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The map label for this gene is eno

Identifier: 15888752

GI number: 15888752

Start: 1421590

End: 1422864

Strand: Direct

Name: eno

Synonym: Atu1426

Alternate gene names: 15888752

Gene position: 1421590-1422864 (Clockwise)

Preceding gene: 159184755

Following gene: 17935327

Centisome position: 50.03

GC content: 59.69

Gene sequence:

>1275_bases
ATGACTGCCATTACCGATATCATCGCGCGCGAAATTCTCGACAGCCGCGGCAATCCGACCGTTGAAGTCGATGTTTACCT
CGAAGACGGCTCCATGGGCCGTGCGGCCGTTCCGTCCGGCGCCTCCACTGGCGCGCATGAGGCGGTTGAGCTGCGCGATG
GCGGCAAGCGTTATCTCGGCAAGGGCGTGGAAAAGGCGGTCGAAGCCGTCAATACCGAAATCTTCGACGCCATCGGCGGT
TTCGACGCTGAAAACCAGATCCAGATCGATCAGATGATGATCGCGCTTGACGGCACGCCGAACAAGTCGCGCCTCGGCGC
CAACGCCATCCTCGGCGTCTCGCTTGCCATCGCCAAGGCTGCGGCTGAAGCCTCCGGCCTGCCGCTTTACCGTTATGTTG
GCGGTCCGAACGCCCATCTGCTTCCGGTTCCGATGATGAACATCATCAACGGCGGCGCGCATGCCGATAACCCCATCGAC
TTCCAGGAATTCATGATCCTGCCGGTTGGCGCGGAAAACATCCGCGAAGCCGTGCGTATGGGTTCGGAAGTGTTCCACAC
GCTGAAAAAAGAACTGTCCGCACAGGGCCACAACACCAATGTCGGTGATGAGGGCGGTTTTGCACCGGGCCTCGAGAGCG
CGCCGGCAGCCCTCGATTTCATCATGAAGTCGATCGAAAAGGCTGGCTACCGTCCGGGCGAAGACATGTATGTCGGCCTC
GACTGCGCATCGACCGAGTTCTTTAAGGACGGTAAGTACGTTCTGGAAGGTGAAGGCCGCACGCTGGAGCCCGGCGCCAT
GGCCGAATATCTGGCCGAGCTTGTCAATAAATACCCGATCATCTCGGTTGAAGACGGCATGGCCGAAGATGACTGGGAAG
GCTGGAAGACGCTGACTGATCTCGTCGGCAACAAATGCCAGCTCGTCGGCGACGATCTCTTCGTCACCAACTCCGCGCGT
CTTCGCGACGGCATCAAGATGGGCGTCGCCAACTCCATTCTCGTCAAGGTTAACCAGATCGGTTCGCTTTCCGAGACGCT
GGATGCAGTCGAAACGGCACACAAGGCCGGTTACACCGCCGTCATGTCGCACCGCTCTGGCGAGACGGAAGATTCTACCA
TCGCCGATCTCGCTGTCGCCACCAACTGCGGTCAGATCAAGACCGGTTCGCTTGCCCGTTCTGACCGGCTCGCAAAGTAC
AACCAGCTGATCCGCATTGAGGAAATGCTGGGTCCGCAGGCTGCCTACGCCGGCCGTTCGATCCTGCGCGGGTAA

Upstream 100 bases:

>100_bases
GCGGGCGCTCCGTTTCTGCCGCCCGCCGTCATTGTAATTTCTTCGTCATCGTCTAAGACAGGCGACGGAAAATATCTGTT
CCATTCCCAAGGGAGAGACC

Downstream 100 bases:

>100_bases
TTCGCCAGGATTTTGTGGGAGGTCGTTACTGCCCGTGACGGGATTGGTATCAGGCCCAGCAACACATTCGACGTCATCCT
CGGCCTTGTGCCGAGGATCT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MTAITDIIAREILDSRGNPTVEVDVYLEDGSMGRAAVPSGASTGAHEAVELRDGGKRYLGKGVEKAVEAVNTEIFDAIGG
FDAENQIQIDQMMIALDGTPNKSRLGANAILGVSLAIAKAAAEASGLPLYRYVGGPNAHLLPVPMMNIINGGAHADNPID
FQEFMILPVGAENIREAVRMGSEVFHTLKKELSAQGHNTNVGDEGGFAPGLESAPAALDFIMKSIEKAGYRPGEDMYVGL
DCASTEFFKDGKYVLEGEGRTLEPGAMAEYLAELVNKYPIISVEDGMAEDDWEGWKTLTDLVGNKCQLVGDDLFVTNSAR
LRDGIKMGVANSILVKVNQIGSLSETLDAVETAHKAGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRLAKY
NQLIRIEEMLGPQAAYAGRSILRG

Sequences:

>Translated_424_residues
MTAITDIIAREILDSRGNPTVEVDVYLEDGSMGRAAVPSGASTGAHEAVELRDGGKRYLGKGVEKAVEAVNTEIFDAIGG
FDAENQIQIDQMMIALDGTPNKSRLGANAILGVSLAIAKAAAEASGLPLYRYVGGPNAHLLPVPMMNIINGGAHADNPID
FQEFMILPVGAENIREAVRMGSEVFHTLKKELSAQGHNTNVGDEGGFAPGLESAPAALDFIMKSIEKAGYRPGEDMYVGL
DCASTEFFKDGKYVLEGEGRTLEPGAMAEYLAELVNKYPIISVEDGMAEDDWEGWKTLTDLVGNKCQLVGDDLFVTNSAR
LRDGIKMGVANSILVKVNQIGSLSETLDAVETAHKAGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRLAKY
NQLIRIEEMLGPQAAYAGRSILRG
>Mature_423_residues
TAITDIIAREILDSRGNPTVEVDVYLEDGSMGRAAVPSGASTGAHEAVELRDGGKRYLGKGVEKAVEAVNTEIFDAIGGF
DAENQIQIDQMMIALDGTPNKSRLGANAILGVSLAIAKAAAEASGLPLYRYVGGPNAHLLPVPMMNIINGGAHADNPIDF
QEFMILPVGAENIREAVRMGSEVFHTLKKELSAQGHNTNVGDEGGFAPGLESAPAALDFIMKSIEKAGYRPGEDMYVGLD
CASTEFFKDGKYVLEGEGRTLEPGAMAEYLAELVNKYPIISVEDGMAEDDWEGWKTLTDLVGNKCQLVGDDLFVTNSARL
RDGIKMGVANSILVKVNQIGSLSETLDAVETAHKAGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRLAKYN
QLIRIEEMLGPQAAYAGRSILRG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=432, Percent_Identity=51.1574074074074, Blast_Score=411, Evalue=1e-115,
Organism=Homo sapiens, GI4503571, Length=432, Percent_Identity=50.462962962963, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI301897477, Length=433, Percent_Identity=50.1154734411085, Blast_Score=403, Evalue=1e-112,
Organism=Homo sapiens, GI301897469, Length=433, Percent_Identity=50.1154734411085, Blast_Score=403, Evalue=1e-112,
Organism=Homo sapiens, GI301897479, Length=431, Percent_Identity=45.9396751740139, Blast_Score=355, Evalue=4e-98,
Organism=Homo sapiens, GI169201331, Length=337, Percent_Identity=24.9258160237389, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI169201757, Length=337, Percent_Identity=24.9258160237389, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI239744207, Length=337, Percent_Identity=24.9258160237389, Blast_Score=100, Evalue=5e-21,
Organism=Escherichia coli, GI1789141, Length=430, Percent_Identity=61.6279069767442, Blast_Score=514, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI17536383, Length=432, Percent_Identity=51.8518518518518, Blast_Score=411, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI71995829, Length=432, Percent_Identity=51.8518518518518, Blast_Score=411, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI32563855, Length=190, Percent_Identity=45.7894736842105, Blast_Score=175, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6323985, Length=438, Percent_Identity=50.9132420091324, Blast_Score=404, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6324974, Length=438, Percent_Identity=50.4566210045662, Blast_Score=401, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6324969, Length=438, Percent_Identity=50.4566210045662, Blast_Score=401, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6321693, Length=439, Percent_Identity=48.5193621867882, Blast_Score=387, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6321968, Length=439, Percent_Identity=48.2915717539863, Blast_Score=370, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580918, Length=438, Percent_Identity=49.3150684931507, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580916, Length=438, Percent_Identity=49.3150684931507, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580920, Length=438, Percent_Identity=49.3150684931507, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580914, Length=438, Percent_Identity=49.3150684931507, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI281360527, Length=438, Percent_Identity=49.3150684931507, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI17137654, Length=438, Percent_Identity=49.3150684931507, Blast_Score=372, Evalue=1e-103,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_AGRT5 (Q8UFH1)

Other databases:

- EMBL:   AE007869
- PIR:   A97533
- PIR:   AB2752
- RefSeq:   NP_354433.1
- ProteinModelPortal:   Q8UFH1
- SMR:   Q8UFH1
- STRING:   Q8UFH1
- GeneID:   1133464
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu1426
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- PhylomeDB:   Q8UFH1
- ProtClustDB:   PRK00077
- BioCyc:   ATUM176299-1:ATU1426-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45143; Mature: 45011

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 204-204 ACT_SITE 336-336 BINDING 154-154 BINDING 163-163 BINDING 284-284 BINDING 311-311 BINDING 336-336 BINDING 387-387

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAITDIIAREILDSRGNPTVEVDVYLEDGSMGRAAVPSGASTGAHEAVELRDGGKRYLG
CCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCHHHHH
KGVEKAVEAVNTEIFDAIGGFDAENQIQIDQMMIALDGTPNKSRLGANAILGVSLAIAKA
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCHHHCCCHHHHHHHHHHHHH
AAEASGLPLYRYVGGPNAHLLPVPMMNIINGGAHADNPIDFQEFMILPVGAENIREAVRM
HHHHCCCEEEEECCCCCCEEECCCHHHHHCCCCCCCCCCCHHHEEEEECCHHHHHHHHHH
GSEVFHTLKKELSAQGHNTNVGDEGGFAPGLESAPAALDFIMKSIEKAGYRPGEDMYVGL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
DCASTEFFKDGKYVLEGEGRTLEPGAMAEYLAELVNKYPIISVEDGMAEDDWEGWKTLTD
ECCCCHHHCCCCEEEECCCCEECCHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHH
LVGNKCQLVGDDLFVTNSARLRDGIKMGVANSILVKVNQIGSLSETLDAVETAHKAGYTA
HHCCCEEEECCCEEEECCHHHHHHHHHCCCCEEEEEEHHCCCHHHHHHHHHHHHHCCCHH
VMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRLAKYNQLIRIEEMLGPQAAYAGRS
EEECCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ILRG
HHCC
>Mature Secondary Structure 
TAITDIIAREILDSRGNPTVEVDVYLEDGSMGRAAVPSGASTGAHEAVELRDGGKRYLG
CHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCHHHHH
KGVEKAVEAVNTEIFDAIGGFDAENQIQIDQMMIALDGTPNKSRLGANAILGVSLAIAKA
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCHHHCCCHHHHHHHHHHHHH
AAEASGLPLYRYVGGPNAHLLPVPMMNIINGGAHADNPIDFQEFMILPVGAENIREAVRM
HHHHCCCEEEEECCCCCCEEECCCHHHHHCCCCCCCCCCCHHHEEEEECCHHHHHHHHHH
GSEVFHTLKKELSAQGHNTNVGDEGGFAPGLESAPAALDFIMKSIEKAGYRPGEDMYVGL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
DCASTEFFKDGKYVLEGEGRTLEPGAMAEYLAELVNKYPIISVEDGMAEDDWEGWKTLTD
ECCCCHHHCCCCEEEECCCCEECCHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHH
LVGNKCQLVGDDLFVTNSARLRDGIKMGVANSILVKVNQIGSLSETLDAVETAHKAGYTA
HHCCCEEEECCCEEEECCHHHHHHHHHCCCCEEEEEEHHCCCHHHHHHHHHHHHHCCCHH
VMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRLAKYNQLIRIEEMLGPQAAYAGRS
EEECCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ILRG
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194