRGLLEEFEHIKKR
SHGSDRYVALVLFDIDHFKKINDRYGHQSGDAVLVHFCNIAQRVIGERGLFVRMGGEEFALVAEVESPSSTVTLAEAIRS
NLRLSKITARNEAIKVTTSVGISEAMIKDADLSVMMTEADRALYAAKKAGRNRTVIHVNSANVVVPAPDRDENPMDNNAD
RQVAALNRISAIASR
>Mature_415_residues
MLDAKTSTLLWAAETFTLAVLLGTLWLHRPSRRHNLYFAAGFLATGFGTVMVALRGDISSFLSIQVGNTLALSAFGFWLA
GLLSLEQRKLGGWIAIPALLWIAGMFVPSVPEDMVARILLYHASAATGYFMLAGVLLGSRARASRSRKVLATILILQAFA
GAIVASIVIPANAAVPNAIPMTSALSISGMLGFTAIIMISAKIIMEDTEIRLHRLAMTDHLTGVLNRRGLLEEFEHIKKR
SHGSDRYVALVLFDIDHFKKINDRYGHQSGDAVLVHFCNIAQRVIGERGLFVRMGGEEFALVAEVESPSSTVTLAEAIRS
NLRLSKITARNEAIKVTTSVGISEAMIKDADLSVMMTEADRALYAAKKAGRNRTVIHVNSANVVVPAPDRDENPMDNNAD
RQVAALNRISAIASR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787802, Length=223, Percent_Identity=36.7713004484305, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI87082007, Length=189, Percent_Identity=33.8624338624339, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788085, Length=167, Percent_Identity=35.9281437125749, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1786584, Length=170, Percent_Identity=32.3529411764706, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787262, Length=167, Percent_Identity=34.7305389221557, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787816, Length=206, Percent_Identity=31.5533980582524, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI145693134, Length=167, Percent_Identity=34.7305389221557, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787541, Length=172, Percent_Identity=31.9767441860465, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI87081881, Length=171, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788381, Length=183, Percent_Identity=27.8688524590164, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081977, Length=156, Percent_Identity=29.4871794871795, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI87081974, Length=161, Percent_Identity=29.1925465838509, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788956, Length=171, Percent_Identity=30.4093567251462, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 45004; Mature: 45004

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDAKTSTLLWAAETFTLAVLLGTLWLHRPSRRHNLYFAAGFLATGFGTVMVALRGDISS
CCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCHHH
FLSIQVGNTLALSAFGFWLAGLLSLEQRKLGGWIAIPALLWIAGMFVPSVPEDMVARILL
HEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHCCCCCHHHHHHHHH
YHASAATGYFMLAGVLLGSRARASRSRKVLATILILQAFAGAIVASIVIPANAAVPNAIP
HHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
MTSALSISGMLGFTAIIMISAKIIMEDTEIRLHRLAMTDHLTGVLNRRGLLEEFEHIKKR
CCHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SHGSDRYVALVLFDIDHFKKINDRYGHQSGDAVLVHFCNIAQRVIGERGLFVRMGGEEFA
CCCCCCEEEEEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCEE
LVAEVESPSSTVTLAEAIRSNLRLSKITARNEAIKVTTSVGISEAMIKDADLSVMMTEAD
EEEEECCCCCHHHHHHHHHHCCEEEEEECCCCEEEEEECCCCHHHHHCCCCCEEEEEHHH
RALYAAKKAGRNRTVIHVNSANVVVPAPDRDENPMDNNADRQVAALNRISAIASR
HHHHHHHHCCCCCEEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLDAKTSTLLWAAETFTLAVLLGTLWLHRPSRRHNLYFAAGFLATGFGTVMVALRGDISS
CCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCHHH
FLSIQVGNTLALSAFGFWLAGLLSLEQRKLGGWIAIPALLWIAGMFVPSVPEDMVARILL
HEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHCCCCCHHHHHHHHH
YHASAATGYFMLAGVLLGSRARASRSRKVLATILILQAFAGAIVASIVIPANAAVPNAIP
HHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
MTSALSISGMLGFTAIIMISAKIIMEDTEIRLHRLAMTDHLTGVLNRRGLLEEFEHIKKR
CCHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SHGSDRYVALVLFDIDHFKKINDRYGHQSGDAVLVHFCNIAQRVIGERGLFVRMGGEEFA
CCCCCCEEEEEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCEE
LVAEVESPSSTVTLAEAIRSNLRLSKITARNEAIKVTTSVGISEAMIKDADLSVMMTEAD
EEEEECCCCCHHHHHHHHHHCCEEEEEECCCCEEEEEECCCCHHHHHCCCCCEEEEEHHH
RALYAAKKAGRNRTVIHVNSANVVVPAPDRDENPMDNNADRQVAALNRISAIASR
HHHHHHHHCCCCCEEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]