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The map label for this gene is metK [H]

Identifier: 15887713

GI number: 15887713

Start: 356353

End: 357615

Strand: Direct

Name: metK [H]

Synonym: Atu0362

Alternate gene names: 15887713

Gene position: 356353-357615 (Clockwise)

Preceding gene: 159184276

Following gene: 15887714

Centisome position: 12.54

GC content: 61.2

Gene sequence:

>1263_bases
ATGCGTGCCAATTACCTGTTCACCAGCGAGTCCGTGGCCGAGGGTCATCCGGACAAGGTTTGTGATCGTATTTCCGATGA
AATCGTGGATCTTATTTACCGTGAGGCGGCCAAGACGGGCGTTGACCCATGGACCGTGCGCATCGCATGCGAAACGCTTG
CGACGACCAACCGCGTTGTTATCGCCGGTGAGGTTCGGGTTCCCGACACGCTTCTGAAGAAGGACAAGGACGGCAAGGTC
GTCAAGGACGCCGCCGGCCACCCGGTCATCAACCCTTCCAAGTTCAAGTCCGCCGCCCGAAAGGCGATCCGCGACATCGG
CTACGAACAGGATGGTTTCCACTGGAAGACCGCCAAGATCGACGTTCTGCTGCATCCGCAGTCGGCCGATATCGCGCAGG
GCGTGGATAATGCCTCCGACAAGCAGGGTGACGAGGGCGCTGGCGACCAGGGCATCATGTTCGGTTACGCTTGCAAGGAA
ACGCCGGACCTGATGCCGGCGCCGATCTATTATTCTCACCGCATCCTGCAGCTGCTGGCCACCGCCCGCAAGAGCGGCGA
AGGCGAAGCGGCGAAGCTCGGCCCCGATGCCAAGAGCCAGGTGACGGTTCGTTACGTCGACGGCAAGGCTTCGGAAGCCG
TATCCATCGTTCTGTCCACGCAGCACCTCGATGCCAGCTGGGACTCGAAGAAGGTCCGTGAGGTCGTCGAACCCTATATT
CGCGAGGCTCTGGGCGAGTTGAAGATCGCTGACGATTGCCAGTGGTATATCAACCCGACGGGCAAGTTCGTCATCGGCGG
TCCCGATGGCGATGCCGGCCTGACCGGCCGCAAGATCATCGTCGACACCTATGGCGGTGCTGCCCCCCATGGTGGTGGCG
CATTCTCCGGCAAGGACACGACCAAGGTCGACCGTTCGGCCGCTTATGCCGCCCGCTATCTCGCCAAGAACGTCGTGGCT
GCCGGCCTTGCCGAGCGCTGCACGATCCAGATCGCCTATGCGATCGGCGTTGCCCAGCCGCTGTCGATCTATGTCGATCT
GCACGGCACGGGCAAGGTCAGCGAAGATCAGGTCGAAGCCGCGATCCGCAAGGTCATGGACCTGTCGCCGTCGGGCATCC
GCCGTCATCTCGATCTCAACAAGCCGATCTACGCCAAGACGTCTTCTTACGGTCACTTCGGCCGCAAGGCTGGTCGTGAC
GGTTCCTTCTCCTGGGAGAAGCTCGATCTGGTGAAGCCGCTCAAGGAAGCTTTGAGCGCTTGA

Upstream 100 bases:

>100_bases
GGAATTCTCACATAAAGATATATTTATGTGGTTGTGTCCTTGTTTTTCGCGGCCGAAATGACTAACAAACTCCAAGACCA
TTCTTTGAGGGGAATCCCGC

Downstream 100 bases:

>100_bases
AGCATTTCCAGTAAAACTGCGTAGCGGTTTTACGTCCGGAGAATGCGGAGGCTTTTTCTAAGCCTTGTTATTTGGCGGTG
ATTGAGAGATACCGTCTACA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MRANYLFTSESVAEGHPDKVCDRISDEIVDLIYREAAKTGVDPWTVRIACETLATTNRVVIAGEVRVPDTLLKKDKDGKV
VKDAAGHPVINPSKFKSAARKAIRDIGYEQDGFHWKTAKIDVLLHPQSADIAQGVDNASDKQGDEGAGDQGIMFGYACKE
TPDLMPAPIYYSHRILQLLATARKSGEGEAAKLGPDAKSQVTVRYVDGKASEAVSIVLSTQHLDASWDSKKVREVVEPYI
REALGELKIADDCQWYINPTGKFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDTTKVDRSAAYAARYLAKNVVA
AGLAERCTIQIAYAIGVAQPLSIYVDLHGTGKVSEDQVEAAIRKVMDLSPSGIRRHLDLNKPIYAKTSSYGHFGRKAGRD
GSFSWEKLDLVKPLKEALSA

Sequences:

>Translated_420_residues
MRANYLFTSESVAEGHPDKVCDRISDEIVDLIYREAAKTGVDPWTVRIACETLATTNRVVIAGEVRVPDTLLKKDKDGKV
VKDAAGHPVINPSKFKSAARKAIRDIGYEQDGFHWKTAKIDVLLHPQSADIAQGVDNASDKQGDEGAGDQGIMFGYACKE
TPDLMPAPIYYSHRILQLLATARKSGEGEAAKLGPDAKSQVTVRYVDGKASEAVSIVLSTQHLDASWDSKKVREVVEPYI
REALGELKIADDCQWYINPTGKFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDTTKVDRSAAYAARYLAKNVVA
AGLAERCTIQIAYAIGVAQPLSIYVDLHGTGKVSEDQVEAAIRKVMDLSPSGIRRHLDLNKPIYAKTSSYGHFGRKAGRD
GSFSWEKLDLVKPLKEALSA
>Mature_420_residues
MRANYLFTSESVAEGHPDKVCDRISDEIVDLIYREAAKTGVDPWTVRIACETLATTNRVVIAGEVRVPDTLLKKDKDGKV
VKDAAGHPVINPSKFKSAARKAIRDIGYEQDGFHWKTAKIDVLLHPQSADIAQGVDNASDKQGDEGAGDQGIMFGYACKE
TPDLMPAPIYYSHRILQLLATARKSGEGEAAKLGPDAKSQVTVRYVDGKASEAVSIVLSTQHLDASWDSKKVREVVEPYI
REALGELKIADDCQWYINPTGKFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDTTKVDRSAAYAARYLAKNVVA
AGLAERCTIQIAYAIGVAQPLSIYVDLHGTGKVSEDQVEAAIRKVMDLSPSGIRRHLDLNKPIYAKTSSYGHFGRKAGRD
GSFSWEKLDLVKPLKEALSA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=410, Percent_Identity=44.6341463414634, Blast_Score=298, Evalue=4e-81,
Organism=Homo sapiens, GI4557737, Length=408, Percent_Identity=44.8529411764706, Blast_Score=294, Evalue=8e-80,
Organism=Escherichia coli, GI1789311, Length=418, Percent_Identity=48.3253588516746, Blast_Score=355, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI17538498, Length=410, Percent_Identity=45.1219512195122, Blast_Score=300, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI17538494, Length=410, Percent_Identity=44.8780487804878, Blast_Score=296, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI25145633, Length=413, Percent_Identity=44.0677966101695, Blast_Score=290, Evalue=8e-79,
Organism=Caenorhabditis elegans, GI17551082, Length=414, Percent_Identity=41.7874396135266, Blast_Score=281, Evalue=6e-76,
Organism=Caenorhabditis elegans, GI32565911, Length=320, Percent_Identity=47.8125, Blast_Score=255, Evalue=4e-68,
Organism=Caenorhabditis elegans, GI25145635, Length=320, Percent_Identity=46.875, Blast_Score=245, Evalue=3e-65,
Organism=Caenorhabditis elegans, GI32565905, Length=329, Percent_Identity=46.2006079027356, Blast_Score=243, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI32565903, Length=303, Percent_Identity=48.1848184818482, Blast_Score=241, Evalue=6e-64,
Organism=Saccharomyces cerevisiae, GI6320710, Length=414, Percent_Identity=43.2367149758454, Blast_Score=301, Evalue=2e-82,
Organism=Saccharomyces cerevisiae, GI6323209, Length=418, Percent_Identity=43.0622009569378, Blast_Score=300, Evalue=4e-82,
Organism=Drosophila melanogaster, GI24580527, Length=420, Percent_Identity=44.2857142857143, Blast_Score=291, Evalue=6e-79,
Organism=Drosophila melanogaster, GI24580525, Length=420, Percent_Identity=44.2857142857143, Blast_Score=291, Evalue=6e-79,
Organism=Drosophila melanogaster, GI45552159, Length=420, Percent_Identity=43.8095238095238, Blast_Score=285, Evalue=5e-77,
Organism=Drosophila melanogaster, GI24580529, Length=420, Percent_Identity=43.8095238095238, Blast_Score=285, Evalue=5e-77,
Organism=Drosophila melanogaster, GI24580537, Length=420, Percent_Identity=43.8095238095238, Blast_Score=285, Evalue=5e-77,
Organism=Drosophila melanogaster, GI24580533, Length=420, Percent_Identity=43.8095238095238, Blast_Score=285, Evalue=5e-77,
Organism=Drosophila melanogaster, GI24580535, Length=420, Percent_Identity=43.8095238095238, Blast_Score=285, Evalue=5e-77,
Organism=Drosophila melanogaster, GI24580531, Length=420, Percent_Identity=43.8095238095238, Blast_Score=285, Evalue=5e-77,
Organism=Drosophila melanogaster, GI24580541, Length=405, Percent_Identity=43.2098765432099, Blast_Score=265, Evalue=3e-71,
Organism=Drosophila melanogaster, GI24580539, Length=150, Percent_Identity=37.3333333333333, Blast_Score=89, Evalue=7e-18,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 45328; Mature: 45328

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRANYLFTSESVAEGHPDKVCDRISDEIVDLIYREAAKTGVDPWTVRIACETLATTNRVV
CCCCEEECCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHCCCCEEE
IAGEVRVPDTLLKKDKDGKVVKDAAGHPVINPSKFKSAARKAIRDIGYEQDGFHWKTAKI
EEECCCCCHHHHCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
DVLLHPQSADIAQGVDNASDKQGDEGAGDQGIMFGYACKETPDLMPAPIYYSHRILQLLA
EEEECCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
TARKSGEGEAAKLGPDAKSQVTVRYVDGKASEAVSIVLSTQHLDASWDSKKVREVVEPYI
HHHHCCCCCCEECCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHH
REALGELKIADDCQWYINPTGKFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDT
HHHHHCCEECCCCEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCC
TKVDRSAAYAARYLAKNVVAAGLAERCTIQIAYAIGVAQPLSIYVDLHGTGKVSEDQVEA
CHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEEEECCCCCEEEEEEECCCCCCCHHHHHH
AIRKVMDLSPSGIRRHLDLNKPIYAKTSSYGHFGRKAGRDGSFSWEKLDLVKPLKEALSA
HHHHHHCCCHHHHHHHCCCCCCEEEECCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRANYLFTSESVAEGHPDKVCDRISDEIVDLIYREAAKTGVDPWTVRIACETLATTNRVV
CCCCEEECCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHCCCCEEE
IAGEVRVPDTLLKKDKDGKVVKDAAGHPVINPSKFKSAARKAIRDIGYEQDGFHWKTAKI
EEECCCCCHHHHCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
DVLLHPQSADIAQGVDNASDKQGDEGAGDQGIMFGYACKETPDLMPAPIYYSHRILQLLA
EEEECCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
TARKSGEGEAAKLGPDAKSQVTVRYVDGKASEAVSIVLSTQHLDASWDSKKVREVVEPYI
HHHHCCCCCCEECCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHH
REALGELKIADDCQWYINPTGKFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDT
HHHHHCCEECCCCEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCC
TKVDRSAAYAARYLAKNVVAAGLAERCTIQIAYAIGVAQPLSIYVDLHGTGKVSEDQVEA
CHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEEEECCCCCEEEEEEECCCCCCCHHHHHH
AIRKVMDLSPSGIRRHLDLNKPIYAKTSSYGHFGRKAGRDGSFSWEKLDLVKPLKEALSA
HHHHHHCCCHHHHHHHCCCCCCEEEECCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA