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The map label for this gene is pleD [H]

Identifier: 159184597

GI number: 159184597

Start: 1050234

End: 1051526

Strand: Reverse

Name: pleD [H]

Synonym: Atu1060

Alternate gene names: 159184597

Gene position: 1051526-1050234 (Counterclockwise)

Preceding gene: 15888403

Following gene: 15888399

Centisome position: 37.0

GC content: 58.93

Gene sequence:

>1293_bases
ATGCAGGATAAAATCCTTCTGATTGAAGATTCCGTTGCGCTTTCCATGCTGCTGAGGACGCGGCTTTCGGATGAAACGGA
AGCCGAGGTCGTCCATTGCGCCAGCATGGCCGAGGCCGATGCCCTGATGCAGGCCAATAATTTCACGCTGGCGCTGACGG
GTCTCAACCTGCCCGATGCGCCCAAGGGGGAAATTCTGACGCTTCTATCCGAGCGCAAGGTGCCGGCCATCGTCTTCACT
GCAACGGTGGATGAGGAAGCGCGCAAGCGTTATGCCGAAAAGAAGATCATCGACTATATCGTCAAGGACGGCCATCGCAC
CGTCGATGCCGTGGTCAAGACGGTCGACCGGATTATGACCAACAAGCGTTTTTCCGTTCTGGTGGTCGATGATGCGCGCA
CCGCACGCTCCGGCCTCGTGGAAATTCTGGAGCGGCAGAATTTCAAGGTCAGCGAAGCCCATTCGGGCAACCGGGCGCTG
GAAATCCTCTCGCAGGACCCGTCAATCCAGCTCGTCATTACCGATTACCACATGCCGGATATGGACGGTTATGAACTGAC
ACGGCGCATCCGCGACAGCAGGTCCTCCGAAGACCTGCGGGTGATCGGCATATCCTCCTCCACGGACCGCCTGCTTTCGG
CAAGCTTCCTCAAGGCCGGCGCGTCGGATTTCGTCTACCGCCCCTTCGTACCGGAAGAATTGCAGTGCCGCATCGACAAC
AATATCGAGACACTGAAGCAGCTCAAGCGCCTGCGTGAACTGGCCGAACGGGACCACCTCACCGGCCTGCCCAATCGCCG
CTCCTTCTTCGAGCGCACGCGCGCGCTGATGGATGTCATCAATGACAATGACGAGAGCGGCGCAGTCGCCATTCTCGACA
TTGACCATTTCAAGAAGATCAACGACACGCTAGGGCACGATGCCGGCGACAGGGCGCTGAAGAAGCTGGCTGAACTGCTG
CAGGGCATGTGCGACGAGCAACGCCATATTCCCGCCCGCCTCGGCGGTGAGGAATTTGCCGTGTTCCTGCGAGGGCTGGA
TGCGCGGGCTGCCTATGCCTTCTGCGAGGAACTGCGCGAACAGGTGGAAAAGAACGGCCGGCAGCTGAGCGGCAGCAGCC
TGGCACTGACAATTTCACTCGGTGTCGTGGAGATCGAAAAGGGCGAGCCCTTCGACAACCAGCTGAATGCGGCGGATCAG
TTGCTCTATCTCGCCAAGGCGAATGGCCGCAACCGCGTTTATTCCGACATCATGATCCAGGAAGGCCTGCAGAAAATCGG
GCTGAACGGCTGA

Upstream 100 bases:

>100_bases
CAATGCATAAGCTGGCCTTCGAATTCGCGGATACGCGAATGACGAATATTCGGCGTTGAGGAAAGACTGGGGAGACGGGC
CAGGGGGGCTTGGGACGGCC

Downstream 100 bases:

>100_bases
AGGCCGCGGCAACGCTCAGCTCTAGTCTCTAGGCACAAAAAACGCCTCAAAACAGCGACATCTGCCCGTCATCCTTCGCC
GGCCTGACCTTCGCCAGCGG

Product: two component response regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MQDKILLIEDSVALSMLLRTRLSDETEAEVVHCASMAEADALMQANNFTLALTGLNLPDAPKGEILTLLSERKVPAIVFT
ATVDEEARKRYAEKKIIDYIVKDGHRTVDAVVKTVDRIMTNKRFSVLVVDDARTARSGLVEILERQNFKVSEAHSGNRAL
EILSQDPSIQLVITDYHMPDMDGYELTRRIRDSRSSEDLRVIGISSSTDRLLSASFLKAGASDFVYRPFVPEELQCRIDN
NIETLKQLKRLRELAERDHLTGLPNRRSFFERTRALMDVINDNDESGAVAILDIDHFKKINDTLGHDAGDRALKKLAELL
QGMCDEQRHIPARLGGEEFAVFLRGLDARAAYAFCEELREQVEKNGRQLSGSSLALTISLGVVEIEKGEPFDNQLNAADQ
LLYLAKANGRNRVYSDIMIQEGLQKIGLNG

Sequences:

>Translated_430_residues
MQDKILLIEDSVALSMLLRTRLSDETEAEVVHCASMAEADALMQANNFTLALTGLNLPDAPKGEILTLLSERKVPAIVFT
ATVDEEARKRYAEKKIIDYIVKDGHRTVDAVVKTVDRIMTNKRFSVLVVDDARTARSGLVEILERQNFKVSEAHSGNRAL
EILSQDPSIQLVITDYHMPDMDGYELTRRIRDSRSSEDLRVIGISSSTDRLLSASFLKAGASDFVYRPFVPEELQCRIDN
NIETLKQLKRLRELAERDHLTGLPNRRSFFERTRALMDVINDNDESGAVAILDIDHFKKINDTLGHDAGDRALKKLAELL
QGMCDEQRHIPARLGGEEFAVFLRGLDARAAYAFCEELREQVEKNGRQLSGSSLALTISLGVVEIEKGEPFDNQLNAADQ
LLYLAKANGRNRVYSDIMIQEGLQKIGLNG
>Mature_430_residues
MQDKILLIEDSVALSMLLRTRLSDETEAEVVHCASMAEADALMQANNFTLALTGLNLPDAPKGEILTLLSERKVPAIVFT
ATVDEEARKRYAEKKIIDYIVKDGHRTVDAVVKTVDRIMTNKRFSVLVVDDARTARSGLVEILERQNFKVSEAHSGNRAL
EILSQDPSIQLVITDYHMPDMDGYELTRRIRDSRSSEDLRVIGISSSTDRLLSASFLKAGASDFVYRPFVPEELQCRIDN
NIETLKQLKRLRELAERDHLTGLPNRRSFFERTRALMDVINDNDESGAVAILDIDHFKKINDTLGHDAGDRALKKLAELL
QGMCDEQRHIPARLGGEEFAVFLRGLDARAAYAFCEELREQVEKNGRQLSGSSLALTISLGVVEIEKGEPFDNQLNAADQ
LLYLAKANGRNRVYSDIMIQEGLQKIGLNG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87082007, Length=182, Percent_Identity=37.3626373626374, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787262, Length=176, Percent_Identity=35.7954545454545, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1786584, Length=166, Percent_Identity=36.7469879518072, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87081881, Length=181, Percent_Identity=34.8066298342541, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787802, Length=170, Percent_Identity=32.3529411764706, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI145693134, Length=175, Percent_Identity=33.7142857142857, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1788381, Length=182, Percent_Identity=31.8681318681319, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=32.9268292682927, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787056, Length=169, Percent_Identity=31.3609467455621, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081977, Length=154, Percent_Identity=34.4155844155844, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787541, Length=205, Percent_Identity=30.2439024390244, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788956, Length=180, Percent_Identity=33.8888888888889, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788191, Length=128, Percent_Identity=33.59375, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1788085, Length=179, Percent_Identity=32.4022346368715, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 48157; Mature: 48157

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00018 EF_HAND_1 ; PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDKILLIEDSVALSMLLRTRLSDETEAEVVHCASMAEADALMQANNFTLALTGLNLPDA
CCCCEEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
PKGEILTLLSERKVPAIVFTATVDEEARKRYAEKKIIDYIVKDGHRTVDAVVKTVDRIMT
CCCHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
NKRFSVLVVDDARTARSGLVEILERQNFKVSEAHSGNRALEILSQDPSIQLVITDYHMPD
CCCEEEEEEECCHHHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCCCEEEEEEECCCCC
MDGYELTRRIRDSRSSEDLRVIGISSSTDRLLSASFLKAGASDFVYRPFVPEELQCRIDN
CCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEECCCCCHHHHEEECC
NIETLKQLKRLRELAERDHLTGLPNRRSFFERTRALMDVINDNDESGAVAILDIDHFKKI
CHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHH
NDTLGHDAGDRALKKLAELLQGMCDEQRHIPARLGGEEFAVFLRGLDARAAYAFCEELRE
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHCCCHHHHHHHHHHHHH
QVEKNGRQLSGSSLALTISLGVVEIEKGEPFDNQLNAADQLLYLAKANGRNRVYSDIMIQ
HHHHCCCEECCCEEEEEEEEEEEEEECCCCCCHHHCHHHHEEEEEECCCCCHHHHHHHHH
EGLQKIGLNG
HHHHHHCCCC
>Mature Secondary Structure
MQDKILLIEDSVALSMLLRTRLSDETEAEVVHCASMAEADALMQANNFTLALTGLNLPDA
CCCCEEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
PKGEILTLLSERKVPAIVFTATVDEEARKRYAEKKIIDYIVKDGHRTVDAVVKTVDRIMT
CCCHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
NKRFSVLVVDDARTARSGLVEILERQNFKVSEAHSGNRALEILSQDPSIQLVITDYHMPD
CCCEEEEEEECCHHHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCCCEEEEEEECCCCC
MDGYELTRRIRDSRSSEDLRVIGISSSTDRLLSASFLKAGASDFVYRPFVPEELQCRIDN
CCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEECCCCCHHHHEEECC
NIETLKQLKRLRELAERDHLTGLPNRRSFFERTRALMDVINDNDESGAVAILDIDHFKKI
CHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHH
NDTLGHDAGDRALKKLAELLQGMCDEQRHIPARLGGEEFAVFLRGLDARAAYAFCEELRE
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHCCCHHHHHHHHHHHHH
QVEKNGRQLSGSSLALTISLGVVEIEKGEPFDNQLNAADQLLYLAKANGRNRVYSDIMIQ
HHHHCCCEECCCEEEEEEEEEEEEEECCCCCCHHHCHHHHEEEEEECCCCCHHHHHHHHH
EGLQKIGLNG
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA