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The map label for this gene is cysH

Identifier: 159184476

GI number: 159184476

Start: 816493

End: 817254

Strand: Reverse

Name: cysH

Synonym: Atu0818

Alternate gene names: 159184476

Gene position: 817254-816493 (Counterclockwise)

Preceding gene: 159184481

Following gene: 159184475

Centisome position: 28.76

GC content: 61.02

Gene sequence:

>762_bases
ATGACCATCAATTCAACAAATGCTTCTGCCGATACCGCATCCCTCGACGCCACGCTTGCCGGGCTCGATCTTGCCGGACG
TCTTTCTTTCGTTGCCGGCCTCGGCGGGCGCGCGGTCTTCACCACCAGCCTCGGCATCGAGGATCAGGTCATCACCGCCG
CCATCGGTACGCATCGTCTGCCGATTGACGTCGTGACGCTGGAAACCGGGCGGCTGTTCAAGGAGACAGTCGATCTCATC
GATGAAACCGAAGAACGTTTCGGCATCGAAATCCGCCGCTTCCGTCCCGAACAAGACGATATCGACGCCTATGCAGCGAA
GTATGGTCTCAACGGCTTTTACGAGAGCGTCGAAGCCCGCCACGCCTGCTGTCATGTCAGAAAGCTGATCCCGCTTGGCA
AGGCACTGGAGGGTGCAGCTTTCTGGATCACCGGGCTCCGTCGCGGCCAGTCCGGCAATCGCGCCGCGACCCCATTCGCC
GAGTTCGACGCTGAGCGTAATCTTATCAAGATCAATGCTTTGGCCGACTGGGACATCGAACAGATCAGGGCCTATGTCGC
CGAGGAAAACATCCCCGTCAACCCGTTGCATCAGCGCGGTTACCCCTCCATCGGCTGCGAGCCGTGTACGCGCGCCATCA
AGCCCGGCGAGCCTGAGCGCGCCGGACGATGGTGGTGGGAGAACGACGAGAAGCGCGAATGCGGTCTTCACGTCGCCGGT
GCGGAGCAGACACCCCCCGTTTCCGCCATCCCGCAACGATAA

Upstream 100 bases:

>100_bases
GAGGCATTTTTGCTCTGAAGTGCAGCGCACAAAGACTGTCATTTCTGTTCGCATAGCCGGATTTCAACAATAATCGGCCA
TGGAAATCCCGGACGTGACA

Downstream 100 bases:

>100_bases
TCATTCAGTTCATCTCCCGGAGTTCACAATGTCCAACGCAGTCCACGAGACGGACAGCAAGAATACTGTCGCATCCAAGC
CGCCGCTCGATCCGCATCTG

Product: phosphoadenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MTINSTNASADTASLDATLAGLDLAGRLSFVAGLGGRAVFTTSLGIEDQVITAAIGTHRLPIDVVTLETGRLFKETVDLI
DETEERFGIEIRRFRPEQDDIDAYAAKYGLNGFYESVEARHACCHVRKLIPLGKALEGAAFWITGLRRGQSGNRAATPFA
EFDAERNLIKINALADWDIEQIRAYVAEENIPVNPLHQRGYPSIGCEPCTRAIKPGEPERAGRWWWENDEKRECGLHVAG
AEQTPPVSAIPQR

Sequences:

>Translated_253_residues
MTINSTNASADTASLDATLAGLDLAGRLSFVAGLGGRAVFTTSLGIEDQVITAAIGTHRLPIDVVTLETGRLFKETVDLI
DETEERFGIEIRRFRPEQDDIDAYAAKYGLNGFYESVEARHACCHVRKLIPLGKALEGAAFWITGLRRGQSGNRAATPFA
EFDAERNLIKINALADWDIEQIRAYVAEENIPVNPLHQRGYPSIGCEPCTRAIKPGEPERAGRWWWENDEKRECGLHVAG
AEQTPPVSAIPQR
>Mature_252_residues
TINSTNASADTASLDATLAGLDLAGRLSFVAGLGGRAVFTTSLGIEDQVITAAIGTHRLPIDVVTLETGRLFKETVDLID
ETEERFGIEIRRFRPEQDDIDAYAAKYGLNGFYESVEARHACCHVRKLIPLGKALEGAAFWITGLRRGQSGNRAATPFAE
FDAERNLIKINALADWDIEQIRAYVAEENIPVNPLHQRGYPSIGCEPCTRAIKPGEPERAGRWWWENDEKRECGLHVAGA
EQTPPVSAIPQR

Specific function: Reduction of activated sulfate into sulfite

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily

Homologues:

Organism=Escherichia coli, GI1789121, Length=228, Percent_Identity=32.4561403508772, Blast_Score=106, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6325425, Length=213, Percent_Identity=30.0469483568075, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSH_AGRT5 (Q8UH67)

Other databases:

- EMBL:   AE007869
- PIR:   AB2677
- PIR:   H97458
- RefSeq:   NP_353840.2
- ProteinModelPortal:   Q8UH67
- SMR:   Q8UH67
- STRING:   Q8UH67
- GeneID:   1132856
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0818
- eggNOG:   COG0175
- HOGENOM:   HBG758022
- OMA:   CTRAIKP
- PhylomeDB:   Q8UH67
- ProtClustDB:   CLSK886291
- BioCyc:   ATUM176299-1:ATU0818-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00063
- InterPro:   IPR011798
- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR02055

Pfam domain/function: PF01507 PAPS_reduct

EC number: =1.8.4.8

Molecular weight: Translated: 27830; Mature: 27698

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTINSTNASADTASLDATLAGLDLAGRLSFVAGLGGRAVFTTSLGIEDQVITAAIGTHRL
CCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCCCCHHEEEECCCCCC
PIDVVTLETGRLFKETVDLIDETEERFGIEIRRFRPEQDDIDAYAAKYGLNGFYESVEAR
CEEEEEECCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHH
HACCHVRKLIPLGKALEGAAFWITGLRRGQSGNRAATPFAEFDAERNLIKINALADWDIE
HHHHHHHHHHHHCCHHCCHHHEEHHHHCCCCCCCCCCCHHHHCCCCCEEEEEEECCCCHH
QIRAYVAEENIPVNPLHQRGYPSIGCEPCTRAIKPGEPERAGRWWWENDEKRECGLHVAG
HHHHHHHCCCCCCCCHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHCCEEEEC
AEQTPPVSAIPQR
CCCCCCCCCCCCC
>Mature Secondary Structure 
TINSTNASADTASLDATLAGLDLAGRLSFVAGLGGRAVFTTSLGIEDQVITAAIGTHRL
CCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCCCCHHEEEECCCCCC
PIDVVTLETGRLFKETVDLIDETEERFGIEIRRFRPEQDDIDAYAAKYGLNGFYESVEAR
CEEEEEECCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHH
HACCHVRKLIPLGKALEGAAFWITGLRRGQSGNRAATPFAEFDAERNLIKINALADWDIE
HHHHHHHHHHHHCCHHCCHHHEEHHHHCCCCCCCCCCCHHHHCCCCCEEEEEEECCCCHH
QIRAYVAEENIPVNPLHQRGYPSIGCEPCTRAIKPGEPERAGRWWWENDEKRECGLHVAG
HHHHHHHCCCCCCCCHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHCCEEEEC
AEQTPPVSAIPQR
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194