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The map label for this gene is purF [H]

Identifier: 15888418

GI number: 15888418

Start: 1066974

End: 1068482

Strand: Reverse

Name: purF [H]

Synonym: Atu1075

Alternate gene names: 15888418

Gene position: 1068482-1066974 (Counterclockwise)

Preceding gene: 15888419

Following gene: 15888417

Centisome position: 37.6

GC content: 59.91

Gene sequence:

>1509_bases
ATGATTGAGCCGCTTTCGCATTCCCCGTTTTCTGGGTCCACTTTCAAGGAAGAGATCGATGGCGATACGCTGCATGAAGA
ATGCGGCGTTTTCGGCATTCTCGGCCATGACGATGCCTCGGCGCTGACCGCTCTCGGCCTGCATGCGCTTCAGCATCGCG
GCCAGGAAGCAGCCGGCATCGTCTCCTTTGATGGCAAGCGCTTCCATCAGGAGCGCCATATGGGCCTCGTCGGCGATCAT
TATACCGATCCGGCAACCCTTGCCCGCCTGCCCGGCTCCATCGCGATTGGCCACACCCGTTATTCCACGACAGGCGAAGT
GGCGATGCGCAATGTGCAGCCGCTGTTTGCCGAGCTGGAAGAAGGCGGCATTTCGATTGCCCATAACGGCAATTTCACCA
ACGGCCTGACGCTGCGCCGCCAGATCATCGCCACCGGCGCCATCTGTCAGTCGACATCGGATACCGAAGTCGTGCTGCAT
CTCATCGCCCGTTCACGCCACTCTTCCACCGCCGATCGTTTCATCGATGCTATCAGGCAGATGGAAGGCGGTTATTCGAT
GCTGGCGATGACGCGGACCAAGCTGATCGCCGCCCGCGACCCGATCGGCATCCGCCCGCTGGTCATGGGAGAGCTTGATG
GCAAGCCGATCTTCTGCTCGGAAACCTGTGCTCTCGACATCATCGGCGCGAAATTCGTGCGCGACGTGGAAAACGGCGAA
GTCATCATCTGCGAAATCCAGCCTGACGGTTCTATCACCATCGATGCCCGCAAGCCCTCGAAGCCGCAGCCGGAGCGTCT
CTGCCTGTTCGAATATGTCTATTTCGCCCGTCCCGATTCCGTCGTCGGCGGCCGCAACGTCTATACGACGCGCAAGAACA
TGGGCATGAACCTTGCCAAGGAAGCGCCGCTGGAAGCCGATGTCGTGGTTCCGGTTCCCGATGGCGGCACGCCGGCAGCG
CTCGGTTTTGCGCAGGCAAGCGGTATTCCCTTCGAATACGGCATCATCCGCAACCATTATGTCGGTCGCACCTTCATTGA
GCCGACACAGCAGATCCGCGCTTTCGGCGTCAAGCTCAAGCATTCCGCCAACCGGGCGATGATCGAAGGCAAGCGCGTGG
TGCTGGTGGATGATTCCATCGTGCGCGGCACGACTTCGGTGAAGATCGTGCAGATGATCCGCGAGGCGGGCGCCAAGGAA
GTGCATATCCGCGTCGCCAGCCCGATGATCTTCCATCCGGATTTCTACGGCATCGACACGCCTGACGCTGATAAGCTGCT
CGCCAACCAATATGCCGATGTGGACGCCATGGCGAAATTCATTGGTGCCGATTCGCTGGCTTTCCTGTCGATTGACGGGC
TGTACCGCGCCGTTGGCGGTGAAAACCGCAACCACGCGCGCCCGCAGTTCACCGACCATTATTTCACCGGCGATTATCCG
ACCCGTCTTCTCGACCAGAACGGCGAGGCCATGGGCAGCAAGATTTCCATGCTGGCCAGCAACGGCTGA

Upstream 100 bases:

>100_bases
TTTGTGCTAAATATCAAGCACGACACAGATGGGCCGACCGGAAGAGATACTTACCGGAGGGGTCTTTCTTTTTTCTCGGT
AGAGAGCAAGGGCAAGCCGG

Downstream 100 bases:

>100_bases
TTTTTAACAACGACAAAGCGGCCATCGGCTTCCGGGGCCGCTTTTTTCTTGCCTTTTGTTCAGGCCGGTGCGTGCGACGA
TATGATCGCTTGCGAAAGGC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MIEPLSHSPFSGSTFKEEIDGDTLHEECGVFGILGHDDASALTALGLHALQHRGQEAAGIVSFDGKRFHQERHMGLVGDH
YTDPATLARLPGSIAIGHTRYSTTGEVAMRNVQPLFAELEEGGISIAHNGNFTNGLTLRRQIIATGAICQSTSDTEVVLH
LIARSRHSSTADRFIDAIRQMEGGYSMLAMTRTKLIAARDPIGIRPLVMGELDGKPIFCSETCALDIIGAKFVRDVENGE
VIICEIQPDGSITIDARKPSKPQPERLCLFEYVYFARPDSVVGGRNVYTTRKNMGMNLAKEAPLEADVVVPVPDGGTPAA
LGFAQASGIPFEYGIIRNHYVGRTFIEPTQQIRAFGVKLKHSANRAMIEGKRVVLVDDSIVRGTTSVKIVQMIREAGAKE
VHIRVASPMIFHPDFYGIDTPDADKLLANQYADVDAMAKFIGADSLAFLSIDGLYRAVGGENRNHARPQFTDHYFTGDYP
TRLLDQNGEAMGSKISMLASNG

Sequences:

>Translated_502_residues
MIEPLSHSPFSGSTFKEEIDGDTLHEECGVFGILGHDDASALTALGLHALQHRGQEAAGIVSFDGKRFHQERHMGLVGDH
YTDPATLARLPGSIAIGHTRYSTTGEVAMRNVQPLFAELEEGGISIAHNGNFTNGLTLRRQIIATGAICQSTSDTEVVLH
LIARSRHSSTADRFIDAIRQMEGGYSMLAMTRTKLIAARDPIGIRPLVMGELDGKPIFCSETCALDIIGAKFVRDVENGE
VIICEIQPDGSITIDARKPSKPQPERLCLFEYVYFARPDSVVGGRNVYTTRKNMGMNLAKEAPLEADVVVPVPDGGTPAA
LGFAQASGIPFEYGIIRNHYVGRTFIEPTQQIRAFGVKLKHSANRAMIEGKRVVLVDDSIVRGTTSVKIVQMIREAGAKE
VHIRVASPMIFHPDFYGIDTPDADKLLANQYADVDAMAKFIGADSLAFLSIDGLYRAVGGENRNHARPQFTDHYFTGDYP
TRLLDQNGEAMGSKISMLASNG
>Mature_502_residues
MIEPLSHSPFSGSTFKEEIDGDTLHEECGVFGILGHDDASALTALGLHALQHRGQEAAGIVSFDGKRFHQERHMGLVGDH
YTDPATLARLPGSIAIGHTRYSTTGEVAMRNVQPLFAELEEGGISIAHNGNFTNGLTLRRQIIATGAICQSTSDTEVVLH
LIARSRHSSTADRFIDAIRQMEGGYSMLAMTRTKLIAARDPIGIRPLVMGELDGKPIFCSETCALDIIGAKFVRDVENGE
VIICEIQPDGSITIDARKPSKPQPERLCLFEYVYFARPDSVVGGRNVYTTRKNMGMNLAKEAPLEADVVVPVPDGGTPAA
LGFAQASGIPFEYGIIRNHYVGRTFIEPTQQIRAFGVKLKHSANRAMIEGKRVVLVDDSIVRGTTSVKIVQMIREAGAKE
VHIRVASPMIFHPDFYGIDTPDADKLLANQYADVDAMAKFIGADSLAFLSIDGLYRAVGGENRNHARPQFTDHYFTGDYP
TRLLDQNGEAMGSKISMLASNG

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=471, Percent_Identity=39.9150743099788, Blast_Score=337, Evalue=2e-92,
Organism=Escherichia coli, GI1788651, Length=481, Percent_Identity=40.7484407484407, Blast_Score=304, Evalue=8e-84,
Organism=Escherichia coli, GI1790167, Length=220, Percent_Identity=29.5454545454545, Blast_Score=77, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=484, Percent_Identity=39.0495867768595, Blast_Score=299, Evalue=2e-81,
Organism=Saccharomyces cerevisiae, GI6323958, Length=506, Percent_Identity=34.1897233201581, Blast_Score=240, Evalue=3e-64,
Organism=Drosophila melanogaster, GI24659598, Length=506, Percent_Identity=39.9209486166008, Blast_Score=337, Evalue=2e-92,
Organism=Drosophila melanogaster, GI28573187, Length=510, Percent_Identity=39.6078431372549, Blast_Score=335, Evalue=4e-92,
Organism=Drosophila melanogaster, GI24659604, Length=478, Percent_Identity=40.7949790794979, Blast_Score=330, Evalue=1e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 54624; Mature: 54624

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEPLSHSPFSGSTFKEEIDGDTLHEECGVFGILGHDDASALTALGLHALQHRGQEAAGI
CCCCCCCCCCCCCCHHHHCCCCHHHHHCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCEE
VSFDGKRFHQERHMGLVGDHYTDPATLARLPGSIAIGHTRYSTTGEVAMRNVQPLFAELE
EEECCHHHHHHHCCCCCCCCCCCCHHHHHCCCCEEECCEECCCCHHHHHHHHHHHHHHHH
EGGISIAHNGNFTNGLTLRRQIIATGAICQSTSDTEVVLHLIARSRHSSTADRFIDAIRQ
CCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
MEGGYSMLAMTRTKLIAARDPIGIRPLVMGELDGKPIFCSETCALDIIGAKFVRDVENGE
HCCCCEEEEHHHHHHEEECCCCCCCEEEEECCCCCEEEECCCCEEEEHHHHHHHHCCCCC
VIICEIQPDGSITIDARKPSKPQPERLCLFEYVYFARPDSVVGGRNVYTTRKNMGMNLAK
EEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEHHCCCCCCHH
EAPLEADVVVPVPDGGTPAALGFAQASGIPFEYGIIRNHYVGRTFIEPTQQIRAFGVKLK
CCCCCCEEEEECCCCCCCHHEEHHHHCCCCEEECCHHCCCCCCHHCCHHHHHHHHCEEEE
HSANRAMIEGKRVVLVDDSIVRGTTSVKIVQMIREAGAKEVHIRVASPMIFHPDFYGIDT
CCCCCEEECCCEEEEECCCHHCCCHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCCCC
PDADKLLANQYADVDAMAKFIGADSLAFLSIDGLYRAVGGENRNHARPQFTDHYFTGDYP
CCHHHHHHHHCCCHHHHHHHHCCCCEEEEEECHHHHHHCCCCCCCCCCCCCCCEECCCCC
TRLLDQNGEAMGSKISMLASNG
HHHHCCCCCCCCCEEEEEECCC
>Mature Secondary Structure
MIEPLSHSPFSGSTFKEEIDGDTLHEECGVFGILGHDDASALTALGLHALQHRGQEAAGI
CCCCCCCCCCCCCCHHHHCCCCHHHHHCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCEE
VSFDGKRFHQERHMGLVGDHYTDPATLARLPGSIAIGHTRYSTTGEVAMRNVQPLFAELE
EEECCHHHHHHHCCCCCCCCCCCCHHHHHCCCCEEECCEECCCCHHHHHHHHHHHHHHHH
EGGISIAHNGNFTNGLTLRRQIIATGAICQSTSDTEVVLHLIARSRHSSTADRFIDAIRQ
CCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
MEGGYSMLAMTRTKLIAARDPIGIRPLVMGELDGKPIFCSETCALDIIGAKFVRDVENGE
HCCCCEEEEHHHHHHEEECCCCCCCEEEEECCCCCEEEECCCCEEEEHHHHHHHHCCCCC
VIICEIQPDGSITIDARKPSKPQPERLCLFEYVYFARPDSVVGGRNVYTTRKNMGMNLAK
EEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEHHCCCCCCHH
EAPLEADVVVPVPDGGTPAALGFAQASGIPFEYGIIRNHYVGRTFIEPTQQIRAFGVKLK
CCCCCCEEEEECCCCCCCHHEEHHHHCCCCEEECCHHCCCCCCHHCCHHHHHHHHCEEEE
HSANRAMIEGKRVVLVDDSIVRGTTSVKIVQMIREAGAKEVHIRVASPMIFHPDFYGIDT
CCCCCEEECCCEEEEECCCHHCCCHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCCCC
PDADKLLANQYADVDAMAKFIGADSLAFLSIDGLYRAVGGENRNHARPQFTDHYFTGDYP
CCHHHHHHHHCCCHHHHHHHHCCCCEEEEEECHHHHHHCCCCCCCCCCCCCCCEECCCCC
TRLLDQNGEAMGSKISMLASNG
HHHHCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA