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The map label for this gene is pksS [H]

Identifier: 15888585

GI number: 15888585

Start: 1243230

End: 1244477

Strand: Reverse

Name: pksS [H]

Synonym: Atu1256

Alternate gene names: 15888585

Gene position: 1244477-1243230 (Counterclockwise)

Preceding gene: 159184686

Following gene: 159184684

Centisome position: 43.8

GC content: 59.78

Gene sequence:

>1248_bases
ATGACAGCGACATTTCCCTTTCTCAAAATCGACCCCGCCACCCGCCGCGTTTCCCTTAACGCCCGCGATCCGGCCTTCTA
CAACGATCCGAACCCGGTTTACGCCGCCCTGCACGCACAATGTCCCACCTTTTACTGGGAAGAGCAGCGGCAATGGTTTT
TCACCTGCTATGACCATGTCAGCACCCTGCTGCGCGATCGCCGGTTCGGGCGGCAGATATTGCATGTCGCAAGTCGTGAA
GAGATCGGCTTGCCGGAGCCGCTTGAGCATGTGAAGCATTTCGACCTGGCCGAGCAGCATTCGCTCCTGGAGCTGGAGCC
GCCGGAGCACACGCGGCTGCGCACGCTGATCAACCGCGCGTTCGTTTCGCGGCATGTCGATAAGATGAAGCCGGAAATCG
AGGAACTCGCCAACCGCCTGATCGAGGCCTTTGAAGCGAATGGCGAGACGGAGCTGCTCTCCTCCTATGCCGACATCATT
CCGGTGACGATGATCGCCCGCATGATCGGCATTCCCGAAGAGATGGGGCCACAGCTTTTGAAGTGGTCGCATGCCTATGT
CGGCATGTACATGTTCAAGCGCACGCCTGAAGACGAGCTTCTGGCCGACAAGGCCGCGCAGGAATTTTCAGACTATGTGC
GCAGAGTCATCGCCGAGCGGCGGGCGGAGCCCAAGGACGATCTGCTGAGCCACATGATCCATACGGAGCACAAGGGCCAG
TACCTCACCGACGACGAGCTGGTCTCCACTACCATCGTGTTATTGAATGCCGGTCATGAGGCGACGGTGCACCAGATCGG
TAATTCCGTGCGCATCATTCTGGAAAGCGGCCTCGACCCCAAGACCCTGTTCCATGACGAGACCGCCACGGAACGCACGG
TCGAGGAAACGCTTCGCATCTGCGCGCCGGTCCACATCTTCCAGCGCTGGGTTCTCGAACCGGTCGAGATCGACGGCGTG
CAGTTCAAGCGTGGCGATAAAGTCAGTCTCATCCTGGCCGCTGCCAACCTCGATCCGGCGAAATTTTCCGATCCTCTGGC
CTTCCAGCCGGATCGGAACGAGGGCGCCAACGTCTCCTTCGGCGCCGGCATCCATTTCTGCATCGGCGCGCCGCTTGCAA
GACTGGAGCTTAATCTCGCCCTGCCCCTGCTTTTCAAACGTCTGCCGGGCCTGAAGATTGCCGAGCCGCCCAAGGTGAAG
GACGTCTATCATTTCCATGGTCTGGAGCGGCTCGATCTGGCGTGGTAG

Upstream 100 bases:

>100_bases
CCAGCCGGTGAGGATGTCACCGTACAGCGACAGATGACGGCGAATGCACTGGCCGTTACCCGCCCTTCCCAAAGCGCCAC
ACGCGGACTAAACCATCAGC

Downstream 100 bases:

>100_bases
ATCGGGGTAACGAAGCTTCGCCTTTAGATCTGCGCCAGCCCACCATCGACGGAAAGTTCCACACCGGTAATATAGCTGGC
CTTGGAAGACGCCAGAAACA

Product: cytochrome P450 hydroxylase

Products: ROH; oxidized flavoprotein; H2O

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MTATFPFLKIDPATRRVSLNARDPAFYNDPNPVYAALHAQCPTFYWEEQRQWFFTCYDHVSTLLRDRRFGRQILHVASRE
EIGLPEPLEHVKHFDLAEQHSLLELEPPEHTRLRTLINRAFVSRHVDKMKPEIEELANRLIEAFEANGETELLSSYADII
PVTMIARMIGIPEEMGPQLLKWSHAYVGMYMFKRTPEDELLADKAAQEFSDYVRRVIAERRAEPKDDLLSHMIHTEHKGQ
YLTDDELVSTTIVLLNAGHEATVHQIGNSVRIILESGLDPKTLFHDETATERTVEETLRICAPVHIFQRWVLEPVEIDGV
QFKRGDKVSLILAAANLDPAKFSDPLAFQPDRNEGANVSFGAGIHFCIGAPLARLELNLALPLLFKRLPGLKIAEPPKVK
DVYHFHGLERLDLAW

Sequences:

>Translated_415_residues
MTATFPFLKIDPATRRVSLNARDPAFYNDPNPVYAALHAQCPTFYWEEQRQWFFTCYDHVSTLLRDRRFGRQILHVASRE
EIGLPEPLEHVKHFDLAEQHSLLELEPPEHTRLRTLINRAFVSRHVDKMKPEIEELANRLIEAFEANGETELLSSYADII
PVTMIARMIGIPEEMGPQLLKWSHAYVGMYMFKRTPEDELLADKAAQEFSDYVRRVIAERRAEPKDDLLSHMIHTEHKGQ
YLTDDELVSTTIVLLNAGHEATVHQIGNSVRIILESGLDPKTLFHDETATERTVEETLRICAPVHIFQRWVLEPVEIDGV
QFKRGDKVSLILAAANLDPAKFSDPLAFQPDRNEGANVSFGAGIHFCIGAPLARLELNLALPLLFKRLPGLKIAEPPKVK
DVYHFHGLERLDLAW
>Mature_414_residues
TATFPFLKIDPATRRVSLNARDPAFYNDPNPVYAALHAQCPTFYWEEQRQWFFTCYDHVSTLLRDRRFGRQILHVASREE
IGLPEPLEHVKHFDLAEQHSLLELEPPEHTRLRTLINRAFVSRHVDKMKPEIEELANRLIEAFEANGETELLSSYADIIP
VTMIARMIGIPEEMGPQLLKWSHAYVGMYMFKRTPEDELLADKAAQEFSDYVRRVIAERRAEPKDDLLSHMIHTEHKGQY
LTDDELVSTTIVLLNAGHEATVHQIGNSVRIILESGLDPKTLFHDETATERTVEETLRICAPVHIFQRWVLEPVEIDGVQ
FKRGDKVSLILAAANLDPAKFSDPLAFQPDRNEGANVSFGAGIHFCIGAPLARLELNLALPLLFKRLPGLKIAEPPKVKD
VYHFHGLERLDLAW

Specific function: Involved in the metabolism of the antibiotic polyketide bacillaene which is involved in secondary metabolism. The substrate is dihydrobacillaene [H]

COG id: COG2124

COG function: function code Q; Cytochrome P450

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome P450 family [H]

Homologues:

Organism=Drosophila melanogaster, GI19920736, Length=217, Percent_Identity=26.2672811059908, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001128
- InterPro:   IPR002397
- InterPro:   IPR017972 [H]

Pfam domain/function: PF00067 p450 [H]

EC number: 1.14.14.1

Molecular weight: Translated: 47459; Mature: 47328

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00086 CYTOCHROME_P450

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATFPFLKIDPATRRVSLNARDPAFYNDPNPVYAALHAQCPTFYWEEQRQWFFTCYDHV
CCCCCCEEEECCCCEEEEECCCCCCCCCCCCCEEEEHHHCCCCCEECCCHHHHHHHHHHH
STLLRDRRFGRQILHVASREEIGLPEPLEHVKHFDLAEQHSLLELEPPEHTRLRTLINRA
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHCCCEECCCCCHHHHHHHHHHH
FVSRHVDKMKPEIEELANRLIEAFEANGETELLSSYADIIPVTMIARMIGIPEEMGPQLL
HHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCHHHH
KWSHAYVGMYMFKRTPEDELLADKAAQEFSDYVRRVIAERRAEPKDDLLSHMIHTEHKGQ
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
YLTDDELVSTTIVLLNAGHEATVHQIGNSVRIILESGLDPKTLFHDETATERTVEETLRI
CCCCHHHHHEEEEEEECCCCHHHHHCCCCEEEEEECCCCHHHEECCCHHHHHHHHHHHHH
CAPVHIFQRWVLEPVEIDGVQFKRGDKVSLILAAANLDPAKFSDPLAFQPDRNEGANVSF
HHHHHHHHHHCCCCEEECCEEECCCCCEEEEEEECCCCHHHCCCCCCCCCCCCCCCCEEE
GAGIHFCIGAPLARLELNLALPLLFKRLPGLKIAEPPKVKDVYHFHGLERLDLAW
CCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCHHCCCC
>Mature Secondary Structure 
TATFPFLKIDPATRRVSLNARDPAFYNDPNPVYAALHAQCPTFYWEEQRQWFFTCYDHV
CCCCCEEEECCCCEEEEECCCCCCCCCCCCCEEEEHHHCCCCCEECCCHHHHHHHHHHH
STLLRDRRFGRQILHVASREEIGLPEPLEHVKHFDLAEQHSLLELEPPEHTRLRTLINRA
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHCCCEECCCCCHHHHHHHHHHH
FVSRHVDKMKPEIEELANRLIEAFEANGETELLSSYADIIPVTMIARMIGIPEEMGPQLL
HHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCHHHH
KWSHAYVGMYMFKRTPEDELLADKAAQEFSDYVRRVIAERRAEPKDDLLSHMIHTEHKGQ
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
YLTDDELVSTTIVLLNAGHEATVHQIGNSVRIILESGLDPKTLFHDETATERTVEETLRI
CCCCHHHHHEEEEEEECCCCHHHHHCCCCEEEEEECCCCHHHEECCCHHHHHHHHHHHHH
CAPVHIFQRWVLEPVEIDGVQFKRGDKVSLILAAANLDPAKFSDPLAFQPDRNEGANVSF
HHHHHHHHHHCCCCEEECCEEECCCCCEEEEEEECCCCHHHCCCCCCCCCCCCCCCCEEE
GAGIHFCIGAPLARLELNLALPLLFKRLPGLKIAEPPKVKDVYHFHGLERLDLAW
CCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RH; reduced flavoprotein; O2

Specific reaction: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]