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The map label for this gene is metZ [H]

Identifier: 15887779

GI number: 15887779

Start: 428128

End: 429312

Strand: Reverse

Name: metZ [H]

Synonym: Atu0432

Alternate gene names: 15887779

Gene position: 429312-428128 (Counterclockwise)

Preceding gene: 159184306

Following gene: 159184305

Centisome position: 15.11

GC content: 60.42

Gene sequence:

>1185_bases
ATGAGCAATAAATGGCGCCCGGCAACCCAGCTCGTTCACGGCGGTACGCTACGCTCACCCTATGGTGAAACGTCGGAAGC
GATCTATCTGACCCAGGGTTTCGTTTACGAAAACTCCGAAGCCGCCGAAGCCCGCTTCAAGGGCGAAACGGATGGCTTCA
TCTACGCCCGCTACGCCAGCCCGACCAATGACATGTTCGAAAAGCGCATGTGCCTGCTGGAAGGCGCGGAAGACGCGCGC
GCGCTTGCCTCCGGCATGGCCGCCGTCACCGCCGCCATCATGTGCCAGCTGCGCGCCGGCGATCACATCGTTGCCGCACG
AGCCCTTTTCGGCTCCTGCCGCTGGGTGGTGGAAACGCTCGCACCGAAATACGGCATCGAATGCACGCTGATCGACGGAC
GTGATCTCGACAATTGGGAAAAGGCGATCCAGCCGAACACCAAGCTGTTCTTCCTTGAAAGCCCGACCAACCCGACGCTC
GAAGTGGTTGATATTGCCGGTGTGGCGAAGCTCGCCAACCAGATCGGCGCGAAAGTGGTGGTCGATAACGTCTTTGCGAC
GCCGCTTTTCCAGAAGCCGCTCGAACTCGGCGCCCATATCGTGGTTTATTCGGCCACCAAACATATTGACGGCCAGGGCC
GCTGCCTGGGTGGCGTGATCCTCTCCGACAAGAAGTGGATCGAAGAAGAGCTGCAGGACTATTTCCGCCATACGGGCCCG
GCCATGTCGCCGTTCAACGCCTGGACGCTTCTGAAGGGCATCGAGACCCTGCCGCTTCGCGTCAAGCAGCAGACGGAGAA
TGCCGCGAAGATCGCCGATTTCCTCGCCGATAGCGGCAAGGTCGCCAAGGTCATCTATCCCGGCCGCGCCGATCATCCGC
AGGCGGATATCATCGCACGGCAGATGTCCGGAGGCTCCACTTTGGTGGCCTTCGAACTGAAGGGCGGCAAGGACGCAGCC
TTCAAGTTGCAGAACGCGCTTGAGATCGTGAAGATTTCCAACAATCTCGGCGATGCCAAGAGCCTCATCACCCATCCCGG
TACGACGACACACAAGAACCTCTCCGACGAGGCGCGTGCCGAACTCGGCATTTCCGGAGGCACCCTGCGCCTTTCCTGCG
GCATCGAGGATACCGACGATCTGCTGGAAGATCTCGCAAAGGGTCTTGCGGCCGTTTCCGCCTGA

Upstream 100 bases:

>100_bases
CCGGTCGGCTTGCAGCCACGTTAAACAAGTGGCTAAAAAGACCGGGTGCTAGGAACCGGTCTGCTTTTGCGGCCGGTTTT
TTTGTTTTGAAGGTGATCAC

Downstream 100 bases:

>100_bases
AAATTCAATCGGCAAATCGCCATGGCCCGACCCAGGTGTCGGGCCATGGTTTGCCTGATCGGCCGCAAAAGTCCGCAACA
AGCAACCCATATTTACCTTT

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MSNKWRPATQLVHGGTLRSPYGETSEAIYLTQGFVYENSEAAEARFKGETDGFIYARYASPTNDMFEKRMCLLEGAEDAR
ALASGMAAVTAAIMCQLRAGDHIVAARALFGSCRWVVETLAPKYGIECTLIDGRDLDNWEKAIQPNTKLFFLESPTNPTL
EVVDIAGVAKLANQIGAKVVVDNVFATPLFQKPLELGAHIVVYSATKHIDGQGRCLGGVILSDKKWIEEELQDYFRHTGP
AMSPFNAWTLLKGIETLPLRVKQQTENAAKIADFLADSGKVAKVIYPGRADHPQADIIARQMSGGSTLVAFELKGGKDAA
FKLQNALEIVKISNNLGDAKSLITHPGTTTHKNLSDEARAELGISGGTLRLSCGIEDTDDLLEDLAKGLAAVSA

Sequences:

>Translated_394_residues
MSNKWRPATQLVHGGTLRSPYGETSEAIYLTQGFVYENSEAAEARFKGETDGFIYARYASPTNDMFEKRMCLLEGAEDAR
ALASGMAAVTAAIMCQLRAGDHIVAARALFGSCRWVVETLAPKYGIECTLIDGRDLDNWEKAIQPNTKLFFLESPTNPTL
EVVDIAGVAKLANQIGAKVVVDNVFATPLFQKPLELGAHIVVYSATKHIDGQGRCLGGVILSDKKWIEEELQDYFRHTGP
AMSPFNAWTLLKGIETLPLRVKQQTENAAKIADFLADSGKVAKVIYPGRADHPQADIIARQMSGGSTLVAFELKGGKDAA
FKLQNALEIVKISNNLGDAKSLITHPGTTTHKNLSDEARAELGISGGTLRLSCGIEDTDDLLEDLAKGLAAVSA
>Mature_393_residues
SNKWRPATQLVHGGTLRSPYGETSEAIYLTQGFVYENSEAAEARFKGETDGFIYARYASPTNDMFEKRMCLLEGAEDARA
LASGMAAVTAAIMCQLRAGDHIVAARALFGSCRWVVETLAPKYGIECTLIDGRDLDNWEKAIQPNTKLFFLESPTNPTLE
VVDIAGVAKLANQIGAKVVVDNVFATPLFQKPLELGAHIVVYSATKHIDGQGRCLGGVILSDKKWIEEELQDYFRHTGPA
MSPFNAWTLLKGIETLPLRVKQQTENAAKIADFLADSGKVAKVIYPGRADHPQADIIARQMSGGSTLVAFELKGGKDAAF
KLQNALEIVKISNNLGDAKSLITHPGTTTHKNLSDEARAELGISGGTLRLSCGIEDTDDLLEDLAKGLAAVSA

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=368, Percent_Identity=34.2391304347826, Blast_Score=212, Evalue=6e-55,
Organism=Homo sapiens, GI299473759, Length=368, Percent_Identity=30.7065217391304, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI25453487, Length=367, Percent_Identity=28.6103542234332, Blast_Score=149, Evalue=5e-36,
Organism=Escherichia coli, GI1790375, Length=339, Percent_Identity=33.9233038348083, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1789383, Length=392, Percent_Identity=26.7857142857143, Blast_Score=145, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17560056, Length=332, Percent_Identity=38.855421686747, Blast_Score=241, Evalue=5e-64,
Organism=Caenorhabditis elegans, GI17538055, Length=332, Percent_Identity=36.7469879518072, Blast_Score=237, Evalue=9e-63,
Organism=Caenorhabditis elegans, GI71980708, Length=345, Percent_Identity=33.9130434782609, Blast_Score=193, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6319307, Length=389, Percent_Identity=36.2467866323907, Blast_Score=229, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6323333, Length=412, Percent_Identity=32.2815533980583, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6321254, Length=410, Percent_Identity=27.5609756097561, Blast_Score=140, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6321170, Length=288, Percent_Identity=31.9444444444444, Blast_Score=132, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6321904, Length=395, Percent_Identity=27.0886075949367, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI19922554, Length=348, Percent_Identity=35.3448275862069, Blast_Score=228, Evalue=6e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 42570; Mature: 42439

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKWRPATQLVHGGTLRSPYGETSEAIYLTQGFVYENSEAAEARFKGETDGFIYARYAS
CCCCCCCHHHHCCCCCCCCCCCCCCCEEEEEECEEEECCCHHHHHCCCCCCCEEEEEECC
PTNDMFEKRMCLLEGAEDARALASGMAAVTAAIMCQLRAGDHIVAARALFGSCRWVVETL
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHH
APKYGIECTLIDGRDLDNWEKAIQPNTKLFFLESPTNPTLEVVDIAGVAKLANQIGAKVV
CCCCCCEEEEECCCCCCHHHHHCCCCCEEEEEECCCCCCEEEEEHHHHHHHHHHCCCEEE
VDNVFATPLFQKPLELGAHIVVYSATKHIDGQGRCLGGVILSDKKWIEEELQDYFRHTGP
EHHHHHCHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCC
AMSPFNAWTLLKGIETLPLRVKQQTENAAKIADFLADSGKVAKVIYPGRADHPQADIIAR
CCCCCHHHHHHHCCHHCCEEEHHHCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHH
QMSGGSTLVAFELKGGKDAAFKLQNALEIVKISNNLGDAKSLITHPGTTTHKNLSDEARA
HCCCCCEEEEEEECCCCCHHHHHHHHEEEEEECCCCCCHHHHHCCCCCCCCCCCCHHHHH
ELGISGGTLRLSCGIEDTDDLLEDLAKGLAAVSA
HCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNKWRPATQLVHGGTLRSPYGETSEAIYLTQGFVYENSEAAEARFKGETDGFIYARYAS
CCCCCCHHHHCCCCCCCCCCCCCCCEEEEEECEEEECCCHHHHHCCCCCCCEEEEEECC
PTNDMFEKRMCLLEGAEDARALASGMAAVTAAIMCQLRAGDHIVAARALFGSCRWVVETL
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHH
APKYGIECTLIDGRDLDNWEKAIQPNTKLFFLESPTNPTLEVVDIAGVAKLANQIGAKVV
CCCCCCEEEEECCCCCCHHHHHCCCCCEEEEEECCCCCCEEEEEHHHHHHHHHHCCCEEE
VDNVFATPLFQKPLELGAHIVVYSATKHIDGQGRCLGGVILSDKKWIEEELQDYFRHTGP
EHHHHHCHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCC
AMSPFNAWTLLKGIETLPLRVKQQTENAAKIADFLADSGKVAKVIYPGRADHPQADIIAR
CCCCCHHHHHHHCCHHCCEEEHHHCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHH
QMSGGSTLVAFELKGGKDAAFKLQNALEIVKISNNLGDAKSLITHPGTTTHKNLSDEARA
HCCCCCEEEEEEECCCCCHHHHHHHHEEEEEECCCCCCHHHHHCCCCCCCCCCCCHHHHH
ELGISGGTLRLSCGIEDTDDLLEDLAKGLAAVSA
HCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]