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The map label for this gene is trpA

Identifier: 159184126

GI number: 159184126

Start: 16364

End: 17203

Strand: Direct

Name: trpA

Synonym: Atu0019

Alternate gene names: 159184126

Gene position: 16364-17203 (Clockwise)

Preceding gene: 159184125

Following gene: 15887379

Centisome position: 0.58

GC content: 59.17

Gene sequence:

>840_bases
ATGACTGTACGTATGGACAAGCGCTTTGCCGATCTGCGGGCTGAAAATCGCCCCGCCCTGATCACCTATTTCATGGGCGG
CGACCCGGATTTCCAGACCTCGCTTGGCATCATGAAGGCCCTGCCGGAAGCAGGCGCCGACGTTATCGAACTCGGCATGC
CGTTTTCCGATCCCATGGCGGATGGCCCGGCCATTCAGCTGGCCGGCCAGCGCGCGCTGAAGGGTGGACAGACACTGAAG
ACCACGCTCGATCTCGCCCGCGAGTTCCGCAAGCAGGACAATGCAACGCCGATCGTGATGATGGGCTATTATAATCCGAT
CTATATCTATGGCGTTGAAAAGTTTCTGGACGACGCAATTGCCAGTGGCATTGATGGCCTTATCGTGGTCGACCTGCCGC
CGGAAATGGACGATGAACTCTGCATTCCGGCGCTTGCGCGCGGCATCAATTTCATCCGTCTGGCGACGCCGACCACGGAT
GGCAAGCGCCTGCCGGCCGTCCTTAAAAATACCTCAGGTTTCGTGTATTACGTCTCGATGAACGGTATCACCGGTTCAGC
ACTTCCCGATCCCTCGTTGATCTCGGGCGCTGTCGGTCGTATCAAGGCGCATACGGAACTGCCGGTTTGTGTCGGTTTTG
GCGTCAAGACGGCCGATCATGCGAAGGCGATCGGTGCCGTAGCGGATGGTGTGGTGGTGGGTTCAGCCATCGTCAACCAG
ATTGCGGGCAGCCTGACCAAGGACGGGCAGGCGACCGCCGACACTGTGCCGGCAGTTACGACGCTGGTAAAAGGACTTTC
AACGGGCGTGCGTGCATCGCGCCTCGCCGCTGCGGAATAA

Upstream 100 bases:

>100_bases
AAAATGGGCAAGGATGAAATCATCCTGATGAACCTCTCCGGCCGTGGCGACAAAGATGTCCACACCGTCAGCAAGTTCCT
TGGAATGGATGTATAAGATC

Downstream 100 bases:

>100_bases
GCGCGCGGCCCAGTCAGGAGTAGACAGTTGAACTGGATCACCAATTACGTTCGGCCGCGGATCAATTCCATGCTTGGCCG
CCGCCCGGAGGTTCCGGAGA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MTVRMDKRFADLRAENRPALITYFMGGDPDFQTSLGIMKALPEAGADVIELGMPFSDPMADGPAIQLAGQRALKGGQTLK
TTLDLAREFRKQDNATPIVMMGYYNPIYIYGVEKFLDDAIASGIDGLIVVDLPPEMDDELCIPALARGINFIRLATPTTD
GKRLPAVLKNTSGFVYYVSMNGITGSALPDPSLISGAVGRIKAHTELPVCVGFGVKTADHAKAIGAVADGVVVGSAIVNQ
IAGSLTKDGQATADTVPAVTTLVKGLSTGVRASRLAAAE

Sequences:

>Translated_279_residues
MTVRMDKRFADLRAENRPALITYFMGGDPDFQTSLGIMKALPEAGADVIELGMPFSDPMADGPAIQLAGQRALKGGQTLK
TTLDLAREFRKQDNATPIVMMGYYNPIYIYGVEKFLDDAIASGIDGLIVVDLPPEMDDELCIPALARGINFIRLATPTTD
GKRLPAVLKNTSGFVYYVSMNGITGSALPDPSLISGAVGRIKAHTELPVCVGFGVKTADHAKAIGAVADGVVVGSAIVNQ
IAGSLTKDGQATADTVPAVTTLVKGLSTGVRASRLAAAE
>Mature_278_residues
TVRMDKRFADLRAENRPALITYFMGGDPDFQTSLGIMKALPEAGADVIELGMPFSDPMADGPAIQLAGQRALKGGQTLKT
TLDLAREFRKQDNATPIVMMGYYNPIYIYGVEKFLDDAIASGIDGLIVVDLPPEMDDELCIPALARGINFIRLATPTTDG
KRLPAVLKNTSGFVYYVSMNGITGSALPDPSLISGAVGRIKAHTELPVCVGFGVKTADHAKAIGAVADGVVVGSAIVNQI
AGSLTKDGQATADTVPAVTTLVKGLSTGVRASRLAAAE

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=274, Percent_Identity=30.2919708029197, Blast_Score=121, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6321412, Length=239, Percent_Identity=42.2594142259414, Blast_Score=196, Evalue=3e-51,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_AGRT5 (Q8UJA9)

Other databases:

- EMBL:   AE007869
- PIR:   C97361
- RefSeq:   NP_353059.2
- ProteinModelPortal:   Q8UJA9
- SMR:   Q8UJA9
- STRING:   Q8UJA9
- GeneID:   1132057
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0019
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- PhylomeDB:   Q8UJA9
- ProtClustDB:   PRK13111
- BioCyc:   ATUM176299-1:ATU0019-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 29269; Mature: 29138

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 50-50 ACT_SITE 61-61

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRMDKRFADLRAENRPALITYFMGGDPDFQTSLGIMKALPEAGADVIELGMPFSDPMA
CCEECCHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
DGPAIQLAGQRALKGGQTLKTTLDLAREFRKQDNATPIVMMGYYNPIYIYGVEKFLDDAI
CCCEEEECCHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEHHHHHHHHH
ASGIDGLIVVDLPPEMDDELCIPALARGINFIRLATPTTDGKRLPAVLKNTSGFVYYVSM
HCCCCCEEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCHHHHCCCCCEEEEEEE
NGITGSALPDPSLISGAVGRIKAHTELPVCVGFGVKTADHAKAIGAVADGVVVGSAIVNQ
CCCCCCCCCCCHHHHHHHHHEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
IAGSLTKDGQATADTVPAVTTLVKGLSTGVRASRLAAAE
HHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCC
>Mature Secondary Structure 
TVRMDKRFADLRAENRPALITYFMGGDPDFQTSLGIMKALPEAGADVIELGMPFSDPMA
CEECCHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
DGPAIQLAGQRALKGGQTLKTTLDLAREFRKQDNATPIVMMGYYNPIYIYGVEKFLDDAI
CCCEEEECCHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEHHHHHHHHH
ASGIDGLIVVDLPPEMDDELCIPALARGINFIRLATPTTDGKRLPAVLKNTSGFVYYVSM
HCCCCCEEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCHHHHCCCCCEEEEEEE
NGITGSALPDPSLISGAVGRIKAHTELPVCVGFGVKTADHAKAIGAVADGVVVGSAIVNQ
CCCCCCCCCCCHHHHHHHHHEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
IAGSLTKDGQATADTVPAVTTLVKGLSTGVRASRLAAAE
HHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194