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The map label for this gene is lepA [H]

Identifier: 15887591

GI number: 15887591

Start: 240039

End: 241865

Strand: Reverse

Name: lepA [H]

Synonym: Atu0241

Alternate gene names: 15887591

Gene position: 241865-240039 (Counterclockwise)

Preceding gene: 15887592

Following gene: 159184226

Centisome position: 8.51

GC content: 59.55

Gene sequence:

>1827_bases
ATGAGCACAAATTCGACCCGCACGCCCCTGGACCATATCCGCAACTTCTCGATCGTTGCCCACATCGATCACGGCAAATC
GACGCTGGCCGACCGGTTGATCCAGTCGACGGGCGGCCTTGCCGAGCGCGACATGTCGGAACAGGTTCTCGATTCGATGG
ATATCGAGCGCGAGCGCGGCATCACCATCAAGGCCCAGACCGTACGCCTGCACTACAAGGCGAATAACGGCGAGACCTAT
GTGCTAAACCTCATCGACACGCCCGGTCATGTCGACTTTGCCTACGAAGTATCCCGCTCGCTTTCAGCCTGCGAAGGTTC
CCTGCTGGTGGTTGACGCATCGCAGGGCGTGGAAGCCCAGACGCTCGCCAACGTTTATCAGGCGATCGACAATAATCACG
AGCTGGTCACGGTCTTGAACAAGATCGACCTGCCGGCGGCCGAACCAGAACGCATCAAGGAACAGATCGAGGAAGTGATC
GGTATCGACGCCTCCGACGCCGTGATGATCTCGGCCAAGACCGGTCTTGGCATTCCCGATGTTCTGGAAGCCATTGTCAA
CCGTTTGCCGCCGCCGAAAAGCGATGTCGGCGAAAACGGGCCGCTGAAGGCACTGCTGGTCGACAGCTGGTACGACACCT
ATTTGGGCGTCATGGTTCTGGTGCGCGTCATCGACGGCGTGCTGACCAAGGGCCAGCAGATCCGCATGATGGGTTCCGGC
GCGAAATACGGCGTCGAGCGCGTTGGCGTACTTACCCCGAAGATGGTCAATGTCGATAGCCTCGGCCCCGGCGAGATCGG
CTTCATCACCGCCTCGATCAAGGAAGTCGCCGATACCCGCGTTGGCGACACGATCACCGACGACAAGCGCCCGACGGCGC
AAGCCCTGCCCGGATTCAAGCCCGCGCAGCCGGTGGTATTCTGTGGTCTCTTCCCGGTCGATGCGGCAGACTTCGAAGAC
CTGCGCGCGGCTGTCGGCAAACTTCGCCTCAACGACGCTTCCTTCTCCTTCGAAATGGAATCGTCAGCCGCCCTCGGTTT
CGGTTTCCGCTGCGGCTTCCTCGGCCTGCTGCATCTCGAAATCATCCAGGAACGCCTTGAGCGCGAGTTCAATCTCGATC
TCGTCGCCACCGCCCCTTCGGTTGTCTATGAAATGACGCTGACGGATGGCACGGAAAAGGAACTTCACAACCCGGCAGAT
ATGCCTGACGTGGTGAAGATCAAGGAAATCCGCGAGCCGTGGATCAAGGCGACGATCCTGACGCCGGATGAATATCTCGG
CGGCATCCTGAAGCTCTGTCAGGACCGGCGCGGCCTGCAGACGGAGCTGACCTATGTCGGCAACCGCGCGATGATCACCT
ATGAACTGCCGCTCAACGAAGTGGTGTTCGATTTCTACGACCGCCTGAAATCCATCTCCAAGGGTTACGCCTCGTTCGAT
TACAACATCATCGACTATCGCGAAGGCGATCTCGTCAAGATGTCGATCCTCGTCAATGGCGATCCTGTCGATGCCCTCTC
CATGCTGGTGCACCGCTCTGCGGCCGACCGGCGCGGGCGCGGCATGTGCGAAAAGCTGAAGGAACTCATTCCGCCGCACA
TGTTCCAGATCCCCATCCAGGCGGCCATCGGCGGCAAGGTCATCGCCCGCGAAACCGTGCGCGCGCTGCGCAAGGACGTG
ACGGCGAAGTGTTACGGCGGTGACGCAACGCGCAAGCGCAAGCTTCTGGACAAACAGAAGGAAGGCAAAAAGCGCATGCG
CCAGTTCGGCAAGGTGGAAATTCCGCAGGAAGCCTTTATCGCCGCTCTGAAGATGAACGACGAATAA

Upstream 100 bases:

>100_bases
CGACTGGCCAGAGCAATTTGCGGTCTGAAAGCCCATTTTAAAGGGCTTTTAACCAAATTCCCGCTTTGCGGCATGACAAG
CCCCTGCTATAGCGCGGAAC

Downstream 100 bases:

>100_bases
GCGGCAATCACTGCATCGCTGTTAAAGGGAGGCCCACAGCCTCCCTTTTGCGTTTGAAGGCCGATCCTCAAGCGTGCAGC
TTCGCACCCTTGGTGATCTT

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 608; Mature: 607

Protein sequence:

>608_residues
MSTNSTRTPLDHIRNFSIVAHIDHGKSTLADRLIQSTGGLAERDMSEQVLDSMDIERERGITIKAQTVRLHYKANNGETY
VLNLIDTPGHVDFAYEVSRSLSACEGSLLVVDASQGVEAQTLANVYQAIDNNHELVTVLNKIDLPAAEPERIKEQIEEVI
GIDASDAVMISAKTGLGIPDVLEAIVNRLPPPKSDVGENGPLKALLVDSWYDTYLGVMVLVRVIDGVLTKGQQIRMMGSG
AKYGVERVGVLTPKMVNVDSLGPGEIGFITASIKEVADTRVGDTITDDKRPTAQALPGFKPAQPVVFCGLFPVDAADFED
LRAAVGKLRLNDASFSFEMESSAALGFGFRCGFLGLLHLEIIQERLEREFNLDLVATAPSVVYEMTLTDGTEKELHNPAD
MPDVVKIKEIREPWIKATILTPDEYLGGILKLCQDRRGLQTELTYVGNRAMITYELPLNEVVFDFYDRLKSISKGYASFD
YNIIDYREGDLVKMSILVNGDPVDALSMLVHRSAADRRGRGMCEKLKELIPPHMFQIPIQAAIGGKVIARETVRALRKDV
TAKCYGGDATRKRKLLDKQKEGKKRMRQFGKVEIPQEAFIAALKMNDE

Sequences:

>Translated_608_residues
MSTNSTRTPLDHIRNFSIVAHIDHGKSTLADRLIQSTGGLAERDMSEQVLDSMDIERERGITIKAQTVRLHYKANNGETY
VLNLIDTPGHVDFAYEVSRSLSACEGSLLVVDASQGVEAQTLANVYQAIDNNHELVTVLNKIDLPAAEPERIKEQIEEVI
GIDASDAVMISAKTGLGIPDVLEAIVNRLPPPKSDVGENGPLKALLVDSWYDTYLGVMVLVRVIDGVLTKGQQIRMMGSG
AKYGVERVGVLTPKMVNVDSLGPGEIGFITASIKEVADTRVGDTITDDKRPTAQALPGFKPAQPVVFCGLFPVDAADFED
LRAAVGKLRLNDASFSFEMESSAALGFGFRCGFLGLLHLEIIQERLEREFNLDLVATAPSVVYEMTLTDGTEKELHNPAD
MPDVVKIKEIREPWIKATILTPDEYLGGILKLCQDRRGLQTELTYVGNRAMITYELPLNEVVFDFYDRLKSISKGYASFD
YNIIDYREGDLVKMSILVNGDPVDALSMLVHRSAADRRGRGMCEKLKELIPPHMFQIPIQAAIGGKVIARETVRALRKDV
TAKCYGGDATRKRKLLDKQKEGKKRMRQFGKVEIPQEAFIAALKMNDE
>Mature_607_residues
STNSTRTPLDHIRNFSIVAHIDHGKSTLADRLIQSTGGLAERDMSEQVLDSMDIERERGITIKAQTVRLHYKANNGETYV
LNLIDTPGHVDFAYEVSRSLSACEGSLLVVDASQGVEAQTLANVYQAIDNNHELVTVLNKIDLPAAEPERIKEQIEEVIG
IDASDAVMISAKTGLGIPDVLEAIVNRLPPPKSDVGENGPLKALLVDSWYDTYLGVMVLVRVIDGVLTKGQQIRMMGSGA
KYGVERVGVLTPKMVNVDSLGPGEIGFITASIKEVADTRVGDTITDDKRPTAQALPGFKPAQPVVFCGLFPVDAADFEDL
RAAVGKLRLNDASFSFEMESSAALGFGFRCGFLGLLHLEIIQERLEREFNLDLVATAPSVVYEMTLTDGTEKELHNPADM
PDVVKIKEIREPWIKATILTPDEYLGGILKLCQDRRGLQTELTYVGNRAMITYELPLNEVVFDFYDRLKSISKGYASFDY
NIIDYREGDLVKMSILVNGDPVDALSMLVHRSAADRRGRGMCEKLKELIPPHMFQIPIQAAIGGKVIARETVRALRKDVT
AKCYGGDATRKRKLLDKQKEGKKRMRQFGKVEIPQEAFIAALKMNDE

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=608, Percent_Identity=50.328947368421, Blast_Score=629, Evalue=1e-180,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=48.8721804511278, Blast_Score=125, Evalue=2e-28,
Organism=Homo sapiens, GI18390331, Length=169, Percent_Identity=37.8698224852071, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI25306283, Length=153, Percent_Identity=44.4444444444444, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI19923640, Length=153, Percent_Identity=44.4444444444444, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=40.2777777777778, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI25306287, Length=153, Percent_Identity=44.4444444444444, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI310132016, Length=110, Percent_Identity=45.4545454545455, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI310110807, Length=110, Percent_Identity=45.4545454545455, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI310123363, Length=110, Percent_Identity=45.4545454545455, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI217272894, Length=133, Percent_Identity=33.0827067669173, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI217272892, Length=133, Percent_Identity=33.0827067669173, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI94966752, Length=97, Percent_Identity=42.2680412371134, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI53729339, Length=226, Percent_Identity=27.8761061946903, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI53729337, Length=226, Percent_Identity=27.8761061946903, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788922, Length=596, Percent_Identity=58.3892617449664, Blast_Score=711, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=508, Percent_Identity=27.1653543307087, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1789738, Length=161, Percent_Identity=34.7826086956522, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1790835, Length=158, Percent_Identity=31.6455696202532, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1789559, Length=222, Percent_Identity=28.3783783783784, Blast_Score=76, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=620, Percent_Identity=38.5483870967742, Blast_Score=454, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI17556745, Length=206, Percent_Identity=33.9805825242718, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71988811, Length=136, Percent_Identity=39.7058823529412, Blast_Score=105, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71988819, Length=136, Percent_Identity=39.7058823529412, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17533571, Length=147, Percent_Identity=36.734693877551, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=156, Percent_Identity=37.8205128205128, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=35.3383458646617, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71994658, Length=221, Percent_Identity=27.1493212669683, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=269, Percent_Identity=27.1375464684015, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=605, Percent_Identity=44.297520661157, Blast_Score=538, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6323098, Length=200, Percent_Identity=35, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6324707, Length=162, Percent_Identity=37.037037037037, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6320593, Length=162, Percent_Identity=37.037037037037, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6322359, Length=120, Percent_Identity=39.1666666666667, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324166, Length=170, Percent_Identity=34.7058823529412, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324761, Length=255, Percent_Identity=26.6666666666667, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI78706572, Length=605, Percent_Identity=45.6198347107438, Blast_Score=554, Evalue=1e-158,
Organism=Drosophila melanogaster, GI24582462, Length=167, Percent_Identity=38.3233532934132, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI28574573, Length=173, Percent_Identity=40.4624277456647, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24585711, Length=148, Percent_Identity=36.4864864864865, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585713, Length=148, Percent_Identity=36.4864864864865, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585709, Length=148, Percent_Identity=36.4864864864865, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21357743, Length=161, Percent_Identity=35.4037267080745, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI221458488, Length=158, Percent_Identity=37.9746835443038, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19921738, Length=220, Percent_Identity=28.6363636363636, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 67124; Mature: 66993

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTNSTRTPLDHIRNFSIVAHIDHGKSTLADRLIQSTGGLAERDMSEQVLDSMDIERERG
CCCCCCCCCHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHCC
ITIKAQTVRLHYKANNGETYVLNLIDTPGHVDFAYEVSRSLSACEGSLLVVDASQGVEAQ
CEEEEEEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHH
TLANVYQAIDNNHELVTVLNKIDLPAAEPERIKEQIEEVIGIDASDAVMISAKTGLGIPD
HHHHHHHHHCCCCCEEEEEHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHH
VLEAIVNRLPPPKSDVGENGPLKALLVDSWYDTYLGVMVLVRVIDGVLTKGQQIRMMGSG
HHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
AKYGVERVGVLTPKMVNVDSLGPGEIGFITASIKEVADTRVGDTITDDKRPTAQALPGFK
CHHCHHHHCCCCCCEECCCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCHHHHCCCCC
PAQPVVFCGLFPVDAADFEDLRAAVGKLRLNDASFSFEMESSAALGFGFRCGFLGLLHLE
CCCCEEEEEECCCCCCCHHHHHHHHHHEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHH
IIQERLEREFNLDLVATAPSVVYEMTLTDGTEKELHNPADMPDVVKIKEIREPWIKATIL
HHHHHHHHHCCCEEEEECCCEEEEEEECCCCHHHCCCCCCCCHHHHHHHHHCCHHEEEEE
TPDEYLGGILKLCQDRRGLQTELTYVGNRAMITYELPLNEVVFDFYDRLKSISKGYASFD
CCHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCC
YNIIDYREGDLVKMSILVNGDPVDALSMLVHRSAADRRGRGMCEKLKELIPPHMFQIPIQ
CEEEEECCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEHH
AAIGGKVIARETVRALRKDVTAKCYGGDATRKRKLLDKQKEGKKRMRQFGKVEIPQEAFI
HHCCCHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHE
AALKMNDE
EEEECCCC
>Mature Secondary Structure 
STNSTRTPLDHIRNFSIVAHIDHGKSTLADRLIQSTGGLAERDMSEQVLDSMDIERERG
CCCCCCCCHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHCC
ITIKAQTVRLHYKANNGETYVLNLIDTPGHVDFAYEVSRSLSACEGSLLVVDASQGVEAQ
CEEEEEEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHH
TLANVYQAIDNNHELVTVLNKIDLPAAEPERIKEQIEEVIGIDASDAVMISAKTGLGIPD
HHHHHHHHHCCCCCEEEEEHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHH
VLEAIVNRLPPPKSDVGENGPLKALLVDSWYDTYLGVMVLVRVIDGVLTKGQQIRMMGSG
HHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
AKYGVERVGVLTPKMVNVDSLGPGEIGFITASIKEVADTRVGDTITDDKRPTAQALPGFK
CHHCHHHHCCCCCCEECCCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCHHHHCCCCC
PAQPVVFCGLFPVDAADFEDLRAAVGKLRLNDASFSFEMESSAALGFGFRCGFLGLLHLE
CCCCEEEEEECCCCCCCHHHHHHHHHHEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHH
IIQERLEREFNLDLVATAPSVVYEMTLTDGTEKELHNPADMPDVVKIKEIREPWIKATIL
HHHHHHHHHCCCEEEEECCCEEEEEEECCCCHHHCCCCCCCCHHHHHHHHHCCHHEEEEE
TPDEYLGGILKLCQDRRGLQTELTYVGNRAMITYELPLNEVVFDFYDRLKSISKGYASFD
CCHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCC
YNIIDYREGDLVKMSILVNGDPVDALSMLVHRSAADRRGRGMCEKLKELIPPHMFQIPIQ
CEEEEECCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEHH
AAIGGKVIARETVRALRKDVTAKCYGGDATRKRKLLDKQKEGKKRMRQFGKVEIPQEAFI
HHCCCHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHE
AALKMNDE
EEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA