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The map label for this gene is cysK [H]

Identifier: 15887662

GI number: 15887662

Start: 305093

End: 306061

Strand: Reverse

Name: cysK [H]

Synonym: Atu0312

Alternate gene names: 15887662

Gene position: 306061-305093 (Counterclockwise)

Preceding gene: 159184252

Following gene: 159184247

Centisome position: 10.77

GC content: 60.58

Gene sequence:

>969_bases
ATGCCCGAAGCCAGAAAGCCCGGACGCGGACGCGTCTATTCCTCAATCACGGAAACAATCGGCGACACGCCGATCGTGAG
GCTCGACAAGCTGGCGAAGGAAAAGGGCGTGAAGGCAAACCTGCTCGCCAAGCTGGAGTTCTTCAACCCTATCGGTTCCG
TGAAGGACCGTATCGGCGTTGCCATGATCGAAGCGCTGGAAGCACAGGGTAAGATCACTCCCGGCAAGACGACGCTGGTG
GAGCCGACTTCAGGTAATACCGGCATCGCGCTCGCCTTTGCCGCCGCCGCCAAGGGTTACAAGCTGATCCTCACCATGCC
GGAAACCATGTCGGTTGAACGCAGAAAGATGCTGGCGCTGCTGGGTGCCGAGCTGGTGCTGACCGAAGGCCCGAAGGGCA
TGAAGGGCGCCATTGCCAAGGCTCAGGAACTGGCAGAGACCCTGCCCGACGCCGTCATTCCCCAACAATTCGAAAACCCC
GCCAATCCGGATATTCACCGCAGGACAACGGCGGAAGAAATCTGGAACGACACCGATGGCACGGTCGATATCCTCGTTTC
CGGGATCGGCACCGGCGGCACGATCACCGGCACGGGCCAGGTTCTGAAATCCCGCAAGCCCGAAGTGAAGGTGATTGCGG
TCGAGCCCGCCGACAGCCCGGTTCTTTCCGGCGGCAATCCCGGCCCGCACAAGATTCAGGGCATCGGCGCCGGCTTTGCA
CCCGCCATTCTCGACACCGGCGTCTATGATGAAATCGTCACCGTGACCAATGACGAGGCATTTGAAATTGCGCGTCTGGC
GGCACGGCTCGAAGGCGTGCCGGTCGGCATCTCCTCGGGTGCGGCACTGGCCGCGGCCATCAAGGTCGGCGCGCGTGAGG
AAAATGCCGGCAAGAACATCGTCGTCATCATTCCTTCTTTCGCAGAACGTTATCTTTCGACCGCGCTTTTCGAAGGCCTT
GGGCTTTAA

Upstream 100 bases:

>100_bases
CCGGTCATTTCGCGATATTAGGACGAACGTTCGCTAGCCTGAGAGGGCTATTGCTCCTATTCTGCCGCTCGAACTTGCGG
TCTTCATACAGGAGAACATC

Downstream 100 bases:

>100_bases
GTCAGGTTCTCGCGCACGGTGCTTATGCACCGGTCAATTGATATCCCGATGTGCCGCCCGCCCCGCTGGCGGCACATCGT
TTTTTTGCGGTTAAGGCCCG

Product: cysteine synthase

Products: NA

Alternate protein names: OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MPEARKPGRGRVYSSITETIGDTPIVRLDKLAKEKGVKANLLAKLEFFNPIGSVKDRIGVAMIEALEAQGKITPGKTTLV
EPTSGNTGIALAFAAAAKGYKLILTMPETMSVERRKMLALLGAELVLTEGPKGMKGAIAKAQELAETLPDAVIPQQFENP
ANPDIHRRTTAEEIWNDTDGTVDILVSGIGTGGTITGTGQVLKSRKPEVKVIAVEPADSPVLSGGNPGPHKIQGIGAGFA
PAILDTGVYDEIVTVTNDEAFEIARLAARLEGVPVGISSGAALAAAIKVGAREENAGKNIVVIIPSFAERYLSTALFEGL
GL

Sequences:

>Translated_322_residues
MPEARKPGRGRVYSSITETIGDTPIVRLDKLAKEKGVKANLLAKLEFFNPIGSVKDRIGVAMIEALEAQGKITPGKTTLV
EPTSGNTGIALAFAAAAKGYKLILTMPETMSVERRKMLALLGAELVLTEGPKGMKGAIAKAQELAETLPDAVIPQQFENP
ANPDIHRRTTAEEIWNDTDGTVDILVSGIGTGGTITGTGQVLKSRKPEVKVIAVEPADSPVLSGGNPGPHKIQGIGAGFA
PAILDTGVYDEIVTVTNDEAFEIARLAARLEGVPVGISSGAALAAAIKVGAREENAGKNIVVIIPSFAERYLSTALFEGL
GL
>Mature_321_residues
PEARKPGRGRVYSSITETIGDTPIVRLDKLAKEKGVKANLLAKLEFFNPIGSVKDRIGVAMIEALEAQGKITPGKTTLVE
PTSGNTGIALAFAAAAKGYKLILTMPETMSVERRKMLALLGAELVLTEGPKGMKGAIAKAQELAETLPDAVIPQQFENPA
NPDIHRRTTAEEIWNDTDGTVDILVSGIGTGGTITGTGQVLKSRKPEVKVIAVEPADSPVLSGGNPGPHKIQGIGAGFAP
AILDTGVYDEIVTVTNDEAFEIARLAARLEGVPVGISSGAALAAAIKVGAREENAGKNIVVIIPSFAERYLSTALFEGLG
L

Specific function: Catalyzes the conversion of O-acetylserine (OAS) to cysteine through the elimination of acetate and addition of hydrogen sulfide [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=317, Percent_Identity=41.9558359621451, Blast_Score=235, Evalue=5e-62,
Organism=Homo sapiens, GI295821200, Length=317, Percent_Identity=41.9558359621451, Blast_Score=235, Evalue=5e-62,
Organism=Homo sapiens, GI4557415, Length=317, Percent_Identity=41.9558359621451, Blast_Score=235, Evalue=5e-62,
Organism=Escherichia coli, GI1788754, Length=319, Percent_Identity=55.1724137931034, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI2367138, Length=304, Percent_Identity=39.1447368421053, Blast_Score=199, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI115535073, Length=298, Percent_Identity=55.3691275167785, Blast_Score=304, Evalue=4e-83,
Organism=Caenorhabditis elegans, GI17535051, Length=303, Percent_Identity=50.1650165016502, Blast_Score=303, Evalue=7e-83,
Organism=Caenorhabditis elegans, GI17562970, Length=300, Percent_Identity=49.3333333333333, Blast_Score=290, Evalue=5e-79,
Organism=Caenorhabditis elegans, GI32566674, Length=295, Percent_Identity=49.8305084745763, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17561720, Length=303, Percent_Identity=47.8547854785479, Blast_Score=256, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI32566672, Length=212, Percent_Identity=48.5849056603774, Blast_Score=208, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17534315, Length=325, Percent_Identity=40.9230769230769, Blast_Score=199, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI25147552, Length=322, Percent_Identity=39.1304347826087, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17561716, Length=121, Percent_Identity=44.6280991735537, Blast_Score=102, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6321594, Length=317, Percent_Identity=36.9085173501577, Blast_Score=195, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6321449, Length=327, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24643623, Length=316, Percent_Identity=37.9746835443038, Blast_Score=197, Evalue=7e-51,
Organism=Drosophila melanogaster, GI20129101, Length=316, Percent_Identity=37.9746835443038, Blast_Score=197, Evalue=7e-51,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 33623; Mature: 33492

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEARKPGRGRVYSSITETIGDTPIVRLDKLAKEKGVKANLLAKLEFFNPIGSVKDRIGV
CCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHEEEEHHHCCHHHHHHHHHH
AMIEALEAQGKITPGKTTLVEPTSGNTGIALAFAAAAKGYKLILTMPETMSVERRKMLAL
HHHHHHHHCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCEEEEECCCHHHHHHHHHHHH
LGAELVLTEGPKGMKGAIAKAQELAETLPDAVIPQQFENPANPDIHRRTTAEEIWNDTDG
HCCEEEEECCCCCHHHHHHHHHHHHHHCCHHCCCHHHCCCCCCCCHHHCCHHHHHCCCCC
TVDILVSGIGTGGTITGTGQVLKSRKPEVKVIAVEPADSPVLSGGNPGPHKIQGIGAGFA
EEEEEEECCCCCCEECCCHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCH
PAILDTGVYDEIVTVTNDEAFEIARLAARLEGVPVGISSGAALAAAIKVGAREENAGKNI
HHHHCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCEE
VVIIPSFAERYLSTALFEGLGL
EEEECHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PEARKPGRGRVYSSITETIGDTPIVRLDKLAKEKGVKANLLAKLEFFNPIGSVKDRIGV
CCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHEEEEHHHCCHHHHHHHHHH
AMIEALEAQGKITPGKTTLVEPTSGNTGIALAFAAAAKGYKLILTMPETMSVERRKMLAL
HHHHHHHHCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCEEEEECCCHHHHHHHHHHHH
LGAELVLTEGPKGMKGAIAKAQELAETLPDAVIPQQFENPANPDIHRRTTAEEIWNDTDG
HCCEEEEECCCCCHHHHHHHHHHHHHHCCHHCCCHHHCCCCCCCCHHHCCHHHHHCCCCC
TVDILVSGIGTGGTITGTGQVLKSRKPEVKVIAVEPADSPVLSGGNPGPHKIQGIGAGFA
EEEEEEECCCCCCEECCCHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCH
PAILDTGVYDEIVTVTNDEAFEIARLAARLEGVPVGISSGAALAAAIKVGAREENAGKNI
HHHHCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCEE
VVIIPSFAERYLSTALFEGLGL
EEEECHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]