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The map label for this gene is tgt

Identifier: 15888983

GI number: 15888983

Start: 1664545

End: 1665675

Strand: Reverse

Name: tgt

Synonym: Atu1677

Alternate gene names: 15888983

Gene position: 1665675-1664545 (Counterclockwise)

Preceding gene: 159184871

Following gene: 159184870

Centisome position: 58.62

GC content: 63.22

Gene sequence:

>1131_bases
ATGCACGAGAAATTCACCTTCACCCTGAAATCCACGAGCGGCGGCGCGCGCCTTGGCGAAGTCGCCATGCCGCGCGGTGT
CATCCGCACGCCCGCCTTCATGCCGGTTGGCACCGTTGGTACCGTCAAGGCCATGTATCTCGACCAGGTGCGCGAGCTGG
GGGCCGATATCATTCTCGGCAATACCTATCACCTGATGCTGCGGCCCGGCCCGGAGCGCGTTGCCCGTCTCGGCGGTCTG
CACGAGCTGATCCGCTGGCCGCACCCGATCCTCACCGACAGCGGCGGCTTTCAGGTGATGTCGCTTTCCGGCCTGCGCAA
GCTCGACGAGAAGGGCGTGACCTTCAAGAGCCATGTGGACGGTTCGCTGCACCACATGTCGCCGGAACGCTCGATCGAAA
TTCAGGGCATGCTAGATTCGGACATTCAGATGCAGCTTGACGAGTGCATCGCGCTGCCGGCCGAGCGTAAGGAAATCGAG
CGTGCCATGGAAATGTCGCTGCGCTGGGCCGAGCGCTGCCGCGTCGCCTTTGGCGAACAGCCCGGCAAGGCCATGTTCGG
CATCGTCCAGGGCGGCGACCAGCCGGACCTGCGTATCCGTTCCGCCGAAGGGCTGAAAGAACTTGATCTCAAGGGTTATG
CCGTCGGCGGTCTTGCCGTCGGCGAGCCGCAGGATGTGATGCTCGGCATGCTCGATATCACGCTGCCGGTGCTGCCCACC
GAAAAGCCGCGTTACCTGATGGGTGTCGGCACGCCTGACGATATCCTGAAATCGGTGGCGCGCGGCATCGACATGTTCGA
CTGCGTGATGCCGACCCGCTCGGGCCGTCACGGGCTGGCGTTTACCCGCCGTGGCCGCGTCAACATCCGCAACGCCCGTC
ATGCCGAGGATATGCGCCCGCTCGACGAGCAGTCCAACTGCCCGGCCTCGCGCGATTATTCCCGCGCCTATCTGCATCAC
CTCACGCGCTCCAACGAAGCGCTGGGCGGCATGCTGCTCTCCTGGCACAATCTTGCCTATTATCAGGAGCTGATGCAGGG
TATCCGCACGTCGATCGAGGAAGGTCGTTTCGCCGATTTTTATGCGGAAACCATCGAAATGTGGGCGCGGGGCGACATAG
ACCCGGTCTGA

Upstream 100 bases:

>100_bases
GCGGTCTCGAAACGATGCGTGACGCTTACAGGCACGCCATCGAAACCGGATACCGCTTCTATTCCTATGGCGATTCCAGC
CTGTTGTTCCGGAAAAACTG

Downstream 100 bases:

>100_bases
TCTTTTCCGACTCTTCCTCCTGTTGCGGACGCTTTCCTTGGCACATGCGCCTTACGTTCTTTTCCGGGATGACACGACCG
GCACGGTGACAGCCTTCACC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MHEKFTFTLKSTSGGARLGEVAMPRGVIRTPAFMPVGTVGTVKAMYLDQVRELGADIILGNTYHLMLRPGPERVARLGGL
HELIRWPHPILTDSGGFQVMSLSGLRKLDEKGVTFKSHVDGSLHHMSPERSIEIQGMLDSDIQMQLDECIALPAERKEIE
RAMEMSLRWAERCRVAFGEQPGKAMFGIVQGGDQPDLRIRSAEGLKELDLKGYAVGGLAVGEPQDVMLGMLDITLPVLPT
EKPRYLMGVGTPDDILKSVARGIDMFDCVMPTRSGRHGLAFTRRGRVNIRNARHAEDMRPLDEQSNCPASRDYSRAYLHH
LTRSNEALGGMLLSWHNLAYYQELMQGIRTSIEEGRFADFYAETIEMWARGDIDPV

Sequences:

>Translated_376_residues
MHEKFTFTLKSTSGGARLGEVAMPRGVIRTPAFMPVGTVGTVKAMYLDQVRELGADIILGNTYHLMLRPGPERVARLGGL
HELIRWPHPILTDSGGFQVMSLSGLRKLDEKGVTFKSHVDGSLHHMSPERSIEIQGMLDSDIQMQLDECIALPAERKEIE
RAMEMSLRWAERCRVAFGEQPGKAMFGIVQGGDQPDLRIRSAEGLKELDLKGYAVGGLAVGEPQDVMLGMLDITLPVLPT
EKPRYLMGVGTPDDILKSVARGIDMFDCVMPTRSGRHGLAFTRRGRVNIRNARHAEDMRPLDEQSNCPASRDYSRAYLHH
LTRSNEALGGMLLSWHNLAYYQELMQGIRTSIEEGRFADFYAETIEMWARGDIDPV
>Mature_376_residues
MHEKFTFTLKSTSGGARLGEVAMPRGVIRTPAFMPVGTVGTVKAMYLDQVRELGADIILGNTYHLMLRPGPERVARLGGL
HELIRWPHPILTDSGGFQVMSLSGLRKLDEKGVTFKSHVDGSLHHMSPERSIEIQGMLDSDIQMQLDECIALPAERKEIE
RAMEMSLRWAERCRVAFGEQPGKAMFGIVQGGDQPDLRIRSAEGLKELDLKGYAVGGLAVGEPQDVMLGMLDITLPVLPT
EKPRYLMGVGTPDDILKSVARGIDMFDCVMPTRSGRHGLAFTRRGRVNIRNARHAEDMRPLDEQSNCPASRDYSRAYLHH
LTRSNEALGGMLLSWHNLAYYQELMQGIRTSIEEGRFADFYAETIEMWARGDIDPV

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=345, Percent_Identity=44.9275362318841, Blast_Score=315, Evalue=6e-86,
Organism=Homo sapiens, GI13375872, Length=267, Percent_Identity=24.7191011235955, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1786607, Length=365, Percent_Identity=52.3287671232877, Blast_Score=374, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17544680, Length=360, Percent_Identity=40.5555555555556, Blast_Score=277, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI25146502, Length=166, Percent_Identity=29.5180722891566, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19920510, Length=359, Percent_Identity=44.0111420612813, Blast_Score=318, Evalue=4e-87,
Organism=Drosophila melanogaster, GI21355311, Length=246, Percent_Identity=26.0162601626016, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_AGRT5 (Q8UES8)

Other databases:

- EMBL:   AE007869
- PIR:   AG2782
- PIR:   H97561
- RefSeq:   NP_354664.1
- ProteinModelPortal:   Q8UES8
- SMR:   Q8UES8
- STRING:   Q8UES8
- GeneID:   1133715
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu1677
- eggNOG:   COG0343
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- PhylomeDB:   Q8UES8
- ProtClustDB:   PRK00112
- BioCyc:   ATUM176299-1:ATU1677-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 41946; Mature: 41946

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: ACT_SITE 93-93 BINDING 94-94

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHEKFTFTLKSTSGGARLGEVAMPRGVIRTPAFMPVGTVGTVKAMYLDQVRELGADIILG
CCCEEEEEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEC
NTYHLMLRPGPERVARLGGLHELIRWPHPILTDSGGFQVMSLSGLRKLDEKGVTFKSHVD
CEEEEEECCCHHHHHHHCCHHHHHHCCCCEEECCCCEEEEEHHHHHHHHHCCCEEEECCC
GSLHHMSPERSIEIQGMLDSDIQMQLDECIALPAERKEIERAMEMSLRWAERCRVAFGEQ
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
PGKAMFGIVQGGDQPDLRIRSAEGLKELDLKGYAVGGLAVGEPQDVMLGMLDITLPVLPT
CCCEEEEEECCCCCCCEEEECCCCCHHHCCCCEEECCEECCCCHHHHHHHHHHEEECCCC
EKPRYLMGVGTPDDILKSVARGIDMFDCVMPTRSGRHGLAFTRRGRVNIRNARHAEDMRP
CCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEECCCEEECCCCCHHHCCC
LDEQSNCPASRDYSRAYLHHLTRSNEALGGMLLSWHNLAYYQELMQGIRTSIEEGRFADF
CCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
YAETIEMWARGDIDPV
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MHEKFTFTLKSTSGGARLGEVAMPRGVIRTPAFMPVGTVGTVKAMYLDQVRELGADIILG
CCCEEEEEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEC
NTYHLMLRPGPERVARLGGLHELIRWPHPILTDSGGFQVMSLSGLRKLDEKGVTFKSHVD
CEEEEEECCCHHHHHHHCCHHHHHHCCCCEEECCCCEEEEEHHHHHHHHHCCCEEEECCC
GSLHHMSPERSIEIQGMLDSDIQMQLDECIALPAERKEIERAMEMSLRWAERCRVAFGEQ
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
PGKAMFGIVQGGDQPDLRIRSAEGLKELDLKGYAVGGLAVGEPQDVMLGMLDITLPVLPT
CCCEEEEEECCCCCCCEEEECCCCCHHHCCCCEEECCEECCCCHHHHHHHHHHEEECCCC
EKPRYLMGVGTPDDILKSVARGIDMFDCVMPTRSGRHGLAFTRRGRVNIRNARHAEDMRP
CCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEECCCEEECCCCCHHHCCC
LDEQSNCPASRDYSRAYLHHLTRSNEALGGMLLSWHNLAYYQELMQGIRTSIEEGRFADF
CCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
YAETIEMWARGDIDPV
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194