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The map label for this gene is buk1 [H]

Identifier: 239618524

GI number: 239618524

Start: 2295648

End: 2296718

Strand: Reverse

Name: buk1 [H]

Synonym: Kole_2165

Alternate gene names: 239618524

Gene position: 2296718-2295648 (Counterclockwise)

Preceding gene: 239618525

Following gene: 239618523

Centisome position: 99.77

GC content: 45.1

Gene sequence:

>1071_bases
ATGCCCAGAATATTGATTATAAACCCGGGTTCCACTTCAACGAAACTTGGTATCTATGAAAACGATGAGATCATCTGGAA
AGAGAATTTTGAACATTCCGCTCACACTGAAGATTTATTGACACCCGAGGAAAGGGTGAAAATTCGAGCAAAGGAAATCG
AAGGTCTTCTGTCAAAAGAGGGGTTCAAAATAGAAGAATTTGATGCTATAGCCTGTAGAGGCGGCATACTCCCGCCGCTA
GAAAGTGGGACCTATGAGGTAGATGAAGAAATGGTTGACTATTTGCTTCACAAAACAGGTGTTGACCACCCTTCAAACCT
GGCAGCGCCAATTGGAAGATTGCTTTCCAGAGGAAATATCCCCGTCTACATAACCGATCCGGTTTCAGTAGACGAGTTTT
GGGATGAAGCAAGGCTTTCAGGGATTCCGCAAATAGAGCGCCGGAGCCTCTTTCATGCCCTTAATATGAAAGCGGTTGCA
AGGAAGGTAGCAGAAGAAATGGGAAAGCCTCTGGAAGAAATGAATTTTGTGATAGCTCATCTCGGCGGCGGGATCTCCGT
AGGGCTTCAGGTTAAAGGAAAAATGGTGGATGTAAATAATGCAAACGATGAAGGCCCCTTTAGTCCTAACAGGACTGGAG
AGCTTCCTGTTGGCGATGTAGTAGAGCTGGCTTTTAGTGGAAAATATACCGAAAAGGAGCTTCTGAAAAGATACACCAAG
CAGGGAGGGCTTCTGGCTTATCTGGGAACAGACGACTTGAGAAAAGCGATGAAACTTGCTGCAAATGATCCTAAGGCAGA
GCTTGTAATTGAAGGAATGGCTTATCAAATTGCCAAAGAGATCGGTGGAATGTGTGCGGTAGCCGGTGGAAATATTGACG
CGATAATTCTTACGGGTAGTATGGCTTATAACTCGGACTTCGTGGAGAAAATCAAGAGCTACGTTGCCCGTTTTGCGTTG
GTGGTCGTTGAGCCTGGAGAAAATGAACTCCTTGCGTTAGCTGAAGGTGCTAAACGTGTTTTAACAGGGAAAGAGAACCC
AAAGCGTTTCAAGGTGGGTGAAACTATATGA

Upstream 100 bases:

>100_bases
GTTTGCCTCCTGAGAAGGAAATCATAGAAGGAGTTGCCAGGCTTAAGAAAACTGTTCTCGAATACGGAAAAATGAAAGGT
TTACTGTAAGAGGTGATTTT

Downstream 100 bases:

>100_bases
GTACAATAAGGACTCTTTCAGAGATAGTTGAACGTGTTCGTGGAAGAAAGGGGATTGTGGCTGTTGCTGTAGCTGAAGAT
GTCGTTGTTTTAAAAGCGAT

Product: butyrate kinase

Products: NA

Alternate protein names: BK 1; Branched-chain carboxylic acid kinase 1 [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MPRILIINPGSTSTKLGIYENDEIIWKENFEHSAHTEDLLTPEERVKIRAKEIEGLLSKEGFKIEEFDAIACRGGILPPL
ESGTYEVDEEMVDYLLHKTGVDHPSNLAAPIGRLLSRGNIPVYITDPVSVDEFWDEARLSGIPQIERRSLFHALNMKAVA
RKVAEEMGKPLEEMNFVIAHLGGGISVGLQVKGKMVDVNNANDEGPFSPNRTGELPVGDVVELAFSGKYTEKELLKRYTK
QGGLLAYLGTDDLRKAMKLAANDPKAELVIEGMAYQIAKEIGGMCAVAGGNIDAIILTGSMAYNSDFVEKIKSYVARFAL
VVVEPGENELLALAEGAKRVLTGKENPKRFKVGETI

Sequences:

>Translated_356_residues
MPRILIINPGSTSTKLGIYENDEIIWKENFEHSAHTEDLLTPEERVKIRAKEIEGLLSKEGFKIEEFDAIACRGGILPPL
ESGTYEVDEEMVDYLLHKTGVDHPSNLAAPIGRLLSRGNIPVYITDPVSVDEFWDEARLSGIPQIERRSLFHALNMKAVA
RKVAEEMGKPLEEMNFVIAHLGGGISVGLQVKGKMVDVNNANDEGPFSPNRTGELPVGDVVELAFSGKYTEKELLKRYTK
QGGLLAYLGTDDLRKAMKLAANDPKAELVIEGMAYQIAKEIGGMCAVAGGNIDAIILTGSMAYNSDFVEKIKSYVARFAL
VVVEPGENELLALAEGAKRVLTGKENPKRFKVGETI
>Mature_355_residues
PRILIINPGSTSTKLGIYENDEIIWKENFEHSAHTEDLLTPEERVKIRAKEIEGLLSKEGFKIEEFDAIACRGGILPPLE
SGTYEVDEEMVDYLLHKTGVDHPSNLAAPIGRLLSRGNIPVYITDPVSVDEFWDEARLSGIPQIERRSLFHALNMKAVAR
KVAEEMGKPLEEMNFVIAHLGGGISVGLQVKGKMVDVNNANDEGPFSPNRTGELPVGDVVELAFSGKYTEKELLKRYTKQ
GGLLAYLGTDDLRKAMKLAANDPKAELVIEGMAYQIAKEIGGMCAVAGGNIDAIILTGSMAYNSDFVEKIKSYVARFALV
VVEPGENELLALAEGAKRVLTGKENPKRFKVGETI

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG3426

COG function: function code C; Butyrate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=168, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI145693184, Length=335, Percent_Identity=24.1791044776119, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR011245 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.7 [H]

Molecular weight: Translated: 39027; Mature: 38896

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS01075 ACETATE_KINASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRILIINPGSTSTKLGIYENDEIIWKENFEHSAHTEDLLTPEERVKIRAKEIEGLLSKE
CCEEEEECCCCCCEEEEEEECCEEEEECCCCCCCCCHHCCCCHHHHEEHHHHHHHHHHCC
GFKIEEFDAIACRGGILPPLESGTYEVDEEMVDYLLHKTGVDHPSNLAAPIGRLLSRGNI
CCCCHHCCHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCC
PVYITDPVSVDEFWDEARLSGIPQIERRSLFHALNMKAVARKVAEEMGKPLEEMNFVIAH
CEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEEE
LGGGISVGLQVKGKMVDVNNANDEGPFSPNRTGELPVGDVVELAFSGKYTEKELLKRYTK
CCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
QGGLLAYLGTDDLRKAMKLAANDPKAELVIEGMAYQIAKEIGGMCAVAGGNIDAIILTGS
CCCEEEEECCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCEEEEECCCEEEEEEECC
MAYNSDFVEKIKSYVARFALVVVEPGENELLALAEGAKRVLTGKENPKRFKVGETI
CCCCHHHHHHHHHHHHHEEEEEECCCCCCEEEEHHCCHHHHCCCCCCCEEECCCCC
>Mature Secondary Structure 
PRILIINPGSTSTKLGIYENDEIIWKENFEHSAHTEDLLTPEERVKIRAKEIEGLLSKE
CEEEEECCCCCCEEEEEEECCEEEEECCCCCCCCCHHCCCCHHHHEEHHHHHHHHHHCC
GFKIEEFDAIACRGGILPPLESGTYEVDEEMVDYLLHKTGVDHPSNLAAPIGRLLSRGNI
CCCCHHCCHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCC
PVYITDPVSVDEFWDEARLSGIPQIERRSLFHALNMKAVARKVAEEMGKPLEEMNFVIAH
CEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEEE
LGGGISVGLQVKGKMVDVNNANDEGPFSPNRTGELPVGDVVELAFSGKYTEKELLKRYTK
CCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
QGGLLAYLGTDDLRKAMKLAANDPKAELVIEGMAYQIAKEIGGMCAVAGGNIDAIILTGS
CCCEEEEECCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCEEEEECCCEEEEEEECC
MAYNSDFVEKIKSYVARFALVVVEPGENELLALAEGAKRVLTGKENPKRFKVGETI
CCCCHHHHHHHHHHHHHEEEEEECCCCCCEEEEHHCCHHHHCCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]