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The map label for this gene is gsh1

Identifier: 15887998

GI number: 15887998

Start: 649842

End: 651215

Strand: Reverse

Name: gsh1

Synonym: Atu0656

Alternate gene names: NA

Gene position: 651215-649842 (Counterclockwise)

Preceding gene: 229007176

Following gene: 15887996

Centisome position: 22.92

GC content: 61.14

Gene sequence:

>1374_bases
ATGGCACGCGACACGACCGACCAGACACCCCTCTCCTCCGTCACGGAGCTTACCGACTATCTGGCGGCCGGTTGCAGGGC
TGAAGCGGATTTCCGCATCGGCACCGAACATGAGAAATTCGCCTTTTTCCGCAAGGATAACAGCCCTGTTCCCTATTTCG
GCGAGGCCAGCATTTCGGCACTGCTGAAGGGCATGCAGGAAAAGCTCGGCTGGGAGCCGATCATGGACGGTGAAAACATC
ATCGGCCTTGGCGAACAGCACGGCATGGGCGCCATCTCCATCGAGCCGGGCGGCCAGTTCGAACTGTCTGGCGCGCCGCT
CGAAAACCTGCACCAGACCTGCAAGGAATCCAACCAGCATCTGGCAACCCTGCGGGAAGTGGCCGAGCCGATGGGCATCC
GTTTCCTCGGCATCGGCGGCAGCCCGCTGTGGACTTCCGACGAGACCCCGCGCATGCCGAAATCGCGCTACGCGATCATG
ACGCGCTACATGCCGAAAGTCGGGACCAAGGGTCTCGACATGATGTACCGCACCTGCACCATCCAGGTTAACCTCGACTT
CTCCTCCGAAGCGGACATGCGCCAGAAGATGCGCGTTTCCATGAAGTTGCAATCGCTGGCGACGGCGCTCTTTGCCTCTT
CGCCCTTTACCGAGGGCAAGCCTAACGGTCTTCTGTCCTGGCGCGGCGATATCTGGCGCGATACCGACAATCGCCGCTCC
GGCGTGCTGCCCTTCACCTTCAGTGACGATTTCGGCTTCAAGGATTATGTCGAATGGGCGCTTGATGTGCCGATGTATTT
CATCGTGCGCGACGGCAAATATCACGATTGCACCCATGTCACCTTCCGCCAGTTCATGAACGGGGCGCTGAAGGGTGAAA
TCGCCGCATGGGAACCGACCATGGGCGACTGGACGAACCACCTCTCCACGCTGTTCCCCGATGTGCGCCTGAAGCGTTTC
CTCGAAATGCGCGGCGCCGATGGCGGCCCGTGGCGGCGCATCTGCGCCCTGCCCGCCTTCTGGGTCGGCCTTCTCTATAA
TCAGGAGGCCCTCGACGCTGCCGATGCGCTGACCGCCGATTGGAGCTTCGATGAGGTGATCGCACTGCGCAACGCCGTGC
CAGCGAAGGGGCTGGCGGCCGAGATTGCCGGCAAGCCGCTTCTCGGCATTGCCCGCCAGGTGCTCGACATTTCCCGTACC
GGCCTGAAAAACCGCAAGCGGCTGAATGGCGAAGGTCAGGACGAGACCGTGTTCCTGTCATCGCTAGACGAGGTTCTGGC
CAAGAAGACCACGCTCGCCGAAGACCTGCTTGCACTTTATAACGGCCGCTGGGGCGGTTCGGTGGTGCCGGTGTTCGAGG
AATATCAGTATTGA

Upstream 100 bases:

>100_bases
ATGCATCTTTAAAAGAAATTCATTTGCCACCGGATACGATCCGGCCCTATCTGCCAATCATATGCCTCGCGGCAACATTG
ACGTCATAACAAGGTAAGCC

Downstream 100 bases:

>100_bases
GGCATTGCCGGTAAGCTGCGCCGGTGAGGTAAAGCCGGGCCATAAAAAAGCCCGGCGCGGCCGAGGGGCCTGACACCGGG
CAAAGGCAGGGCTATTGGCA

Product: glutamate-cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Gamma-Glutamylcysteine Synthetase; Glutamate/Cysteine Ligase Family Protein; Glutamate-Cysteine Ligase GshA; Glutamylcysteine Synthetase; Glutamate-Cysteine Ligase Family 2 Protein; Glutamate-Cysteine Ligase Protein; Glutathione Synthetase; Glutamate-Cysteine Ligase Gsha; Glutamate-Cysteine Ligase Protein; Glutamate-Cysteine Ligase Family Protein; Glutamate Cystein Ligase Protein; Glutamate-Cysteine Ligase GSHA

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MARDTTDQTPLSSVTELTDYLAAGCRAEADFRIGTEHEKFAFFRKDNSPVPYFGEASISALLKGMQEKLGWEPIMDGENI
IGLGEQHGMGAISIEPGGQFELSGAPLENLHQTCKESNQHLATLREVAEPMGIRFLGIGGSPLWTSDETPRMPKSRYAIM
TRYMPKVGTKGLDMMYRTCTIQVNLDFSSEADMRQKMRVSMKLQSLATALFASSPFTEGKPNGLLSWRGDIWRDTDNRRS
GVLPFTFSDDFGFKDYVEWALDVPMYFIVRDGKYHDCTHVTFRQFMNGALKGEIAAWEPTMGDWTNHLSTLFPDVRLKRF
LEMRGADGGPWRRICALPAFWVGLLYNQEALDAADALTADWSFDEVIALRNAVPAKGLAAEIAGKPLLGIARQVLDISRT
GLKNRKRLNGEGQDETVFLSSLDEVLAKKTTLAEDLLALYNGRWGGSVVPVFEEYQY

Sequences:

>Translated_457_residues
MARDTTDQTPLSSVTELTDYLAAGCRAEADFRIGTEHEKFAFFRKDNSPVPYFGEASISALLKGMQEKLGWEPIMDGENI
IGLGEQHGMGAISIEPGGQFELSGAPLENLHQTCKESNQHLATLREVAEPMGIRFLGIGGSPLWTSDETPRMPKSRYAIM
TRYMPKVGTKGLDMMYRTCTIQVNLDFSSEADMRQKMRVSMKLQSLATALFASSPFTEGKPNGLLSWRGDIWRDTDNRRS
GVLPFTFSDDFGFKDYVEWALDVPMYFIVRDGKYHDCTHVTFRQFMNGALKGEIAAWEPTMGDWTNHLSTLFPDVRLKRF
LEMRGADGGPWRRICALPAFWVGLLYNQEALDAADALTADWSFDEVIALRNAVPAKGLAAEIAGKPLLGIARQVLDISRT
GLKNRKRLNGEGQDETVFLSSLDEVLAKKTTLAEDLLALYNGRWGGSVVPVFEEYQY
>Mature_456_residues
ARDTTDQTPLSSVTELTDYLAAGCRAEADFRIGTEHEKFAFFRKDNSPVPYFGEASISALLKGMQEKLGWEPIMDGENII
GLGEQHGMGAISIEPGGQFELSGAPLENLHQTCKESNQHLATLREVAEPMGIRFLGIGGSPLWTSDETPRMPKSRYAIMT
RYMPKVGTKGLDMMYRTCTIQVNLDFSSEADMRQKMRVSMKLQSLATALFASSPFTEGKPNGLLSWRGDIWRDTDNRRSG
VLPFTFSDDFGFKDYVEWALDVPMYFIVRDGKYHDCTHVTFRQFMNGALKGEIAAWEPTMGDWTNHLSTLFPDVRLKRFL
EMRGADGGPWRRICALPAFWVGLLYNQEALDAADALTADWSFDEVIALRNAVPAKGLAAEIAGKPLLGIARQVLDISRTG
LKNRKRLNGEGQDETVFLSSLDEVLAKKTTLAEDLLALYNGRWGGSVVPVFEEYQY

Specific function: Unknown

COG id: COG3572

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 51077; Mature: 50945

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDTTDQTPLSSVTELTDYLAAGCRAEADFRIGTEHEKFAFFRKDNSPVPYFGEASISA
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCHHEEEEECCCCCCCCCCCHHHHH
LLKGMQEKLGWEPIMDGENIIGLGEQHGMGAISIEPGGQFELSGAPLENLHQTCKESNQH
HHHHHHHHCCCCCCCCCCCEEECCCCCCCEEEEECCCCCEEECCCCHHHHHHHHHHHHHH
LATLREVAEPMGIRFLGIGGSPLWTSDETPRMPKSRYAIMTRYMPKVGTKGLDMMYRTCT
HHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHEEEEEEE
IQVNLDFSSEADMRQKMRVSMKLQSLATALFASSPFTEGKPNGLLSWRGDIWRDTDNRRS
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCC
GVLPFTFSDDFGFKDYVEWALDVPMYFIVRDGKYHDCTHVTFRQFMNGALKGEIAAWEPT
CEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCHHCCCEEECCCC
MGDWTNHLSTLFPDVRLKRFLEMRGADGGPWRRICALPAFWVGLLYNQEALDAADALTAD
CCHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
WSFDEVIALRNAVPAKGLAAEIAGKPLLGIARQVLDISRTGLKNRKRLNGEGQDETVFLS
CCHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCEEHHH
SLDEVLAKKTTLAEDLLALYNGRWGGSVVPVFEEYQY
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCC
>Mature Secondary Structure 
ARDTTDQTPLSSVTELTDYLAAGCRAEADFRIGTEHEKFAFFRKDNSPVPYFGEASISA
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCHHEEEEECCCCCCCCCCCHHHHH
LLKGMQEKLGWEPIMDGENIIGLGEQHGMGAISIEPGGQFELSGAPLENLHQTCKESNQH
HHHHHHHHCCCCCCCCCCCEEECCCCCCCEEEEECCCCCEEECCCCHHHHHHHHHHHHHH
LATLREVAEPMGIRFLGIGGSPLWTSDETPRMPKSRYAIMTRYMPKVGTKGLDMMYRTCT
HHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHEEEEEEE
IQVNLDFSSEADMRQKMRVSMKLQSLATALFASSPFTEGKPNGLLSWRGDIWRDTDNRRS
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCC
GVLPFTFSDDFGFKDYVEWALDVPMYFIVRDGKYHDCTHVTFRQFMNGALKGEIAAWEPT
CEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCHHCCCEEECCCC
MGDWTNHLSTLFPDVRLKRFLEMRGADGGPWRRICALPAFWVGLLYNQEALDAADALTAD
CCHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
WSFDEVIALRNAVPAKGLAAEIAGKPLLGIARQVLDISRTGLKNRKRLNGEGQDETVFLS
CCHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCEEHHH
SLDEVLAKKTTLAEDLLALYNGRWGGSVVPVFEEYQY
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA