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The map label for this gene is glcB [H]

Identifier: 15887405

GI number: 15887405

Start: 47736

End: 49970

Strand: Direct

Name: glcB [H]

Synonym: Atu0047

Alternate gene names: 15887405

Gene position: 47736-49970 (Clockwise)

Preceding gene: 15887404

Following gene: 159184140

Centisome position: 1.68

GC content: 59.42

Gene sequence:

>2235_bases
ATGCCGCGATTGACGAGCGACCGCCTCAGCGTCCAATCTATGCCTTCAGAATATAAGGAGGCGCATGTGAGCCGCACGGA
TAAATTCGGTCTTTCCATTGACGACAGGCTGTATGCCTTTTTGACGGACGAGGTGCTTCCGGGCACAGGTCTGGACAGCG
AGACCTTCTTTGAGGGTTTTTCGGCCATCGTCCATGAGCTTTCTCCGAAGAATCGTGAACTGCTTGCCAAGCGCGATGCG
CTGCAGGAAAAAATCGATGGTTGGTACCGTGAGAACGGCGCTCCCTCGGATTTCGATGCCTATGAGGCCTTCCTGAAGGA
GATCGGCTACCTGCTGCCGGAAGGTCCCGGCTTCAAGGTCGAAACTAATAATGTCGACCCGGAAATTGCCGTCGTCGCCG
GTCCGCAACTCGTTGTTCCCGTCATGAATGCGCGTTATGCGCTGAACGCCGCCAATGCCCGCTGGGGTTCGCTTTATGAT
GCGCTCTACGGCACAGACGCCATTTCCGATGCCGACGGTGCGGAAAAGGGCAGGGGTTACAATCCGAAGCGTGGTGACAA
GGTCATCGCTTGGGCGCGCAATTTCCTCGATGAATCCGCCCCGCTCGAGACAGGAAGCTGGTCCGACGTCACAGGCTTCA
ATATTGCTGACGGCCTGTTGCAGCTTGCCATCGGTGCTGCCACGACTGGCCTCAAGGATGCAGTTCAATTCAAAGGTTTC
AGCGGTGAAGCGGCAAAGCCCGCCACGATCCTGCTCGGCAAGAACGGTCTGCACACGGAAATCGTCATCGATCCCTCGAC
CGAAATCGGCAAAAGCGATAGAGCAGGCATATCGGACGTCATTCTCGAATCAGCGCTGACGACCATCATGGATTGCGAGG
ATTCTGTCGCCGCCGTCGATGCCGAGGACAAGGTGCTGGTTTACGGCAACTGGCTTGGCCTGATGCGCGGCGATTTGACG
GAAGCCGTCTCCAAGGGCGGCAACACTTTCACCCGCCGCCTCAACCCGGATCGTTATTATACCGCTCCCGATGGTTCCGC
GCTCACGCTGCCGGGCCGTTCCCTGATGCTGGTGCGCAATGTCGGTCATCTCATGACCAATCCGGCGATCCTCGACAGGG
ATGGCCGCGACGTGCCGGAAGGCATTATGGATGCCGTCGTCACGGCGCTGATCGCGCTTTACGATGTCGGCCCGTCCGGA
CGACGCCAGAATTCCCGCGCCGGCTCCATGTATGTCGTCAAGCCGAAGATGCACGGACCGGAAGAGGTTGCTTTCGCCAA
CGAGATATTCGCCCGCGTCGAAAATCTTGTGGGCATGGCGCCGAACACCATGAAAATGGGCATCATGGATGAGGAGCGCC
GCACCACCGTCAACCTCAAGGAAAGCATTCGCGCGGCGAAGGATCGCGTAGTCTTCATCAATACCGGCTTCCTCGATCGC
ACCGGCGACGAAATCCATACCTCTATGGAAGCAGGCCCGATGATCCGCAAGGGCGACATGAAACAGGCTGCATGGATCGC
GGCTTATGAAAACTGGAACGTCGATATTGGCCTCGAATGCGGTCTCTCCGGCCACGCCCAGATCGGCAAGGGCATGTGGG
CCATGCCGGATCTGATGGCTGCCATGCTGGAGCAGAAGATCGCGCATCCGAAAGCCGGCGCCAATACCGCCTGGGTGCCG
TCGCCGACGGCGGCGACGTTGCACGCCACGCATTACCATAAGGTCGACGTCGCAGCCGTTCAGGAAGGCCTGAAAAGCCG
CGGTCGCGCAAAGCTCTCCGATATCCTGTCGGTGCCGGTCGCACCGCGTCCCAACTGGACGCCGGAGGAAATCCAGCGCG
AGCTTGATAATAACGCCCAGGGCATTCTCGGTTATGTTGTTCGCTGGGTCGATCAGGGTGTCGGCTGCTCCAAGGTGCCT
GACATCAACAATATTGGCCTGATGGAGGACCGCGCCACGCTGCGCATTTCCGCCCAGCACATGGCGAACTGGCTGCGCCA
CGGCGTGGTGACGGAGGCCCAGATCATCAAGACCATGAAGCGCATGGCCGCGGTAGTGGACACGCAGAATGCCGGCGATC
CGGCCTATCTGCCGATGGCATCGGATTTCGATGGATCGGTGGCTTTCCAGGCTGCTGTCGAGCTGGTGCTGAAGGGCCGT
GAACAGCCGAACGGCTACACCGAACCGGTTCTGCACCGCCGCCGTCTGGAGCTGAAAGCAAAGCAGGCGGGCTGA

Upstream 100 bases:

>100_bases
CTGGCTGGTAAACGACCGCATCAAGGATCTGCAGGCGCTTTTGTAGAAAAAGACTATTATCCGCCATATTGTTTCCAAAA
AGGAAATAATATTTGGCTGG

Downstream 100 bases:

>100_bases
AGCGGCCGCCTGGTATTTCCAGTAAAAGTGCGTAGCGGTTTTACGTCCGGAAATGCTGAAAACCAAAGAGATTGAGCATT
GCAGGTGATCCCGTTTTCAC

Product: malate synthase G

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 744; Mature: 743

Protein sequence:

>744_residues
MPRLTSDRLSVQSMPSEYKEAHVSRTDKFGLSIDDRLYAFLTDEVLPGTGLDSETFFEGFSAIVHELSPKNRELLAKRDA
LQEKIDGWYRENGAPSDFDAYEAFLKEIGYLLPEGPGFKVETNNVDPEIAVVAGPQLVVPVMNARYALNAANARWGSLYD
ALYGTDAISDADGAEKGRGYNPKRGDKVIAWARNFLDESAPLETGSWSDVTGFNIADGLLQLAIGAATTGLKDAVQFKGF
SGEAAKPATILLGKNGLHTEIVIDPSTEIGKSDRAGISDVILESALTTIMDCEDSVAAVDAEDKVLVYGNWLGLMRGDLT
EAVSKGGNTFTRRLNPDRYYTAPDGSALTLPGRSLMLVRNVGHLMTNPAILDRDGRDVPEGIMDAVVTALIALYDVGPSG
RRQNSRAGSMYVVKPKMHGPEEVAFANEIFARVENLVGMAPNTMKMGIMDEERRTTVNLKESIRAAKDRVVFINTGFLDR
TGDEIHTSMEAGPMIRKGDMKQAAWIAAYENWNVDIGLECGLSGHAQIGKGMWAMPDLMAAMLEQKIAHPKAGANTAWVP
SPTAATLHATHYHKVDVAAVQEGLKSRGRAKLSDILSVPVAPRPNWTPEEIQRELDNNAQGILGYVVRWVDQGVGCSKVP
DINNIGLMEDRATLRISAQHMANWLRHGVVTEAQIIKTMKRMAAVVDTQNAGDPAYLPMASDFDGSVAFQAAVELVLKGR
EQPNGYTEPVLHRRRLELKAKQAG

Sequences:

>Translated_744_residues
MPRLTSDRLSVQSMPSEYKEAHVSRTDKFGLSIDDRLYAFLTDEVLPGTGLDSETFFEGFSAIVHELSPKNRELLAKRDA
LQEKIDGWYRENGAPSDFDAYEAFLKEIGYLLPEGPGFKVETNNVDPEIAVVAGPQLVVPVMNARYALNAANARWGSLYD
ALYGTDAISDADGAEKGRGYNPKRGDKVIAWARNFLDESAPLETGSWSDVTGFNIADGLLQLAIGAATTGLKDAVQFKGF
SGEAAKPATILLGKNGLHTEIVIDPSTEIGKSDRAGISDVILESALTTIMDCEDSVAAVDAEDKVLVYGNWLGLMRGDLT
EAVSKGGNTFTRRLNPDRYYTAPDGSALTLPGRSLMLVRNVGHLMTNPAILDRDGRDVPEGIMDAVVTALIALYDVGPSG
RRQNSRAGSMYVVKPKMHGPEEVAFANEIFARVENLVGMAPNTMKMGIMDEERRTTVNLKESIRAAKDRVVFINTGFLDR
TGDEIHTSMEAGPMIRKGDMKQAAWIAAYENWNVDIGLECGLSGHAQIGKGMWAMPDLMAAMLEQKIAHPKAGANTAWVP
SPTAATLHATHYHKVDVAAVQEGLKSRGRAKLSDILSVPVAPRPNWTPEEIQRELDNNAQGILGYVVRWVDQGVGCSKVP
DINNIGLMEDRATLRISAQHMANWLRHGVVTEAQIIKTMKRMAAVVDTQNAGDPAYLPMASDFDGSVAFQAAVELVLKGR
EQPNGYTEPVLHRRRLELKAKQAG
>Mature_743_residues
PRLTSDRLSVQSMPSEYKEAHVSRTDKFGLSIDDRLYAFLTDEVLPGTGLDSETFFEGFSAIVHELSPKNRELLAKRDAL
QEKIDGWYRENGAPSDFDAYEAFLKEIGYLLPEGPGFKVETNNVDPEIAVVAGPQLVVPVMNARYALNAANARWGSLYDA
LYGTDAISDADGAEKGRGYNPKRGDKVIAWARNFLDESAPLETGSWSDVTGFNIADGLLQLAIGAATTGLKDAVQFKGFS
GEAAKPATILLGKNGLHTEIVIDPSTEIGKSDRAGISDVILESALTTIMDCEDSVAAVDAEDKVLVYGNWLGLMRGDLTE
AVSKGGNTFTRRLNPDRYYTAPDGSALTLPGRSLMLVRNVGHLMTNPAILDRDGRDVPEGIMDAVVTALIALYDVGPSGR
RQNSRAGSMYVVKPKMHGPEEVAFANEIFARVENLVGMAPNTMKMGIMDEERRTTVNLKESIRAAKDRVVFINTGFLDRT
GDEIHTSMEAGPMIRKGDMKQAAWIAAYENWNVDIGLECGLSGHAQIGKGMWAMPDLMAAMLEQKIAHPKAGANTAWVPS
PTAATLHATHYHKVDVAAVQEGLKSRGRAKLSDILSVPVAPRPNWTPEEIQRELDNNAQGILGYVVRWVDQGVGCSKVPD
INNIGLMEDRATLRISAQHMANWLRHGVVTEAQIIKTMKRMAAVVDTQNAGDPAYLPMASDFDGSVAFQAAVELVLKGRE
QPNGYTEPVLHRRRLELKAKQAG

Specific function: Accounts For Almost The Entire Malate-Synthesizing Activity In Cells Metabolizing Glyoxylate. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family. GlcB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789348, Length=717, Percent_Identity=60.3905160390516, Blast_Score=887, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR001465
- InterPro:   IPR006253 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 80993; Mature: 80862

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLTSDRLSVQSMPSEYKEAHVSRTDKFGLSIDDRLYAFLTDEVLPGTGLDSETFFEGF
CCCCCCCHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHEEEHHHCCCCCCCCHHHHHHHH
SAIVHELSPKNRELLAKRDALQEKIDGWYRENGAPSDFDAYEAFLKEIGYLLPEGPGFKV
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEE
ETNNVDPEIAVVAGPQLVVPVMNARYALNAANARWGSLYDALYGTDAISDADGAEKGRGY
ECCCCCCCEEEEECCEEEEEECCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
NPKRGDKVIAWARNFLDESAPLETGSWSDVTGFNIADGLLQLAIGAATTGLKDAVQFKGF
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCC
SGEAAKPATILLGKNGLHTEIVIDPSTEIGKSDRAGISDVILESALTTIMDCEDSVAAVD
CCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEC
AEDKVLVYGNWLGLMRGDLTEAVSKGGNTFTRRLNPDRYYTAPDGSALTLPGRSLMLVRN
CCCCEEEEECHHHHHHHHHHHHHHCCCCEEEECCCCCCEEECCCCCEEECCCCCEEEHHH
VGHLMTNPAILDRDGRDVPEGIMDAVVTALIALYDVGPSGRRQNSRAGSMYVVKPKMHGP
HHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCC
EEVAFANEIFARVENLVGMAPNTMKMGIMDEERRTTVNLKESIRAAKDRVVFINTGFLDR
HHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHEECHHHHHHHHHCCEEEEECCCCCC
TGDEIHTSMEAGPMIRKGDMKQAAWIAAYENWNVDIGLECGLSGHAQIGKGMWAMPDLMA
CCHHHHHHHHCCCCEECCCCCCEEEEEEECCCCEEEEEECCCCCCHHHCCCCCHHHHHHH
AMLEQKIAHPKAGANTAWVPSPTAATLHATHYHKVDVAAVQEGLKSRGRAKLSDILSVPV
HHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHCCC
APRPNWTPEEIQRELDNNAQGILGYVVRWVDQGVGCSKVPDINNIGLMEDRATLRISAQH
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEHHH
MANWLRHGVVTEAQIIKTMKRMAAVVDTQNAGDPAYLPMASDFDGSVAFQAAVELVLKGR
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHCC
EQPNGYTEPVLHRRRLELKAKQAG
CCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PRLTSDRLSVQSMPSEYKEAHVSRTDKFGLSIDDRLYAFLTDEVLPGTGLDSETFFEGF
CCCCCCHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHEEEHHHCCCCCCCCHHHHHHHH
SAIVHELSPKNRELLAKRDALQEKIDGWYRENGAPSDFDAYEAFLKEIGYLLPEGPGFKV
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEE
ETNNVDPEIAVVAGPQLVVPVMNARYALNAANARWGSLYDALYGTDAISDADGAEKGRGY
ECCCCCCCEEEEECCEEEEEECCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
NPKRGDKVIAWARNFLDESAPLETGSWSDVTGFNIADGLLQLAIGAATTGLKDAVQFKGF
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCC
SGEAAKPATILLGKNGLHTEIVIDPSTEIGKSDRAGISDVILESALTTIMDCEDSVAAVD
CCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEC
AEDKVLVYGNWLGLMRGDLTEAVSKGGNTFTRRLNPDRYYTAPDGSALTLPGRSLMLVRN
CCCCEEEEECHHHHHHHHHHHHHHCCCCEEEECCCCCCEEECCCCCEEECCCCCEEEHHH
VGHLMTNPAILDRDGRDVPEGIMDAVVTALIALYDVGPSGRRQNSRAGSMYVVKPKMHGP
HHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCC
EEVAFANEIFARVENLVGMAPNTMKMGIMDEERRTTVNLKESIRAAKDRVVFINTGFLDR
HHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHEECHHHHHHHHHCCEEEEECCCCCC
TGDEIHTSMEAGPMIRKGDMKQAAWIAAYENWNVDIGLECGLSGHAQIGKGMWAMPDLMA
CCHHHHHHHHCCCCEECCCCCCEEEEEEECCCCEEEEEECCCCCCHHHCCCCCHHHHHHH
AMLEQKIAHPKAGANTAWVPSPTAATLHATHYHKVDVAAVQEGLKSRGRAKLSDILSVPV
HHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHCCC
APRPNWTPEEIQRELDNNAQGILGYVVRWVDQGVGCSKVPDINNIGLMEDRATLRISAQH
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEHHH
MANWLRHGVVTEAQIIKTMKRMAAVVDTQNAGDPAYLPMASDFDGSVAFQAAVELVLKGR
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHCC
EQPNGYTEPVLHRRRLELKAKQAG
CCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]