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The map label for this gene is cobD [H]

Identifier: 15890056

GI number: 15890056

Start: 2812384

End: 2813349

Strand: Reverse

Name: cobD [H]

Synonym: Atu2809

Alternate gene names: 15890056

Gene position: 2813349-2812384 (Counterclockwise)

Preceding gene: 159185392

Following gene: 159185390

Centisome position: 99.01

GC content: 61.59

Gene sequence:

>966_bases
ATGTTCTTTGCTCTCGCTTTCCTGTCGCTCGTGATCGAACGCCTGACCGGCTATCCGGACTGGCTGTTCAAACGCATCGG
CCACCCCGTCACCTGGATCGGTTCGCTTATCGCGCTCATGGACAAAAAATGGAACCGGGAGAGCGCATCATTTTCTCAGC
GCAAAGCCGCAGGTGTTGCGGCGCTGGTTGTTTTTGTCGGGCTAACGGTGATCCTTGCATGGTTTGCGCAGTCGGTTCTG
CTTTTCCTGCCCTTCGGTCTCTTGGCTGTGGCGATATTCGGTGCGTCGCTTCCGGCGCAACAAAGTCTGGAACAGCATGT
GGAGGCAGTCGCCACGGCGCTGGAGCGCGAGGGGGTGGAAGGCGGCCGCAAGGCGGTGTCGATGATCGTCGGGCGCGATC
CGCAAAAGCTAGATGAGGCAGCGATCTGCCGGGCGGCGATCGAAAGTCTCGCCGAGAATTTTTCCGATGGCATCGTCGCT
CCTGCCTTCTGGCTCGGATTTCTGGGCCTGCCTGGTGGTGCGGCTTACAAGGCGATCAATACGGCAGACAGCATGATCGG
TCATCGTTCGCCCCGCCATGAGGCCTTCGGCTGGGCCTCGGCCCGGCTGGATGATCTCGTTAATCTACCGGCCTCAAGGC
TGAGCGGCAGCCTGTTTGTGGTCGCCGCTTTTTTTGTCGAAGGCGCGTCGCCAAAAGCGGCCATTGCCGCGATCCGTCGC
GATGCGCGCCACCACCGCTCCCCCAATGCCGGCTGGCCGGAGGCGGCGCTTGCCGGTGCTCTCGGTTTTGCGCTTGCCGG
CCCGCGTTCCTATGGCGGGCAGATGATCGAGGCGCGTTTCATGGGCGAAGGCGGGAGGTCCGCACTGGTTGCCGGTGATA
TTCGCAAGGCATTGCGACTGGCGCGGATCGCGGACTTGTTGCTGATTGGCCTGTTCGGACTTCTGGCGATCCTTATCGCG
CTATAG

Upstream 100 bases:

>100_bases
GATCCACGGTTCAGGTGCTTGCCGAAACATCAGGCGCGCCTTGCCGAGATTGCCGCCGTGACGGATCGCTCCCTGAGCTT
TTTCTGGTACTGTCTCGCCC

Downstream 100 bases:

>100_bases
CAAGCAGCCTGTCGAGATCGAGGTGCTGTTCCATATGCGCAGCCAGACGGTCGAGAACCGCATCAACCTGCGCGTCATAG
TTCAGGTCCGTGCCCTGCCC

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MFFALAFLSLVIERLTGYPDWLFKRIGHPVTWIGSLIALMDKKWNRESASFSQRKAAGVAALVVFVGLTVILAWFAQSVL
LFLPFGLLAVAIFGASLPAQQSLEQHVEAVATALEREGVEGGRKAVSMIVGRDPQKLDEAAICRAAIESLAENFSDGIVA
PAFWLGFLGLPGGAAYKAINTADSMIGHRSPRHEAFGWASARLDDLVNLPASRLSGSLFVVAAFFVEGASPKAAIAAIRR
DARHHRSPNAGWPEAALAGALGFALAGPRSYGGQMIEARFMGEGGRSALVAGDIRKALRLARIADLLLIGLFGLLAILIA
L

Sequences:

>Translated_321_residues
MFFALAFLSLVIERLTGYPDWLFKRIGHPVTWIGSLIALMDKKWNRESASFSQRKAAGVAALVVFVGLTVILAWFAQSVL
LFLPFGLLAVAIFGASLPAQQSLEQHVEAVATALEREGVEGGRKAVSMIVGRDPQKLDEAAICRAAIESLAENFSDGIVA
PAFWLGFLGLPGGAAYKAINTADSMIGHRSPRHEAFGWASARLDDLVNLPASRLSGSLFVVAAFFVEGASPKAAIAAIRR
DARHHRSPNAGWPEAALAGALGFALAGPRSYGGQMIEARFMGEGGRSALVAGDIRKALRLARIADLLLIGLFGLLAILIA
L
>Mature_321_residues
MFFALAFLSLVIERLTGYPDWLFKRIGHPVTWIGSLIALMDKKWNRESASFSQRKAAGVAALVVFVGLTVILAWFAQSVL
LFLPFGLLAVAIFGASLPAQQSLEQHVEAVATALEREGVEGGRKAVSMIVGRDPQKLDEAAICRAAIESLAENFSDGIVA
PAFWLGFLGLPGGAAYKAINTADSMIGHRSPRHEAFGWASARLDDLVNLPASRLSGSLFVVAAFFVEGASPKAAIAAIRR
DARHHRSPNAGWPEAALAGALGFALAGPRSYGGQMIEARFMGEGGRSALVAGDIRKALRLARIADLLLIGLFGLLAILIA
L

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 34129; Mature: 34129

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFALAFLSLVIERLTGYPDWLFKRIGHPVTWIGSLIALMDKKWNRESASFSQRKAAGVA
CHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ALVVFVGLTVILAWFAQSVLLFLPFGLLAVAIFGASLPAQQSLEQHVEAVATALEREGVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
GGRKAVSMIVGRDPQKLDEAAICRAAIESLAENFSDGIVAPAFWLGFLGLPGGAAYKAIN
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHH
TADSMIGHRSPRHEAFGWASARLDDLVNLPASRLSGSLFVVAAFFVEGASPKAAIAAIRR
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHCCCCCHHHHHHHHH
DARHHRSPNAGWPEAALAGALGFALAGPRSYGGQMIEARFMGEGGRSALVAGDIRKALRL
HHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHH
ARIADLLLIGLFGLLAILIAL
HHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MFFALAFLSLVIERLTGYPDWLFKRIGHPVTWIGSLIALMDKKWNRESASFSQRKAAGVA
CHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ALVVFVGLTVILAWFAQSVLLFLPFGLLAVAIFGASLPAQQSLEQHVEAVATALEREGVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
GGRKAVSMIVGRDPQKLDEAAICRAAIESLAENFSDGIVAPAFWLGFLGLPGGAAYKAIN
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHH
TADSMIGHRSPRHEAFGWASARLDDLVNLPASRLSGSLFVVAAFFVEGASPKAAIAAIRR
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHCCCCCHHHHHHHHH
DARHHRSPNAGWPEAALAGALGFALAGPRSYGGQMIEARFMGEGGRSALVAGDIRKALRL
HHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHH
ARIADLLLIGLFGLLAILIAL
HHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2211520 [H]