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The map label for this gene is glnA [H]

Identifier: 15889073

GI number: 15889073

Start: 1754245

End: 1755654

Strand: Direct

Name: glnA [H]

Synonym: Atu1770

Alternate gene names: 15889073

Gene position: 1754245-1755654 (Clockwise)

Preceding gene: 159184909

Following gene: 15889074

Centisome position: 61.73

GC content: 59.08

Gene sequence:

>1410_bases
ATGACGACCGCAAACGATATCTTGAAGCAGATCAAGGATAACGACATCAAGTTCGTGGACCTGCGCTTTACCGATCCCAA
GGGTAAGCTGCAGCACGTCACCATGGATGTTGTCTGTGTGGACGAAGACATGTTCGCCGATGGCGTCATGTTCGACGGCT
CCTCGATCGCCGGCTGGAAGGCCATCAACGAGTCCGACATGGTGCTGATGCCCGATCCGGCCACCGCCCACATCGACCCG
TTCTTTGCCCAGTCTACCCTGGTCGTCCTTTGCGACATTCTCGACCCGGTTTCGGGCGAAGCCTATAACCGCGACCCGCG
CGGCACCGCGAAGAAAGCCGAAGCCTACCTCAAGGCCTCTGGTATCGGCGACACCGTTTTCGTCGGCCCCGAGCCGGAAT
TCTTCGTCTTCGACGACGTGAAGTACAAGGCAGACCCCTACAACACCGGCTTCAAGCTTGATTCGTCCGAACTGCCGTCC
AACGACGACACGGATTACGAAACCGGCAACCTCGGCCACCGTCCGCGCGTCAAGGGCGGCTACTTCCCGGTTCCGCCGAT
CGACAGCCTGCAGGACATGCGTTCTGAAATGCTGACGGTTCTGGCCGAAATGGGCGTCGTCGTTGAAAAGCACCACCACG
AAGTGGCTGCCGCACAGCACGAGCTGGGCGTGAAGTTCGACACGCTGGTTTCCAGCGCCGACAAGATGCAGATCTACAAG
TACGTCGTGCACCAGGTCGCCAATGCCTATGGCAAGACGGCAACCTTCATGCCGAAGCCGATCTTCGGCGATAATGGCTC
GGGCATGCACGTTCACCAGTCGATCTGGAAGGGCGGCAAGCCGACCTTCGCTGGCGACGAATATGCTGGCCTGTCCGAAA
ATTGCCTCTACTACATCGGCGGCATCATCAAGCATGCCAAGGCAATCAACGCCTTCACCAACCCTACCACCAACTCCTAC
AAGCGTCTGGTGCCGGGTTACGAAGCGCCGGTTCTGCTCGCCTACTCCGCGCGCAACCGCTCCGCATCGTGCCGTATTCC
CTTCGGCTCCAACCCGAAGGCAAAGCGCGTCGAAGTCCGCTTCCCGGACCCGACCCAGAACCCCTATCTCGGTTTTGCGG
CCATGCTGATGGCCGGCCTCGACGGCATCAAGAACAAGATCCACCCCGGCAAGCCGATGGACAAGGACCTTTACGATCTT
CCTGCCAAGGAACTGAAGAAGATCCCGACCGTCTGCGGCTCGCTGCGTGAAGCGCTGGAAAGCCTCGACAAGGATCGCAA
GTTCCTGACCGCCGGCGGCGTGTTCGACGACGACCAGATCGACTCCTTCATCGAACTGAAGATGCAGGAAGTCATGCGTT
TCGAAATGACCCCGCATCCGGTCGAATTCGACATGTACTACTCGGCCTGA

Upstream 100 bases:

>100_bases
CGGTGAAACAGGCGTGGACGCCATATAATCAGAACCAGGTCTCAAGCCGGGGAGGCTTGGGTTTCACCTCGTCATCATCA
CACTCAAGGAAAACGTTTAA

Downstream 100 bases:

>100_bases
TCTAACTTCGGAGCGCTCCCTTGGGGCGCTCCTTCCATGCCGGCGGTTTTCCCTGGAAAGCCGCCGTTTTCTTTTGAAGC
CCAGCCATATCGTGAAGAAT

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase I; Glutamine synthetase I; GSI [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTTANDILKQIKDNDIKFVDLRFTDPKGKLQHVTMDVVCVDEDMFADGVMFDGSSIAGWKAINESDMVLMPDPATAHIDP
FFAQSTLVVLCDILDPVSGEAYNRDPRGTAKKAEAYLKASGIGDTVFVGPEPEFFVFDDVKYKADPYNTGFKLDSSELPS
NDDTDYETGNLGHRPRVKGGYFPVPPIDSLQDMRSEMLTVLAEMGVVVEKHHHEVAAAQHELGVKFDTLVSSADKMQIYK
YVVHQVANAYGKTATFMPKPIFGDNGSGMHVHQSIWKGGKPTFAGDEYAGLSENCLYYIGGIIKHAKAINAFTNPTTNSY
KRLVPGYEAPVLLAYSARNRSASCRIPFGSNPKAKRVEVRFPDPTQNPYLGFAAMLMAGLDGIKNKIHPGKPMDKDLYDL
PAKELKKIPTVCGSLREALESLDKDRKFLTAGGVFDDDQIDSFIELKMQEVMRFEMTPHPVEFDMYYSA

Sequences:

>Translated_469_residues
MTTANDILKQIKDNDIKFVDLRFTDPKGKLQHVTMDVVCVDEDMFADGVMFDGSSIAGWKAINESDMVLMPDPATAHIDP
FFAQSTLVVLCDILDPVSGEAYNRDPRGTAKKAEAYLKASGIGDTVFVGPEPEFFVFDDVKYKADPYNTGFKLDSSELPS
NDDTDYETGNLGHRPRVKGGYFPVPPIDSLQDMRSEMLTVLAEMGVVVEKHHHEVAAAQHELGVKFDTLVSSADKMQIYK
YVVHQVANAYGKTATFMPKPIFGDNGSGMHVHQSIWKGGKPTFAGDEYAGLSENCLYYIGGIIKHAKAINAFTNPTTNSY
KRLVPGYEAPVLLAYSARNRSASCRIPFGSNPKAKRVEVRFPDPTQNPYLGFAAMLMAGLDGIKNKIHPGKPMDKDLYDL
PAKELKKIPTVCGSLREALESLDKDRKFLTAGGVFDDDQIDSFIELKMQEVMRFEMTPHPVEFDMYYSA
>Mature_468_residues
TTANDILKQIKDNDIKFVDLRFTDPKGKLQHVTMDVVCVDEDMFADGVMFDGSSIAGWKAINESDMVLMPDPATAHIDPF
FAQSTLVVLCDILDPVSGEAYNRDPRGTAKKAEAYLKASGIGDTVFVGPEPEFFVFDDVKYKADPYNTGFKLDSSELPSN
DDTDYETGNLGHRPRVKGGYFPVPPIDSLQDMRSEMLTVLAEMGVVVEKHHHEVAAAQHELGVKFDTLVSSADKMQIYKY
VVHQVANAYGKTATFMPKPIFGDNGSGMHVHQSIWKGGKPTFAGDEYAGLSENCLYYIGGIIKHAKAINAFTNPTTNSYK
RLVPGYEAPVLLAYSARNRSASCRIPFGSNPKAKRVEVRFPDPTQNPYLGFAAMLMAGLDGIKNKIHPGKPMDKDLYDLP
AKELKKIPTVCGSLREALESLDKDRKFLTAGGVFDDDQIDSFIELKMQEVMRFEMTPHPVEFDMYYSA

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=467, Percent_Identity=65.3104925053533, Blast_Score=646, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=394, Percent_Identity=25.6345177664975, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 51992; Mature: 51860

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTANDILKQIKDNDIKFVDLRFTDPKGKLQHVTMDVVCVDEDMFADGVMFDGSSIAGWK
CCCHHHHHHHHCCCCEEEEEEEEECCCCCEEEEEEEEEEECCCCCCCCEEECCCCCCCCE
AINESDMVLMPDPATAHIDPFFAQSTLVVLCDILDPVSGEAYNRDPRGTAKKAEAYLKAS
ECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCC
GIGDTVFVGPEPEFFVFDDVKYKADPYNTGFKLDSSELPSNDDTDYETGNLGHRPRVKGG
CCCCEEEECCCCCEEEEECCEEECCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCC
YFPVPPIDSLQDMRSEMLTVLAEMGVVVEKHHHEVAAAQHELGVKFDTLVSSADKMQIYK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHH
YVVHQVANAYGKTATFMPKPIFGDNGSGMHVHQSIWKGGKPTFAGDEYAGLSENCLYYIG
HHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
GIIKHAKAINAFTNPTTNSYKRLVPGYEAPVLLAYSARNRSASCRIPFGSNPKAKRVEVR
HHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCEEEEE
FPDPTQNPYLGFAAMLMAGLDGIKNKIHPGKPMDKDLYDLPAKELKKIPTVCGSLREALE
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHH
SLDKDRKFLTAGGVFDDDQIDSFIELKMQEVMRFEMTPHPVEFDMYYSA
HHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECC
>Mature Secondary Structure 
TTANDILKQIKDNDIKFVDLRFTDPKGKLQHVTMDVVCVDEDMFADGVMFDGSSIAGWK
CCHHHHHHHHCCCCEEEEEEEEECCCCCEEEEEEEEEEECCCCCCCCEEECCCCCCCCE
AINESDMVLMPDPATAHIDPFFAQSTLVVLCDILDPVSGEAYNRDPRGTAKKAEAYLKAS
ECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCC
GIGDTVFVGPEPEFFVFDDVKYKADPYNTGFKLDSSELPSNDDTDYETGNLGHRPRVKGG
CCCCEEEECCCCCEEEEECCEEECCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCC
YFPVPPIDSLQDMRSEMLTVLAEMGVVVEKHHHEVAAAQHELGVKFDTLVSSADKMQIYK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHH
YVVHQVANAYGKTATFMPKPIFGDNGSGMHVHQSIWKGGKPTFAGDEYAGLSENCLYYIG
HHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
GIIKHAKAINAFTNPTTNSYKRLVPGYEAPVLLAYSARNRSASCRIPFGSNPKAKRVEVR
HHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCEEEEE
FPDPTQNPYLGFAAMLMAGLDGIKNKIHPGKPMDKDLYDLPAKELKKIPTVCGSLREALE
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHH
SLDKDRKFLTAGGVFDDDQIDSFIELKMQEVMRFEMTPHPVEFDMYYSA
HHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2882467 [H]