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The map label for this gene is fadA [H]

Identifier: 15887849

GI number: 15887849

Start: 491832

End: 493040

Strand: Direct

Name: fadA [H]

Synonym: Atu0502

Alternate gene names: 15887849

Gene position: 491832-493040 (Clockwise)

Preceding gene: 159184345

Following gene: 15887850

Centisome position: 17.31

GC content: 62.7

Gene sequence:

>1209_bases
ATGACCGACGTTTACATTTACGACCATGTCCGCACTCCGCGCGGACGCGGCAAGAAGGATGGCAGCCTGCATGAGGTGCC
TTCCGTTCGCCTGGCCGCCAAAACGCTGGAAGCCATCCGCGATCGTAACGGGCTCGACACCTCTGCTGTCGATGACATCA
TCATGGGCTGCGTCGATCCCGTCATGGATGCCGGTGCGGTGATCCCGAAGGCTGCCGCCTTCGAGGCCGGTTACTCCAAC
AGGGCCCCGGGCATGCAGATCTCGCGCTTCTGCGCCTCGGGTCTCGACGCCATCAATTTCGCGGCCGGCAAGGTCAAGGC
GGGCTCGGACGATATCGTCATTGCCGGTGGCGTGGAAAGCATGTCGCGCGTCGGCATGGGCATGTCGGGCGGCGCTTGGT
ACATGGATCCCTCGGTGAACTTCCCCGCCTTCTTCATGCCGCAGGGCGTTTCGGCTGATCTCATCGCCACCAAATATGGT
TTCTCGCGTGACGACGTCGACGCCTATGCTGTCGAAAGCCAGAAGCGTGCGGCGCATGCCTGGGAAAACGGCTATTTCAA
CAAGTCCGTGATTCCGGTGAAGGACCAGAACGGGCTGACGATCCTCGACCGCGACGAGCATATGCGCCCCGGCACGGACA
TGCAGGCGTTGGCTTCGCTCAACCCTTCCTTCCAGATGCCGGGCGAAATGGGCGGTTTCGAAGCCGTTGGCATCATGGCG
CATCCGGAAGTGGAGCGTATCAACTACGTGCACCATGCGGGCAACTCGTCCGGCATCGTCGATGGCGCTGCCGCCGTGCT
GGTCGGCTCCAAGGCGGGCGGCGAGGCGATGGGCGTGAAACCGCGTGCCCGCATCAAGGCTTTCGCCAATATCGGCTCCG
ACCCGGCGCTGATGCTGACCGGCCCGGTGGATGTGACCGAGAAGCTTCTGAAAAAGACCGGCATGTCGATTGCCGATATC
GATCTTTTCGAACTCAACGAAGCCTTCGCCGCCGTGGTCCTGCGTTACATGCAGGCTTTCGACATCAGCCACGACCGGAT
GAACGTCAATGGCGGCGCCATCGCCATGGGCCATCCGCTGGGTGCCACCGGGGCGATGATCCTCGGCACGGTACTGGATG
AGCTGGAGCGGCGCGATCTGAACACCGCGCTCGTCACGCTCTGCATCGGCGCGGGCATGGGCACGGCGACGGTTATCGAG
CGCGTTTGA

Upstream 100 bases:

>100_bases
GCAAGGCCCGCATCGAAACCGGCGCGGACACCATGATGGAACTGGCTGCGGAAGCGTTCTGAGACATCTGGCGCTTACGA
GCGCCACAGGGAGAGAGTTT

Downstream 100 bases:

>100_bases
ATTTGCGGAGGGCAACCCAACCTCCTCATCCCTGTGCTTGTCACAGGGATCCAGCAGCGTCGCGTCTGCGGCGCGAAAGA
GTCTTCTAGCCCAAAGACTT

Product: acetyl-CoA acetyltransferase

Products: CoA; 3-oxoacyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MTDVYIYDHVRTPRGRGKKDGSLHEVPSVRLAAKTLEAIRDRNGLDTSAVDDIIMGCVDPVMDAGAVIPKAAAFEAGYSN
RAPGMQISRFCASGLDAINFAAGKVKAGSDDIVIAGGVESMSRVGMGMSGGAWYMDPSVNFPAFFMPQGVSADLIATKYG
FSRDDVDAYAVESQKRAAHAWENGYFNKSVIPVKDQNGLTILDRDEHMRPGTDMQALASLNPSFQMPGEMGGFEAVGIMA
HPEVERINYVHHAGNSSGIVDGAAAVLVGSKAGGEAMGVKPRARIKAFANIGSDPALMLTGPVDVTEKLLKKTGMSIADI
DLFELNEAFAAVVLRYMQAFDISHDRMNVNGGAIAMGHPLGATGAMILGTVLDELERRDLNTALVTLCIGAGMGTATVIE
RV

Sequences:

>Translated_402_residues
MTDVYIYDHVRTPRGRGKKDGSLHEVPSVRLAAKTLEAIRDRNGLDTSAVDDIIMGCVDPVMDAGAVIPKAAAFEAGYSN
RAPGMQISRFCASGLDAINFAAGKVKAGSDDIVIAGGVESMSRVGMGMSGGAWYMDPSVNFPAFFMPQGVSADLIATKYG
FSRDDVDAYAVESQKRAAHAWENGYFNKSVIPVKDQNGLTILDRDEHMRPGTDMQALASLNPSFQMPGEMGGFEAVGIMA
HPEVERINYVHHAGNSSGIVDGAAAVLVGSKAGGEAMGVKPRARIKAFANIGSDPALMLTGPVDVTEKLLKKTGMSIADI
DLFELNEAFAAVVLRYMQAFDISHDRMNVNGGAIAMGHPLGATGAMILGTVLDELERRDLNTALVTLCIGAGMGTATVIE
RV
>Mature_401_residues
TDVYIYDHVRTPRGRGKKDGSLHEVPSVRLAAKTLEAIRDRNGLDTSAVDDIIMGCVDPVMDAGAVIPKAAAFEAGYSNR
APGMQISRFCASGLDAINFAAGKVKAGSDDIVIAGGVESMSRVGMGMSGGAWYMDPSVNFPAFFMPQGVSADLIATKYGF
SRDDVDAYAVESQKRAAHAWENGYFNKSVIPVKDQNGLTILDRDEHMRPGTDMQALASLNPSFQMPGEMGGFEAVGIMAH
PEVERINYVHHAGNSSGIVDGAAAVLVGSKAGGEAMGVKPRARIKAFANIGSDPALMLTGPVDVTEKLLKKTGMSIADID
LFELNEAFAAVVLRYMQAFDISHDRMNVNGGAIAMGHPLGATGAMILGTVLDELERRDLNTALVTLCIGAGMGTATVIER
V

Specific function: Thiolytic Cleavage Of Beta-Ketoadipyl-CoA To Succinate And Acetyl-CoA (By Similarity). [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4501853, Length=410, Percent_Identity=33.1707317073171, Blast_Score=204, Evalue=9e-53,
Organism=Homo sapiens, GI148539872, Length=407, Percent_Identity=32.4324324324324, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI167614485, Length=411, Percent_Identity=31.8734793187348, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI4557237, Length=418, Percent_Identity=30.3827751196172, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI4504327, Length=447, Percent_Identity=29.082774049217, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI194353979, Length=124, Percent_Identity=36.2903225806452, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1787663, Length=426, Percent_Identity=38.4976525821596, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI48994986, Length=409, Percent_Identity=37.4083129584352, Blast_Score=248, Evalue=4e-67,
Organism=Escherichia coli, GI87082165, Length=417, Percent_Identity=37.8896882494005, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1788554, Length=419, Percent_Identity=35.0835322195704, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1788683, Length=447, Percent_Identity=26.8456375838926, Blast_Score=122, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI133906874, Length=413, Percent_Identity=32.2033898305085, Blast_Score=201, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI17535921, Length=423, Percent_Identity=31.4420803782506, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI25147385, Length=416, Percent_Identity=28.8461538461538, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17551802, Length=449, Percent_Identity=26.9487750556793, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17535917, Length=407, Percent_Identity=25.0614250614251, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322031, Length=361, Percent_Identity=32.6869806094183, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6325229, Length=423, Percent_Identity=29.7872340425532, Blast_Score=149, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24655093, Length=415, Percent_Identity=34.4578313253012, Blast_Score=228, Evalue=8e-60,
Organism=Drosophila melanogaster, GI17648125, Length=414, Percent_Identity=33.5748792270531, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24640423, Length=418, Percent_Identity=30.1435406698565, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI17137578, Length=448, Percent_Identity=28.7946428571429, Blast_Score=159, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16

Molecular weight: Translated: 42516; Mature: 42385

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVYIYDHVRTPRGRGKKDGSLHEVPSVRLAAKTLEAIRDRNGLDTSAVDDIIMGCVDP
CCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VMDAGAVIPKAAAFEAGYSNRAPGMQISRFCASGLDAINFAAGKVKAGSDDIVIAGGVES
HHHCCCCCCCHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHCCCEEECCCCCEEEECCHHH
MSRVGMGMSGGAWYMDPSVNFPAFFMPQGVSADLIATKYGFSRDDVDAYAVESQKRAAHA
HHHHCCCCCCCEEEECCCCCCCEEECCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHH
WENGYFNKSVIPVKDQNGLTILDRDEHMRPGTDMQALASLNPSFQMPGEMGGFEAVGIMA
HHCCCCCCCEEEEECCCCCEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEEE
HPEVERINYVHHAGNSSGIVDGAAAVLVGSKAGGEAMGVKPRARIKAFANIGSDPALMLT
CCCHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCEEEEE
GPVDVTEKLLKKTGMSIADIDLFELNEAFAAVVLRYMQAFDISHDRMNVNGGAIAMGHPL
CCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEECCCC
GATGAMILGTVLDELERRDLNTALVTLCIGAGMGTATVIERV
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure 
TDVYIYDHVRTPRGRGKKDGSLHEVPSVRLAAKTLEAIRDRNGLDTSAVDDIIMGCVDP
CEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VMDAGAVIPKAAAFEAGYSNRAPGMQISRFCASGLDAINFAAGKVKAGSDDIVIAGGVES
HHHCCCCCCCHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHCCCEEECCCCCEEEECCHHH
MSRVGMGMSGGAWYMDPSVNFPAFFMPQGVSADLIATKYGFSRDDVDAYAVESQKRAAHA
HHHHCCCCCCCEEEECCCCCCCEEECCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHH
WENGYFNKSVIPVKDQNGLTILDRDEHMRPGTDMQALASLNPSFQMPGEMGGFEAVGIMA
HHCCCCCCCEEEEECCCCCEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEEE
HPEVERINYVHHAGNSSGIVDGAAAVLVGSKAGGEAMGVKPRARIKAFANIGSDPALMLT
CCCHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCEEEEE
GPVDVTEKLLKKTGMSIADIDLFELNEAFAAVVLRYMQAFDISHDRMNVNGGAIAMGHPL
CCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEECCCC
GATGAMILGTVLDELERRDLNTALVTLCIGAGMGTATVIERV
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acetyl-CoA

Specific reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]