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The map label for this gene is dnaA [H]

Identifier: 159184258

GI number: 159184258

Start: 317547

End: 319109

Strand: Direct

Name: dnaA [H]

Synonym: Atu0324

Alternate gene names: 159184258

Gene position: 317547-319109 (Clockwise)

Preceding gene: 15887673

Following gene: 15887679

Centisome position: 11.18

GC content: 59.18

Gene sequence:

>1563_bases
ATGCAATTGAATTTGGCGATGGGTGCGGTGGCTGATGGGGACCGCGCACCAAAGGCATGTGATGCAGCTTGCTCAGAAGC
GGCAGGGGATAAATCGGCAATGATGCATGATGCGCTGTTTGAACGTTTTAGCGCGCGGCTCAAGGCACAGGTTGGTCCCG
AGGTCTATGCAAGCTGGTTTGCACGGCTCAAACTCCATACCGTTTCGAAAAGCGTCGTCCGCTTTACCGTTCCGACGACC
TTTCTGAAGTCCTGGATCAACAACCGTTACATGGATCTGATCACCAGCCTCGTTCAGAGCGAGGATCCGGATGTGCTGAA
GGTGGAGATTCTTGTCCGCTCGGCCAGCCGTCCGGTTCGCCCGGCGCAGACCGAAGAACGGGCGCAGCCCGTTCAGGAGG
TTGGTGCGGCTCCGCGTAACAAGTCCTTCATTCCGTCGCAGTCCGCGACAGCGCCTGCGGCTCAGCCTATGGCGGCGCAG
GCGACCCTGCGTCAGGGTGGTTCCGGGCCGCTGTTCGGCTCGCCGCTCGATACACGCTTTACCTTTGATACCTTTGTTGA
AGGGTCGTCCAACCGTGTCGCCCTTGCGGCGGCAAAAACGATCGCCGAAGCTGGCGCTGGCGCCGTGCGCTTCAATCCGC
TGTTCATTCATGCGGGGGTCGGCCTCGGTAAGACGCATCTTCTGCAGGCCATTGCCAATGCAGCCATCGACAGCCCGCGC
AATCCGCGCGTGGTCTATCTGACGGCCGAATATTTCATGTGGCGTTTTGCGACTGCGATCCGCGACAATGACGCGCTGAC
GCTGAAGGACACGCTGCGCAATATCGACCTTCTCGTCATCGACGACATGCAGTTCCTGCAGGGCAAGATGATCCAGCACG
AATTCTGCCACCTGCTGAACATGCTGCTCGACAGCGCCAAGCAGGTCGTGGTTGCGGCTGACCGTGCGCCCTGGGAGCTG
GAGTCGCTCGATCCGCGTGTCCGTTCCCGTCTTCAGGGCGGTATGGCGATCGAGATCGAAGGCCCTGATTACGACATGCG
CTACGAAATGCTCAACCGTCGCATGGGCTCTGCGCGTCAGGATGATCCGTCTTTCGAAATTTCGGACGAAATTCTGACCC
ATGTGGCCAAAAGCGTGACGGCGAGCGGTCGCGAGCTTGAAGGCGCCTTCAACCAGCTGATGTTCCGACGCTCTTTCGAG
CCGAACCTCTCGGTTGACCGTGTCGATGAACTGCTGTCGCACCTCGTTGGCAGCGGTGAGGCCAAGCGTGTCCGTATCGA
GGATATTCAGCGCATCGTCGCCAGGCACTATAATGTGTCGCGGCAGGAACTGGTGTCCAACCGTCGCACCCGCGTCATCG
TCAAACCGCGCCAGATCGCCATGTATCTGGCCAAGATGTTGACGCCGCGCTCATTTCCCGAGATCGGTCGTCGTTTCGGC
GGTCGCGACCACACCACGGTTCTTCACGCGGTGCGCAAGATCGAGGATCTGATTTCCGGCGATACAAAGCTCGGCCACGA
GGTTGAGCTACTGAAACGCCTGATCAACGAAAACAACGCATGA

Upstream 100 bases:

>100_bases
TTCTTTCGAGGGGAACGCTGGGGGCAAAATAAACGAGGGGGATCCCTGGCGGTCGATATTTGATCGTCAGGCTATGATTG
GCAATTTGAAAGGCGGCAAT

Downstream 100 bases:

>100_bases
AAAAAAGCGGCCTTCGGGCCGCTTTTTTATTGAATTCTCGTTATCGGGTTCAGCCGGGTGGCGCTGGCCTCAGCAGGCGA
GATCGGCCACCACGGCATCC

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MQLNLAMGAVADGDRAPKACDAACSEAAGDKSAMMHDALFERFSARLKAQVGPEVYASWFARLKLHTVSKSVVRFTVPTT
FLKSWINNRYMDLITSLVQSEDPDVLKVEILVRSASRPVRPAQTEERAQPVQEVGAAPRNKSFIPSQSATAPAAQPMAAQ
ATLRQGGSGPLFGSPLDTRFTFDTFVEGSSNRVALAAAKTIAEAGAGAVRFNPLFIHAGVGLGKTHLLQAIANAAIDSPR
NPRVVYLTAEYFMWRFATAIRDNDALTLKDTLRNIDLLVIDDMQFLQGKMIQHEFCHLLNMLLDSAKQVVVAADRAPWEL
ESLDPRVRSRLQGGMAIEIEGPDYDMRYEMLNRRMGSARQDDPSFEISDEILTHVAKSVTASGRELEGAFNQLMFRRSFE
PNLSVDRVDELLSHLVGSGEAKRVRIEDIQRIVARHYNVSRQELVSNRRTRVIVKPRQIAMYLAKMLTPRSFPEIGRRFG
GRDHTTVLHAVRKIEDLISGDTKLGHEVELLKRLINENNA

Sequences:

>Translated_520_residues
MQLNLAMGAVADGDRAPKACDAACSEAAGDKSAMMHDALFERFSARLKAQVGPEVYASWFARLKLHTVSKSVVRFTVPTT
FLKSWINNRYMDLITSLVQSEDPDVLKVEILVRSASRPVRPAQTEERAQPVQEVGAAPRNKSFIPSQSATAPAAQPMAAQ
ATLRQGGSGPLFGSPLDTRFTFDTFVEGSSNRVALAAAKTIAEAGAGAVRFNPLFIHAGVGLGKTHLLQAIANAAIDSPR
NPRVVYLTAEYFMWRFATAIRDNDALTLKDTLRNIDLLVIDDMQFLQGKMIQHEFCHLLNMLLDSAKQVVVAADRAPWEL
ESLDPRVRSRLQGGMAIEIEGPDYDMRYEMLNRRMGSARQDDPSFEISDEILTHVAKSVTASGRELEGAFNQLMFRRSFE
PNLSVDRVDELLSHLVGSGEAKRVRIEDIQRIVARHYNVSRQELVSNRRTRVIVKPRQIAMYLAKMLTPRSFPEIGRRFG
GRDHTTVLHAVRKIEDLISGDTKLGHEVELLKRLINENNA
>Mature_520_residues
MQLNLAMGAVADGDRAPKACDAACSEAAGDKSAMMHDALFERFSARLKAQVGPEVYASWFARLKLHTVSKSVVRFTVPTT
FLKSWINNRYMDLITSLVQSEDPDVLKVEILVRSASRPVRPAQTEERAQPVQEVGAAPRNKSFIPSQSATAPAAQPMAAQ
ATLRQGGSGPLFGSPLDTRFTFDTFVEGSSNRVALAAAKTIAEAGAGAVRFNPLFIHAGVGLGKTHLLQAIANAAIDSPR
NPRVVYLTAEYFMWRFATAIRDNDALTLKDTLRNIDLLVIDDMQFLQGKMIQHEFCHLLNMLLDSAKQVVVAADRAPWEL
ESLDPRVRSRLQGGMAIEIEGPDYDMRYEMLNRRMGSARQDDPSFEISDEILTHVAKSVTASGRELEGAFNQLMFRRSFE
PNLSVDRVDELLSHLVGSGEAKRVRIEDIQRIVARHYNVSRQELVSNRRTRVIVKPRQIAMYLAKMLTPRSFPEIGRRFG
GRDHTTVLHAVRKIEDLISGDTKLGHEVELLKRLINENNA

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=474, Percent_Identity=34.3881856540084, Blast_Score=270, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 57832; Mature: 57832

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLNLAMGAVADGDRAPKACDAACSEAAGDKSAMMHDALFERFSARLKAQVGPEVYASWF
CEEEEEECCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ARLKLHTVSKSVVRFTVPTTFLKSWINNRYMDLITSLVQSEDPDVLKVEILVRSASRPVR
HHHHHHHHHHHHHEEECCHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCC
PAQTEERAQPVQEVGAAPRNKSFIPSQSATAPAAQPMAAQATLRQGGSGPLFGSPLDTRF
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCE
TFDTFVEGSSNRVALAAAKTIAEAGAGAVRFNPLFIHAGVGLGKTHLLQAIANAAIDSPR
EHHHHHCCCCCEEEHHHHHHHHHCCCCEEEECEEEEECCCCCCHHHHHHHHHHHHCCCCC
NPRVVYLTAEYFMWRFATAIRDNDALTLKDTLRNIDLLVIDDMQFLQGKMIQHEFCHLLN
CCEEEEEEHHHHHHHHHHHHCCCCCEEHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHH
MLLDSAKQVVVAADRAPWELESLDPRVRSRLQGGMAIEIEGPDYDMRYEMLNRRMGSARQ
HHHHCCHHEEEEECCCCCCHHCCCHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCC
DDPSFEISDEILTHVAKSVTASGRELEGAFNQLMFRRSFEPNLSVDRVDELLSHLVGSGE
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
AKRVRIEDIQRIVARHYNVSRQELVSNRRTRVIVKPRQIAMYLAKMLTPRSFPEIGRRFG
CCEEEHHHHHHHHHHHCCCCHHHHHCCCCCEEEECHHHHHHHHHHHHCCCCCHHHHHHCC
GRDHTTVLHAVRKIEDLISGDTKLGHEVELLKRLINENNA
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQLNLAMGAVADGDRAPKACDAACSEAAGDKSAMMHDALFERFSARLKAQVGPEVYASWF
CEEEEEECCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ARLKLHTVSKSVVRFTVPTTFLKSWINNRYMDLITSLVQSEDPDVLKVEILVRSASRPVR
HHHHHHHHHHHHHEEECCHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCC
PAQTEERAQPVQEVGAAPRNKSFIPSQSATAPAAQPMAAQATLRQGGSGPLFGSPLDTRF
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCE
TFDTFVEGSSNRVALAAAKTIAEAGAGAVRFNPLFIHAGVGLGKTHLLQAIANAAIDSPR
EHHHHHCCCCCEEEHHHHHHHHHCCCCEEEECEEEEECCCCCCHHHHHHHHHHHHCCCCC
NPRVVYLTAEYFMWRFATAIRDNDALTLKDTLRNIDLLVIDDMQFLQGKMIQHEFCHLLN
CCEEEEEEHHHHHHHHHHHHCCCCCEEHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHH
MLLDSAKQVVVAADRAPWELESLDPRVRSRLQGGMAIEIEGPDYDMRYEMLNRRMGSARQ
HHHHCCHHEEEEECCCCCCHHCCCHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCC
DDPSFEISDEILTHVAKSVTASGRELEGAFNQLMFRRSFEPNLSVDRVDELLSHLVGSGE
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
AKRVRIEDIQRIVARHYNVSRQELVSNRRTRVIVKPRQIAMYLAKMLTPRSFPEIGRRFG
CCEEEHHHHHHHHHHHCCCCHHHHHCCCCCEEEECHHHHHHHHHHHHCCCCCHHHHHHCC
GRDHTTVLHAVRKIEDLISGDTKLGHEVELLKRLINENNA
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]