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The map label for this gene is nuoB [H]

Identifier: 15888598

GI number: 15888598

Start: 1256555

End: 1257160

Strand: Direct

Name: nuoB [H]

Synonym: Atu1269

Alternate gene names: 15888598

Gene position: 1256555-1257160 (Clockwise)

Preceding gene: 15888597

Following gene: 15888599

Centisome position: 44.22

GC content: 62.05

Gene sequence:

>606_bases
GTGGAAGAAGGGAGCGCTGGAATGGGCATGAGCCAGAACAACAGCACGCTCGTTGCGCCGCAGCCGAAGGGGATCATCGA
TCCCGCAACCGGCAAGCCGGTTGGCAGCAACGACGCCTACTTCACGGAAATCAATGACGAGCTGGCCGACAAGGGCTTTC
TCGTCACCTCGACCGACGAGCTGATCACCTGGGCGCGCACCGGCTCGCTGATGTGGATGCAGTTCGGTCTCGCCTGCTGC
GCCGTTGAAGGCCTGATGCAGGCTTCCGGACCGCGTTATGATATGGAGCGCTTCGGTGTCGCCCCGCGCGCCTCACCGCG
CCAGTCGGACGTCATGATCGTTGCCGGCACGCTGACCAACAAGATGGCGCCCGCTCTGCGCAAGGTCTATGACCAGATGC
CTGAGCCGCGTTACGTCATCTCCATGGGCTCCTGCGCCAATGGCGGCGGTTATTACCATTATTCCTACGCCGTGGTGCGT
GGCTGTGACCGCGTTGTGCCGGTGGATATCTACGTTCCGGGCTGTCCCCCCACGGCGGAGGCGCTGCTTTACGGCGTGCT
TCTGCTGCAAAAGAAAATTCGTCGTACCGGTACGATCGAGCGCTAA

Upstream 100 bases:

>100_bases
TCCTGTTTCCCTGGGCGGTGTCTTTCGGCGAGATGGGTTGGTTCGGTTTCTGGTCGATGATGGTGTTCCTCGCGGTCCTC
ACCATCGGCTTTATCTATGA

Downstream 100 bases:

>100_bases
GGGCAAAGGACGGATTTCGGAATGAGCGAAGCTCTCAATGATCTTGCTGCTTACGTCAAGGAAGCGCGTGGTTCTCTCGT
GGTATCGGCCGATATCGCCT

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MEEGSAGMGMSQNNSTLVAPQPKGIIDPATGKPVGSNDAYFTEINDELADKGFLVTSTDELITWARTGSLMWMQFGLACC
AVEGLMQASGPRYDMERFGVAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYAVVR
GCDRVVPVDIYVPGCPPTAEALLYGVLLLQKKIRRTGTIER

Sequences:

>Translated_201_residues
MEEGSAGMGMSQNNSTLVAPQPKGIIDPATGKPVGSNDAYFTEINDELADKGFLVTSTDELITWARTGSLMWMQFGLACC
AVEGLMQASGPRYDMERFGVAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYAVVR
GCDRVVPVDIYVPGCPPTAEALLYGVLLLQKKIRRTGTIER
>Mature_201_residues
MEEGSAGMGMSQNNSTLVAPQPKGIIDPATGKPVGSNDAYFTEINDELADKGFLVTSTDELITWARTGSLMWMQFGLACC
AVEGLMQASGPRYDMERFGVAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYAVVR
GCDRVVPVDIYVPGCPPTAEALLYGVLLLQKKIRRTGTIER

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=148, Percent_Identity=76.3513513513514, Blast_Score=244, Evalue=4e-65,
Organism=Escherichia coli, GI1788624, Length=169, Percent_Identity=43.7869822485207, Blast_Score=149, Evalue=1e-37,
Organism=Escherichia coli, GI1789074, Length=123, Percent_Identity=43.0894308943089, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1788834, Length=124, Percent_Identity=40.3225806451613, Blast_Score=98, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17509685, Length=143, Percent_Identity=69.2307692307692, Blast_Score=219, Evalue=9e-58,
Organism=Drosophila melanogaster, GI18859983, Length=142, Percent_Identity=76.056338028169, Blast_Score=239, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24642371, Length=142, Percent_Identity=76.056338028169, Blast_Score=239, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24651058, Length=142, Percent_Identity=75.3521126760563, Blast_Score=234, Evalue=3e-62,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 21786; Mature: 21786

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEGSAGMGMSQNNSTLVAPQPKGIIDPATGKPVGSNDAYFTEINDELADKGFLVTSTDE
CCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEECCHHHHCCCEEEECHHH
LITWARTGSLMWMQFGLACCAVEGLMQASGPRYDMERFGVAPRASPRQSDVMIVAGTLTN
HEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCEEEEEECHHH
KMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYAVVRGCDRVVPVDIYVPGCPPTAE
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEHHHHCCCCEEEEEEEECCCCCHHH
ALLYGVLLLQKKIRRTGTIER
HHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MEEGSAGMGMSQNNSTLVAPQPKGIIDPATGKPVGSNDAYFTEINDELADKGFLVTSTDE
CCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEECCHHHHCCCEEEECHHH
LITWARTGSLMWMQFGLACCAVEGLMQASGPRYDMERFGVAPRASPRQSDVMIVAGTLTN
HEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCEEEEEECHHH
KMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYAVVRGCDRVVPVDIYVPGCPPTAE
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEHHHHCCCCEEEEEEEECCCCCHHH
ALLYGVLLLQKKIRRTGTIER
HHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA