Click here to switch to the map view.

The map label for this gene is ptsN [H]

Identifier: 15887681

GI number: 15887681

Start: 323822

End: 324283

Strand: Reverse

Name: ptsN [H]

Synonym: Atu0330

Alternate gene names: 15887681

Gene position: 324283-323822 (Counterclockwise)

Preceding gene: 159184262

Following gene: 159184261

Centisome position: 11.41

GC content: 58.66

Gene sequence:

>462_bases
ATGGCGTTGGCAGATTTGCTGCAACAAGATGCGATTATTCCCGCCCTCAAGGTGAATTCCAAAAAGCAGTTGCTTCAGGA
GCTGGCCGCAAAGGCAGCCAGAATTACCGGGGTCTCGGAACGGGAAGTTTTCGACGTCATCCTCCAGCGTGAGAAACTCG
GCTCCACCGGTGTGGGTCATGGCATTGCCATTCCGCATGGCAAGCTCAACAGCATTCATCAGATTACCGGCGTCTTCGCA
CGTCTTGAAACCCCGGTCGATTTCGAAGCTTTGGACGACCAGCCGGTCGATCTGGTTTTCCTGCTGCTCGCGCCAGAAGG
CGCTGGCGCGGATCACCTGAAGGCGCTGTCGCGCATTGCGCGGGCGCTACGCGATCCTGAACTGGTGACCAAGCTGCGCG
CCACCGATTCCGACACGGCGATCTACGCCTTCCTCAATCAGGAACAGGCGACGGCGGCCTGA

Upstream 100 bases:

>100_bases
GAAACGATCAAGAGCTGAACAGTCTGACAAGACGTGGGAGGCAGAGCGCCAAGGCGCATCTGCCTCCTGCATCGTCTCGA
TGGCATGAAGGAAAAAGAGA

Downstream 100 bases:

>100_bases
TCGCGGGCCTATCCTGTCAGTTTAACGACAATAAAAAACGCGCCCTGAAGGCGCGTTTTTTATGACGGAAGGATAAATGC
CTCACGCGTCCTTTTCATTA

Product: nitrogen regulatory IIA protein

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Nitrogen-metabolic PTS system EIIA component [H]

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MALADLLQQDAIIPALKVNSKKQLLQELAAKAARITGVSEREVFDVILQREKLGSTGVGHGIAIPHGKLNSIHQITGVFA
RLETPVDFEALDDQPVDLVFLLLAPEGAGADHLKALSRIARALRDPELVTKLRATDSDTAIYAFLNQEQATAA

Sequences:

>Translated_153_residues
MALADLLQQDAIIPALKVNSKKQLLQELAAKAARITGVSEREVFDVILQREKLGSTGVGHGIAIPHGKLNSIHQITGVFA
RLETPVDFEALDDQPVDLVFLLLAPEGAGADHLKALSRIARALRDPELVTKLRATDSDTAIYAFLNQEQATAA
>Mature_152_residues
ALADLLQQDAIIPALKVNSKKQLLQELAAKAARITGVSEREVFDVILQREKLGSTGVGHGIAIPHGKLNSIHQITGVFAR
LETPVDFEALDDQPVDLVFLLLAPEGAGADHLKALSRIARALRDPELVTKLRATDSDTAIYAFLNQEQATAA

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=152, Percent_Identity=40.7894736842105, Blast_Score=118, Evalue=1e-28,
Organism=Escherichia coli, GI2367327, Length=130, Percent_Identity=31.5384615384615, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1786951, Length=154, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16470; Mature: 16339

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALADLLQQDAIIPALKVNSKKQLLQELAAKAARITGVSEREVFDVILQREKLGSTGVGH
CCHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC
GIAIPHGKLNSIHQITGVFARLETPVDFEALDDQPVDLVFLLLAPEGAGADHLKALSRIA
CEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHH
RALRDPELVTKLRATDSDTAIYAFLNQEQATAA
HHHCCHHHHHHHHCCCCCCEEEEEECCHHHCCC
>Mature Secondary Structure 
ALADLLQQDAIIPALKVNSKKQLLQELAAKAARITGVSEREVFDVILQREKLGSTGVGH
CHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC
GIAIPHGKLNSIHQITGVFARLETPVDFEALDDQPVDLVFLLLAPEGAGADHLKALSRIA
CEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHH
RALRDPELVTKLRATDSDTAIYAFLNQEQATAA
HHHCCHHHHHHHHCCCCCCEEEEEECCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275; 1991712 [H]