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The map label for this gene is birA [H]

Identifier: 15888613

GI number: 15888613

Start: 1271985

End: 1272755

Strand: Direct

Name: birA [H]

Synonym: Atu1284

Alternate gene names: 15888613

Gene position: 1271985-1272755 (Clockwise)

Preceding gene: 159184691

Following gene: 159184692

Centisome position: 44.76

GC content: 61.87

Gene sequence:

>771_bases
TTGAGCAGCACACACAAGCATACGGGCCGGATGTCCATTGATGACTTCCGGCACGAGGCGATGGCGGAGACGCCATCGAC
CAATCTGGAATGTTTCGCCCGGGCACGGGCGGGTGATGGCGGCAACCTCTGGGTTACCGCCATCCGCCAGACCGGGGGGC
GCGGCCGTCGCGGCCGGCCGTGGGTATCTGAACCCGGCAATCTCTATGCCTCGCTTCTTCTGATCGATCCCGCGCCTGTT
GAGCGTATCGGCTCCCTGCCGCTTGCTTTTGCGCTTGCCGTCTATCGCGCCATTCGCGCCGTGTTGCCGACAGCTGGTGA
GCCGCTCGAAATCAAATGGCCGAACGACGTGCTGATCGGTCGCCGCAAGACCTGCGGTATCCTGATGGAGGCAGAGCTTC
TGCCCGACGGGCGACGCGCCATCGTCATCGGCATCGGCATCAATATCGCCCATAAGCCGGACAATCCGCTTTATCCCGTC
ACCATGCTCTCCGAGCACGGTGCGTCCTGTTCGCCCGATGAGTTGTTCGCCCATCTCTTTCGCGAGACGGCGGAGGTTCT
GCGGAACTGGGATGAGGGCAGGGGCGTTTCCGGCATCATGACTGGCTGGAGGGCGGCGGCCTGCGGTATTGGCGAACATA
TCACCGTCAATTTCCCGGACCGTTCGATCGGTGGGCGTTTTGTCGGCATTGATGATAATGGTTATCTGCTGCTGGATGAG
GACGAGGGCGCAAGGCGTTCCATTGCCGCCGGCGACGTGTTTTTCGGATGA

Upstream 100 bases:

>100_bases
TGAAAGTCGTTTATGCGCTTTCGGCCCTGTTCGTTGTCGCCTTCGTCGTCTTTGGCGGTGCACTTGGCAACGCGGTGACT
GTTGCGGCGAAGACCTTCTT

Downstream 100 bases:

>100_bases
GGTGAACGGTGCGGCCCAGGAACCCGCGCCGAACCGGATCCCACAGGTTGGAGCTTCCTGCAGCTCATGAAATGCAGGGT
GACAAACCGGTTCCGCATCG

Product: birA bifunctional protein

Products: NA

Alternate protein names: Biotin--protein ligase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MSSTHKHTGRMSIDDFRHEAMAETPSTNLECFARARAGDGGNLWVTAIRQTGGRGRRGRPWVSEPGNLYASLLLIDPAPV
ERIGSLPLAFALAVYRAIRAVLPTAGEPLEIKWPNDVLIGRRKTCGILMEAELLPDGRRAIVIGIGINIAHKPDNPLYPV
TMLSEHGASCSPDELFAHLFRETAEVLRNWDEGRGVSGIMTGWRAAACGIGEHITVNFPDRSIGGRFVGIDDNGYLLLDE
DEGARRSIAAGDVFFG

Sequences:

>Translated_256_residues
MSSTHKHTGRMSIDDFRHEAMAETPSTNLECFARARAGDGGNLWVTAIRQTGGRGRRGRPWVSEPGNLYASLLLIDPAPV
ERIGSLPLAFALAVYRAIRAVLPTAGEPLEIKWPNDVLIGRRKTCGILMEAELLPDGRRAIVIGIGINIAHKPDNPLYPV
TMLSEHGASCSPDELFAHLFRETAEVLRNWDEGRGVSGIMTGWRAAACGIGEHITVNFPDRSIGGRFVGIDDNGYLLLDE
DEGARRSIAAGDVFFG
>Mature_255_residues
SSTHKHTGRMSIDDFRHEAMAETPSTNLECFARARAGDGGNLWVTAIRQTGGRGRRGRPWVSEPGNLYASLLLIDPAPVE
RIGSLPLAFALAVYRAIRAVLPTAGEPLEIKWPNDVLIGRRKTCGILMEAELLPDGRRAIVIGIGINIAHKPDNPLYPVT
MLSEHGASCSPDELFAHLFRETAEVLRNWDEGRGVSGIMTGWRAAACGIGEHITVNFPDRSIGGRFVGIDDNGYLLLDED
EGARRSIAAGDVFFG

Specific function: Activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins [H]

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=224, Percent_Identity=31.25, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR003142
- InterPro:   IPR004143 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 27827; Mature: 27696

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTHKHTGRMSIDDFRHEAMAETPSTNLECFARARAGDGGNLWVTAIRQTGGRGRRGRP
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCCCCC
WVSEPGNLYASLLLIDPAPVERIGSLPLAFALAVYRAIRAVLPTAGEPLEIKWPNDVLIG
CCCCCCCEEEEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEC
RRKTCGILMEAELLPDGRRAIVIGIGINIAHKPDNPLYPVTMLSEHGASCSPDELFAHLF
CCCCCCEEEEEEECCCCCEEEEEEEEEEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHH
RETAEVLRNWDEGRGVSGIMTGWRAAACGIGEHITVNFPDRSIGGRFVGIDDNGYLLLDE
HHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCCCEEEEEC
DEGARRSIAAGDVFFG
CCCCCCEEECCCCCCC
>Mature Secondary Structure 
SSTHKHTGRMSIDDFRHEAMAETPSTNLECFARARAGDGGNLWVTAIRQTGGRGRRGRP
CCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCCCCC
WVSEPGNLYASLLLIDPAPVERIGSLPLAFALAVYRAIRAVLPTAGEPLEIKWPNDVLIG
CCCCCCCEEEEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEC
RRKTCGILMEAELLPDGRRAIVIGIGINIAHKPDNPLYPVTMLSEHGASCSPDELFAHLF
CCCCCCEEEEEEECCCCCEEEEEEEEEEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHH
RETAEVLRNWDEGRGVSGIMTGWRAAACGIGEHITVNFPDRSIGGRFVGIDDNGYLLLDE
HHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCCCEEEEEC
DEGARRSIAAGDVFFG
CCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]