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The map label for this gene is rph

Identifier: 159184261

GI number: 159184261

Start: 321046

End: 321762

Strand: Reverse

Name: rph

Synonym: Atu0327

Alternate gene names: 159184261

Gene position: 321762-321046 (Counterclockwise)

Preceding gene: 15887681

Following gene: 159184260

Centisome position: 11.32

GC content: 60.11

Gene sequence:

>717_bases
ATGCGGCCTTCAGGCAGAAAAACCGACCAGATGCGCAAGGTTTCCTTCGAGCGCAATTTCTCGAAGCATGCCGAAGGCTC
CTGTCTCGTGAAATTCGGCGACACGCATGTGCTCGTCACCGCAAGCCTTGAAGAAAAGACGCCGCCGTGGCTGCGCAACA
GCGGCAAGGGCTGGGTCACCGCTGAATACGGCATGCTGCCACGCTCTACCAATGAACGCATGAAGCGTGAGGCTGCTTCC
GGCAAGCAGGGCGGCCGCACGCAGGAAATCCAGCGCCTCATCGGCCGCTCGCTGCGCGCCGTCGTTGACCTGCAGGCCCT
TGGCGAACGCCAGATCAGCATCGACTGCGATGTCATCCAGGCCGATGGCGGCACCCGCACCGCCTCCATTACCGGTGCAT
GGATTGCCCTGCATGACTGCCTGAAGTGGATGGAAACCCGTAACATGATCAAGGTCGAGAAGGTTCTGAAGGACCATATC
GCCGCGATCTCCTGCGGTATCTTCGCCAAGCAGCCGGTGATCGATCTCGATTATCTGGAAGACTCTTCCGCCGAGACCGA
CGCGAATTTCGTGATTACCGGTTCCGGCGGCATCGTCGAGGTTCAGGGAACGGCGGAAGGTGCGCCCTTCTCGGAGGAAG
AGTTCCTGACGCTGCTCGGTCTTGCCAAGGCCGGTTGCAGCGAACTGGTTGCCCTTCAGAAACAGGCAATCGCCTGA

Upstream 100 bases:

>100_bases
CAATATAATGCGTTGCAACGCCGGGGCAAAACGGAAATTGGCTTTGCAAAAGCCCGGAGCGCACCTTAGAAGGCAGGAAC
CAACATGGAGAGTGGATCAT

Downstream 100 bases:

>100_bases
GCCGACAGGAAAACGTCATGCGCAAGCTCGATACCAGAACGATCGTCGTCGCCAGCCACAACAAGGGCAAGATCGCCGAG
ATCGCCGATCTGATCGGGCC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRPSGRKTDQMRKVSFERNFSKHAEGSCLVKFGDTHVLVTASLEEKTPPWLRNSGKGWVTAEYGMLPRSTNERMKREAAS
GKQGGRTQEIQRLIGRSLRAVVDLQALGERQISIDCDVIQADGGTRTASITGAWIALHDCLKWMETRNMIKVEKVLKDHI
AAISCGIFAKQPVIDLDYLEDSSAETDANFVITGSGGIVEVQGTAEGAPFSEEEFLTLLGLAKAGCSELVALQKQAIA

Sequences:

>Translated_238_residues
MRPSGRKTDQMRKVSFERNFSKHAEGSCLVKFGDTHVLVTASLEEKTPPWLRNSGKGWVTAEYGMLPRSTNERMKREAAS
GKQGGRTQEIQRLIGRSLRAVVDLQALGERQISIDCDVIQADGGTRTASITGAWIALHDCLKWMETRNMIKVEKVLKDHI
AAISCGIFAKQPVIDLDYLEDSSAETDANFVITGSGGIVEVQGTAEGAPFSEEEFLTLLGLAKAGCSELVALQKQAIA
>Mature_238_residues
MRPSGRKTDQMRKVSFERNFSKHAEGSCLVKFGDTHVLVTASLEEKTPPWLRNSGKGWVTAEYGMLPRSTNERMKREAAS
GKQGGRTQEIQRLIGRSLRAVVDLQALGERQISIDCDVIQADGGTRTASITGAWIALHDCLKWMETRNMIKVEKVLKDHI
AAISCGIFAKQPVIDLDYLEDSSAETDANFVITGSGGIVEVQGTAEGAPFSEEEFLTLLGLAKAGCSELVALQKQAIA

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=61.6113744075829, Blast_Score=266, Evalue=8e-73,
Organism=Caenorhabditis elegans, GI71981632, Length=182, Percent_Identity=26.9230769230769, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_AGRT5 (Q8UIG8)

Other databases:

- EMBL:   AE007869
- PIR:   AG2616
- PIR:   G97398
- RefSeq:   NP_353359.2
- ProteinModelPortal:   Q8UIG8
- SMR:   Q8UIG8
- STRING:   Q8UIG8
- GeneID:   1132365
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0327
- eggNOG:   COG0689
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- PhylomeDB:   Q8UIG8
- ProtClustDB:   PRK00173
- BioCyc:   ATUM176299-1:ATU0327-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25968; Mature: 25968

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSGRKTDQMRKVSFERNFSKHAEGSCLVKFGDTHVLVTASLEEKTPPWLRNSGKGWVT
CCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCEEEEEEEECCCCCCCHHHCCCCCEEE
AEYGMLPRSTNERMKREAASGKQGGRTQEIQRLIGRSLRAVVDLQALGERQISIDCDVIQ
EECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEE
ADGGTRTASITGAWIALHDCLKWMETRNMIKVEKVLKDHIAAISCGIFAKQPVIDLDYLE
CCCCCEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCC
DSSAETDANFVITGSGGIVEVQGTAEGAPFSEEEFLTLLGLAKAGCSELVALQKQAIA
CCCCCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRPSGRKTDQMRKVSFERNFSKHAEGSCLVKFGDTHVLVTASLEEKTPPWLRNSGKGWVT
CCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCEEEEEEEECCCCCCCHHHCCCCCEEE
AEYGMLPRSTNERMKREAASGKQGGRTQEIQRLIGRSLRAVVDLQALGERQISIDCDVIQ
EECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEE
ADGGTRTASITGAWIALHDCLKWMETRNMIKVEKVLKDHIAAISCGIFAKQPVIDLDYLE
CCCCCEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCC
DSSAETDANFVITGSGGIVEVQGTAEGAPFSEEEFLTLLGLAKAGCSELVALQKQAIA
CCCCCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194