Click here to switch to the map view.

The map label for this gene is purD

Identifier: 159184405

GI number: 159184405

Start: 637485

End: 638756

Strand: Reverse

Name: purD

Synonym: Atu0647

Alternate gene names: 159184405

Gene position: 638756-637485 (Counterclockwise)

Preceding gene: 15887994

Following gene: 159185417

Centisome position: 22.48

GC content: 61.95

Gene sequence:

>1272_bases
ATGAAAGTTCTGTTGATCGGTTCCGGCGGACGCGAACATGCGCTGGCGTGGAAAATCGCCCAGTCTCCCCTCATCGACGC
GCTTTATGCCGCACCCGGCAATCCCGGCATCGCCGACCATGCGACACTCGTTTCGCTCGACGTGGAAGACCACGCCGCCG
TCATCGCTTTCGCGAAGGAAAAAGCAATCGACTTCGTCGTCATCGGCCCGGAAGCACCGCTGGTCGCGGGTTTGGCGGAC
GATCTGCGCGCCGCCGGCATCGCCACTTTCGGACCTTCGAAGGCCGCCGCCCAGCTTGAGGGTTCCAAGGGCTTCACGAA
GGACCTTTGCGCCCGTTACGATATTCCGACCGGGGCTTATGAGCGTTTCAAGGCGGCCGAACCTGCCAAGGACTATGTCC
GTGCACAGGGCGCGCCGATCGTTATCAAGGCGGATGGGCTTGCCGCCGGCAAGGGCGTGACCGTGGCCATGACCGAAGCC
GAAGCGCTTGCCGCCATAGATGACTGTTTCGACGGTGCTTTCGGCACGGCCGGCACTGAGGTGGTGGTGGAAGCCTTCCT
CGACGGCGAGGAAGCGAGCTTCTTCTGCCTTTCGGATGGTAAAACGGCGCTGGCGCTCGCCACCGCGCAGGATCACAAGC
GTGTCGGCGATGGCGATACCGGCCCGAATACCGGCGGCATGGGTGCTTACTCTCCCGCCCCTGTCATGACACCAGCCATG
GTGGAGCGCACCATGAAGGAGATCATCGAGCCGACGATCTCAGGCATGGCCAAGGATGGGAATCCTTTCTCCGGCGTATT
CTTCGCCGGCCTGATGATTACCGCCAAGGGTCCGGAACTGATCGAATACAATGTTCGCTTCGGCGATCCCGAATGCCAGG
TGCTGATGATGCGCCTCAAGAGTGACCTTTTGCCGATCCTCTACGCCACGGCCACCGGAACGCTGGACAAGGTGCAGGCG
GAATGGCGCGACGATGCGGCGCTGACCGTGGTTCTCGCTTCCAAGGGTTATCCCGGCGCTTATGACAAGAACACGCCGAT
TGCCCATATTCCCGAGGCCAGCGAAGAAGCCAAGGTGTTCCATGCCGGGACTGCGCTGAAGGATGGCAAACTCGTTGCGA
CCGGCGGCCGTGTGCTCAATGTCACCGCCTTTGGCAGAAACGTGACGGAAGCGCAGGCGCGCGCCTATGCGCTGGCCGAC
AAGGTTGAATGGGAAAACGGCTTCTGCCGCCGCGATATCGGCTGGCAGGCCATTGCCCGCGAAAAGGCCTGA

Upstream 100 bases:

>100_bases
GAAAGACAGGCATTTGCGGCGCTTTCTCCTTGACCTTTCGCCCCCCTGCCCTTAACGCCCGTGCCACAAGGTTACTGGCG
AATTTGCAGGGGTATTGACC

Downstream 100 bases:

>100_bases
TCCTTAAAGCAGATGGGCTCAGCAGCGACTATTTCGTTCAATTTTTACCAAGTTTGCTGACCCGATGGAAACGAAAGACC
GGTAATACTTGCCCCATTAT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MKVLLIGSGGREHALAWKIAQSPLIDALYAAPGNPGIADHATLVSLDVEDHAAVIAFAKEKAIDFVVIGPEAPLVAGLAD
DLRAAGIATFGPSKAAAQLEGSKGFTKDLCARYDIPTGAYERFKAAEPAKDYVRAQGAPIVIKADGLAAGKGVTVAMTEA
EALAAIDDCFDGAFGTAGTEVVVEAFLDGEEASFFCLSDGKTALALATAQDHKRVGDGDTGPNTGGMGAYSPAPVMTPAM
VERTMKEIIEPTISGMAKDGNPFSGVFFAGLMITAKGPELIEYNVRFGDPECQVLMMRLKSDLLPILYATATGTLDKVQA
EWRDDAALTVVLASKGYPGAYDKNTPIAHIPEASEEAKVFHAGTALKDGKLVATGGRVLNVTAFGRNVTEAQARAYALAD
KVEWENGFCRRDIGWQAIAREKA

Sequences:

>Translated_423_residues
MKVLLIGSGGREHALAWKIAQSPLIDALYAAPGNPGIADHATLVSLDVEDHAAVIAFAKEKAIDFVVIGPEAPLVAGLAD
DLRAAGIATFGPSKAAAQLEGSKGFTKDLCARYDIPTGAYERFKAAEPAKDYVRAQGAPIVIKADGLAAGKGVTVAMTEA
EALAAIDDCFDGAFGTAGTEVVVEAFLDGEEASFFCLSDGKTALALATAQDHKRVGDGDTGPNTGGMGAYSPAPVMTPAM
VERTMKEIIEPTISGMAKDGNPFSGVFFAGLMITAKGPELIEYNVRFGDPECQVLMMRLKSDLLPILYATATGTLDKVQA
EWRDDAALTVVLASKGYPGAYDKNTPIAHIPEASEEAKVFHAGTALKDGKLVATGGRVLNVTAFGRNVTEAQARAYALAD
KVEWENGFCRRDIGWQAIAREKA
>Mature_423_residues
MKVLLIGSGGREHALAWKIAQSPLIDALYAAPGNPGIADHATLVSLDVEDHAAVIAFAKEKAIDFVVIGPEAPLVAGLAD
DLRAAGIATFGPSKAAAQLEGSKGFTKDLCARYDIPTGAYERFKAAEPAKDYVRAQGAPIVIKADGLAAGKGVTVAMTEA
EALAAIDDCFDGAFGTAGTEVVVEAFLDGEEASFFCLSDGKTALALATAQDHKRVGDGDTGPNTGGMGAYSPAPVMTPAM
VERTMKEIIEPTISGMAKDGNPFSGVFFAGLMITAKGPELIEYNVRFGDPECQVLMMRLKSDLLPILYATATGTLDKVQA
EWRDDAALTVVLASKGYPGAYDKNTPIAHIPEASEEAKVFHAGTALKDGKLVATGGRVLNVTAFGRNVTEAQARAYALAD
KVEWENGFCRRDIGWQAIAREKA

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI4503915, Length=424, Percent_Identity=47.4056603773585, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI209869995, Length=424, Percent_Identity=47.4056603773585, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI209869993, Length=424, Percent_Identity=47.4056603773585, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI28416899, Length=424, Percent_Identity=47.4056603773585, Blast_Score=392, Evalue=1e-109,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=57.2429906542056, Blast_Score=476, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI17567511, Length=421, Percent_Identity=41.0926365795724, Blast_Score=308, Evalue=4e-84,
Organism=Saccharomyces cerevisiae, GI6321203, Length=444, Percent_Identity=40.5405405405405, Blast_Score=314, Evalue=2e-86,
Organism=Drosophila melanogaster, GI62471619, Length=413, Percent_Identity=45.0363196125908, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24582400, Length=413, Percent_Identity=45.0363196125908, Blast_Score=326, Evalue=2e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR2_AGRT5 (Q8UHN3)

Other databases:

- EMBL:   AE007869
- PIR:   AI2655
- PIR:   F97437
- RefSeq:   NP_353670.2
- ProteinModelPortal:   Q8UHN3
- SMR:   Q8UHN3
- STRING:   Q8UHN3
- GeneID:   1132685
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0647
- eggNOG:   COG0151
- HOGENOM:   HBG404060
- OMA:   GVPIVIK
- PhylomeDB:   Q8UHN3
- ProtClustDB:   PRK00885
- BioCyc:   ATUM176299-1:ATU0647-MONOMER
- HAMAP:   MF_00138
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.90.600.10
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR00877

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.13

Molecular weight: Translated: 44339; Mature: 44339

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS50975 ATP_GRASP; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLLIGSGGREHALAWKIAQSPLIDALYAAPGNPGIADHATLVSLDVEDHAAVIAFAKE
CEEEEEECCCCCCEEEEEECCCCHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEEEEECC
KAIDFVVIGPEAPLVAGLADDLRAAGIATFGPSKAAAQLEGSKGFTKDLCARYDIPTGAY
CCEEEEEECCCCCEECCCHHHHHHCCEEECCCCCHHHEECCCCCCHHHHHHHCCCCCCHH
ERFKAAEPAKDYVRAQGAPIVIKADGLAAGKGVTVAMTEAEALAAIDDCFDGAFGTAGTE
HHHCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEEEECHHHHHHHHHHHCCCCCCCCHH
VVVEAFLDGEEASFFCLSDGKTALALATAQDHKRVGDGDTGPNTGGMGAYSPAPVMTPAM
HHHHHHCCCCCCEEEEECCCCEEEEEEECHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHH
VERTMKEIIEPTISGMAKDGNPFSGVFFAGLMITAKGPELIEYNVRFGDPECQVLMMRLK
HHHHHHHHHHHHHHCCCCCCCCCCHHEEEEEEEEECCCCEEEEEEEECCCHHHHHHHHHH
SDLLPILYATATGTLDKVQAEWRDDAALTVVLASKGYPGAYDKNTPIAHIPEASEEAKVF
HCCCEEEEEECCCCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCEEECCCCCCCCEEE
HAGTALKDGKLVATGGRVLNVTAFGRNVTEAQARAYALADKVEWENGFCRRDIGWQAIAR
ECCCCCCCCEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHC
EKA
CCC
>Mature Secondary Structure
MKVLLIGSGGREHALAWKIAQSPLIDALYAAPGNPGIADHATLVSLDVEDHAAVIAFAKE
CEEEEEECCCCCCEEEEEECCCCHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEEEEECC
KAIDFVVIGPEAPLVAGLADDLRAAGIATFGPSKAAAQLEGSKGFTKDLCARYDIPTGAY
CCEEEEEECCCCCEECCCHHHHHHCCEEECCCCCHHHEECCCCCCHHHHHHHCCCCCCHH
ERFKAAEPAKDYVRAQGAPIVIKADGLAAGKGVTVAMTEAEALAAIDDCFDGAFGTAGTE
HHHCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEEEECHHHHHHHHHHHCCCCCCCCHH
VVVEAFLDGEEASFFCLSDGKTALALATAQDHKRVGDGDTGPNTGGMGAYSPAPVMTPAM
HHHHHHCCCCCCEEEEECCCCEEEEEEECHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHH
VERTMKEIIEPTISGMAKDGNPFSGVFFAGLMITAKGPELIEYNVRFGDPECQVLMMRLK
HHHHHHHHHHHHHHCCCCCCCCCCHHEEEEEEEEECCCCEEEEEEEECCCHHHHHHHHHH
SDLLPILYATATGTLDKVQAEWRDDAALTVVLASKGYPGAYDKNTPIAHIPEASEEAKVF
HCCCEEEEEECCCCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCEEECCCCCCCCEEE
HAGTALKDGKLVATGGRVLNVTAFGRNVTEAQARAYALADKVEWENGFCRRDIGWQAIAR
ECCCCCCCCEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHC
EKA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194