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The map label for this gene is trmL [H]

Identifier: 15889520

GI number: 15889520

Start: 2217210

End: 2217695

Strand: Direct

Name: trmL [H]

Synonym: Atu2244

Alternate gene names: 15889520

Gene position: 2217210-2217695 (Clockwise)

Preceding gene: 159185117

Following gene: 159185120

Centisome position: 78.03

GC content: 62.96

Gene sequence:

>486_bases
ATGCCCGACACTCTGCCTGAAACAGGGCCCAACATCAGGATCGCCCTCTACCAGCCCGATATTCCCGGCAATACCGGCAC
CATCCTACGGCTGGCCGCCTGTCTTGGCCTCGGCGTCGACATCATCGAGCCTGCCGGCTTCGATATTTCCGACCGCAATC
TCAAACGGGCGGGCATGGATTATATTGCGGCGGCGGCTTTGACGCGCCATGTCAGCTGGGATCGTTTCGAGGAGTGGCGG
GCCAGCACCGGTCGCCGCCTCGTTCTCGCCTCCACCAGGGCCGCGCTGCCCTATACCGACATCACCTATCGCGCCGATGA
CATCCTGCTGTTTGGCCGGGAAAGTGCCGGCGTGCCTGATCATGTGCATGACAAGGCGGATGAGCGAATCATCATCCCGA
TGGTGGCCGGACAGCGCTCGATCAACGTCGCCATGTCGGCGGCGATGGTCACTGGCGAAGCCCTGCGCCAGACGGCATGG
TCGTGA

Upstream 100 bases:

>100_bases
CGACCAACGGTCTTAAAAAGGCACTTTTCCCAGCCGCATCTGTCACAGGCTATTCACCCGTGATAATCGGTCCGACAGTC
CCGAATGCGAAGAGAAAACC

Downstream 100 bases:

>100_bases
CCTGCCATCTTTGCAGCCAATTTTTCAGGCAAATATGAAAATTGACCGCTTTTACCGCGAAACTGTCGCAACCCGGCCTG
TACGGTGAGCATTCACGCTC

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 161; Mature: 160

Protein sequence:

>161_residues
MPDTLPETGPNIRIALYQPDIPGNTGTILRLAACLGLGVDIIEPAGFDISDRNLKRAGMDYIAAAALTRHVSWDRFEEWR
ASTGRRLVLASTRAALPYTDITYRADDILLFGRESAGVPDHVHDKADERIIIPMVAGQRSINVAMSAAMVTGEALRQTAW
S

Sequences:

>Translated_161_residues
MPDTLPETGPNIRIALYQPDIPGNTGTILRLAACLGLGVDIIEPAGFDISDRNLKRAGMDYIAAAALTRHVSWDRFEEWR
ASTGRRLVLASTRAALPYTDITYRADDILLFGRESAGVPDHVHDKADERIIIPMVAGQRSINVAMSAAMVTGEALRQTAW
S
>Mature_160_residues
PDTLPETGPNIRIALYQPDIPGNTGTILRLAACLGLGVDIIEPAGFDISDRNLKRAGMDYIAAAALTRHVSWDRFEEWRA
STGRRLVLASTRAALPYTDITYRADDILLFGRESAGVPDHVHDKADERIIIPMVAGQRSINVAMSAAMVTGEALRQTAWS

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=151, Percent_Identity=39.7350993377483, Blast_Score=112, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17574; Mature: 17443

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDTLPETGPNIRIALYQPDIPGNTGTILRLAACLGLGVDIIEPAGFDISDRNLKRAGMD
CCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHH
YIAAAALTRHVSWDRFEEWRASTGRRLVLASTRAALPYTDITYRADDILLFGRESAGVPD
HHHHHHHHHHCCHHHHHHHHHCCCCEEEEEECCCCCCCCCEEECCCEEEEECCCCCCCCC
HVHDKADERIIIPMVAGQRSINVAMSAAMVTGEALRQTAWS
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PDTLPETGPNIRIALYQPDIPGNTGTILRLAACLGLGVDIIEPAGFDISDRNLKRAGMD
CCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHH
YIAAAALTRHVSWDRFEEWRASTGRRLVLASTRAALPYTDITYRADDILLFGRESAGVPD
HHHHHHHHHHCCHHHHHHHHHCCCCEEEEEECCCCCCCCCEEECCCEEEEECCCCCCCCC
HVHDKADERIIIPMVAGQRSINVAMSAAMVTGEALRQTAWS
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA