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The map label for this gene is braE [H]

Identifier: 15889696

GI number: 15889696

Start: 2397884

End: 2399281

Strand: Reverse

Name: braE [H]

Synonym: Atu2426

Alternate gene names: 15889696

Gene position: 2399281-2397884 (Counterclockwise)

Preceding gene: 15889697

Following gene: 159185210

Centisome position: 84.43

GC content: 58.37

Gene sequence:

>1398_bases
ATGACCAATATCGCTTCCGAAAATGCCGCTTCCAGCCCGAGCCTGATGGCGACGGCCATGAAGGAGGGCCTGATTGCCGG
CGTCATCTCGTTCGGCATGTTCATTCTCTATATCGGCATCATCACCTATCAGGATATCAATAACCAGCTGATCTGGGGCA
CACGCTGGGGGCTGCTGGCGATCTTCGTCGCCGTTGCCGCCGTTGGCCGCTTTCTTGTGGTCGGTTTCATCAAGCCGTCG
CTGGATCGCCGCAAGCTCTCCAAGGCCAAGAGCGGCGTGCTGGAAATCTCCGAAGAAAAAGGCTTTTTTCACAAGCACTT
CCTGAAATTCGCGCTGGTTCTGCTGCTGCTTTATCCCGTGATTGCCGTGCAGCTTTTCGGTTTTCAGGGTTCGCTGAAAT
TCGTCGACAATTTCGGCATCCAGATCCTGATCTACGTGATGCTGGCCTGGGGCCTCAATATCGTCGTCGGTCTCGCTGGC
CTGCTCGATCTCGGTTATGTGGCCTTTTATGCGGTGGGCGCTTATTCCTACGCGCTGCTTTCCAGCCAGTTCGGCCTGTC
CTTCTGGGTGCTTCTGCCGGTTGCCGGTATTCTTGCCGGCTTCTGGGGCGTCATCCTCGGTTTCCCGGTGCTTCGTCTTC
GCGGCGACTATCTGGCGATCGTCACGCTTGCCTTCGGTGAAATCATCCGTCTCGTGCTGTTGAACTGGACGGCCGTCACC
AAGGGCACCTTTGGTATTTCCGGCATCGCCAAGGCGTCCGTCTTCGGCATCTGGTCCTTCGATGTCGGCGCGCCCAACAA
CTTCGCCAAGGTCTTTGGCCTGTCGATGTCGTCGGCCTATTACAAGATTTTCCTGTTCTACGTGATCCTGCTTCTGTGCA
TGCTGACGGCTTACGTCACCATCAAGTTGCGCCGCATGCCGATCGGCCGCGCATGGGAAGCGTTGCGTGAAGATGAAATC
GCCTGCCGTTCGCTCGGCATCGATACCGTCATCACCAAGCTCACCGCCTTTGCCACGGGCGCGATGTTCGGCGGTTTCGC
CGGTTCGTTCTTCGCTGCGCGTCAGGGTTTCGTGTCGCCGGAAAGCTTCGTGTTCCTCGAATCCGCCGTCATTCTCGCCA
TCGTCGTTCTCGGCGGCATGGGCTCGCTGACGGGTATCGCCATTGCGGCGCTCGTCATGGTCGGCGGCACCGAGCTTCTG
CGCGAAATGGAGTTCCTGAAGCATGTCTTCGGGCCTGATTTCACGCCGGAACTTTACCGCATGCTGCTCTTCGGTCTCGC
CATGGTCATCGTCATGCTGTTCAAGCCGCGCGGCTTCGTCGGCTCGCGTGAACCCACGGCCTTCCTCAGGGAACGCAAGG
CCGTGTCCGGCAGCTTTACCAAGGAAGGTCACGGTTGA

Upstream 100 bases:

>100_bases
GCCTACAAGGATGTTGCGGCATTCGGCATCCTGGCATTCGTGCTGATCTTCAAGCCGACCGGTATTCTCGGTCGTCCGGA
AGTCGAGAAGGTGTGATCCC

Downstream 100 bases:

>100_bases
TGGCTTCCGGAACGATGAACATGACATCCAATACGACCGGCAATACAACTGGCGACACGATCCTCAAGGTCGAACATCTC
TCGATGAAGTTCGGCGGCCT

Product: ABC transporter, membrane spanning protein (branched chain amino acid)

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MTNIASENAASSPSLMATAMKEGLIAGVISFGMFILYIGIITYQDINNQLIWGTRWGLLAIFVAVAAVGRFLVVGFIKPS
LDRRKLSKAKSGVLEISEEKGFFHKHFLKFALVLLLLYPVIAVQLFGFQGSLKFVDNFGIQILIYVMLAWGLNIVVGLAG
LLDLGYVAFYAVGAYSYALLSSQFGLSFWVLLPVAGILAGFWGVILGFPVLRLRGDYLAIVTLAFGEIIRLVLLNWTAVT
KGTFGISGIAKASVFGIWSFDVGAPNNFAKVFGLSMSSAYYKIFLFYVILLLCMLTAYVTIKLRRMPIGRAWEALREDEI
ACRSLGIDTVITKLTAFATGAMFGGFAGSFFAARQGFVSPESFVFLESAVILAIVVLGGMGSLTGIAIAALVMVGGTELL
REMEFLKHVFGPDFTPELYRMLLFGLAMVIVMLFKPRGFVGSREPTAFLRERKAVSGSFTKEGHG

Sequences:

>Translated_465_residues
MTNIASENAASSPSLMATAMKEGLIAGVISFGMFILYIGIITYQDINNQLIWGTRWGLLAIFVAVAAVGRFLVVGFIKPS
LDRRKLSKAKSGVLEISEEKGFFHKHFLKFALVLLLLYPVIAVQLFGFQGSLKFVDNFGIQILIYVMLAWGLNIVVGLAG
LLDLGYVAFYAVGAYSYALLSSQFGLSFWVLLPVAGILAGFWGVILGFPVLRLRGDYLAIVTLAFGEIIRLVLLNWTAVT
KGTFGISGIAKASVFGIWSFDVGAPNNFAKVFGLSMSSAYYKIFLFYVILLLCMLTAYVTIKLRRMPIGRAWEALREDEI
ACRSLGIDTVITKLTAFATGAMFGGFAGSFFAARQGFVSPESFVFLESAVILAIVVLGGMGSLTGIAIAALVMVGGTELL
REMEFLKHVFGPDFTPELYRMLLFGLAMVIVMLFKPRGFVGSREPTAFLRERKAVSGSFTKEGHG
>Mature_464_residues
TNIASENAASSPSLMATAMKEGLIAGVISFGMFILYIGIITYQDINNQLIWGTRWGLLAIFVAVAAVGRFLVVGFIKPSL
DRRKLSKAKSGVLEISEEKGFFHKHFLKFALVLLLLYPVIAVQLFGFQGSLKFVDNFGIQILIYVMLAWGLNIVVGLAGL
LDLGYVAFYAVGAYSYALLSSQFGLSFWVLLPVAGILAGFWGVILGFPVLRLRGDYLAIVTLAFGEIIRLVLLNWTAVTK
GTFGISGIAKASVFGIWSFDVGAPNNFAKVFGLSMSSAYYKIFLFYVILLLCMLTAYVTIKLRRMPIGRAWEALREDEIA
CRSLGIDTVITKLTAFATGAMFGGFAGSFFAARQGFVSPESFVFLESAVILAIVVLGGMGSLTGIAIAALVMVGGTELLR
EMEFLKHVFGPDFTPELYRMLLFGLAMVIVMLFKPRGFVGSREPTAFLRERKAVSGSFTKEGHG

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=315, Percent_Identity=52.6984126984127, Blast_Score=281, Evalue=8e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 50626; Mature: 50495

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TNIASENAASSPSLMATAMKEGLIAGVISFGMFILYIGIITYQDINNQLIWGTRWGLLA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHH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HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]