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The map label for this gene is ycdT [H]

Identifier: 15889298

GI number: 15889298

Start: 1970408

End: 1972147

Strand: Direct

Name: ycdT [H]

Synonym: Atu2010

Alternate gene names: 15889298

Gene position: 1970408-1972147 (Clockwise)

Preceding gene: 159185437

Following gene: 15889304

Centisome position: 69.34

GC content: 59.2

Gene sequence:

>1740_bases
ATGCGCGCGAGCATACAGAAAATCCAGCTGACAGGCACAATCGTTATTGCCGTAAGCGCCATCCTGCTGGCTACACTTGC
CGCGCTGCCCAGCGTTTCCAACTATCTTCGTTATCGAACGAACGCACAGCAACTGAACCGCTTTGAAACCGCGCTGCAAT
CCGCATGGCTGGTCTCGGCCGAGCGCGGCCCGGCCAATAACCTGATGGCGGCCACTTCTGCCGATGCAAGCCTGGTCGAG
CGTCTGGCCGTCGCGCGCAAGGCGACAGACGACAAGTTGTCTCAGGTGGAGACCTCCTTCGCCGAAGAAATCAAAGCGCA
GCCCCGCCTGGCGAAGTCACTTGCCGAAACCCGCCAGAAACTCGCGCAATCGCGAGAGGCCGTAGACGCGGTTGCCGCTC
TTCCCGCGGAAGCGCGCAGTTACAGCGCCATGGCCAACGCCATTCAATCGATGTTCACGGCAGCCGATAGCGTCAACCTG
CTCCGCGGCAAGGCCGCCCGCGCCATCATCACGAAAACACCAGATGTCGCGATCGATATCGCAATGACCGGCACCGCCGG
TGTCATGCGCGACAGGATCGGTCGCCTCGGCTCCTACGTGGTGATGAGCCTGCAGGCGAACAGGCAGGACAGATTAAGTT
ATCGCGCGAAGTTCGACACCGAGATGCAGAGCGTCATGCTGGTGAAATCCTCGCTGTGGAACTACACCGCCGCCTATCTG
TCGACACCGCGTCTTGACGCCGCGTTTCGCGATTTCGACACCTATTATTTCGGCCAGTCGCTTCGCTACGCGCAGAATAC
GATTGCCATCGTCGTACCCTCGGCACTTCCAAGCATCCGCGATTTTTCCGAAAACTACATTCAGGGCATGCGATCGTCAG
ATCAGCTGCGTGACCTTATCCTCGCCGAAACCCGCGCGAGAATCGAAAAGAACAAGCAACAGGCGCTTATCTATGGCCTG
GCCTCGCTCTTTCTCGCCTTGATCGCCGTTATGGTCCTGCTGCGCCTCACGTCAGTCTATAAAACGGCGCTGTTCCAGCC
CATGCAGTCTGTTCTGGCGCAAATCTCCGCGATAGCGGCTGGCGATCTTTCCGAGGCTCAGCGCCAGACCGGCGTTGCCC
CGGAAGTGACGAAGATGTTTCAGGAATTGGATTTCCTGCGCGAGCAGCTTCGGCAGAAGGGAGAAATGGAGGAACAGCAG
CGCGCACTGTCAGAACAGCTCCGCACGCTGTCCGAAACCGATGCGCTGACCGGCGTCTTCAATCGCCGCGCGCTGGAAAA
GTCCGTTCGAGCCATTCTGAGCGGCGATGAGGACTATCAATCCCTCGGCGTCATGATCATCGATATCGATCACTTCAAGT
CGATCAATGACCGTTATGGCCACGCCATGGGCGACCTTGCGCTGCAAAAGACGGCAAGCCTTTTAAAGGGCGCCTTGCGC
AAGACCGATATTCTGGCCCGCTACGGCGGCGAGGAATTCGTCGTCGTATTGCAGGATGTCAGCCACGCCACGGCGACCGC
CACCGCCGACCGCCTTCGCCGCCTGATAGAAGTTCAGACCTTCGATGAACAAACAGGTTTGTCACTGACGGCAAGTTTCG
GCGTCGTCTGGCAGGAGGCGCGCGCAATCAACAGCTGGGACGAGTTGATCGCCATCGCCGACGACCGGCTTTACGAAGCC
AAGCGCGCGGGCCGCAACCGGGTGTGGGCAACCTATTTTCCGAAGGTCGCGAACGGCTAG

Upstream 100 bases:

>100_bases
CGCTATTAGAGCAAAAGGCCCATAAAATAAGCGAAGTGTTTACGCGGCGAAGCTAACATGGTGACAGACCATAACCATTC
CAGCGAGAATAACGCACCCG

Downstream 100 bases:

>100_bases
ACGGCACCGCCGGTCACAAATTCCGCCGCGCCTAGATCAGGTCCAGCGTCATCACGACAGGGCAATGGTCGGACGCCTTC
GGGCGATCCCAGCCGGTGCG

Product: GGDEF family protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MRASIQKIQLTGTIVIAVSAILLATLAALPSVSNYLRYRTNAQQLNRFETALQSAWLVSAERGPANNLMAATSADASLVE
RLAVARKATDDKLSQVETSFAEEIKAQPRLAKSLAETRQKLAQSREAVDAVAALPAEARSYSAMANAIQSMFTAADSVNL
LRGKAARAIITKTPDVAIDIAMTGTAGVMRDRIGRLGSYVVMSLQANRQDRLSYRAKFDTEMQSVMLVKSSLWNYTAAYL
STPRLDAAFRDFDTYYFGQSLRYAQNTIAIVVPSALPSIRDFSENYIQGMRSSDQLRDLILAETRARIEKNKQQALIYGL
ASLFLALIAVMVLLRLTSVYKTALFQPMQSVLAQISAIAAGDLSEAQRQTGVAPEVTKMFQELDFLREQLRQKGEMEEQQ
RALSEQLRTLSETDALTGVFNRRALEKSVRAILSGDEDYQSLGVMIIDIDHFKSINDRYGHAMGDLALQKTASLLKGALR
KTDILARYGGEEFVVVLQDVSHATATATADRLRRLIEVQTFDEQTGLSLTASFGVVWQEARAINSWDELIAIADDRLYEA
KRAGRNRVWATYFPKVANG

Sequences:

>Translated_579_residues
MRASIQKIQLTGTIVIAVSAILLATLAALPSVSNYLRYRTNAQQLNRFETALQSAWLVSAERGPANNLMAATSADASLVE
RLAVARKATDDKLSQVETSFAEEIKAQPRLAKSLAETRQKLAQSREAVDAVAALPAEARSYSAMANAIQSMFTAADSVNL
LRGKAARAIITKTPDVAIDIAMTGTAGVMRDRIGRLGSYVVMSLQANRQDRLSYRAKFDTEMQSVMLVKSSLWNYTAAYL
STPRLDAAFRDFDTYYFGQSLRYAQNTIAIVVPSALPSIRDFSENYIQGMRSSDQLRDLILAETRARIEKNKQQALIYGL
ASLFLALIAVMVLLRLTSVYKTALFQPMQSVLAQISAIAAGDLSEAQRQTGVAPEVTKMFQELDFLREQLRQKGEMEEQQ
RALSEQLRTLSETDALTGVFNRRALEKSVRAILSGDEDYQSLGVMIIDIDHFKSINDRYGHAMGDLALQKTASLLKGALR
KTDILARYGGEEFVVVLQDVSHATATATADRLRRLIEVQTFDEQTGLSLTASFGVVWQEARAINSWDELIAIADDRLYEA
KRAGRNRVWATYFPKVANG
>Mature_579_residues
MRASIQKIQLTGTIVIAVSAILLATLAALPSVSNYLRYRTNAQQLNRFETALQSAWLVSAERGPANNLMAATSADASLVE
RLAVARKATDDKLSQVETSFAEEIKAQPRLAKSLAETRQKLAQSREAVDAVAALPAEARSYSAMANAIQSMFTAADSVNL
LRGKAARAIITKTPDVAIDIAMTGTAGVMRDRIGRLGSYVVMSLQANRQDRLSYRAKFDTEMQSVMLVKSSLWNYTAAYL
STPRLDAAFRDFDTYYFGQSLRYAQNTIAIVVPSALPSIRDFSENYIQGMRSSDQLRDLILAETRARIEKNKQQALIYGL
ASLFLALIAVMVLLRLTSVYKTALFQPMQSVLAQISAIAAGDLSEAQRQTGVAPEVTKMFQELDFLREQLRQKGEMEEQQ
RALSEQLRTLSETDALTGVFNRRALEKSVRAILSGDEDYQSLGVMIIDIDHFKSINDRYGHAMGDLALQKTASLLKGALR
KTDILARYGGEEFVVVLQDVSHATATATADRLRRLIEVQTFDEQTGLSLTASFGVVWQEARAINSWDELIAIADDRLYEA
KRAGRNRVWATYFPKVANG

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=37.1951219512195, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87082007, Length=161, Percent_Identity=40.3726708074534, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI87081881, Length=177, Percent_Identity=39.5480225988701, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI145693134, Length=156, Percent_Identity=37.8205128205128, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1787802, Length=171, Percent_Identity=35.0877192982456, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1786584, Length=174, Percent_Identity=33.3333333333333, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=34.5911949685535, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1788381, Length=181, Percent_Identity=33.7016574585635, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787541, Length=191, Percent_Identity=31.9371727748691, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788085, Length=173, Percent_Identity=33.5260115606936, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI87081977, Length=231, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1788956, Length=178, Percent_Identity=32.0224719101124, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI87081974, Length=121, Percent_Identity=32.2314049586777, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1787056, Length=159, Percent_Identity=31.4465408805031, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 64014; Mature: 64014

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS50906 NIT ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRASIQKIQLTGTIVIAVSAILLATLAALPSVSNYLRYRTNAQQLNRFETALQSAWLVSA
CCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEC
ERGPANNLMAATSADASLVERLAVARKATDDKLSQVETSFAEEIKAQPRLAKSLAETRQK
CCCCCCCCEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LAQSREAVDAVAALPAEARSYSAMANAIQSMFTAADSVNLLRGKAARAIITKTPDVAIDI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEE
AMTGTAGVMRDRIGRLGSYVVMSLQANRQDRLSYRAKFDTEMQSVMLVKSSLWNYTAAYL
EECCCHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STPRLDAAFRDFDTYYFGQSLRYAQNTIAIVVPSALPSIRDFSENYIQGMRSSDQLRDLI
CCCCHHHHHHHHHHHHHCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHH
LAETRARIEKNKQQALIYGLASLFLALIAVMVLLRLTSVYKTALFQPMQSVLAQISAIAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GDLSEAQRQTGVAPEVTKMFQELDFLREQLRQKGEMEEQQRALSEQLRTLSETDALTGVF
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
NRRALEKSVRAILSGDEDYQSLGVMIIDIDHFKSINDRYGHAMGDLALQKTASLLKGALR
HHHHHHHHHHHHHCCCCCHHHCCEEEEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
KTDILARYGGEEFVVVLQDVSHATATATADRLRRLIEVQTFDEQTGLSLTASFGVVWQEA
HHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHH
RAINSWDELIAIADDRLYEAKRAGRNRVWATYFPKVANG
HHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MRASIQKIQLTGTIVIAVSAILLATLAALPSVSNYLRYRTNAQQLNRFETALQSAWLVSA
CCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEC
ERGPANNLMAATSADASLVERLAVARKATDDKLSQVETSFAEEIKAQPRLAKSLAETRQK
CCCCCCCCEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LAQSREAVDAVAALPAEARSYSAMANAIQSMFTAADSVNLLRGKAARAIITKTPDVAIDI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEE
AMTGTAGVMRDRIGRLGSYVVMSLQANRQDRLSYRAKFDTEMQSVMLVKSSLWNYTAAYL
EECCCHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STPRLDAAFRDFDTYYFGQSLRYAQNTIAIVVPSALPSIRDFSENYIQGMRSSDQLRDLI
CCCCHHHHHHHHHHHHHCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHH
LAETRARIEKNKQQALIYGLASLFLALIAVMVLLRLTSVYKTALFQPMQSVLAQISAIAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GDLSEAQRQTGVAPEVTKMFQELDFLREQLRQKGEMEEQQRALSEQLRTLSETDALTGVF
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
NRRALEKSVRAILSGDEDYQSLGVMIIDIDHFKSINDRYGHAMGDLALQKTASLLKGALR
HHHHHHHHHHHHHCCCCCHHHCCEEEEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
KTDILARYGGEEFVVVLQDVSHATATATADRLRRLIEVQTFDEQTGLSLTASFGVVWQEA
HHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHH
RAINSWDELIAIADDRLYEAKRAGRNRVWATYFPKVANG
HHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]