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The map label for this gene is phnE [H]

Identifier: 15887525

GI number: 15887525

Start: 175086

End: 176435

Strand: Reverse

Name: phnE [H]

Synonym: Atu0171

Alternate gene names: 15887525

Gene position: 176435-175086 (Counterclockwise)

Preceding gene: 159184196

Following gene: 159184195

Centisome position: 6.21

GC content: 59.85

Gene sequence:

>1350_bases
ATGTCGTCCAGCTTCATTCTCAACACCGCCGAGCGCGAGAGGCTGACGGCTGCACATCGCCATGTGTTCAACCGCAGTTT
CATGCAACGATATGGTCTGCTCGTCGGCCTTCTCGTCGCCACCGCCTATCTCATCGGCTGCTTTTTCTTCTTCAATGTCG
GCCCGGCTTTCATGCAGGGCCGGTGGGATCGCGCTTCCAGCTATATTCAGGACTGGTATTCCTGGCGTGCCCAGCCGCGC
CTGCGTTTCGAAGACGGCAAGGTCGCGCCGCAATGGTCGAGCCGCGGGCAATATCCGGCCGATGCGAAGATCGACTGGCT
GCAGCCCCTGCCGGACGGCGGTTACAGCGTCGTTTATGCGGGCACACAAAACCGCCTCGATATAACGCCGACCCGGGTGG
ATGTTTATGTCGACGGCAAGAACTACCCCGTCATCATCAACGACGATGCTGTTGTCGCGCCGCAGGGCCTGCCCGACGAA
ATCCAGCAGGACGGCAAGAAGGTTCTCGTGCATTACGGTTTTGCCGGCCAGGCGGAAATCCGCACCAGCCAGGTCTATGT
ACAGCGCCGCTTCCTTGGATGGGCGAACTTCCTGTTCGACACCAAATCTGAATTCTGGGGCAAGGGTTATGGCGAGCTTG
CGGCTCTCGCGCTCTGGGGAGAGCGGCTCGATCCCGCGCGCTCCAATATCGGCCATATGGTCGATGATTTCCTCGACAAC
AGCGTCTGGCAACATGCCGATATCCTGTCCAAGCTGATGCAGACGCTGGTCATGGCATTTGTCGGCACGCTGTTCGGCAC
GCTTGTCGCCCTGCCGCTCGCCTTCATCGCAGCGCGCAACATCACCGCCAACAGGGCGGCAAACTGGGGCATGAAGCGCC
TGTTCGATTTCCTGCGTTCCATCGACATGCTGATCTGGGCGCTGTTCTTCACCCGCAGTTTCGGCCCGGGACCGATCCCG
GGAATTGCCGCGATCTTCTTCACCGATACCGGCGCGCTCGGCAAGGTCTATGCCGAGGCGCTGGAAAATGTCGACGACAA
GCAGCGCGAGGGCGTCAAGTCGGTCGGCGCATCCCCGATTGCGGTCAACCGCTTCGGCGTGTTGCCGCAGGTGTTGCCGG
TCTTCATTTCCCAATCGCTGTATTTCTGGGAAAGCAATACCCGCTCCGCCACGATCATCGGTGCGGTGGGTGCCGGCGGC
ATCGGCCTGAAACTGCTGGAAGCGATGGGTACCAATGCCGACTGGGACAAGGTCGCCTATATGGTCCTGCTCATCCTCTT
CGTCGTCTTCCTGTTCGACCATATTTCCAATTCGCTGCGTTCGCGCCTGATCGGTAAAACACAGCACTAG

Upstream 100 bases:

>100_bases
TTCTGCTGATCACCATCATCGCCGTCGACCAGTTCTCCAGCTGGCTGCGCCGCAGACTGATCGGCCAACAATCCTTCGAA
TTCGGACGGGGAGCGGCCTG

Downstream 100 bases:

>100_bases
GCCCAAGCGGGCGGGATAACGAAAGTCGCGATGACGTTCGTTATCCCGCACCGCGCACGCTCACGCAGTCCTGGTTTGCG
CTGGCCATATCTCCTCACCA

Product: ABC transporter, membrane spanning protein (phosphonate)

Products: 3-phospho-serine [Cytoplasm]; phosphite [Cytoplasm]; ADP; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSSSFILNTAERERLTAAHRHVFNRSFMQRYGLLVGLLVATAYLIGCFFFFNVGPAFMQGRWDRASSYIQDWYSWRAQPR
LRFEDGKVAPQWSSRGQYPADAKIDWLQPLPDGGYSVVYAGTQNRLDITPTRVDVYVDGKNYPVIINDDAVVAPQGLPDE
IQQDGKKVLVHYGFAGQAEIRTSQVYVQRRFLGWANFLFDTKSEFWGKGYGELAALALWGERLDPARSNIGHMVDDFLDN
SVWQHADILSKLMQTLVMAFVGTLFGTLVALPLAFIAARNITANRAANWGMKRLFDFLRSIDMLIWALFFTRSFGPGPIP
GIAAIFFTDTGALGKVYAEALENVDDKQREGVKSVGASPIAVNRFGVLPQVLPVFISQSLYFWESNTRSATIIGAVGAGG
IGLKLLEAMGTNADWDKVAYMVLLILFVVFLFDHISNSLRSRLIGKTQH

Sequences:

>Translated_449_residues
MSSSFILNTAERERLTAAHRHVFNRSFMQRYGLLVGLLVATAYLIGCFFFFNVGPAFMQGRWDRASSYIQDWYSWRAQPR
LRFEDGKVAPQWSSRGQYPADAKIDWLQPLPDGGYSVVYAGTQNRLDITPTRVDVYVDGKNYPVIINDDAVVAPQGLPDE
IQQDGKKVLVHYGFAGQAEIRTSQVYVQRRFLGWANFLFDTKSEFWGKGYGELAALALWGERLDPARSNIGHMVDDFLDN
SVWQHADILSKLMQTLVMAFVGTLFGTLVALPLAFIAARNITANRAANWGMKRLFDFLRSIDMLIWALFFTRSFGPGPIP
GIAAIFFTDTGALGKVYAEALENVDDKQREGVKSVGASPIAVNRFGVLPQVLPVFISQSLYFWESNTRSATIIGAVGAGG
IGLKLLEAMGTNADWDKVAYMVLLILFVVFLFDHISNSLRSRLIGKTQH
>Mature_448_residues
SSSFILNTAERERLTAAHRHVFNRSFMQRYGLLVGLLVATAYLIGCFFFFNVGPAFMQGRWDRASSYIQDWYSWRAQPRL
RFEDGKVAPQWSSRGQYPADAKIDWLQPLPDGGYSVVYAGTQNRLDITPTRVDVYVDGKNYPVIINDDAVVAPQGLPDEI
QQDGKKVLVHYGFAGQAEIRTSQVYVQRRFLGWANFLFDTKSEFWGKGYGELAALALWGERLDPARSNIGHMVDDFLDNS
VWQHADILSKLMQTLVMAFVGTLFGTLVALPLAFIAARNITANRAANWGMKRLFDFLRSIDMLIWALFFTRSFGPGPIPG
IAAIFFTDTGALGKVYAEALENVDDKQREGVKSVGASPIAVNRFGVLPQVLPVFISQSLYFWESNTRSATIIGAVGAGGI
GLKLLEAMGTNADWDKVAYMVLLILFVVFLFDHISNSLRSRLIGKTQH

Specific function: Part of the binding-protein-dependent transport system for phosphonates; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG3639

COG function: function code P; ABC-type phosphate/phosphonate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005769 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 50198; Mature: 50067

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSFILNTAERERLTAAHRHVFNRSFMQRYGLLVGLLVATAYLIGCFFFFNVGPAFMQG
CCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCC
RWDRASSYIQDWYSWRAQPRLRFEDGKVAPQWSSRGQYPADAKIDWLQPLPDGGYSVVYA
CHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
GTQNRLDITPTRVDVYVDGKNYPVIINDDAVVAPQGLPDEIQQDGKKVLVHYGFAGQAEI
CCCCCEECCCCEEEEEECCCCCCEEECCCCEECCCCCCHHHHCCCCEEEEEECCCCCCHH
RTSQVYVQRRFLGWANFLFDTKSEFWGKGYGELAALALWGERLDPARSNIGHMVDDFLDN
HHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
SVWQHADILSKLMQTLVMAFVGTLFGTLVALPLAFIAARNITANRAANWGMKRLFDFLRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHH
IDMLIWALFFTRSFGPGPIPGIAAIFFTDTGALGKVYAEALENVDDKQREGVKSVGASPI
HHHHHHHHHHHCCCCCCCCCHHEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCE
AVNRFGVLPQVLPVFISQSLYFWESNTRSATIIGAVGAGGIGLKLLEAMGTNADWDKVAY
EEHHHCCHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHCCCCCHHHHHH
MVLLILFVVFLFDHISNSLRSRLIGKTQH
HHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SSSFILNTAERERLTAAHRHVFNRSFMQRYGLLVGLLVATAYLIGCFFFFNVGPAFMQG
CCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCC
RWDRASSYIQDWYSWRAQPRLRFEDGKVAPQWSSRGQYPADAKIDWLQPLPDGGYSVVYA
CHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
GTQNRLDITPTRVDVYVDGKNYPVIINDDAVVAPQGLPDEIQQDGKKVLVHYGFAGQAEI
CCCCCEECCCCEEEEEECCCCCCEEECCCCEECCCCCCHHHHCCCCEEEEEECCCCCCHH
RTSQVYVQRRFLGWANFLFDTKSEFWGKGYGELAALALWGERLDPARSNIGHMVDDFLDN
HHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
SVWQHADILSKLMQTLVMAFVGTLFGTLVALPLAFIAARNITANRAANWGMKRLFDFLRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHH
IDMLIWALFFTRSFGPGPIPGIAAIFFTDTGALGKVYAEALENVDDKQREGVKSVGASPI
HHHHHHHHHHHCCCCCCCCCHHEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCE
AVNRFGVLPQVLPVFISQSLYFWESNTRSATIIGAVGAGGIGLKLLEAMGTNADWDKVAY
EEHHHCCHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHCCCCCHHHHHH
MVLLILFVVFLFDHISNSLRSRLIGKTQH
HHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]