Click here to switch to the map view.

The map label for this gene is slt [H]

Identifier: 159184580

GI number: 159184580

Start: 1015197

End: 1017245

Strand: Reverse

Name: slt [H]

Synonym: Atu1022

Alternate gene names: 159184580

Gene position: 1017245-1015197 (Counterclockwise)

Preceding gene: 15888368

Following gene: 159184579

Centisome position: 35.8

GC content: 64.86

Gene sequence:

>2049_bases
ATGGCGGCGGGGCTTGCCGCAACCGTTTGGAACGCACTGGCCGGACCACTGCCGGAAGGTGCGGCCCCCCTACCCTATGT
CAAGCCAAACGCGCCAGCCGTTCCCGCTTTCATGCCCGCCTCGCCCGAAATCACCGGCGCCATACCGCGCCTCAACCGTC
CTGATGATACCAGCCAGTTGAAGGCCGGCCTCGACGCGCTTTCCAGCCGCGATGCGGCCAGGGCAATCGCGCTGCGCAAC
ACCATGCCGAGCGGCAGCCTCGACCGGCATATTCTCTCTTGGGCGATTGCTGTCTCCGGCCAGAAGGACGTGCCATCAGC
CGAGATCGCCACCGCCCAGCGCGAACTGGCCGGCTGGCCCGGTGCCGCCACCCTGCGGGCGAATTCCGAAAAGGCGCTTT
ACCGGGAAGATCCGCCGGCCGCTCAGGTGCTTTCAGCCTTCGGCAGCAGCCGGCCGGAAACGGCAGAAGGCACGGTCGTG
CTTGGCAAGGCGCTCGCCTCCTCCGGTAAGACAGACGAATCGCAACGTCTCATTCGCCAGCTCTGGGTCAGCGAAGGCAT
GGACAAGGACATGGAAGACCGTGTGCTTGCCGAATTCCCTGCCTATCTGACGCCGGCCGATCACAAGGCACGCATGGATT
ATCTGATGTATCGCAGCCGCATCGGCCAGGCGAAGCGTTTCGGCGATCTCGGCAAGGCGCAGTCGCTTTATAATGCCTGG
GCGGCGGTGCTGCAGCGATCCGCCAATGCCACAGCGTTGCTGGGCAAGGTCGACGCCTCCTGGCGCAACGATCCGACCTA
TCTCTTCGCGCGCATCGAGAACCTGCGCAACCAGCAGAAATATCCCGAAGCCGCCGTCCTTCTGCGGCAGGCGCCGAAGG
AAACGGCCAAGCTCGTCAATCCCGGCGAATGGTGGAATGAGCGCCGCATCATCGCGCGTGGTGTTGCCGATCTCGGCGAT
TTTGCCGAGGCCTATCGCATCGTCGCCAATCATTCGGCCACCTCGTCCGTCGATATTGCCGATGCGGAGTTCCATGCCGG
CTGGTATGCGCTGAGGGCGCTGCGTGATCCGACAACGGCGGCACGCCACTTCAATACGCTGCTGCAGACCTCGAACCGGC
CGCTTTCGGCCTCGCGCGCCTATTATTGGCTTGGACGGACGGCGGAAGCCGGCGGGGCGGGCAATGCCCGTGATTTCTTC
GCCAAGGCAGCCGCCCATCCCGGCACATTCTATGGCCAGCTGGCCGCCGCGCGAATCGGCACGAAGACCCTGAACGTCAG
CTACCCCAACCCCAGCAACGGCGACCGGGAACGTTTTGCCGGCCGCGAGGCCGTGCGCGCCATCGACCGGCTGGAGGCAG
CGGGTTACGGCTGGCGTGCCGACAGTCTTTACCGGTCGCTTGCCGAACAGCTCGACAGCCCCGGCGAGCTAGCCATTCTG
GCAGCGCGGGCGGAAGGCAACGGCAATCATCAGGTTTCACTGCAGATCGGCAAGACGGCTTTCAGCCGCGGCATCGATGC
GGCAGCTCTCGCCTATCCGCTCGGCGTCATTCCGGCCAGCGCCAATATTGCCGGCTCCGGCAAGGCGCTCGCCTATGCCA
TCGCCCGGCAGGAAAGCGCCTTCAACCCGGCCGCCGTCTCCGCCGCCAATGCCCGTGGCCTGCTGCAATTGCTGCCTGGA
ACCGCCAAGGGCGTGGCCGGGCGCCATGGCCTGCCCTACACGCAGGCGATGCTGACCAGCGATGCCGGCTACAACGCCAC
GCTCGGCGCCCACTATCTCGGTGAACAGATCGACAGCTTCGGCGGTTCCTACATCCTCACCTTCGTCGCCTATAATGCGG
GGCCGAAGCGCGTGCCGGAATGGATCTCCCGTTATGGCGACCCGCGCGGCAAGCCCATCGACGAGGTGGTCGACTGGATC
GAGCGCATCCCCTTCCCCGAGACGCGCAATTATGTGCAGCGGGTGATGGAGAATTATCAGGTCTATAAGACCAGGCTCGG
GCAACAGGCCGATATCGTCGATGACCTGCGCCATGGACGTGCGGGGTAA

Upstream 100 bases:

>100_bases
AACCTTCTCGGCAATCCCGCTGCAATGAAGCGATATTACAAGAAAGTCGACAAATCCGTACACGATGAAGAGCGGCATGA
AAAAGACAGTCTGGGGATTA

Downstream 100 bases:

>100_bases
AAGTCGGCTCCATCGTCATCAGAGCATTCAGGCACGGGCTAGCCCGTGCTTTTTTGTTTCAACAGTCAGACGACACGTCG
CATTGCCTCTGGGCCCCCAA

Product: soluble lytic transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 682; Mature: 681

Protein sequence:

>682_residues
MAAGLAATVWNALAGPLPEGAAPLPYVKPNAPAVPAFMPASPEITGAIPRLNRPDDTSQLKAGLDALSSRDAARAIALRN
TMPSGSLDRHILSWAIAVSGQKDVPSAEIATAQRELAGWPGAATLRANSEKALYREDPPAAQVLSAFGSSRPETAEGTVV
LGKALASSGKTDESQRLIRQLWVSEGMDKDMEDRVLAEFPAYLTPADHKARMDYLMYRSRIGQAKRFGDLGKAQSLYNAW
AAVLQRSANATALLGKVDASWRNDPTYLFARIENLRNQQKYPEAAVLLRQAPKETAKLVNPGEWWNERRIIARGVADLGD
FAEAYRIVANHSATSSVDIADAEFHAGWYALRALRDPTTAARHFNTLLQTSNRPLSASRAYYWLGRTAEAGGAGNARDFF
AKAAAHPGTFYGQLAAARIGTKTLNVSYPNPSNGDRERFAGREAVRAIDRLEAAGYGWRADSLYRSLAEQLDSPGELAIL
AARAEGNGNHQVSLQIGKTAFSRGIDAAALAYPLGVIPASANIAGSGKALAYAIARQESAFNPAAVSAANARGLLQLLPG
TAKGVAGRHGLPYTQAMLTSDAGYNATLGAHYLGEQIDSFGGSYILTFVAYNAGPKRVPEWISRYGDPRGKPIDEVVDWI
ERIPFPETRNYVQRVMENYQVYKTRLGQQADIVDDLRHGRAG

Sequences:

>Translated_682_residues
MAAGLAATVWNALAGPLPEGAAPLPYVKPNAPAVPAFMPASPEITGAIPRLNRPDDTSQLKAGLDALSSRDAARAIALRN
TMPSGSLDRHILSWAIAVSGQKDVPSAEIATAQRELAGWPGAATLRANSEKALYREDPPAAQVLSAFGSSRPETAEGTVV
LGKALASSGKTDESQRLIRQLWVSEGMDKDMEDRVLAEFPAYLTPADHKARMDYLMYRSRIGQAKRFGDLGKAQSLYNAW
AAVLQRSANATALLGKVDASWRNDPTYLFARIENLRNQQKYPEAAVLLRQAPKETAKLVNPGEWWNERRIIARGVADLGD
FAEAYRIVANHSATSSVDIADAEFHAGWYALRALRDPTTAARHFNTLLQTSNRPLSASRAYYWLGRTAEAGGAGNARDFF
AKAAAHPGTFYGQLAAARIGTKTLNVSYPNPSNGDRERFAGREAVRAIDRLEAAGYGWRADSLYRSLAEQLDSPGELAIL
AARAEGNGNHQVSLQIGKTAFSRGIDAAALAYPLGVIPASANIAGSGKALAYAIARQESAFNPAAVSAANARGLLQLLPG
TAKGVAGRHGLPYTQAMLTSDAGYNATLGAHYLGEQIDSFGGSYILTFVAYNAGPKRVPEWISRYGDPRGKPIDEVVDWI
ERIPFPETRNYVQRVMENYQVYKTRLGQQADIVDDLRHGRAG
>Mature_681_residues
AAGLAATVWNALAGPLPEGAAPLPYVKPNAPAVPAFMPASPEITGAIPRLNRPDDTSQLKAGLDALSSRDAARAIALRNT
MPSGSLDRHILSWAIAVSGQKDVPSAEIATAQRELAGWPGAATLRANSEKALYREDPPAAQVLSAFGSSRPETAEGTVVL
GKALASSGKTDESQRLIRQLWVSEGMDKDMEDRVLAEFPAYLTPADHKARMDYLMYRSRIGQAKRFGDLGKAQSLYNAWA
AVLQRSANATALLGKVDASWRNDPTYLFARIENLRNQQKYPEAAVLLRQAPKETAKLVNPGEWWNERRIIARGVADLGDF
AEAYRIVANHSATSSVDIADAEFHAGWYALRALRDPTTAARHFNTLLQTSNRPLSASRAYYWLGRTAEAGGAGNARDFFA
KAAAHPGTFYGQLAAARIGTKTLNVSYPNPSNGDRERFAGREAVRAIDRLEAAGYGWRADSLYRSLAEQLDSPGELAILA
ARAEGNGNHQVSLQIGKTAFSRGIDAAALAYPLGVIPASANIAGSGKALAYAIARQESAFNPAAVSAANARGLLQLLPGT
AKGVAGRHGLPYTQAMLTSDAGYNATLGAHYLGEQIDSFGGSYILTFVAYNAGPKRVPEWISRYGDPRGKPIDEVVDWIE
RIPFPETRNYVQRVMENYQVYKTRLGQQADIVDDLRHGRAG

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=570, Percent_Identity=27.719298245614, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 73468; Mature: 73337

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAGLAATVWNALAGPLPEGAAPLPYVKPNAPAVPAFMPASPEITGAIPRLNRPDDTSQL
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
KAGLDALSSRDAARAIALRNTMPSGSLDRHILSWAIAVSGQKDVPSAEIATAQRELAGWP
HHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHEECCCCCCCCHHHHHHHHHHCCCC
GAATLRANSEKALYREDPPAAQVLSAFGSSRPETAEGTVVLGKALASSGKTDESQRLIRQ
CCEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEHHHHHCCCCCCHHHHHHHH
LWVSEGMDKDMEDRVLAEFPAYLTPADHKARMDYLMYRSRIGQAKRFGDLGKAQSLYNAW
HHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
AAVLQRSANATALLGKVDASWRNDPTYLFARIENLRNQQKYPEAAVLLRQAPKETAKLVN
HHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHCCC
PGEWWNERRIIARGVADLGDFAEAYRIVANHSATSSVDIADAEFHAGWYALRALRDPTTA
CCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHH
ARHFNTLLQTSNRPLSASRAYYWLGRTAEAGGAGNARDFFAKAAAHPGTFYGQLAAARIG
HHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHC
TKTLNVSYPNPSNGDRERFAGREAVRAIDRLEAAGYGWRADSLYRSLAEQLDSPGELAIL
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEE
AARAEGNGNHQVSLQIGKTAFSRGIDAAALAYPLGVIPASANIAGSGKALAYAIARQESA
EEECCCCCCEEEEEEECHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCC
FNPAAVSAANARGLLQLLPGTAKGVAGRHGLPYTQAMLTSDAGYNATLGAHYLGEQIDSF
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHC
GGSYILTFVAYNAGPKRVPEWISRYGDPRGKPIDEVVDWIERIPFPETRNYVQRVMENYQ
CCCEEEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VYKTRLGQQADIVDDLRHGRAG
HHHHHCCCCCHHHHHHHCCCCC
>Mature Secondary Structure 
AAGLAATVWNALAGPLPEGAAPLPYVKPNAPAVPAFMPASPEITGAIPRLNRPDDTSQL
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
KAGLDALSSRDAARAIALRNTMPSGSLDRHILSWAIAVSGQKDVPSAEIATAQRELAGWP
HHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHEECCCCCCCCHHHHHHHHHHCCCC
GAATLRANSEKALYREDPPAAQVLSAFGSSRPETAEGTVVLGKALASSGKTDESQRLIRQ
CCEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEHHHHHCCCCCCHHHHHHHH
LWVSEGMDKDMEDRVLAEFPAYLTPADHKARMDYLMYRSRIGQAKRFGDLGKAQSLYNAW
HHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
AAVLQRSANATALLGKVDASWRNDPTYLFARIENLRNQQKYPEAAVLLRQAPKETAKLVN
HHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHCCC
PGEWWNERRIIARGVADLGDFAEAYRIVANHSATSSVDIADAEFHAGWYALRALRDPTTA
CCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHH
ARHFNTLLQTSNRPLSASRAYYWLGRTAEAGGAGNARDFFAKAAAHPGTFYGQLAAARIG
HHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHC
TKTLNVSYPNPSNGDRERFAGREAVRAIDRLEAAGYGWRADSLYRSLAEQLDSPGELAIL
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEE
AARAEGNGNHQVSLQIGKTAFSRGIDAAALAYPLGVIPASANIAGSGKALAYAIARQESA
EEECCCCCCEEEEEEECHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCC
FNPAAVSAANARGLLQLLPGTAKGVAGRHGLPYTQAMLTSDAGYNATLGAHYLGEQIDSF
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHC
GGSYILTFVAYNAGPKRVPEWISRYGDPRGKPIDEVVDWIERIPFPETRNYVQRVMENYQ
CCCEEEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VYKTRLGQQADIVDDLRHGRAG
HHHHHCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]