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The map label for this gene is iciA [C]

Identifier: 159184533

GI number: 159184533

Start: 915880

End: 916785

Strand: Direct

Name: iciA [C]

Synonym: Atu0928

Alternate gene names: 159184533

Gene position: 915880-916785 (Clockwise)

Preceding gene: 15888264

Following gene: 159184535

Centisome position: 32.23

GC content: 64.02

Gene sequence:

>906_bases
ATGCTCGACTATCCTTCCATGCGCGCCGTGGCGCTCGTTGCCCGGACCGGCAGTTTCGAAAAGGCGGCGCAGGTGCTGTG
TGTCACGCCATCAGCCGTTTCCCAGCGTATCAAGCAGCTGGAGGAGCGGCTGGGCGTTGTGCTGATCGTGCGCGGCAACC
CTTGCGTGGCCACGGAAAAGGGCGAATGGCTCTGCCGGCACATGGATCATGTCGGCATGCTGGAAAGTGAATTGTTCCGC
CAGCTTCCAGCGCTCACGGAGGCAGGCGATGCGCAGGAGCGGGTGACGCTCAACATCGCCACCAATGCCGACAGCCTCGG
CACCTGGTTTCTGGACGCGGTGTCGAAATTCACTGGCGGCAGCGATTATCTCGTCAATATCGCCGTCGACGATCAGGATC
ACACGGTGGAATGGCTGCGCGGCGGCAGGGTGCTCGCCGCCGTCACCGCCCACGACAAACCGGTGCAGGGTTGCCGGGTA
ACGCCGCTTGGCGTGCTGCGTTATCATGCCACCGCCAGCCCGGATTTCATGGCCCGCCATTTTGCCGATGGCGTCACGCC
CGCCGCCCTTGCCCGCGCGCCGGGATTGACCTTCAACCAGAAGGACAGGCTGCAGGCGAGCTGGATCAGGACAGCGCTGG
GGGAGGATGTCTCCTATCCCACCCACTGGCTGCCTTCGACCGATGGTTTTGTGAAAGCGAGCCTTGCCGGCATGGGGTGG
GGGCTCAATCCGGTGCAGCTGGTGGCGGAGCATCTGGCTGCTGGCCGCCTGGTTGAGCTGATGCCGGGCACGCCGCTTGA
TATTCCGCTTTATTGGCAGGTGAACCGGCTTGCGGCGGAGCGGTTGGCCGGGTTGACGGCAAATATGGTGGGGACGGCGA
GGGTGGTGTTGATGCCGGTAGGGTGA

Upstream 100 bases:

>100_bases
ATTCCTGTGACAAGCACAGGAATGAGGAGAGGCGAGCTCCTCACCAATGGTCTGTAGCGGCGCACCAGTCGCATTAATTC
CAACGTCCTCAGGTGCCATA

Downstream 100 bases:

>100_bases
GGTGGTTCGGGTCGTGCGGCGGTGGGCGATGTGGTGAGCGCTGGAACGTGTGGTTCACCCCCCTCTGTCCTGCCGGACAT
CTCCCCCACAAGGGGGGAGA

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MLDYPSMRAVALVARTGSFEKAAQVLCVTPSAVSQRIKQLEERLGVVLIVRGNPCVATEKGEWLCRHMDHVGMLESELFR
QLPALTEAGDAQERVTLNIATNADSLGTWFLDAVSKFTGGSDYLVNIAVDDQDHTVEWLRGGRVLAAVTAHDKPVQGCRV
TPLGVLRYHATASPDFMARHFADGVTPAALARAPGLTFNQKDRLQASWIRTALGEDVSYPTHWLPSTDGFVKASLAGMGW
GLNPVQLVAEHLAAGRLVELMPGTPLDIPLYWQVNRLAAERLAGLTANMVGTARVVLMPVG

Sequences:

>Translated_301_residues
MLDYPSMRAVALVARTGSFEKAAQVLCVTPSAVSQRIKQLEERLGVVLIVRGNPCVATEKGEWLCRHMDHVGMLESELFR
QLPALTEAGDAQERVTLNIATNADSLGTWFLDAVSKFTGGSDYLVNIAVDDQDHTVEWLRGGRVLAAVTAHDKPVQGCRV
TPLGVLRYHATASPDFMARHFADGVTPAALARAPGLTFNQKDRLQASWIRTALGEDVSYPTHWLPSTDGFVKASLAGMGW
GLNPVQLVAEHLAAGRLVELMPGTPLDIPLYWQVNRLAAERLAGLTANMVGTARVVLMPVG
>Mature_301_residues
MLDYPSMRAVALVARTGSFEKAAQVLCVTPSAVSQRIKQLEERLGVVLIVRGNPCVATEKGEWLCRHMDHVGMLESELFR
QLPALTEAGDAQERVTLNIATNADSLGTWFLDAVSKFTGGSDYLVNIAVDDQDHTVEWLRGGRVLAAVTAHDKPVQGCRV
TPLGVLRYHATASPDFMARHFADGVTPAALARAPGLTFNQKDRLQASWIRTALGEDVSYPTHWLPSTDGFVKASLAGMGW
GLNPVQLVAEHLAAGRLVELMPGTPLDIPLYWQVNRLAAERLAGLTANMVGTARVVLMPVG

Specific function: Specific Inhibitor Of Chromosomal Initiation Of Replication In VItro. Binds The Three 13-Mers In The Origin (Oric) To Block Initiation Of Replication. [C]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789283, Length=282, Percent_Identity=36.5248226950355, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1786713, Length=283, Percent_Identity=26.1484098939929, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32588; Mature: 32588

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDYPSMRAVALVARTGSFEKAAQVLCVTPSAVSQRIKQLEERLGVVLIVRGNPCVATEK
CCCCCCCCEEEEEECCCCHHHHCEEEEECHHHHHHHHHHHHHHCCEEEEECCCCEEECCC
GEWLCRHMDHVGMLESELFRQLPALTEAGDAQERVTLNIATNADSLGTWFLDAVSKFTGG
CHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCC
SDYLVNIAVDDQDHTVEWLRGGRVLAAVTAHDKPVQGCRVTPLGVLRYHATASPDFMARH
CCEEEEEEECCCCCHHHHHCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHHH
FADGVTPAALARAPGLTFNQKDRLQASWIRTALGEDVSYPTHWLPSTDGFVKASLAGMGW
HHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHHCCC
GLNPVQLVAEHLAAGRLVELMPGTPLDIPLYWQVNRLAAERLAGLTANMVGTARVVLMPV
CCCHHHHHHHHHHCCCEEEECCCCCCCEEEEEEHHHHHHHHHHCCHHHCCCCEEEEEEEC
G
C
>Mature Secondary Structure
MLDYPSMRAVALVARTGSFEKAAQVLCVTPSAVSQRIKQLEERLGVVLIVRGNPCVATEK
CCCCCCCCEEEEEECCCCHHHHCEEEEECHHHHHHHHHHHHHHCCEEEEECCCCEEECCC
GEWLCRHMDHVGMLESELFRQLPALTEAGDAQERVTLNIATNADSLGTWFLDAVSKFTGG
CHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCC
SDYLVNIAVDDQDHTVEWLRGGRVLAAVTAHDKPVQGCRVTPLGVLRYHATASPDFMARH
CCEEEEEEECCCCCHHHHHCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHHH
FADGVTPAALARAPGLTFNQKDRLQASWIRTALGEDVSYPTHWLPSTDGFVKASLAGMGW
HHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHHCCC
GLNPVQLVAEHLAAGRLVELMPGTPLDIPLYWQVNRLAAERLAGLTANMVGTARVVLMPV
CCCHHHHHHHHHHCCCEEEECCCCCCCEEEEEEHHHHHHHHHHCCHHHCCCCEEEEEEEC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]