| Definition | Agrobacterium tumefaciens str. C58 chromosome circular, complete sequence. |
|---|---|
| Accession | NC_003062 |
| Length | 2,841,580 |
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The map label for this gene is nrdE [H]
Identifier: 159184147
GI number: 159184147
Start: 71679
End: 73760
Strand: Direct
Name: nrdE [H]
Synonym: Atu0070
Alternate gene names: 159184147
Gene position: 71679-73760 (Clockwise)
Preceding gene: 15887422
Following gene: 159184148
Centisome position: 2.52
GC content: 55.0
Gene sequence:
>2082_bases ATGCTGAACCTTTATGACGAGAACGGAAAGATCCAGCTCGACAAGGACCGTCTGGCTGCCCGGCAATATTTCCTCCAGCA CGTCAACCAGAACACCGTCTTCTTCCATAATCTGCGGGAGAAGCTCGATTATCTGGTAACGGAGGGATATTACGAGCAGG AAGTGCTGGACCAGTACTCCTTCAATTTCGTCCGTGACCTCTATGACGAGGCCTATTCCCGGAAGTTTCGTTTTCCGACC TTCCTCGGCGCGTTCAAATATTACACGTCTTATACGCTGAAGACCTTCGACGGAAAACGTTATCTCGAGCGTTACGAGGA CCGGGTCTGCATGGTGGCGCTGGCTCTTGCGCGCGGCGACGAGCAGCTGGCCCGTGATCTCATGGACGAGATCATTTCCG GCCGTTTTCAGCCGGCAACCCCGACATTCCTCAACGCCGGCAAGAAAAGCCGCGGCGAGCTGGTCTCGTGTTTTCTGCTG CGCGTGGAAGACAATATGGAAAGCATTGGCCGCTCGATCAATTCGGCTCTCCAGCTTTCCAAGCGTGGCGGCGGTGTGGC GCTGTCCCTGACCAATATTCGCGAATTCGGGGCACCGATCAAGCAGATCGAAAACCAGTCTTCCGGCGTCATTCCTGTCA TGAAGCTTCTGGAAGACAGCTTTTCCTACGCCAACCAGTTGGGTGCACGCCAGGGCGCGGGCGCGGTCTATCTGCATGCC CACCACCCTGATATCATGCGTTTCCTCGATACCAAACGCGAAAATGCTGACGAAAAAATCCGTATCAAGACCCTGTCGCT CGGCGTCGTCATCCCCGACATTACCTTCGAGCTCGCCCGCAACAATGAGGATATGTACCTGTTCTCGCCCTATGATGTGG AGCGGGTCTATGGCGTGCCGTTGACGGAGATCTCGGTCACCGAAAAATATCACGAGATGGTTGCCGACAGCCGCATCCGC AAGAAGAAGATCAAGGCACGTGAATTCTTCCAGGTGATCGCCGAAATCCAGTTCGAAAGCGGTTACCCCTACATCATGTT CGAGGATACGGTGAACCGCGCCAATCCGGTCGCCGGCCGCATCAGCATGAGCAATCTCTGCTCGGAAATTTTGCAGGTCA GCGAAGCGAGCGAGTTCAACGCCGATCTCTCCTATAGCCATATGGGCAAGGATATCTCGTGCAATCTCGGCTCGCTCAAC ATTGCCTCTGCCATGGACTCCGCTGATTTCGGCAAGACGATCGAGACCTCCATCCGGGCACTGACCGCCGTATCGGACAT GAGTCATATTTCCTCCGTTCCTTCGGTGGAAAAGGGCAATGATGCCAGCCACGCCATCGGCCTTGGCCAGATGAACCTGC ACGGTTATCTGGCGCGTGAGCGCATCTTCTACGGTTCGGAGGAAGGTATCGACTTCACCAATATCTATTTCTACACCGTG ACCTATCACGCCATCCGTGCCAGCAACCGGCTCGCCGTGGAACGGGGCCAGAGTTTCAAGGGCTTTGAGAACTCCAAATA TGCCTCGGGCGACTATTTCGACAAATATACCGACCGGGAATGGTTGCCCGCGACGGAAAAGGTCCGCGACCTGTTCGAAA AGGCCGGTATTGCCATTCCGACGCAGGAAGACTGGACGGCGTTGAAACAGGACGTCATGGCATCAGGCCTCTATAACCAG AACCTTCAGGCCGTGCCGCCGACGGGCTCGATCTCCTATATCAACCACTCCACTTCTTCCATTCATCCGATCGTTTCGAA AATCGAAATTCGTAAAGAGGGTAAGATCGGTCGCGTTTATTACCCGGCTTCCTATCTCTCGAACGATAATCTCGAATATT ATCAGGATGCCTATGAGATCGGGCCGGAAAAGATCATCGACACCTATGCGGCGGCCACCCAGCATGTCGATCAGGGCCTG TCGCTGACGTTATTCTTCCGCGATACCGCCACGACGCGCGATATCAACCGGGCGCAGATTTACGCCTGGAGACGAGGCAT CAAGACCATCTATTACATTCGGTTGCGCCAGATGGCGCTGGAAGGCACGCAGGTTCAGGGCTGCGTTTCCTGCACGCTCT GA
Upstream 100 bases:
>100_bases CACGCTGACCTCAAGCGCGCTGCGCAAGGATGCCAAACAGCCCGTAGCCGCAAGCCAGAAACCGGTTGATACGGGACTGG ATTATCACGCGCTGAACGCG
Downstream 100 bases:
>100_bases TTTGAGGGACAAGAGAATGAACATCGCCGTAAAACCAGCGTCCCGCATCCGCGCGGTCAACTGGAACCGCATTGAGGATG ACAAGGACCTCGAAGTCTGG
Product: ribonucleotide-diphosphate reductase subunit alpha
Products: NA
Alternate protein names: Ribonucleotide reductase R1 subunit [H]
Number of amino acids: Translated: 693; Mature: 693
Protein sequence:
>693_residues MLNLYDENGKIQLDKDRLAARQYFLQHVNQNTVFFHNLREKLDYLVTEGYYEQEVLDQYSFNFVRDLYDEAYSRKFRFPT FLGAFKYYTSYTLKTFDGKRYLERYEDRVCMVALALARGDEQLARDLMDEIISGRFQPATPTFLNAGKKSRGELVSCFLL RVEDNMESIGRSINSALQLSKRGGGVALSLTNIREFGAPIKQIENQSSGVIPVMKLLEDSFSYANQLGARQGAGAVYLHA HHPDIMRFLDTKRENADEKIRIKTLSLGVVIPDITFELARNNEDMYLFSPYDVERVYGVPLTEISVTEKYHEMVADSRIR KKKIKAREFFQVIAEIQFESGYPYIMFEDTVNRANPVAGRISMSNLCSEILQVSEASEFNADLSYSHMGKDISCNLGSLN IASAMDSADFGKTIETSIRALTAVSDMSHISSVPSVEKGNDASHAIGLGQMNLHGYLARERIFYGSEEGIDFTNIYFYTV TYHAIRASNRLAVERGQSFKGFENSKYASGDYFDKYTDREWLPATEKVRDLFEKAGIAIPTQEDWTALKQDVMASGLYNQ NLQAVPPTGSISYINHSTSSIHPIVSKIEIRKEGKIGRVYYPASYLSNDNLEYYQDAYEIGPEKIIDTYAAATQHVDQGL SLTLFFRDTATTRDINRAQIYAWRRGIKTIYYIRLRQMALEGTQVQGCVSCTL
Sequences:
>Translated_693_residues MLNLYDENGKIQLDKDRLAARQYFLQHVNQNTVFFHNLREKLDYLVTEGYYEQEVLDQYSFNFVRDLYDEAYSRKFRFPT FLGAFKYYTSYTLKTFDGKRYLERYEDRVCMVALALARGDEQLARDLMDEIISGRFQPATPTFLNAGKKSRGELVSCFLL RVEDNMESIGRSINSALQLSKRGGGVALSLTNIREFGAPIKQIENQSSGVIPVMKLLEDSFSYANQLGARQGAGAVYLHA HHPDIMRFLDTKRENADEKIRIKTLSLGVVIPDITFELARNNEDMYLFSPYDVERVYGVPLTEISVTEKYHEMVADSRIR KKKIKAREFFQVIAEIQFESGYPYIMFEDTVNRANPVAGRISMSNLCSEILQVSEASEFNADLSYSHMGKDISCNLGSLN IASAMDSADFGKTIETSIRALTAVSDMSHISSVPSVEKGNDASHAIGLGQMNLHGYLARERIFYGSEEGIDFTNIYFYTV TYHAIRASNRLAVERGQSFKGFENSKYASGDYFDKYTDREWLPATEKVRDLFEKAGIAIPTQEDWTALKQDVMASGLYNQ NLQAVPPTGSISYINHSTSSIHPIVSKIEIRKEGKIGRVYYPASYLSNDNLEYYQDAYEIGPEKIIDTYAAATQHVDQGL SLTLFFRDTATTRDINRAQIYAWRRGIKTIYYIRLRQMALEGTQVQGCVSCTL >Mature_693_residues MLNLYDENGKIQLDKDRLAARQYFLQHVNQNTVFFHNLREKLDYLVTEGYYEQEVLDQYSFNFVRDLYDEAYSRKFRFPT FLGAFKYYTSYTLKTFDGKRYLERYEDRVCMVALALARGDEQLARDLMDEIISGRFQPATPTFLNAGKKSRGELVSCFLL RVEDNMESIGRSINSALQLSKRGGGVALSLTNIREFGAPIKQIENQSSGVIPVMKLLEDSFSYANQLGARQGAGAVYLHA HHPDIMRFLDTKRENADEKIRIKTLSLGVVIPDITFELARNNEDMYLFSPYDVERVYGVPLTEISVTEKYHEMVADSRIR KKKIKAREFFQVIAEIQFESGYPYIMFEDTVNRANPVAGRISMSNLCSEILQVSEASEFNADLSYSHMGKDISCNLGSLN IASAMDSADFGKTIETSIRALTAVSDMSHISSVPSVEKGNDASHAIGLGQMNLHGYLARERIFYGSEEGIDFTNIYFYTV TYHAIRASNRLAVERGQSFKGFENSKYASGDYFDKYTDREWLPATEKVRDLFEKAGIAIPTQEDWTALKQDVMASGLYNQ NLQAVPPTGSISYINHSTSSIHPIVSKIEIRKEGKIGRVYYPASYLSNDNLEYYQDAYEIGPEKIIDTYAAATQHVDQGL SLTLFFRDTATTRDINRAQIYAWRRGIKTIYYIRLRQMALEGTQVQGCVSCTL
Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]
COG id: COG0209
COG function: function code F; Ribonucleotide reductase, alpha subunit
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]
Homologues:
Organism=Homo sapiens, GI4506749, Length=656, Percent_Identity=26.5243902439024, Blast_Score=191, Evalue=3e-48, Organism=Escherichia coli, GI1789030, Length=693, Percent_Identity=72.4386724386724, Blast_Score=1080, Evalue=0.0, Organism=Escherichia coli, GI2367133, Length=581, Percent_Identity=25.1290877796902, Blast_Score=169, Evalue=4e-43, Organism=Caenorhabditis elegans, GI17555242, Length=654, Percent_Identity=26.1467889908257, Blast_Score=185, Evalue=6e-47, Organism=Saccharomyces cerevisiae, GI6320914, Length=655, Percent_Identity=27.175572519084, Blast_Score=189, Evalue=1e-48, Organism=Saccharomyces cerevisiae, GI6322123, Length=720, Percent_Identity=25.9722222222222, Blast_Score=185, Evalue=2e-47, Organism=Drosophila melanogaster, GI17352481, Length=653, Percent_Identity=26.3399693721286, Blast_Score=180, Evalue=3e-45,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013346 - InterPro: IPR013509 - InterPro: IPR000788 - InterPro: IPR008926 - InterPro: IPR013554 [H]
Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]
EC number: =1.17.4.1 [H]
Molecular weight: Translated: 78850; Mature: 78850
Theoretical pI: Translated: 6.19; Mature: 6.19
Prosite motif: PS00089 RIBORED_LARGE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.9 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 3.2 %Cys+Met (Translated Protein) 0.9 %Cys (Mature Protein) 2.3 %Met (Mature Protein) 3.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MLNLYDENGKIQLDKDRLAARQYFLQHVNQNTVFFHNLREKLDYLVTEGYYEQEVLDQYS CCCEEECCCCEEECHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHH FNFVRDLYDEAYSRKFRFPTFLGAFKYYTSYTLKTFDGKRYLERYEDRVCMVALALARGD HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHCCH EQLARDLMDEIISGRFQPATPTFLNAGKKSRGELVSCFLLRVEDNMESIGRSINSALQLS HHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCHHHEEHHEEHHHHHHHHHHHHHHHHHHHH KRGGGVALSLTNIREFGAPIKQIENQSSGVIPVMKLLEDSFSYANQLGARQGAGAVYLHA CCCCCEEEEECCHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE HHPDIMRFLDTKRENADEKIRIKTLSLGVVIPDITFELARNNEDMYLFSPYDVERVYGVP CCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCEEEEECCCCCEEEECCCCCHHCCCCC LTEISVTEKYHEMVADSRIRKKKIKAREFFQVIAEIQFESGYPYIMFEDTVNRANPVAGR CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCC ISMSNLCSEILQVSEASEFNADLSYSHMGKDISCNLGSLNIASAMDSADFGKTIETSIRA CCHHHHHHHHHHHHCCHHCCCCCCHHHCCCCCCCCCCCCCHHHHHCCHHCCCHHHHHHHH LTAVSDMSHISSVPSVEKGNDASHAIGLGQMNLHGYLARERIFYGSEEGIDFTNIYFYTV HHHHHHHHHHHCCCCCCCCCCCHHEEECCCCCHHHHHHHHHEEECCCCCCCEEEEEEEEE TYHAIRASNRLAVERGQSFKGFENSKYASGDYFDKYTDREWLPATEKVRDLFEKAGIAIP EEEEHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCC TQEDWTALKQDVMASGLYNQNLQAVPPTGSISYINHSTSSIHPIVSKIEIRKEGKIGRVY CCHHHHHHHHHHHHHCCCCCCCEECCCCCCEEEEECCCCHHHHHHHHHHEECCCCEEEEE YPASYLSNDNLEYYQDAYEIGPEKIIDTYAAATQHVDQGLSLTLFFRDTATTRDINRAQI ECHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHH YAWRRGIKTIYYIRLRQMALEGTQVQGCVSCTL HHHHHCCHHHHHHHHHHHHHCCCCCCCEEEECC >Mature Secondary Structure MLNLYDENGKIQLDKDRLAARQYFLQHVNQNTVFFHNLREKLDYLVTEGYYEQEVLDQYS CCCEEECCCCEEECHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHH FNFVRDLYDEAYSRKFRFPTFLGAFKYYTSYTLKTFDGKRYLERYEDRVCMVALALARGD HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHCCH EQLARDLMDEIISGRFQPATPTFLNAGKKSRGELVSCFLLRVEDNMESIGRSINSALQLS HHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCHHHEEHHEEHHHHHHHHHHHHHHHHHHHH KRGGGVALSLTNIREFGAPIKQIENQSSGVIPVMKLLEDSFSYANQLGARQGAGAVYLHA CCCCCEEEEECCHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE HHPDIMRFLDTKRENADEKIRIKTLSLGVVIPDITFELARNNEDMYLFSPYDVERVYGVP CCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCEEEEECCCCCEEEECCCCCHHCCCCC LTEISVTEKYHEMVADSRIRKKKIKAREFFQVIAEIQFESGYPYIMFEDTVNRANPVAGR CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCC ISMSNLCSEILQVSEASEFNADLSYSHMGKDISCNLGSLNIASAMDSADFGKTIETSIRA CCHHHHHHHHHHHHCCHHCCCCCCHHHCCCCCCCCCCCCCHHHHHCCHHCCCHHHHHHHH LTAVSDMSHISSVPSVEKGNDASHAIGLGQMNLHGYLARERIFYGSEEGIDFTNIYFYTV HHHHHHHHHHHCCCCCCCCCCCHHEEECCCCCHHHHHHHHHEEECCCCCCCEEEEEEEEE TYHAIRASNRLAVERGQSFKGFENSKYASGDYFDKYTDREWLPATEKVRDLFEKAGIAIP EEEEHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCC TQEDWTALKQDVMASGLYNQNLQAVPPTGSISYINHSTSSIHPIVSKIEIRKEGKIGRVY CCHHHHHHHHHHHHHCCCCCCCEECCCCCCEEEEECCCCHHHHHHHHHHEECCCCEEEEE YPASYLSNDNLEYYQDAYEIGPEKIIDTYAAATQHVDQGLSLTLFFRDTATTRDINRAQI ECHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHH YAWRRGIKTIYYIRLRQMALEGTQVQGCVSCTL HHHHHCCHHHHHHHHHHHHHCCCCCCCEEEECC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 12788972 [H]