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The map label for this gene is gcvH

Identifier: 15888789

GI number: 15888789

Start: 1460699

End: 1461061

Strand: Reverse

Name: gcvH

Synonym: Atu1463

Alternate gene names: 15888789

Gene position: 1461061-1460699 (Counterclockwise)

Preceding gene: 229007177

Following gene: 15888788

Centisome position: 51.42

GC content: 57.85

Gene sequence:

>363_bases
ATGCTGAAATTTACCGCAGAACACGAATGGCTGAAGTTCGAAGGCGATATCGCCACCGTCGGCATCACCAGCCACGCCGC
TGAACAGCTTGGCGATCTCGTTTTTGTCGAGCTGCCGGAAGTGGGCGCAACCTTCGCCAAGGATGGCGATGCTGCGACCG
TGGAATCCGTCAAGGCGGCGTCCGACGTCTATTGTCCGCTGGATGGCGAAGTGGTCGAAATCAACCAGGCCATCGTTGAT
GACCCCTCGCTGGTCAATTCCGATCCGCAGGGTGCCGGCTGGTTCTTCAAGCTGAAGCTTTCCAATGCCGCGGATGCCGA
GACACTGCTCGATGAAGCAGCCTACAAGGAGCTGATCGCGTAA

Upstream 100 bases:

>100_bases
GAAGTGCGCGGCAAATATCTGCCCGTCACCGTCGCCGCCCTGCCCTTCATCAAGCCCACCTACAAACGCTAATCCTCATT
CCATCTCCGGAGAGAACACG

Downstream 100 bases:

>100_bases
TGACGACGCCCACGGAATTCCATTTCACCGACTATCAGCCCTATGATTTCGCCAACCGGCGTCACATCGGGCCGTCGCCG
TCGGAAATGGCGGAGATGCT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 120; Mature: 120

Protein sequence:

>120_residues
MLKFTAEHEWLKFEGDIATVGITSHAAEQLGDLVFVELPEVGATFAKDGDAATVESVKAASDVYCPLDGEVVEINQAIVD
DPSLVNSDPQGAGWFFKLKLSNAADAETLLDEAAYKELIA

Sequences:

>Translated_120_residues
MLKFTAEHEWLKFEGDIATVGITSHAAEQLGDLVFVELPEVGATFAKDGDAATVESVKAASDVYCPLDGEVVEINQAIVD
DPSLVNSDPQGAGWFFKLKLSNAADAETLLDEAAYKELIA
>Mature_120_residues
MLKFTAEHEWLKFEGDIATVGITSHAAEQLGDLVFVELPEVGATFAKDGDAATVESVKAASDVYCPLDGEVVEINQAIVD
DPSLVNSDPQGAGWFFKLKLSNAADAETLLDEAAYKELIA

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=117, Percent_Identity=47.8632478632479, Blast_Score=117, Evalue=1e-27,
Organism=Homo sapiens, GI89057342, Length=117, Percent_Identity=47.8632478632479, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789271, Length=119, Percent_Identity=56.3025210084034, Blast_Score=138, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17551294, Length=115, Percent_Identity=41.7391304347826, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17507493, Length=112, Percent_Identity=45.5357142857143, Blast_Score=109, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6319272, Length=107, Percent_Identity=43.9252336448598, Blast_Score=103, Evalue=5e-24,
Organism=Drosophila melanogaster, GI17865652, Length=119, Percent_Identity=44.5378151260504, Blast_Score=108, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_AGRT5 (Q8UFD5)

Other databases:

- EMBL:   AE007869
- PIR:   AG2756
- PIR:   F97537
- RefSeq:   NP_354470.1
- ProteinModelPortal:   Q8UFD5
- SMR:   Q8UFD5
- STRING:   Q8UFD5
- GeneID:   1133501
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu1463
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   QGAGWFF
- PhylomeDB:   Q8UFD5
- ProtClustDB:   PRK01202
- BioCyc:   ATUM176299-1:ATU1463-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 12815; Mature: 12815

Theoretical pI: Translated: 3.84; Mature: 3.84

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKFTAEHEWLKFEGDIATVGITSHAAEQLGDLVFVELPEVGATFAKDGDAATVESVKAA
CCEECCCCCEEEEECCEEEEEECHHHHHHHCCEEEEECCCCCCEECCCCCCHHHHHHHCC
SDVYCPLDGEVVEINQAIVDDPSLVNSDPQGAGWFFKLKLSNAADAETLLDEAAYKELIA
CCEECCCCCCEEEECEECCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKFTAEHEWLKFEGDIATVGITSHAAEQLGDLVFVELPEVGATFAKDGDAATVESVKAA
CCEECCCCCEEEEECCEEEEEECHHHHHHHCCEEEEECCCCCCEECCCCCCHHHHHHHCC
SDVYCPLDGEVVEINQAIVDDPSLVNSDPQGAGWFFKLKLSNAADAETLLDEAAYKELIA
CCEECCCCCCEEEECEECCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194