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The map label for this gene is ntrB [H]

Identifier: 15888770

GI number: 15888770

Start: 1438154

End: 1439302

Strand: Direct

Name: ntrB [H]

Synonym: Atu1445

Alternate gene names: 15888770

Gene position: 1438154-1439302 (Clockwise)

Preceding gene: 15888769

Following gene: 15888771

Centisome position: 50.61

GC content: 59.53

Gene sequence:

>1149_bases
ATGAGCGCCGATAAACACAGCCCGGCAGATGCGAACCAGCTCGCCATGGCGGTTCTGAACGCAGTGCAGAACCCGGTCAT
TCTGGTGGATGGCGAAGGTTTCATTTCATTCGCCAATTGGGAAGCCGAAGCCTTTTTCGGGGCGAGCGCCTCCCATCTTG
CCCGTTATCGCGTCTCCACTTTCATTCCCTTCGGCAGCCCGCTTCTGGCGCTGATCGATCAGGTGCGGGAGCGCCGTGCG
CCGGTCAACGAATATCGCGTCGATCTGAGTTCGCCACGCCTCGGTCAGGACAAGCTGGTTGATCTCTACGTTGCGCCCGT
CGTCAGTGAACCGGGGTCGGTTGTCGTCGTCTTCCAGGAACGGTCGATGGCCGACAAGATCGACCGTCAACTGACCCATC
GCGCCGCCGCCCGCTCGGTTACCGGCCTTGCCTCGATGCTGGCGCATGAGATCAAGAATCCGCTGTCCGGTATTCGTGGC
GCGGCCCAGTTGCTGGAAATGTCGGTGCCTGATGAGGACCGCGCCCTTACCAGGCTTATCTGCGACGAGACTGACCGCAT
CGTGTCATTGGTCGATCGTATGGAAATCTTTTCCGACGAGCGGCCGGTCGACCGCGTGCCGGTCAATATCCACTCCGTGC
TTGATCACGTGAAGGCGATTGCCAAGGCCGGTTTTGCCCGCAACATCAAGATATCGGAGAATTATGATCCCTCGCTGCCG
GCGGTCTATGCCAATCGCGATCAACTGGTGCAGGTCTTCCTCAATCTGGTGAAGAATGCAGCCGAGGCAGTCGCCAATCA
GCCGGATGGTGAGGTTGTGCTGACGACCGCCTATCGCCCCGGTATTCGGCTTTCGGTTGCCGGTAGCCGCGAGCGCATCT
CGCTGCCGCTTGAATTCTGCGTGCATGATAATGGACCGGGGGTCCCTTCCGATCTTCTGCCGCATCTCTTCGATCCCTTC
ATCACCACCAAGACCAATGGTTCGGGCCTCGGCCTGGCGCTTGTCGCCAAGCTGATTGGCGCCCATGGCGGCATTGTCGA
ATGCGACAGCCAGAACCACCGCACGACTTTCCGCGTATTGATGCCCGTCTCGCCGGAAGTGGCGCTTGACGACAGCTCTT
TGCCGAACACGACAGGAAATGACAGATGA

Upstream 100 bases:

>100_bases
AGGCTCAAATCATGACATCGCGTGACACGGGGGAGGTGGCGGATCTGCTTCGCTCCGCCCTGAGCGAAAATGCGGGCGAA
GAAGCCGCAAGGAAGGCCGC

Downstream 100 bases:

>100_bases
CAGCTACGATCCTCGTCGCCGATGATGATGCCGCAATCCGCACGGTGCTGAACCAGGCGCTCAGCCGTGCCGGTTATGAC
GTGCGCATCACCTCCAATGC

Product: two component sensor kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MSADKHSPADANQLAMAVLNAVQNPVILVDGEGFISFANWEAEAFFGASASHLARYRVSTFIPFGSPLLALIDQVRERRA
PVNEYRVDLSSPRLGQDKLVDLYVAPVVSEPGSVVVVFQERSMADKIDRQLTHRAAARSVTGLASMLAHEIKNPLSGIRG
AAQLLEMSVPDEDRALTRLICDETDRIVSLVDRMEIFSDERPVDRVPVNIHSVLDHVKAIAKAGFARNIKISENYDPSLP
AVYANRDQLVQVFLNLVKNAAEAVANQPDGEVVLTTAYRPGIRLSVAGSRERISLPLEFCVHDNGPGVPSDLLPHLFDPF
ITTKTNGSGLGLALVAKLIGAHGGIVECDSQNHRTTFRVLMPVSPEVALDDSSLPNTTGNDR

Sequences:

>Translated_382_residues
MSADKHSPADANQLAMAVLNAVQNPVILVDGEGFISFANWEAEAFFGASASHLARYRVSTFIPFGSPLLALIDQVRERRA
PVNEYRVDLSSPRLGQDKLVDLYVAPVVSEPGSVVVVFQERSMADKIDRQLTHRAAARSVTGLASMLAHEIKNPLSGIRG
AAQLLEMSVPDEDRALTRLICDETDRIVSLVDRMEIFSDERPVDRVPVNIHSVLDHVKAIAKAGFARNIKISENYDPSLP
AVYANRDQLVQVFLNLVKNAAEAVANQPDGEVVLTTAYRPGIRLSVAGSRERISLPLEFCVHDNGPGVPSDLLPHLFDPF
ITTKTNGSGLGLALVAKLIGAHGGIVECDSQNHRTTFRVLMPVSPEVALDDSSLPNTTGNDR
>Mature_381_residues
SADKHSPADANQLAMAVLNAVQNPVILVDGEGFISFANWEAEAFFGASASHLARYRVSTFIPFGSPLLALIDQVRERRAP
VNEYRVDLSSPRLGQDKLVDLYVAPVVSEPGSVVVVFQERSMADKIDRQLTHRAAARSVTGLASMLAHEIKNPLSGIRGA
AQLLEMSVPDEDRALTRLICDETDRIVSLVDRMEIFSDERPVDRVPVNIHSVLDHVKAIAKAGFARNIKISENYDPSLPA
VYANRDQLVQVFLNLVKNAAEAVANQPDGEVVLTTAYRPGIRLSVAGSRERISLPLEFCVHDNGPGVPSDLLPHLFDPFI
TTKTNGSGLGLALVAKLIGAHGGIVECDSQNHRTTFRVLMPVSPEVALDDSSLPNTTGNDR

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=361, Percent_Identity=31.8559556786704, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1788549, Length=352, Percent_Identity=26.4204545454545, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1790436, Length=226, Percent_Identity=30.9734513274336, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI48994928, Length=254, Percent_Identity=27.5590551181102, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790567, Length=171, Percent_Identity=31.5789473684211, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1788393, Length=211, Percent_Identity=26.0663507109005, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 41481; Mature: 41349

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADKHSPADANQLAMAVLNAVQNPVILVDGEGFISFANWEAEAFFGASASHLARYRVST
CCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCHHEECCCHHHHHHHHHHH
FIPFGSPLLALIDQVRERRAPVNEYRVDLSSPRLGQDKLVDLYVAPVVSEPGSVVVVFQE
CCCCCCHHHHHHHHHHHHCCCHHHHEEECCCCCCCCCCEEEEEEEEEECCCCCEEEEECC
RSMADKIDRQLTHRAAARSVTGLASMLAHEIKNPLSGIRGAAQLLEMSVPDEDRALTRLI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
CDETDRIVSLVDRMEIFSDERPVDRVPVNIHSVLDHVKAIAKAGFARNIKISENYDPSLP
HCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCC
AVYANRDQLVQVFLNLVKNAAEAVANQPDGEVVLTTAYRPGIRLSVAGSRERISLPLEFC
EEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECCCCCEEECCEEEE
VHDNGPGVPSDLLPHLFDPFITTKTNGSGLGLALVAKLIGAHGGIVECDSQNHRTTFRVL
EECCCCCCCHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCCEEEEEE
MPVSPEVALDDSSLPNTTGNDR
EECCCCEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SADKHSPADANQLAMAVLNAVQNPVILVDGEGFISFANWEAEAFFGASASHLARYRVST
CCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCHHEECCCHHHHHHHHHHH
FIPFGSPLLALIDQVRERRAPVNEYRVDLSSPRLGQDKLVDLYVAPVVSEPGSVVVVFQE
CCCCCCHHHHHHHHHHHHCCCHHHHEEECCCCCCCCCCEEEEEEEEEECCCCCEEEEECC
RSMADKIDRQLTHRAAARSVTGLASMLAHEIKNPLSGIRGAAQLLEMSVPDEDRALTRLI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
CDETDRIVSLVDRMEIFSDERPVDRVPVNIHSVLDHVKAIAKAGFARNIKISENYDPSLP
HCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCC
AVYANRDQLVQVFLNLVKNAAEAVANQPDGEVVLTTAYRPGIRLSVAGSRERISLPLEFC
EEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECCCCCEEECCEEEE
VHDNGPGVPSDLLPHLFDPFITTKTNGSGLGLALVAKLIGAHGGIVECDSQNHRTTFRVL
EECCCCCCCHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCCEEEEEE
MPVSPEVALDDSSLPNTTGNDR
EECCCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 2881918 [H]