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The map label for this gene is cusS [H]

Identifier: 15887969

GI number: 15887969

Start: 616678

End: 618033

Strand: Reverse

Name: cusS [H]

Synonym: Atu0628

Alternate gene names: 15887969

Gene position: 618033-616678 (Counterclockwise)

Preceding gene: 15887970

Following gene: 159184391

Centisome position: 21.75

GC content: 58.04

Gene sequence:

>1356_bases
ATGCCCGGCGCTAGCTTTCTGAAGAGCACTGCCGTCAGACTGGCGATCATCTACATCGTCCTGTTTGTAACCGCATCGCT
TGGCGCGAACATCACGGCCTATCAGATGGTCGTGCGCTTCCTTGATGAACGCCTGAACGCCAATGTCCAGGAGCGTTACC
GCGAGATCGCATCGGCCTTCGAAGCACGCGGCCTTCAGGGCGCCGTGGACATGATCGACAATCACGGCCTCGCCATCAGG
GGGCAGGAGACCATCTACGCCTTGCGCAACCCCGCCGGACAACTGCTTGCGGGCAGCAATCGATTTTCGAACGTCCCATC
CGGCTATTCGATCCTTGAACCTGACGACCATCACGGCAGTGCGTCCCACTACAAGCTTTTCAGCGGTCCTTTGGGGCCGA
ACACTCTCGTGGTGGGGATCAATTCCGGGGACACGGATGAACTGGCCCGTATCGTTCTCATCAGTTTCGGATGTGCGACC
GCGATCGTTCTTTCCGTAGGCATGGCGGGAGCCGCCATATTGGCGTATCGAACGCGCCACCGTATCTCGTCGCTGGCGCA
AATCGCCCACGCTATCGGACATGGCGAGCTTTCGCGACGACTTCCGGTGTCATCGCGCATGGATGAAATCGACATATTGT
CAGCGGAAATCAATGTGGCACTGGCAAGACTGGACTCCAGCGTCGCGGCATTGAAGCAGGTGACGATCGACGTCGCCCAT
GACCTCAAGACCCCAATCGGCCGCACATTCCTGGTACTGGAAGACGCGCTCAATGCGCACACGGTTGACGAAATGAGGAT
CGGAGTCGAAACCGCGCTTGAAGAACTGCAATCCATTGCAAATACGTTCGATGCTCTTCTACGGATCGCCCAGATCGAAG
CGCGGAGCAGGACCGCCCGCTTCATCGCCTTCGATCTATGCGACGTCGTCGGCGACATCTACGAAACCTATGAGGTTATT
GCGGAAGAGGAGGGCTATGAGCTGTCGATGCGCGGTGCCGCGAGCGGCTGCCGGATCAATGGTGATCCTGATCTCATTCG
CCAATTGCTGGCGAACCTGTTGTCCAACGCCATGCGGCACACCCCCGCCGGGTCAAGCATTTCGCTCGGCGTATCGCGCC
AGGATGGACGGATCTTGCTAGATATCGCCGATAACGGACCAGGCATATCAGATGAAGACCGGGCGCAGGTGTTCGACCGG
TTCTATCGCGTTGAGAAAAGTAGAACCACGGCGGGATCCGGGCTCGGCCTGAGCCTTGTCAAAGCCATAGCGGAATTACA
CGGTGCCATCGTAACGCTTGCGGACAACAATCCCGGTTTGCGCGTGGTCGTTCAGTTTCAGGAATTGCGCGTCTGA

Upstream 100 bases:

>100_bases
TCCGTCGTGGAAACGCACATCAGCCGGCTGAGGGCGAAAATCGACAAACCGTTTGATCAGCCACTTCTGCATACCGTCAA
AAACACGGGCTATACGCTTC

Downstream 100 bases:

>100_bases
GGCAGGCTTTGGTTTGCTTCACCCCGAAAGCTGGCGCAAACCGTAATGCCATGTTTGGCCCATGGTGAACCTTTCCATTT
CCGGAAAAGCTGTCCCATGG

Product: two component sensor kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MPGASFLKSTAVRLAIIYIVLFVTASLGANITAYQMVVRFLDERLNANVQERYREIASAFEARGLQGAVDMIDNHGLAIR
GQETIYALRNPAGQLLAGSNRFSNVPSGYSILEPDDHHGSASHYKLFSGPLGPNTLVVGINSGDTDELARIVLISFGCAT
AIVLSVGMAGAAILAYRTRHRISSLAQIAHAIGHGELSRRLPVSSRMDEIDILSAEINVALARLDSSVAALKQVTIDVAH
DLKTPIGRTFLVLEDALNAHTVDEMRIGVETALEELQSIANTFDALLRIAQIEARSRTARFIAFDLCDVVGDIYETYEVI
AEEEGYELSMRGAASGCRINGDPDLIRQLLANLLSNAMRHTPAGSSISLGVSRQDGRILLDIADNGPGISDEDRAQVFDR
FYRVEKSRTTAGSGLGLSLVKAIAELHGAIVTLADNNPGLRVVVQFQELRV

Sequences:

>Translated_451_residues
MPGASFLKSTAVRLAIIYIVLFVTASLGANITAYQMVVRFLDERLNANVQERYREIASAFEARGLQGAVDMIDNHGLAIR
GQETIYALRNPAGQLLAGSNRFSNVPSGYSILEPDDHHGSASHYKLFSGPLGPNTLVVGINSGDTDELARIVLISFGCAT
AIVLSVGMAGAAILAYRTRHRISSLAQIAHAIGHGELSRRLPVSSRMDEIDILSAEINVALARLDSSVAALKQVTIDVAH
DLKTPIGRTFLVLEDALNAHTVDEMRIGVETALEELQSIANTFDALLRIAQIEARSRTARFIAFDLCDVVGDIYETYEVI
AEEEGYELSMRGAASGCRINGDPDLIRQLLANLLSNAMRHTPAGSSISLGVSRQDGRILLDIADNGPGISDEDRAQVFDR
FYRVEKSRTTAGSGLGLSLVKAIAELHGAIVTLADNNPGLRVVVQFQELRV
>Mature_450_residues
PGASFLKSTAVRLAIIYIVLFVTASLGANITAYQMVVRFLDERLNANVQERYREIASAFEARGLQGAVDMIDNHGLAIRG
QETIYALRNPAGQLLAGSNRFSNVPSGYSILEPDDHHGSASHYKLFSGPLGPNTLVVGINSGDTDELARIVLISFGCATA
IVLSVGMAGAAILAYRTRHRISSLAQIAHAIGHGELSRRLPVSSRMDEIDILSAEINVALARLDSSVAALKQVTIDVAHD
LKTPIGRTFLVLEDALNAHTVDEMRIGVETALEELQSIANTFDALLRIAQIEARSRTARFIAFDLCDVVGDIYETYEVIA
EEEGYELSMRGAASGCRINGDPDLIRQLLANLLSNAMRHTPAGSSISLGVSRQDGRILLDIADNGPGISDEDRAQVFDRF
YRVEKSRTTAGSGLGLSLVKAIAELHGAIVTLADNNPGLRVVVQFQELRV

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=356, Percent_Identity=28.3707865168539, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1788279, Length=280, Percent_Identity=29.2857142857143, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788393, Length=279, Percent_Identity=28.3154121863799, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1790551, Length=220, Percent_Identity=29.0909090909091, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1790346, Length=268, Percent_Identity=25.3731343283582, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1786600, Length=203, Percent_Identity=30.5418719211823, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789808, Length=328, Percent_Identity=28.6585365853659, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1789403, Length=456, Percent_Identity=24.5614035087719, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790861, Length=291, Percent_Identity=24.3986254295533, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788713, Length=244, Percent_Identity=26.2295081967213, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1786912, Length=213, Percent_Identity=28.169014084507, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1787894, Length=317, Percent_Identity=24.9211356466877, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789149, Length=245, Percent_Identity=25.3061224489796, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081816, Length=93, Percent_Identity=40.8602150537634, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 48646; Mature: 48515

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGASFLKSTAVRLAIIYIVLFVTASLGANITAYQMVVRFLDERLNANVQERYREIASAF
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
EARGLQGAVDMIDNHGLAIRGQETIYALRNPAGQLLAGSNRFSNVPSGYSILEPDDHHGS
HHCCCCHHHHHHCCCCEEEECCHHEEEHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCC
ASHYKLFSGPLGPNTLVVGINSGDTDELARIVLISFGCATAIVLSVGMAGAAILAYRTRH
CCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RISSLAQIAHAIGHGELSRRLPVSSRMDEIDILSAEINVALARLDSSVAALKQVTIDVAH
HHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLKTPIGRTFLVLEDALNAHTVDEMRIGVETALEELQSIANTFDALLRIAQIEARSRTAR
HHCCCCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIAFDLCDVVGDIYETYEVIAEEEGYELSMRGAASGCRINGDPDLIRQLLANLLSNAMRH
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEECCCHHHHHHHHHHHHHHHHHC
TPAGSSISLGVSRQDGRILLDIADNGPGISDEDRAQVFDRFYRVEKSRTTAGSGLGLSLV
CCCCCCEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
KAIAELHGAIVTLADNNPGLRVVVQFQELRV
HHHHHHCCEEEEEECCCCCEEEEEEEHHHCC
>Mature Secondary Structure 
PGASFLKSTAVRLAIIYIVLFVTASLGANITAYQMVVRFLDERLNANVQERYREIASAF
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
EARGLQGAVDMIDNHGLAIRGQETIYALRNPAGQLLAGSNRFSNVPSGYSILEPDDHHGS
HHCCCCHHHHHHCCCCEEEECCHHEEEHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCC
ASHYKLFSGPLGPNTLVVGINSGDTDELARIVLISFGCATAIVLSVGMAGAAILAYRTRH
CCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RISSLAQIAHAIGHGELSRRLPVSSRMDEIDILSAEINVALARLDSSVAALKQVTIDVAH
HHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLKTPIGRTFLVLEDALNAHTVDEMRIGVETALEELQSIANTFDALLRIAQIEARSRTAR
HHCCCCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIAFDLCDVVGDIYETYEVIAEEEGYELSMRGAASGCRINGDPDLIRQLLANLLSNAMRH
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEECCCHHHHHHHHHHHHHHHHHC
TPAGSSISLGVSRQDGRILLDIADNGPGISDEDRAQVFDRFYRVEKSRTTAGSGLGLSLV
CCCCCCEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
KAIAELHGAIVTLADNNPGLRVVVQFQELRV
HHHHHHCCEEEEEECCCCCEEEEEEEHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]