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The map label for this gene is ycjG [H]

Identifier: 15888958

GI number: 15888958

Start: 1631826

End: 1632809

Strand: Direct

Name: ycjG [H]

Synonym: Atu1648

Alternate gene names: 15888958

Gene position: 1631826-1632809 (Clockwise)

Preceding gene: 15888953

Following gene: 159184856

Centisome position: 57.43

GC content: 62.3

Gene sequence:

>984_bases
ATGCCCCGTTACCTTCAAGCCACAACTGAACGTTTCGCCGTTGCTGGCAGCTTCACCATTTCACGCGGCACCCGGACGCA
TGCGGATGTCGTAACGTGCACCATCCGCGATGGTTCCTTCACCGGAATTGGCGAATGTGTGCCCTATCCGCGTTATGGCG
AAAGCATCGAAGGCGTCACCGCCGATATCGAAGCGATGGCGGATCGAGTAGCAGCCGGCCTGACACGGCAGGAATTGCAG
CAGGTGATGAAACCGGGGGCCGCCCGCAATGCGGTCGATTGCGCGCTCTGGGACCTCGAAGCGAAGATGAGCGGCAAGAG
AGCCGCCGAACAGGTTCTCGGACAGCCGGCACAACCGCTCGTCACCGCCTATACGATCTCGCTCGCCGACCCGGACACCA
TGGCCGCAAAGACCGCGGAAAATGCCGGCCGCCCGCTCCTGAAGATCAAGACCGGCACCGCCGATGACGAAGCCCGCCTG
CGAGCCGTGCGCGCCGCGGCACCCGAGGCGCGTATCATCATCGATGCGAACGAAGGCTGGAACGACGACAATATCGAATA
TTATCTGAAGCTTGCGGCGGAACTGAAGATATCGCTGATCGAACAGCCCCTGCCCGCCGGCAAGGATGCCATGCTCGCCC
GCATCGAACACCCGGTTCTGATCTGCGCCGATGAAAGCGTGCATTCCACCGAAGACCTCGCCGGCCTTCGCGACCGTTAC
GATGCAATCAACATCAAGCTCGACAAGACCGGTGGCCTGACGGAAGCGCTCGTCATGAAGGCGGAAGCCGAACGGCTCGG
TTTCACCATCATGGTCGGCTGCATGCTCGGCACCTCGCTGGGAATGGCCCCCGCCGTGCTGGTCGCCCAGGGCACGGCCT
TTGCCGATCTCGACGGGCCGCTGTTGTTGGCTGAGGATCGTGATCCGGGGCTGGTTTATGAGGGTTCGCTGGTTTATCCG
GCGCGGCCGGAACTGTGGGGGTAA

Upstream 100 bases:

>100_bases
TCATAACGGTTTGGCCTTCGGCAAGCCACAGTGACGGCCGACAATTCGCGATAAATTTGGCGTCATGTGTCTTTTGACGC
CTATTAGGAAGTGAAAAAAC

Downstream 100 bases:

>100_bases
GCCCTGAAATTAAAAAAAGATGAGGTGCTACGCCGCCGCGTATTTCTTCTCCCCGCCGGGGAGAAGGTGGCCCGAAGGGT
CGGATGAGGGGGCACCGTTG

Product: mandelate racemase/muconate lactonizing enzyme family protein

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MPRYLQATTERFAVAGSFTISRGTRTHADVVTCTIRDGSFTGIGECVPYPRYGESIEGVTADIEAMADRVAAGLTRQELQ
QVMKPGAARNAVDCALWDLEAKMSGKRAAEQVLGQPAQPLVTAYTISLADPDTMAAKTAENAGRPLLKIKTGTADDEARL
RAVRAAAPEARIIIDANEGWNDDNIEYYLKLAAELKISLIEQPLPAGKDAMLARIEHPVLICADESVHSTEDLAGLRDRY
DAINIKLDKTGGLTEALVMKAEAERLGFTIMVGCMLGTSLGMAPAVLVAQGTAFADLDGPLLLAEDRDPGLVYEGSLVYP
ARPELWG

Sequences:

>Translated_327_residues
MPRYLQATTERFAVAGSFTISRGTRTHADVVTCTIRDGSFTGIGECVPYPRYGESIEGVTADIEAMADRVAAGLTRQELQ
QVMKPGAARNAVDCALWDLEAKMSGKRAAEQVLGQPAQPLVTAYTISLADPDTMAAKTAENAGRPLLKIKTGTADDEARL
RAVRAAAPEARIIIDANEGWNDDNIEYYLKLAAELKISLIEQPLPAGKDAMLARIEHPVLICADESVHSTEDLAGLRDRY
DAINIKLDKTGGLTEALVMKAEAERLGFTIMVGCMLGTSLGMAPAVLVAQGTAFADLDGPLLLAEDRDPGLVYEGSLVYP
ARPELWG
>Mature_326_residues
PRYLQATTERFAVAGSFTISRGTRTHADVVTCTIRDGSFTGIGECVPYPRYGESIEGVTADIEAMADRVAAGLTRQELQQ
VMKPGAARNAVDCALWDLEAKMSGKRAAEQVLGQPAQPLVTAYTISLADPDTMAAKTAENAGRPLLKIKTGTADDEARLR
AVRAAAPEARIIIDANEGWNDDNIEYYLKLAAELKISLIEQPLPAGKDAMLARIEHPVLICADESVHSTEDLAGLRDRYD
AINIKLDKTGGLTEALVMKAEAERLGFTIMVGCMLGTSLGMAPAVLVAQGTAFADLDGPLLLAEDRDPGLVYEGSLVYPA
RPELWG

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, excep

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=313, Percent_Identity=43.7699680511182, Blast_Score=258, Evalue=3e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 35046; Mature: 34915

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRYLQATTERFAVAGSFTISRGTRTHADVVTCTIRDGSFTGIGECVPYPRYGESIEGVT
CCCHHHHHHHHEEEEEEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCH
ADIEAMADRVAAGLTRQELQQVMKPGAARNAVDCALWDLEAKMSGKRAAEQVLGQPAQPL
HHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEEEHHHHHCHHHHHHHHHCCCCCCE
VTAYTISLADPDTMAAKTAENAGRPLLKIKTGTADDEARLRAVRAAAPEARIIIDANEGW
EEEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCC
NDDNIEYYLKLAAELKISLIEQPLPAGKDAMLARIEHPVLICADESVHSTEDLAGLRDRY
CCCCCEEEEEEHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCC
DAINIKLDKTGGLTEALVMKAEAERLGFTIMVGCMLGTSLGMAPAVLVAQGTAFADLDGP
EEEEEEEECCCCHHHHHHHHHHHHHCCHHEEEHHHHHCCCCCCCEEEEECCCEEEECCCC
LLLAEDRDPGLVYEGSLVYPARPELWG
EEEECCCCCCEEEECCEECCCCCCCCC
>Mature Secondary Structure 
PRYLQATTERFAVAGSFTISRGTRTHADVVTCTIRDGSFTGIGECVPYPRYGESIEGVT
CCHHHHHHHHEEEEEEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCH
ADIEAMADRVAAGLTRQELQQVMKPGAARNAVDCALWDLEAKMSGKRAAEQVLGQPAQPL
HHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEEEHHHHHCHHHHHHHHHCCCCCCE
VTAYTISLADPDTMAAKTAENAGRPLLKIKTGTADDEARLRAVRAAAPEARIIIDANEGW
EEEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCC
NDDNIEYYLKLAAELKISLIEQPLPAGKDAMLARIEHPVLICADESVHSTEDLAGLRDRY
CCCCCEEEEEEHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCC
DAINIKLDKTGGLTEALVMKAEAERLGFTIMVGCMLGTSLGMAPAVLVAQGTAFADLDGP
EEEEEEEECCCCHHHHHHHHHHHHHCCHHEEEHHHHHCCCCCCCEEEEECCCEEEECCCC
LLLAEDRDPGLVYEGSLVYPARPELWG
EEEECCCCCCEEEECCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7499381; 9097039; 9278503 [H]