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The map label for this gene is kshB [H]

Identifier: 159184441

GI number: 159184441

Start: 727575

End: 728507

Strand: Direct

Name: kshB [H]

Synonym: Atu0727

Alternate gene names: 159184441

Gene position: 727575-728507 (Clockwise)

Preceding gene: 159184440

Following gene: 15888072

Centisome position: 25.6

GC content: 61.52

Gene sequence:

>933_bases
CTGCCGGGCCAGTTCGTCACGCTGGAATTGCCCGTGGGTTCGGAGCCGATTTACCGCACCTACACACTGTCCTCCAGCCC
GTCGCGGCCCTATGCGCTGTCGGTCACGGTCAAGGCGCAGGCCACCAGCATCGGCACGCGCTGGATGTTCGATAATCTGA
AGCCCGGCATGAAAGTCCGGGCGCTCGGACCGCTCGGCGACTTCTCCTACGTCAAACATCCCGGCGACAAATATCTGTTC
ATCTCGGCAGGCTCCGGCGTCACGCCGATGATGTCGATGGTGCGCGACATGAGCGACCGCGCGCCGCAGAGCGACATCAC
CTTCATCAACTGTTCACGCACCCCCGGCGATATCGTCTTCCGTCACGAGCTGGAATATCTCGCCCGTTTCATGCCTAACC
TGTCGCTCGGCTTCATTGTCGAGAAATGCGGTCGTACCGATCTCTGGTCCGGACTGAGGGGCATGGTCGACAAGGCCAAG
ATCGCGCTGCTCGCGCATGATTTCATGGATCGCACCGTCTTCTGTTGCGGTCCGGAGCCGTTCATGGCCGCCGTCCGCTC
CATGCTCGACGCATCCGGCTTCGATATGAGCCGCTATCATCAGGAAAGCTTTGCGCCGGCCGCTCCGGTCAGCGTGGGTG
AAACAGTTCTCACCGGTGCCGACGGCGAAGCGTTGTCCATGGTGGGCTTCACTCTTTCTGGAAAAGAGCTGCCCTGCCAG
CCAGGCCAGACGGTTCTGATGACGGCGCGTGCTGCCGGCGTCCGCATCGGCGCCGCCTGCGAATCCGGTATCTGCGGCAC
CTGCCGGGTGCTGAAGCTTTCCGGCGAGGTGGAGATGAACCACAATGGCGGCATTCTCGATGAGGAAATCGAGGAAGGCT
ATATTCTTGCCTGCTGCTCACGGCCATTGACCGACATCAAGGTGGAGGCGTGA

Upstream 100 bases:

>100_bases
CGACAAGCTCCAGCTGCTGGAATGCATTTCAGTGACGCCCGAGACATCGGACGTGATGACCTTCCTGTTCCGCTCCGAGG
ACCAGAACTGGTTCCGTTAT

Downstream 100 bases:

>100_bases
CGTCCAACGGCGTCACGTTCTAATTTTCCTTCGCCGAATAGCTGTCTGGAACTAAATTTTAGCAAACTCGTTTGCACGTC
ATCGAATTCTTTTCGGGCGA

Product: ferredoxin I

Products: NA

Alternate protein names: Rieske-type oxygenase; RO [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MPGQFVTLELPVGSEPIYRTYTLSSSPSRPYALSVTVKAQATSIGTRWMFDNLKPGMKVRALGPLGDFSYVKHPGDKYLF
ISAGSGVTPMMSMVRDMSDRAPQSDITFINCSRTPGDIVFRHELEYLARFMPNLSLGFIVEKCGRTDLWSGLRGMVDKAK
IALLAHDFMDRTVFCCGPEPFMAAVRSMLDASGFDMSRYHQESFAPAAPVSVGETVLTGADGEALSMVGFTLSGKELPCQ
PGQTVLMTARAAGVRIGAACESGICGTCRVLKLSGEVEMNHNGGILDEEIEEGYILACCSRPLTDIKVEA

Sequences:

>Translated_310_residues
MPGQFVTLELPVGSEPIYRTYTLSSSPSRPYALSVTVKAQATSIGTRWMFDNLKPGMKVRALGPLGDFSYVKHPGDKYLF
ISAGSGVTPMMSMVRDMSDRAPQSDITFINCSRTPGDIVFRHELEYLARFMPNLSLGFIVEKCGRTDLWSGLRGMVDKAK
IALLAHDFMDRTVFCCGPEPFMAAVRSMLDASGFDMSRYHQESFAPAAPVSVGETVLTGADGEALSMVGFTLSGKELPCQ
PGQTVLMTARAAGVRIGAACESGICGTCRVLKLSGEVEMNHNGGILDEEIEEGYILACCSRPLTDIKVEA
>Mature_309_residues
PGQFVTLELPVGSEPIYRTYTLSSSPSRPYALSVTVKAQATSIGTRWMFDNLKPGMKVRALGPLGDFSYVKHPGDKYLFI
SAGSGVTPMMSMVRDMSDRAPQSDITFINCSRTPGDIVFRHELEYLARFMPNLSLGFIVEKCGRTDLWSGLRGMVDKAKI
ALLAHDFMDRTVFCCGPEPFMAAVRSMLDASGFDMSRYHQESFAPAAPVSVGETVLTGADGEALSMVGFTLSGKELPCQP
GQTVLMTARAAGVRIGAACESGICGTCRVLKLSGEVEMNHNGGILDEEIEEGYILACCSRPLTDIKVEA

Specific function: Catalyzes the opening of ring B of 1,4-androstadiene- 3,17,-dione (ADD) and 4-androstene-3,17-dione (ADD) with concomitant aromatization of ring A. The ring B is subsequently hydroxylated to yield a catechol and then subject to meta-cleavage [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1787098, Length=307, Percent_Identity=30.2931596091205, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1787658, Length=317, Percent_Identity=28.391167192429, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1788104, Length=304, Percent_Identity=26.6447368421053, Blast_Score=86, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323552, Length=179, Percent_Identity=24.5810055865922, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 33537; Mature: 33406

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
8.4 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
8.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGQFVTLELPVGSEPIYRTYTLSSSPSRPYALSVTVKAQATSIGTRWMFDNLKPGMKVR
CCCCEEEEEECCCCCCCEEEEEECCCCCCCEEEEEEEEEEECCCCCEEEECCCCCCCEEE
ALGPLGDFSYVKHPGDKYLFISAGSGVTPMMSMVRDMSDRAPQSDITFINCSRTPGDIVF
EECCCCCCHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEE
RHELEYLARFMPNLSLGFIVEKCGRTDLWSGLRGMVDKAKIALLAHDFMDRTVFCCGPEP
HHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCH
FMAAVRSMLDASGFDMSRYHQESFAPAAPVSVGETVLTGADGEALSMVGFTLSGKELPCQ
HHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCEEEECCCCCEEEEEEEEECCCCCCCC
PGQTVLMTARAAGVRIGAACESGICGTCRVLKLSGEVEMNHNGGILDEEIEEGYILACCS
CCCEEEEEEECCCEEEECCCCCCCCCEEEEEEECCEEEECCCCCCCCHHHCCCEEEEECC
RPLTDIKVEA
CCCCCEEECC
>Mature Secondary Structure 
PGQFVTLELPVGSEPIYRTYTLSSSPSRPYALSVTVKAQATSIGTRWMFDNLKPGMKVR
CCCEEEEEECCCCCCCEEEEEECCCCCCCEEEEEEEEEEECCCCCEEEECCCCCCCEEE
ALGPLGDFSYVKHPGDKYLFISAGSGVTPMMSMVRDMSDRAPQSDITFINCSRTPGDIVF
EECCCCCCHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEE
RHELEYLARFMPNLSLGFIVEKCGRTDLWSGLRGMVDKAKIALLAHDFMDRTVFCCGPEP
HHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCH
FMAAVRSMLDASGFDMSRYHQESFAPAAPVSVGETVLTGADGEALSMVGFTLSGKELPCQ
HHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCEEEECCCCCEEEEEEEEECCCCCCCC
PGQTVLMTARAAGVRIGAACESGICGTCRVLKLSGEVEMNHNGGILDEEIEEGYILACCS
CCCEEEEEEECCCEEEECCCCCCCCCEEEEEEECCEEEECCCCCCCCHHHCCCEEEEECC
RPLTDIKVEA
CCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA