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The map label for this gene is ipk

Identifier: 15887973

GI number: 15887973

Start: 619823

End: 620722

Strand: Reverse

Name: ipk

Synonym: Atu0632

Alternate gene names: 15887973

Gene position: 620722-619823 (Counterclockwise)

Preceding gene: 159184397

Following gene: 15887972

Centisome position: 21.84

GC content: 61.0

Gene sequence:

>900_bases
ATGCGCCTGCATGAGGTTTCCGGGGCCACCGAAACAGTCGAGGCGGCCCCGGCCAAGATCAATCTGGCGCTGCATGTCAC
CGGTCAGCGGGCGGATGGCTACCATCTGCTTGAAACGCTGGTGACATTCACCGCAGCCGGCGACATGATCCGCATTCGCG
ACGCCGCCACCGACAGTTTTTCCATTTCCGGCCCCTTCGGCGATCTTCTAAGTGCCGGCGATAGCGGCGATAACCTCGTC
ACCCGCGCCCGAGACATCTTGCGCGATGCTCTTGCGTCGACAGGCCAGCCCGCCCGCCCGGTCGACATTCACTTGGAAAA
GAACCTGCCGGTTGCTTCGGGCATCGGTGGCGGCTCGGCGGATGCCGCTGCAACGCTGCGGGGGCTTCTGCGGCACTGGG
ATGCCGCGATTGCACCGGAAAAGCTTAAGTCTCTCGCACTGAAACTGGGCGCGGATGTACCGATGTGCCTTGCAAGCCGG
CCGCTGATTGCGCGCGGCATCGGTGAGGACATCGAAGCCCTCACCGACCTGCCGGAACTCTCCATGGTTCTGGCCAATCC
GCTGAAGGCTGTCTCAACACCGGAGATTTTCCGGCGATTGCAGAACAAGGTCAATCCGCACCTGCCCACACCTTCGACGA
TTGGCTGGGCGACAACTGGCTGGATGGATTTTCTCGCCCAAAGCCGCAACGATCTGCAGCCGCCGGCTCAGGCGCTGCTG
CCTGAAATCGGGGAGATAACCGGGTTGCTGTCTGAGGAAGGGGCCACCCTTGTCCGCATGTCCGGTTCCGGGGCAACCTG
TTTCGGGATTTTTCATTCCTTCGATGCCGCCAAAAACGCGGAAACATCACTTAGGAAAAAACGTCCCGGCTGGTATTTTC
ACGCAACGCGGACCATTTGA

Upstream 100 bases:

>100_bases
AAAGGACGCTGAAAACACCGCCAAGAAGGACGTGGCGCCGCAACCGCCGGCCCCTCCGGCACCGGCGACACCTACGCCGG
ACAAGAAATCCTGACGCGGC

Downstream 100 bases:

>100_bases
GGGCCGGATATTTCGAGGATAAGGCCATAGCAGGCGATAGACCCTTCATTCCCCTTGGCATTGCGGTTCTGACCGTTTCG
GACAGCCGCACGCTGGAGAA

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MRLHEVSGATETVEAAPAKINLALHVTGQRADGYHLLETLVTFTAAGDMIRIRDAATDSFSISGPFGDLLSAGDSGDNLV
TRARDILRDALASTGQPARPVDIHLEKNLPVASGIGGGSADAAATLRGLLRHWDAAIAPEKLKSLALKLGADVPMCLASR
PLIARGIGEDIEALTDLPELSMVLANPLKAVSTPEIFRRLQNKVNPHLPTPSTIGWATTGWMDFLAQSRNDLQPPAQALL
PEIGEITGLLSEEGATLVRMSGSGATCFGIFHSFDAAKNAETSLRKKRPGWYFHATRTI

Sequences:

>Translated_299_residues
MRLHEVSGATETVEAAPAKINLALHVTGQRADGYHLLETLVTFTAAGDMIRIRDAATDSFSISGPFGDLLSAGDSGDNLV
TRARDILRDALASTGQPARPVDIHLEKNLPVASGIGGGSADAAATLRGLLRHWDAAIAPEKLKSLALKLGADVPMCLASR
PLIARGIGEDIEALTDLPELSMVLANPLKAVSTPEIFRRLQNKVNPHLPTPSTIGWATTGWMDFLAQSRNDLQPPAQALL
PEIGEITGLLSEEGATLVRMSGSGATCFGIFHSFDAAKNAETSLRKKRPGWYFHATRTI
>Mature_299_residues
MRLHEVSGATETVEAAPAKINLALHVTGQRADGYHLLETLVTFTAAGDMIRIRDAATDSFSISGPFGDLLSAGDSGDNLV
TRARDILRDALASTGQPARPVDIHLEKNLPVASGIGGGSADAAATLRGLLRHWDAAIAPEKLKSLALKLGADVPMCLASR
PLIARGIGEDIEALTDLPELSMVLANPLKAVSTPEIFRRLQNKVNPHLPTPSTIGWATTGWMDFLAQSRNDLQPPAQALL
PEIGEITGLLSEEGATLVRMSGSGATCFGIFHSFDAAKNAETSLRKKRPGWYFHATRTI

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=278, Percent_Identity=32.0143884892086, Blast_Score=107, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_AGRT5 (Q8UHP8)

Other databases:

- EMBL:   AE007869
- PIR:   AB2654
- PIR:   F97435
- RefSeq:   NP_353654.1
- ProteinModelPortal:   Q8UHP8
- SMR:   Q8UHP8
- STRING:   Q8UHP8
- GeneID:   1132670
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0632
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   RIYNGLR
- PhylomeDB:   Q8UHP8
- ProtClustDB:   PRK14608
- BioCyc:   ATUM176299-1:ATU0632-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31785; Mature: 31785

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: ACT_SITE 19-19 ACT_SITE 152-152

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLHEVSGATETVEAAPAKINLALHVTGQRADGYHLLETLVTFTAAGDMIRIRDAATDSF
CCCCCCCCCCHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCE
SISGPFGDLLSAGDSGDNLVTRARDILRDALASTGQPARPVDIHLEKNLPVASGIGGGSA
ECCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCH
DAAATLRGLLRHWDAAIAPEKLKSLALKLGADVPMCLASRPLIARGIGEDIEALTDLPEL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHCCCHHHHCCCHHHHHHHCCHHH
SMVLANPLKAVSTPEIFRRLQNKVNPHLPTPSTIGWATTGWMDFLAQSRNDLQPPAQALL
HHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHH
PEIGEITGLLSEEGATLVRMSGSGATCFGIFHSFDAAKNAETSLRKKRPGWYFHATRTI
HHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHHCCHHHHHHHCCCCEEEEEECCC
>Mature Secondary Structure
MRLHEVSGATETVEAAPAKINLALHVTGQRADGYHLLETLVTFTAAGDMIRIRDAATDSF
CCCCCCCCCCHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCE
SISGPFGDLLSAGDSGDNLVTRARDILRDALASTGQPARPVDIHLEKNLPVASGIGGGSA
ECCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCH
DAAATLRGLLRHWDAAIAPEKLKSLALKLGADVPMCLASRPLIARGIGEDIEALTDLPEL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHCCCHHHHCCCHHHHHHHCCHHH
SMVLANPLKAVSTPEIFRRLQNKVNPHLPTPSTIGWATTGWMDFLAQSRNDLQPPAQALL
HHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHH
PEIGEITGLLSEEGATLVRMSGSGATCFGIFHSFDAAKNAETSLRKKRPGWYFHATRTI
HHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHHCCHHHHHHHCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194