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The map label for this gene is folC [H]

Identifier: 15887380

GI number: 15887380

Start: 18250

End: 19593

Strand: Direct

Name: folC [H]

Synonym: Atu0021

Alternate gene names: 15887380

Gene position: 18250-19593 (Clockwise)

Preceding gene: 15887379

Following gene: 159184132

Centisome position: 0.64

GC content: 58.04

Gene sequence:

>1344_bases
ATGACGAAGCCCGCGATCGGTGAGGCCGAAAAGATCATTGAAGAGCTGATGCAGCTTCACCCCAAGGGGTTTGATCTTTC
ACTCGACAGAATTTCCCGTCTTCTCGAAAAACTCGGCAATCCGCAAAACAGAATGCCGCCGGTGATCCATGTGGCGGGCA
CCAATGGCAAGGGTTCCGTTAGCGCCTTTTGCCGGGCTTTGCTGGAAGCGGCCGGACTTCCTGTTCATGTCCATACCAAG
CCCCATCTCGTCAACTGGCATGAGCGCTATCGTCTCGGCGTTGCCGGGGAGAGGGGCCGCTTTGTTGAAGACGCTGTCTT
CGCGGACGCCCTGCGTCGCATCGAAGTCGCGAACGATGGGCAGAAGATCACCGTTTTTGAAATTTTGACTGCTGTCATGT
TCCTGCTTTTTTCGGAGCATCCCGCTGATGCTGCGATCGTCGAGGTTGGACTGGGCGGTCGCTTCGATGCAACCAATGTG
ATCAATAGCCCTGCCGTTTCCATCATCATGCCGATTTCGCTCGACCATGAAGCTTATCTCGGCGACCGCGTGGAACTGAT
TGCCGCCGAAAAGGCTGGCATCATGAAAAAGGGTTCGCCCGTCGTCATCGGCCATCAGGAATATGATGCGGCGCGTGAAA
CGCTCGTCGCTACGGCCGAGCGGCTTGGTTGCCTGGTTTCTGTCTACGGACAGGATTTTTCCGCCCATGAAGAATTCGGC
CGCATGGTCTACCAGGATGAATTCGGCTTGGCCGATGCGCCGTTGCCGCGTCTTCCCGGCCGCCATCAGCTCGCTAACGC
TGCCGCAGCGATCCGCGCCGTGAAAGCAGCCGGTTTCGAAGTGACGGAGCGGATGATCGAAAAGGCCATGACTTCAGTGG
AATGGCCCGGCCGTCTTCAGCGCATCCTGACCGGCAAAATCGCTGAAATCGCCCCGAAGGGTGCCGAGATCTGGATCGAT
GGCGGTCACAACCCCGGCGCGGGTGAGGTGATTGCCGAGGCCATGGCAGGTTTTGAGGAAAAGACGCCGCGCCCACTCTT
CATCATCGCTGGCATGATCAATACCAAGGACCCGGTGGGTTATTTCAAGGCTTTCGCCGATATTGCCGAATATGTCTTCA
CCGTGCCGATAGGCGGCACGGATGCGGCGATCGACCCTGTCGTGCTTGCCCATGCCGCTTTTGATGCCGGTCTGGTGGCC
GCGCCCACATCGACGCTGACCCATGCTCTGGAAGAATTGTCGCGCCGTGTAGATCCCGAAGGACCGCCGCCGCGCATCCT
TATCGGCGGATCGCTTTATCTTGCCGGAAACGCCCTTGCTTTCAACGGTACCGTTCCCCAATAA

Upstream 100 bases:

>100_bases
GGCATAAACTGCTTTCGGGATGATGCTATACGTTGCCTGATTTCATTTTTGGGGCAGCGGACAAATGGCAAACAAAACGC
CTGAAAGGCGAACGGAAGAC

Downstream 100 bases:

>100_bases
AAAAGCCCGGCGATCATGCCGGGCTTTTTTCATTCCATAAAAACGGCTTAAGCAGCGCTGGAAATCCAGGCGGAAAGCGC
TGTCTTGGGTGCAGCACCTA

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MTKPAIGEAEKIIEELMQLHPKGFDLSLDRISRLLEKLGNPQNRMPPVIHVAGTNGKGSVSAFCRALLEAAGLPVHVHTK
PHLVNWHERYRLGVAGERGRFVEDAVFADALRRIEVANDGQKITVFEILTAVMFLLFSEHPADAAIVEVGLGGRFDATNV
INSPAVSIIMPISLDHEAYLGDRVELIAAEKAGIMKKGSPVVIGHQEYDAARETLVATAERLGCLVSVYGQDFSAHEEFG
RMVYQDEFGLADAPLPRLPGRHQLANAAAAIRAVKAAGFEVTERMIEKAMTSVEWPGRLQRILTGKIAEIAPKGAEIWID
GGHNPGAGEVIAEAMAGFEEKTPRPLFIIAGMINTKDPVGYFKAFADIAEYVFTVPIGGTDAAIDPVVLAHAAFDAGLVA
APTSTLTHALEELSRRVDPEGPPPRILIGGSLYLAGNALAFNGTVPQ

Sequences:

>Translated_447_residues
MTKPAIGEAEKIIEELMQLHPKGFDLSLDRISRLLEKLGNPQNRMPPVIHVAGTNGKGSVSAFCRALLEAAGLPVHVHTK
PHLVNWHERYRLGVAGERGRFVEDAVFADALRRIEVANDGQKITVFEILTAVMFLLFSEHPADAAIVEVGLGGRFDATNV
INSPAVSIIMPISLDHEAYLGDRVELIAAEKAGIMKKGSPVVIGHQEYDAARETLVATAERLGCLVSVYGQDFSAHEEFG
RMVYQDEFGLADAPLPRLPGRHQLANAAAAIRAVKAAGFEVTERMIEKAMTSVEWPGRLQRILTGKIAEIAPKGAEIWID
GGHNPGAGEVIAEAMAGFEEKTPRPLFIIAGMINTKDPVGYFKAFADIAEYVFTVPIGGTDAAIDPVVLAHAAFDAGLVA
APTSTLTHALEELSRRVDPEGPPPRILIGGSLYLAGNALAFNGTVPQ
>Mature_446_residues
TKPAIGEAEKIIEELMQLHPKGFDLSLDRISRLLEKLGNPQNRMPPVIHVAGTNGKGSVSAFCRALLEAAGLPVHVHTKP
HLVNWHERYRLGVAGERGRFVEDAVFADALRRIEVANDGQKITVFEILTAVMFLLFSEHPADAAIVEVGLGGRFDATNVI
NSPAVSIIMPISLDHEAYLGDRVELIAAEKAGIMKKGSPVVIGHQEYDAARETLVATAERLGCLVSVYGQDFSAHEEFGR
MVYQDEFGLADAPLPRLPGRHQLANAAAAIRAVKAAGFEVTERMIEKAMTSVEWPGRLQRILTGKIAEIAPKGAEIWIDG
GHNPGAGEVIAEAMAGFEEKTPRPLFIIAGMINTKDPVGYFKAFADIAEYVFTVPIGGTDAAIDPVVLAHAAFDAGLVAA
PTSTLTHALEELSRRVDPEGPPPRILIGGSLYLAGNALAFNGTVPQ

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=357, Percent_Identity=28.2913165266106, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI66932984, Length=357, Percent_Identity=28.2913165266106, Blast_Score=118, Evalue=9e-27,
Organism=Escherichia coli, GI1788654, Length=377, Percent_Identity=32.8912466843501, Blast_Score=159, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17553150, Length=311, Percent_Identity=29.2604501607717, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17553148, Length=311, Percent_Identity=29.2604501607717, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71984923, Length=311, Percent_Identity=29.2604501607717, Blast_Score=118, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6323760, Length=442, Percent_Identity=28.7330316742081, Blast_Score=156, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6324815, Length=297, Percent_Identity=27.6094276094276, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6322718, Length=296, Percent_Identity=25.3378378378378, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24641571, Length=334, Percent_Identity=26.3473053892216, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24581568, Length=206, Percent_Identity=28.6407766990291, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47987; Mature: 47856

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPAIGEAEKIIEELMQLHPKGFDLSLDRISRLLEKLGNPQNRMPPVIHVAGTNGKGSV
CCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCH
SAFCRALLEAAGLPVHVHTKPHLVNWHERYRLGVAGERGRFVEDAVFADALRRIEVANDG
HHHHHHHHHHCCCCEEECCCCCEECHHHHEEECCCCCCCCHHHHHHHHHHHHHHHCCCCC
QKITVFEILTAVMFLLFSEHPADAAIVEVGLGGRFDATNVINSPAVSIIMPISLDHEAYL
CEEHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCHHHC
GDRVELIAAEKAGIMKKGSPVVIGHQEYDAARETLVATAERLGCLVSVYGQDFSAHEEFG
CCCEEEEEEHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHC
RMVYQDEFGLADAPLPRLPGRHQLANAAAAIRAVKAAGFEVTERMIEKAMTSVEWPGRLQ
HHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHH
RILTGKIAEIAPKGAEIWIDGGHNPGAGEVIAEAMAGFEEKTPRPLFIIAGMINTKDPVG
HHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCHHHCCCCCEEEEEECCCCCCHHH
YFKAFADIAEYVFTVPIGGTDAAIDPVVLAHAAFDAGLVAAPTSTLTHALEELSRRVDPE
HHHHHHHHHHHHEEECCCCCCCHHCHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHCCCC
GPPPRILIGGSLYLAGNALAFNGTVPQ
CCCCEEEECCEEEEECCEEEECCCCCC
>Mature Secondary Structure 
TKPAIGEAEKIIEELMQLHPKGFDLSLDRISRLLEKLGNPQNRMPPVIHVAGTNGKGSV
CCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCH
SAFCRALLEAAGLPVHVHTKPHLVNWHERYRLGVAGERGRFVEDAVFADALRRIEVANDG
HHHHHHHHHHCCCCEEECCCCCEECHHHHEEECCCCCCCCHHHHHHHHHHHHHHHCCCCC
QKITVFEILTAVMFLLFSEHPADAAIVEVGLGGRFDATNVINSPAVSIIMPISLDHEAYL
CEEHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCHHHC
GDRVELIAAEKAGIMKKGSPVVIGHQEYDAARETLVATAERLGCLVSVYGQDFSAHEEFG
CCCEEEEEEHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHC
RMVYQDEFGLADAPLPRLPGRHQLANAAAAIRAVKAAGFEVTERMIEKAMTSVEWPGRLQ
HHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHH
RILTGKIAEIAPKGAEIWIDGGHNPGAGEVIAEAMAGFEEKTPRPLFIIAGMINTKDPVG
HHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCHHHCCCCCEEEEEECCCCCCHHH
YFKAFADIAEYVFTVPIGGTDAAIDPVVLAHAAFDAGLVAAPTSTLTHALEELSRRVDPE
HHHHHHHHHHHHEEECCCCCCCHHCHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHCCCC
GPPPRILIGGSLYLAGNALAFNGTVPQ
CCCCEEEECCEEEEECCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]