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The map label for this gene is maeB [H]

Identifier: 159184856

GI number: 159184856

Start: 1635918

End: 1638233

Strand: Direct

Name: maeB [H]

Synonym: Atu1652

Alternate gene names: 159184856

Gene position: 1635918-1638233 (Clockwise)

Preceding gene: 15888958

Following gene: 159184857

Centisome position: 57.57

GC content: 62.31

Gene sequence:

>2316_bases
ATGACCTCTCACGATAAGAACAACACGCCCGCCAACACGGCAAAGGCCGACATCGAGGAACAGGCGCTCTTTTTCCACCG
CTACCCACGCCCCGGCAAGCTGGAAATCCAGGCCACCAAGCCACTCGGCAACCAGCGGGATCTGGCGCTTGCCTATTCGC
CGGGTGTTGCCGCCCCCTGCCTCGCCATCCATGAGAACCCGGAAATGGCAGCGGAATATACCGCGCGCGCCAATCTCGTC
GCCGTCATCTCAAACGGCACGGCGGTGCTCGGCCTCGGCAATATCGGTCCGCTCGCTTCCAAGCCTGTTATGGAAGGCAA
GGCCGTCCTCTTCAAGAAATTCGCCGGTATCGACGTGTTCGATATCGAGATCGATGCGCCCGGCATCAACGACATGGTCT
CCACCATCGCCGCGCTGGAACCCACCTTCGGCGGCATCAACCTTGAGGATATCAAGGCGCCGGAATGTTTCGAGGTGGAA
CGCCAGCTGCGCGAAAAGATGAACATCCCGGTCTTCCACGATGACCAGCACGGCACGGCGATCATCGTCGCCGCCGCCGT
CACCAACGCGCTGGAACTGGCCGGAAAATCGCTCTCGAGCGTCAAGATCGTTGCGTCCGGTGCCGGTGCGGCAGCGCTTG
CCTGCCTCAACCTGCTGGTCGCCATGGGTGCAAACAAGGAAAACATCTGGGTCCACGATATCGAAGGCCTCGTTTATGAA
GGCCGCAACACGCTGATGGACGAGTGGAAAGAAGTTTACGCCCAGAAGACAGACAAGCGTGTGCTGGCGGATTCGATCGA
CGGTGCCGATGTCTTCCTCGGCCTTTCCGCCGCGGGCGTGCTGAAGCCGGAACTCTTGGAGCGCATGGCGGAAAATCCGC
TGATTCTCGCGCTTGCCAACCCCAATCCCGAAATCATGCCGGAGGCTGCCCGTGCCGCCCGCCCGGATGCGATGATTTGC
ACCGGCCGTTCGGATTTCCCGAACCAGGTCAACAATGTTCTCTGCTTCCCCTACATCTTCCGCGGCGCGCTGGATTGCGG
CGCAACCACGATCAACGAGGAAATGAAGATGGCCGCCGTGCAGGCCATTGCAGAGCTTGCCCGCGAGGAAGTCTCGGAAG
TCGCCGCCAGAGCCTATAGCGGCGAAACGCCGATCTTTGGCCCGAACTATCTCATTCCCTCGCCGTTCGATCCGCGCCTC
ATCCTGCGCATTGCACCCGCCGTTGCCCGCGCTGCGGCGGCAAGCGGCGTGGCCACCCGGCCGATCACCGATTTCGAGGC
CTATTTCGACCAGCTGAACCGTTTCGTCTGGCGCTCCGGCTTCATCATGAAGCCGGTCTTCAATGCCGCCAAGGCGGCCG
AAAAGAAGCGCATCATCTTTGCCGAAGGCGAAGACGAGCGCGTGCTGCGCGCCGCCCAGGTGCTTTTGGAAGAAGGCACC
GGCGTCCCGATCCTCATCGGTCGCCCGCAGATCATCGAAACGCGCCTGAAGCGCTTCGGCCTGCGCATCCGCCCGCATGC
GGATTTCGCCGTGGTCAACCCGGAAGACGATCCGCGCTACCGCGAATATGTGGATGATTATTTCGCCCTCGTCGGTCGCG
CCGGCATCAACCCGGAAGCCGCCCGCACCATCGTGCGTACCAACTCCACCGTCATCGGTGCGCTGTCGGTGAAGCGTGGC
GAAGCGGATGCGCTGATCTGTGGTCTGGAAGGCCGTTATGACCGCCATCTGCGCGACGTGAACCAGATCATCGGCAAGCA
GGAAAGTGTGCGCTCCTTTGCGGGCCTCAGCCTTCTCATTACCCAGCAGGGCGCCCTGTTCCTGACCGATACCTTCGTCA
ACAACGACCCGACGTCAGAAGAAGTGGCCGAGATGGCGATCCTTGCGGCGAAGGAAATCCGTCGTTTCGGCATTACGCCG
AAGATCGCGCTCGCCAGCCATTCCAACTTCGGATCGCGTGACTCGGAAAGCGCCCGCAAGATGCGCCGCGCACTGAAGAT
CATCCAGCAGGCAGCGCCAGAACTGGAAGTGGATGGTGAGATGCAGGGCGGCTCGGCACTCTCCGAAGCGCTGCGCAAGC
GTGCCATGCCCAACAGCGTGCTGACCGGCGAGGCGAACCTTCTGGTCTTCCCGAACCTCGATGCCGCCAATATCACGCTC
GGTGTCACTCGCACGCTGACGGAAGGCCTGCATGTCGGCCCGATCCTGCTCGGCACGGCCCTGCCCGCCCACATCCTGTC
GCCCAGCGTCACCTCGCGCGGCGTCGTCAACATGGCGGCCTTTGCCGTGGTGCAGGCCTCGCACCCTTCGGTGTGA

Upstream 100 bases:

>100_bases
GCCTGCATTTGCATGAGAAGGCGGTAAATAACGGCTGTACCCTGGGGAAGTTTGTTGTATTCAGGGGGCCGGAATTGAAC
CAGATATGACGGTGGAAGAC

Downstream 100 bases:

>100_bases
TGAAGGCGCATTAACGCAGTTTTGAACATGCTGTTGCCGCAATGCGGCAACAGCATATGTTATCTTCCCGAAACACGGAT
TTCCTGAAGCGTAACACCCC

Product: malic enzyme

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 771; Mature: 770

Protein sequence:

>771_residues
MTSHDKNNTPANTAKADIEEQALFFHRYPRPGKLEIQATKPLGNQRDLALAYSPGVAAPCLAIHENPEMAAEYTARANLV
AVISNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEIDAPGINDMVSTIAALEPTFGGINLEDIKAPECFEVE
RQLREKMNIPVFHDDQHGTAIIVAAAVTNALELAGKSLSSVKIVASGAGAAALACLNLLVAMGANKENIWVHDIEGLVYE
GRNTLMDEWKEVYAQKTDKRVLADSIDGADVFLGLSAAGVLKPELLERMAENPLILALANPNPEIMPEAARAARPDAMIC
TGRSDFPNQVNNVLCFPYIFRGALDCGATTINEEMKMAAVQAIAELAREEVSEVAARAYSGETPIFGPNYLIPSPFDPRL
ILRIAPAVARAAAASGVATRPITDFEAYFDQLNRFVWRSGFIMKPVFNAAKAAEKKRIIFAEGEDERVLRAAQVLLEEGT
GVPILIGRPQIIETRLKRFGLRIRPHADFAVVNPEDDPRYREYVDDYFALVGRAGINPEAARTIVRTNSTVIGALSVKRG
EADALICGLEGRYDRHLRDVNQIIGKQESVRSFAGLSLLITQQGALFLTDTFVNNDPTSEEVAEMAILAAKEIRRFGITP
KIALASHSNFGSRDSESARKMRRALKIIQQAAPELEVDGEMQGGSALSEALRKRAMPNSVLTGEANLLVFPNLDAANITL
GVTRTLTEGLHVGPILLGTALPAHILSPSVTSRGVVNMAAFAVVQASHPSV

Sequences:

>Translated_771_residues
MTSHDKNNTPANTAKADIEEQALFFHRYPRPGKLEIQATKPLGNQRDLALAYSPGVAAPCLAIHENPEMAAEYTARANLV
AVISNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEIDAPGINDMVSTIAALEPTFGGINLEDIKAPECFEVE
RQLREKMNIPVFHDDQHGTAIIVAAAVTNALELAGKSLSSVKIVASGAGAAALACLNLLVAMGANKENIWVHDIEGLVYE
GRNTLMDEWKEVYAQKTDKRVLADSIDGADVFLGLSAAGVLKPELLERMAENPLILALANPNPEIMPEAARAARPDAMIC
TGRSDFPNQVNNVLCFPYIFRGALDCGATTINEEMKMAAVQAIAELAREEVSEVAARAYSGETPIFGPNYLIPSPFDPRL
ILRIAPAVARAAAASGVATRPITDFEAYFDQLNRFVWRSGFIMKPVFNAAKAAEKKRIIFAEGEDERVLRAAQVLLEEGT
GVPILIGRPQIIETRLKRFGLRIRPHADFAVVNPEDDPRYREYVDDYFALVGRAGINPEAARTIVRTNSTVIGALSVKRG
EADALICGLEGRYDRHLRDVNQIIGKQESVRSFAGLSLLITQQGALFLTDTFVNNDPTSEEVAEMAILAAKEIRRFGITP
KIALASHSNFGSRDSESARKMRRALKIIQQAAPELEVDGEMQGGSALSEALRKRAMPNSVLTGEANLLVFPNLDAANITL
GVTRTLTEGLHVGPILLGTALPAHILSPSVTSRGVVNMAAFAVVQASHPSV
>Mature_770_residues
TSHDKNNTPANTAKADIEEQALFFHRYPRPGKLEIQATKPLGNQRDLALAYSPGVAAPCLAIHENPEMAAEYTARANLVA
VISNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEIDAPGINDMVSTIAALEPTFGGINLEDIKAPECFEVER
QLREKMNIPVFHDDQHGTAIIVAAAVTNALELAGKSLSSVKIVASGAGAAALACLNLLVAMGANKENIWVHDIEGLVYEG
RNTLMDEWKEVYAQKTDKRVLADSIDGADVFLGLSAAGVLKPELLERMAENPLILALANPNPEIMPEAARAARPDAMICT
GRSDFPNQVNNVLCFPYIFRGALDCGATTINEEMKMAAVQAIAELAREEVSEVAARAYSGETPIFGPNYLIPSPFDPRLI
LRIAPAVARAAAASGVATRPITDFEAYFDQLNRFVWRSGFIMKPVFNAAKAAEKKRIIFAEGEDERVLRAAQVLLEEGTG
VPILIGRPQIIETRLKRFGLRIRPHADFAVVNPEDDPRYREYVDDYFALVGRAGINPEAARTIVRTNSTVIGALSVKRGE
ADALICGLEGRYDRHLRDVNQIIGKQESVRSFAGLSLLITQQGALFLTDTFVNNDPTSEEVAEMAILAAKEIRRFGITPK
IALASHSNFGSRDSESARKMRRALKIIQQAAPELEVDGEMQGGSALSEALRKRAMPNSVLTGEANLLVFPNLDAANITLG
VTRTLTEGLHVGPILLGTALPAHILSPSVTSRGVVNMAAFAVVQASHPSV

Specific function: Required for symbiotic nitrogen fixation. Plays a key role in the conversion of malate to acetyl-CoA for efficient tricarboxylic acid cycle function in nitrogen-fixating bacteria [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505145, Length=354, Percent_Identity=25.7062146892655, Blast_Score=83, Evalue=9e-16,
Organism=Homo sapiens, GI62420882, Length=410, Percent_Identity=25.1219512195122, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI62420880, Length=410, Percent_Identity=25.1219512195122, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI239049447, Length=410, Percent_Identity=25.1219512195122, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI4505143, Length=398, Percent_Identity=23.1155778894472, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI270265879, Length=318, Percent_Identity=26.4150943396226, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788806, Length=752, Percent_Identity=57.7127659574468, Blast_Score=897, Evalue=0.0,
Organism=Escherichia coli, GI1788800, Length=313, Percent_Identity=30.9904153354633, Blast_Score=132, Evalue=1e-31,
Organism=Escherichia coli, GI1788635, Length=314, Percent_Identity=28.0254777070064, Blast_Score=125, Evalue=8e-30,
Organism=Escherichia coli, GI87081919, Length=317, Percent_Identity=24.9211356466877, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17537199, Length=411, Percent_Identity=24.5742092457421, Blast_Score=67, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322823, Length=239, Percent_Identity=30.1255230125523, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356279, Length=401, Percent_Identity=25.1870324189526, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281362672, Length=401, Percent_Identity=25.1870324189526, Blast_Score=74, Evalue=5e-13,
Organism=Drosophila melanogaster, GI281362674, Length=401, Percent_Identity=25.1870324189526, Blast_Score=74, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24646388, Length=400, Percent_Identity=25.75, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24646386, Length=400, Percent_Identity=25.75, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.39 [H]

Molecular weight: Translated: 83100; Mature: 82969

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSHDKNNTPANTAKADIEEQALFFHRYPRPGKLEIQATKPLGNQRDLALAYSPGVAAPC
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCE
LAIHENPEMAAEYTARANLVAVISNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVF
EEECCCCHHHHHHHCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCEEE
DIEIDAPGINDMVSTIAALEPTFGGINLEDIKAPECFEVERQLREKMNIPVFHDDQHGTA
EEEECCCCHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCEEECCCCCCE
IIVAAAVTNALELAGKSLSSVKIVASGAGAAALACLNLLVAMGANKENIWVHDIEGLVYE
EEEHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCEEEC
GRNTLMDEWKEVYAQKTDKRVLADSIDGADVFLGLSAAGVLKPELLERMAENPLILALAN
CCHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEECCCCCCCCHHHHHHHCCCCEEEEECC
PNPEIMPEAARAARPDAMICTGRSDFPNQVNNVLCFPYIFRGALDCGATTINEEMKMAAV
CCCCCCCHHHHHCCCCCEEEECCCCCCHHHHCEEEHHHHHHHHHHCCCHHHHHHHHHHHH
QAIAELAREEVSEVAARAYSGETPIFGPNYLIPSPFDPRLILRIAPAVARAAAASGVATR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCC
PITDFEAYFDQLNRFVWRSGFIMKPVFNAAKAAEKKRIIFAEGEDERVLRAAQVLLEEGT
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCC
GVPILIGRPQIIETRLKRFGLRIRPHADFAVVNPEDDPRYREYVDDYFALVGRAGINPEA
CCEEEECCCHHHHHHHHHCCCEEECCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHH
ARTIVRTNSTVIGALSVKRGEADALICGLEGRYDRHLRDVNQIIGKQESVRSFAGLSLLI
HHEEEECCCEEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHCCHHHHHHHCCEEEEE
TQQGALFLTDTFVNNDPTSEEVAEMAILAAKEIRRFGITPKIALASHSNFGSRDSESARK
ECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHH
MRRALKIIQQAAPELEVDGEMQGGSALSEALRKRAMPNSVLTGEANLLVFPNLDAANITL
HHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHCCCCCEEECCCCEEEECCCCCCCEEE
GVTRTLTEGLHVGPILLGTALPAHILSPSVTSRGVVNMAAFAVVQASHPSV
EEHHHHHCCCCCCHHHHHHHCHHHHCCCCCCCCCHHHHHHHHEEECCCCCC
>Mature Secondary Structure 
TSHDKNNTPANTAKADIEEQALFFHRYPRPGKLEIQATKPLGNQRDLALAYSPGVAAPC
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCE
LAIHENPEMAAEYTARANLVAVISNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVF
EEECCCCHHHHHHHCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCEEE
DIEIDAPGINDMVSTIAALEPTFGGINLEDIKAPECFEVERQLREKMNIPVFHDDQHGTA
EEEECCCCHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCEEECCCCCCE
IIVAAAVTNALELAGKSLSSVKIVASGAGAAALACLNLLVAMGANKENIWVHDIEGLVYE
EEEHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCEEEC
GRNTLMDEWKEVYAQKTDKRVLADSIDGADVFLGLSAAGVLKPELLERMAENPLILALAN
CCHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEECCCCCCCCHHHHHHHCCCCEEEEECC
PNPEIMPEAARAARPDAMICTGRSDFPNQVNNVLCFPYIFRGALDCGATTINEEMKMAAV
CCCCCCCHHHHHCCCCCEEEECCCCCCHHHHCEEEHHHHHHHHHHCCCHHHHHHHHHHHH
QAIAELAREEVSEVAARAYSGETPIFGPNYLIPSPFDPRLILRIAPAVARAAAASGVATR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCC
PITDFEAYFDQLNRFVWRSGFIMKPVFNAAKAAEKKRIIFAEGEDERVLRAAQVLLEEGT
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCC
GVPILIGRPQIIETRLKRFGLRIRPHADFAVVNPEDDPRYREYVDDYFALVGRAGINPEA
CCEEEECCCHHHHHHHHHCCCEEECCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHH
ARTIVRTNSTVIGALSVKRGEADALICGLEGRYDRHLRDVNQIIGKQESVRSFAGLSLLI
HHEEEECCCEEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHCCHHHHHHHCCEEEEE
TQQGALFLTDTFVNNDPTSEEVAEMAILAAKEIRRFGITPKIALASHSNFGSRDSESARK
ECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHH
MRRALKIIQQAAPELEVDGEMQGGSALSEALRKRAMPNSVLTGEANLLVFPNLDAANITL
HHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHCCCCCEEECCCCEEEECCCCCCCEEE
GVTRTLTEGLHVGPILLGTALPAHILSPSVTSRGVVNMAAFAVVQASHPSV
EEHHHHHCCCCCCHHHHHHHCHHHHCCCCCCCCCHHHHHHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9535928; 11481430; 10407149 [H]