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The map label for this gene is ogt [H]

Identifier: 159184670

GI number: 159184670

Start: 1214172

End: 1214729

Strand: Direct

Name: ogt [H]

Synonym: Atu1220

Alternate gene names: 159184670

Gene position: 1214172-1214729 (Clockwise)

Preceding gene: 15888555

Following gene: 159184671

Centisome position: 42.73

GC content: 62.01

Gene sequence:

>558_bases
ATGACCAACCATTACGGCTATATTGTTTTTCCTACAGCGCTCGGCCATTGCGGCCTCGCATGGGGAAGGCGTGGCATCGC
CCGTCTGCAATTGCCTAGTCCTGACGCCGGCGAAACCGAAAAGCTTCTCCTGCGACGTCTGCCGTCATCCACGATTGCGG
TGCCGGAAACACGCACGCGCGAAGTGATCGCGATGATCACCCGTTATTTCGGCGGCGAGGCGATCGATTTCACCACCGTG
GAGCTCGATCTGGCCGGGCAGGAGCGCTTCTTCCTCGATGTCTACGCGGTTGCGCGGCAGATCGGCTGGGGCCGCACGAC
GACCTATGGCGGCATCGTGAAAGACCTCGGCCTCAGCATGGAAAAAGCCCGCGATGTCGGTCAGGCCATGGCGAAAAATC
CGGTGCCGCTCATCATTCCCTGCCACCGTGTTCTGGCCGCCGGCGGCAGGCTGGGCGGCTTCTCCGCACCTGGCGGAACC
ACCACCAAATTGCGCATGCTCGATCTTGAAAATGCCGGCCGCAGCCGCGCCGACGAAGCGCAGGGCAGCCTGTTTTAA

Upstream 100 bases:

>100_bases
CCTTATTTTTTTGAGATAGGCTTTTGGGGAGATCAGGAAACGGGTTGTTTCGGACGCAGGATATGGCATCGTGTGCGCCC
GAAAACGGATAATGAATGCG

Downstream 100 bases:

>100_bases
AGCAATGACAGATCAGTGCGCATGAATAGGCGTCTGATTTTTGCCCATTTCTCGTCAAATTGTCGCTAAATTGTCACAAT
ACACGAGCGCCCTCTAACAC

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MTNHYGYIVFPTALGHCGLAWGRRGIARLQLPSPDAGETEKLLLRRLPSSTIAVPETRTREVIAMITRYFGGEAIDFTTV
ELDLAGQERFFLDVYAVARQIGWGRTTTYGGIVKDLGLSMEKARDVGQAMAKNPVPLIIPCHRVLAAGGRLGGFSAPGGT
TTKLRMLDLENAGRSRADEAQGSLF

Sequences:

>Translated_185_residues
MTNHYGYIVFPTALGHCGLAWGRRGIARLQLPSPDAGETEKLLLRRLPSSTIAVPETRTREVIAMITRYFGGEAIDFTTV
ELDLAGQERFFLDVYAVARQIGWGRTTTYGGIVKDLGLSMEKARDVGQAMAKNPVPLIIPCHRVLAAGGRLGGFSAPGGT
TTKLRMLDLENAGRSRADEAQGSLF
>Mature_184_residues
TNHYGYIVFPTALGHCGLAWGRRGIARLQLPSPDAGETEKLLLRRLPSSTIAVPETRTREVIAMITRYFGGEAIDFTTVE
LDLAGQERFFLDVYAVARQIGWGRTTTYGGIVKDLGLSMEKARDVGQAMAKNPVPLIIPCHRVLAAGGRLGGFSAPGGTT
TKLRMLDLENAGRSRADEAQGSLF

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 20050; Mature: 19919

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNHYGYIVFPTALGHCGLAWGRRGIARLQLPSPDAGETEKLLLRRLPSSTIAVPETRTR
CCCCCEEEEECCCCCCCCHHHCCCCEEEEECCCCCCCCHHHHHHHHCCCCCEECCCCHHH
EVIAMITRYFGGEAIDFTTVELDLAGQERFFLDVYAVARQIGWGRTTTYGGIVKDLGLSM
HHHHHHHHHHCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCH
EKARDVGQAMAKNPVPLIIPCHRVLAAGGRLGGFSAPGGTTTKLRMLDLENAGRSRADEA
HHHHHHHHHHHCCCCEEEEECHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCC
QGSLF
CCCCC
>Mature Secondary Structure 
TNHYGYIVFPTALGHCGLAWGRRGIARLQLPSPDAGETEKLLLRRLPSSTIAVPETRTR
CCCCEEEEECCCCCCCCHHHCCCCEEEEECCCCCCCCHHHHHHHHCCCCCEECCCCHHH
EVIAMITRYFGGEAIDFTTVELDLAGQERFFLDVYAVARQIGWGRTTTYGGIVKDLGLSM
HHHHHHHHHHCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCH
EKARDVGQAMAKNPVPLIIPCHRVLAAGGRLGGFSAPGGTTTKLRMLDLENAGRSRADEA
HHHHHHHHHHHCCCCEEEEECHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCC
QGSLF
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]