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The map label for this gene is glcD [H]

Identifier: 159184412

GI number: 159184412

Start: 660204

End: 661637

Strand: Direct

Name: glcD [H]

Synonym: Atu0665

Alternate gene names: 159184412

Gene position: 660204-661637 (Clockwise)

Preceding gene: 15888005

Following gene: 159184413

Centisome position: 23.23

GC content: 60.32

Gene sequence:

>1434_bases
GTGACTCAGCCGATCAAGTTTCTGGAGCCGCGCGCCAAGGTCGTCGCGAGCCGCGACCGCATCATCGCCGACCTTAAGGA
TATCCTGCCGGAGGACTGTCTTGTGCACGAGCCGCGCGAGCTCGTGCCTTTCGAAACCGATGCCTTCGTCTCCTATCGCC
GCCTGCCGCTGGCCGTAGCACTGCCGAAAACGACGGAAGAAGTGGCGGCAGTGATGAGATATTGCCACCGTTACGGCATT
CCCGTCGTGCCGCGCGGCGCCGGCACCTCGCTGTCCGGTGGCGCTATTCCGCAAGAGGATGCTGTCGTGATCGGCCTCTC
GAAGATGAGCGCGATCCTCGATCTTGATTTTTACAATCGCACGGCGCGGGTGCAGGCGGGTGTCACCAATCTTTCCATTT
CCGATGCGGCCGGGGCTGAGGGTTTTTTTTATGCGCCCGATCCCAGCTCGCAGCTCGCCTGCACCATTGGCGGCAATATC
GGCATGAATTCCGGTGGTGCGCATTGTCTGAAATATGGTGTCACCACCAATAATCTGCTTGGCGTGAAAATGGTGCTGGT
GGATGGCACGGTGCTGGAACTGGGCGGCAATCACCTCGATGCCGCAGGCTACGATCTTCTCGCTCTCGTCTGCGGTTCGG
AAGGCCAGCTCGGCATCGTCACGGAAGCGACGGTGCGGCTGATCGCCAGGCCGGAAGGGGCGCGGCCCGTGCTGTTCGGT
TTCGAGACCTCTGAAGAAGCGGGCTCCTGCGTAGCCGATATCATCGGCGCCGGCATCATCCCTGTTGCAATCGAATTCAT
GGACAAACCGGCCATCGAGATTTGCGAGGCCTTCGCCAAGGCGGGCTATCCACTTGACGTCGGTGCGCTTCTGATCATCG
AGGTCGAAGGCTCCGAGGCGGAAATGGACGCCATGCTGGCCGATATCGTCACCATTGCCAGAAAACACGGTGTGAAGACG
GTGAAAGAATGTCAGTCGGCTATGGAGGCTGCGGCCATCTGGAAGGGCCGCAAATCCGCCTTCGGCGCAACCGGCCGTAT
CGCGGATTACATCTGCATGGATGGCACGGTGCCGCTTTCCCAGCTTTCCTATGTGCTGAAAAAGACCAGCGAAATTACCG
ACCGTCTCGGCCTGCGCGTTGCCAATGTCTTCCATGCCGGCGATGGCAACATGCACCCGCTCATCCTGTTCAACGCCAAT
GACCCTGACGATGCGGCCCGCGCGGAAGAGGCTGGCAATGAAATATTGAAGCTCTGCGTCGATGCCGGCGGCTGTCTCAC
CGGTGAACACGGTGTAGGCATCGAAAAACGCGACCTGATGCGCCACCAATATGGCGAGGCCGATCTTGCCCAGCAGATGG
CGGTGCGGGCGGCCTTTGACGAGGGCTGGCTGATGAACCCTTCCAAGGTGTTTCCGCTGGAGGGGAGAGGGTGA

Upstream 100 bases:

>100_bases
ATTATTTCCAGAATGGAAAAAATCCATAACATTTGATTGCGCCGTTTCAAAGTGGTAAAGAAAGAATACCAGTTGGAGGA
ACGTGCATGGAGGAGGGGCG

Downstream 100 bases:

>100_bases
TGGGAGTGGGATTTGTGACGCTTGCGGGTGTGGCTTACCCCCCTCTGCCCTGCCGGGCATCTCCCCCACAAGGAGGGAGA
TCGGCAAGATGCGCTCGCGT

Product: glycolate oxidase subunit

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MTQPIKFLEPRAKVVASRDRIIADLKDILPEDCLVHEPRELVPFETDAFVSYRRLPLAVALPKTTEEVAAVMRYCHRYGI
PVVPRGAGTSLSGGAIPQEDAVVIGLSKMSAILDLDFYNRTARVQAGVTNLSISDAAGAEGFFYAPDPSSQLACTIGGNI
GMNSGGAHCLKYGVTTNNLLGVKMVLVDGTVLELGGNHLDAAGYDLLALVCGSEGQLGIVTEATVRLIARPEGARPVLFG
FETSEEAGSCVADIIGAGIIPVAIEFMDKPAIEICEAFAKAGYPLDVGALLIIEVEGSEAEMDAMLADIVTIARKHGVKT
VKECQSAMEAAAIWKGRKSAFGATGRIADYICMDGTVPLSQLSYVLKKTSEITDRLGLRVANVFHAGDGNMHPLILFNAN
DPDDAARAEEAGNEILKLCVDAGGCLTGEHGVGIEKRDLMRHQYGEADLAQQMAVRAAFDEGWLMNPSKVFPLEGRG

Sequences:

>Translated_477_residues
MTQPIKFLEPRAKVVASRDRIIADLKDILPEDCLVHEPRELVPFETDAFVSYRRLPLAVALPKTTEEVAAVMRYCHRYGI
PVVPRGAGTSLSGGAIPQEDAVVIGLSKMSAILDLDFYNRTARVQAGVTNLSISDAAGAEGFFYAPDPSSQLACTIGGNI
GMNSGGAHCLKYGVTTNNLLGVKMVLVDGTVLELGGNHLDAAGYDLLALVCGSEGQLGIVTEATVRLIARPEGARPVLFG
FETSEEAGSCVADIIGAGIIPVAIEFMDKPAIEICEAFAKAGYPLDVGALLIIEVEGSEAEMDAMLADIVTIARKHGVKT
VKECQSAMEAAAIWKGRKSAFGATGRIADYICMDGTVPLSQLSYVLKKTSEITDRLGLRVANVFHAGDGNMHPLILFNAN
DPDDAARAEEAGNEILKLCVDAGGCLTGEHGVGIEKRDLMRHQYGEADLAQQMAVRAAFDEGWLMNPSKVFPLEGRG
>Mature_476_residues
TQPIKFLEPRAKVVASRDRIIADLKDILPEDCLVHEPRELVPFETDAFVSYRRLPLAVALPKTTEEVAAVMRYCHRYGIP
VVPRGAGTSLSGGAIPQEDAVVIGLSKMSAILDLDFYNRTARVQAGVTNLSISDAAGAEGFFYAPDPSSQLACTIGGNIG
MNSGGAHCLKYGVTTNNLLGVKMVLVDGTVLELGGNHLDAAGYDLLALVCGSEGQLGIVTEATVRLIARPEGARPVLFGF
ETSEEAGSCVADIIGAGIIPVAIEFMDKPAIEICEAFAKAGYPLDVGALLIIEVEGSEAEMDAMLADIVTIARKHGVKTV
KECQSAMEAAAIWKGRKSAFGATGRIADYICMDGTVPLSQLSYVLKKTSEITDRLGLRVANVFHAGDGNMHPLILFNAND
PDDAARAEEAGNEILKLCVDAGGCLTGEHGVGIEKRDLMRHQYGEADLAQQMAVRAAFDEGWLMNPSKVFPLEGRG

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=423, Percent_Identity=29.5508274231678, Blast_Score=200, Evalue=3e-51,
Organism=Homo sapiens, GI37595754, Length=446, Percent_Identity=28.0269058295964, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI119964728, Length=463, Percent_Identity=28.5097192224622, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI4501993, Length=231, Percent_Identity=27.7056277056277, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1789351, Length=456, Percent_Identity=48.0263157894737, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI1787977, Length=506, Percent_Identity=24.5059288537549, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI48994907, Length=492, Percent_Identity=23.3739837398374, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71992373, Length=430, Percent_Identity=28.8372093023256, Blast_Score=195, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI71992381, Length=430, Percent_Identity=28.8372093023256, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI71992392, Length=362, Percent_Identity=29.0055248618785, Blast_Score=165, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17534361, Length=431, Percent_Identity=27.3781902552204, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17556096, Length=464, Percent_Identity=21.9827586206897, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320027, Length=481, Percent_Identity=28.4823284823285, Blast_Score=202, Evalue=9e-53,
Organism=Saccharomyces cerevisiae, GI6320764, Length=500, Percent_Identity=26.6, Blast_Score=166, Evalue=9e-42,
Organism=Saccharomyces cerevisiae, GI6320023, Length=488, Percent_Identity=24.5901639344262, Blast_Score=146, Evalue=9e-36,
Organism=Drosophila melanogaster, GI18921117, Length=424, Percent_Identity=26.6509433962264, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24639277, Length=424, Percent_Identity=26.6509433962264, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24639275, Length=424, Percent_Identity=26.6509433962264, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24653753, Length=464, Percent_Identity=22.6293103448276, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 50720; Mature: 50589

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPIKFLEPRAKVVASRDRIIADLKDILPEDCLVHEPRELVPFETDAFVSYRRLPLAVA
CCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHCCCCCHHHHHHHHCCEEEE
LPKTTEEVAAVMRYCHRYGIPVVPRGAGTSLSGGAIPQEDAVVIGLSKMSAILDLDFYNR
CCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHEEEHHCCC
TARVQAGVTNLSISDAAGAEGFFYAPDPSSQLACTIGGNIGMNSGGAHCLKYGVTTNNLL
EEEEEECCEEEEECCCCCCCCEEECCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCE
GVKMVLVDGTVLELGGNHLDAAGYDLLALVCGSEGQLGIVTEATVRLIARPEGARPVLFG
EEEEEEECCEEEEECCCCCCCCCCEEEEEEECCCCCEEEEEHHHEEEEECCCCCCEEEEE
FETSEEAGSCVADIIGAGIIPVAIEFMDKPAIEICEAFAKAGYPLDVGALLIIEVEGSEA
ECCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCH
EMDAMLADIVTIARKHGVKTVKECQSAMEAAAIWKGRKSAFGATGRIADYICMDGTVPLS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCHHEEEEECCCCCHH
QLSYVLKKTSEITDRLGLRVANVFHAGDGNMHPLILFNANDPDDAARAEEAGNEILKLCV
HHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
DAGGCLTGEHGVGIEKRDLMRHQYGEADLAQQMAVRAAFDEGWLMNPSKVFPLEGRG
CCCCCEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCC
>Mature Secondary Structure 
TQPIKFLEPRAKVVASRDRIIADLKDILPEDCLVHEPRELVPFETDAFVSYRRLPLAVA
CCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHCCCCCHHHHHHHHCCEEEE
LPKTTEEVAAVMRYCHRYGIPVVPRGAGTSLSGGAIPQEDAVVIGLSKMSAILDLDFYNR
CCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHEEEHHCCC
TARVQAGVTNLSISDAAGAEGFFYAPDPSSQLACTIGGNIGMNSGGAHCLKYGVTTNNLL
EEEEEECCEEEEECCCCCCCCEEECCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCE
GVKMVLVDGTVLELGGNHLDAAGYDLLALVCGSEGQLGIVTEATVRLIARPEGARPVLFG
EEEEEEECCEEEEECCCCCCCCCCEEEEEEECCCCCEEEEEHHHEEEEECCCCCCEEEEE
FETSEEAGSCVADIIGAGIIPVAIEFMDKPAIEICEAFAKAGYPLDVGALLIIEVEGSEA
ECCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCH
EMDAMLADIVTIARKHGVKTVKECQSAMEAAAIWKGRKSAFGATGRIADYICMDGTVPLS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCHHEEEEECCCCCHH
QLSYVLKKTSEITDRLGLRVANVFHAGDGNMHPLILFNANDPDDAARAEEAGNEILKLCV
HHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
DAGGCLTGEHGVGIEKRDLMRHQYGEADLAQQMAVRAAFDEGWLMNPSKVFPLEGRG
CCCCCEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]