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The map label for this gene is nifR [H]

Identifier: 15888769

GI number: 15888769

Start: 1437141

End: 1438157

Strand: Direct

Name: nifR [H]

Synonym: Atu1444

Alternate gene names: 15888769

Gene position: 1437141-1438157 (Clockwise)

Preceding gene: 15888766

Following gene: 15888770

Centisome position: 50.58

GC content: 61.46

Gene sequence:

>1017_bases
ATGAAAGATCATCATTTGCCTCTTCCGGAGTTGTCAGAACCGTTCAGCATCGGCTCAGTCACGATCCGTAATCGTGCGGT
GCTGGCGCCCATGTCAGGTGTGACGGATCTGCCTTTCCGGCAGCTGGCCTGGCGTTACGGCGCAGGTCTGGTCGTGACGG
AAATGGTCGCCAGCCGCGAACTCGTCGCTAACAGGGGCGAATCCTGGGCACGGCTGAAAAATGCCGGCATGGTTCCGCAT
ATGGTGCAGCTTGCCGGGCGCGAAGCGCATTTTATGGCCGAGGCCGCGAAGATTGCTGCTGACAACGGTGCTGGCATCAT
CGACATCAATATGGGTTGTCCGGCCAAGAAGGTGACGGGCGGTTATTCCGGCTCGGCTTTGATGCGCGATCCCGATCATG
CCCTGTCGCTGATCGAGGCGACGGTTGGCGCGGTTGATGTGCCGGTAACGCTGAAGATGCGGCTCGGCTGGGACGAAAAC
AGCATCAACGCGCCGGAAATTGCCCGCCGTGCCGAGGCGGCAGGCGTGCAGCTCATCACCATTCATGGTCGTACCCGCAT
GCAGTTTTATGAAGGACGGGCGGATTGGGACGCCATCCGCGCCGTGCGCGAGGTGATTTCCGTGCCGCTGATCGCCAATG
GTGACGTGGAAACGGCCGAGGATGCGCGCGAAATCCTGCGTCGCTCCGGTGCGGATGCCGTCATGGTCGGCCGCGGCGCG
CAGGGGCAGCCTTGGCTGCCGGCTGTGCTCGCCGGACATTCAGCGCCGCAACGCGCCGATATTCCCACTATCGCCGTTGA
ACATTACGAGATGATGCTGGAATTTTATGGAAGGGAAGCCGGTCTGCGGCATGCCCGTAAACATCTGGGCTGGTATCTCG
ACCGTTCCGCTCCCGACATCGCCACGCCGGAAAAGGCTCAAATCATGACATCGCGTGACACGGGGGAGGTGGCGGATCTG
CTTCGCTCCGCCCTGAGCGAAAATGCGGGCGAAGAAGCCGCAAGGAAGGCCGCATGA

Upstream 100 bases:

>100_bases
ACAGGCAGGCAGCCGCCCAGCAAATAAAAGGTGCAACCCTCTAAAAGTGCTTGGCAACCGCACAAACACTGTCTAAAAAT
AATGCAAATAGCCCTTCTGC

Downstream 100 bases:

>100_bases
GCGCCGATAAACACAGCCCGGCAGATGCGAACCAGCTCGCCATGGCGGTTCTGAACGCAGTGCAGAACCCGGTCATTCTG
GTGGATGGCGAAGGTTTCAT

Product: nitrogen regulation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKDHHLPLPELSEPFSIGSVTIRNRAVLAPMSGVTDLPFRQLAWRYGAGLVVTEMVASRELVANRGESWARLKNAGMVPH
MVQLAGREAHFMAEAAKIAADNGAGIIDINMGCPAKKVTGGYSGSALMRDPDHALSLIEATVGAVDVPVTLKMRLGWDEN
SINAPEIARRAEAAGVQLITIHGRTRMQFYEGRADWDAIRAVREVISVPLIANGDVETAEDAREILRRSGADAVMVGRGA
QGQPWLPAVLAGHSAPQRADIPTIAVEHYEMMLEFYGREAGLRHARKHLGWYLDRSAPDIATPEKAQIMTSRDTGEVADL
LRSALSENAGEEAARKAA

Sequences:

>Translated_338_residues
MKDHHLPLPELSEPFSIGSVTIRNRAVLAPMSGVTDLPFRQLAWRYGAGLVVTEMVASRELVANRGESWARLKNAGMVPH
MVQLAGREAHFMAEAAKIAADNGAGIIDINMGCPAKKVTGGYSGSALMRDPDHALSLIEATVGAVDVPVTLKMRLGWDEN
SINAPEIARRAEAAGVQLITIHGRTRMQFYEGRADWDAIRAVREVISVPLIANGDVETAEDAREILRRSGADAVMVGRGA
QGQPWLPAVLAGHSAPQRADIPTIAVEHYEMMLEFYGREAGLRHARKHLGWYLDRSAPDIATPEKAQIMTSRDTGEVADL
LRSALSENAGEEAARKAA
>Mature_338_residues
MKDHHLPLPELSEPFSIGSVTIRNRAVLAPMSGVTDLPFRQLAWRYGAGLVVTEMVASRELVANRGESWARLKNAGMVPH
MVQLAGREAHFMAEAAKIAADNGAGIIDINMGCPAKKVTGGYSGSALMRDPDHALSLIEATVGAVDVPVTLKMRLGWDEN
SINAPEIARRAEAAGVQLITIHGRTRMQFYEGRADWDAIRAVREVISVPLIANGDVETAEDAREILRRSGADAVMVGRGA
QGQPWLPAVLAGHSAPQRADIPTIAVEHYEMMLEFYGREAGLRHARKHLGWYLDRSAPDIATPEKAQIMTSRDTGEVADL
LRSALSENAGEEAARKAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=231, Percent_Identity=32.034632034632, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI239788462, Length=236, Percent_Identity=32.6271186440678, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI239788483, Length=228, Percent_Identity=33.3333333333333, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI40807366, Length=259, Percent_Identity=32.8185328185328, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI8923374, Length=253, Percent_Identity=29.2490118577075, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1789660, Length=293, Percent_Identity=45.3924914675768, Blast_Score=248, Evalue=5e-67,
Organism=Escherichia coli, GI1788462, Length=307, Percent_Identity=33.2247557003257, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI145693211, Length=233, Percent_Identity=27.0386266094421, Blast_Score=72, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17543114, Length=298, Percent_Identity=30.5369127516779, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI25144369, Length=268, Percent_Identity=31.7164179104478, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17507177, Length=264, Percent_Identity=31.0606060606061, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17510279, Length=244, Percent_Identity=27.4590163934426, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323560, Length=276, Percent_Identity=30.0724637681159, Blast_Score=113, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6323437, Length=237, Percent_Identity=32.0675105485232, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6323433, Length=308, Percent_Identity=26.9480519480519, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24580595, Length=236, Percent_Identity=33.8983050847458, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI19920448, Length=236, Percent_Identity=33.8983050847458, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24585320, Length=329, Percent_Identity=31.0030395136778, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI45549423, Length=255, Percent_Identity=30.9803921568627, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI19921524, Length=225, Percent_Identity=30.6666666666667, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36577; Mature: 36577

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDHHLPLPELSEPFSIGSVTIRNRAVLAPMSGVTDLPFRQLAWRYGAGLVVTEMVASRE
CCCCCCCCCCCCCCCCCCEEEECCCEEECCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
LVANRGESWARLKNAGMVPHMVQLAGREAHFMAEAAKIAADNGAGIIDINMGCPAKKVTG
HHHHCCCHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHCC
GYSGSALMRDPDHALSLIEATVGAVDVPVTLKMRLGWDENSINAPEIARRAEAAGVQLIT
CCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCEEEEE
IHGRTRMQFYEGRADWDAIRAVREVISVPLIANGDVETAEDAREILRRSGADAVMVGRGA
EECCEEEEEECCCCCHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHCCCCEEEECCCC
QGQPWLPAVLAGHSAPQRADIPTIAVEHYEMMLEFYGREAGLRHARKHLGWYLDRSAPDI
CCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCEEECCCCCCC
ATPEKAQIMTSRDTGEVADLLRSALSENAGEEAARKAA
CCCCHHEEEECCCCHHHHHHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure
MKDHHLPLPELSEPFSIGSVTIRNRAVLAPMSGVTDLPFRQLAWRYGAGLVVTEMVASRE
CCCCCCCCCCCCCCCCCCEEEECCCEEECCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
LVANRGESWARLKNAGMVPHMVQLAGREAHFMAEAAKIAADNGAGIIDINMGCPAKKVTG
HHHHCCCHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHCC
GYSGSALMRDPDHALSLIEATVGAVDVPVTLKMRLGWDENSINAPEIARRAEAAGVQLIT
CCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCEEEEE
IHGRTRMQFYEGRADWDAIRAVREVISVPLIANGDVETAEDAREILRRSGADAVMVGRGA
EECCEEEEEECCCCCHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHCCCCEEEECCCC
QGQPWLPAVLAGHSAPQRADIPTIAVEHYEMMLEFYGREAGLRHARKHLGWYLDRSAPDI
CCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCEEECCCCCCC
ATPEKAQIMTSRDTGEVADLLRSALSENAGEEAARKAA
CCCCHHEEEECCCCHHHHHHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8412703 [H]