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The map label for this gene is thiP [H]

Identifier: 239618512

GI number: 239618512

Start: 2282481

End: 2284085

Strand: Reverse

Name: thiP [H]

Synonym: Kole_2153

Alternate gene names: 239618512

Gene position: 2284085-2282481 (Counterclockwise)

Preceding gene: 239618513

Following gene: 239618509

Centisome position: 99.22

GC content: 42.49

Gene sequence:

>1605_bases
GTGTTATCTACGAATAAATTCTATCTGGCTCTTTTTTTGACGTTAGCAATCGCGGTTCTCTTTATACTTTATCCATTGTT
CGCTTCCTTTGGCTCCGCCAGAGGAAGCGAAGTTTTGACAATAATAAGAAGCAATACAATTCAGAAGATTTTGAAATTTA
CTGTGTTTCAGGCAACAATAAGCACGCTGCTGGCTGTTCTTGTTGGATTTCCGGCTGCTTATTACTATGCCCACCACAAT
GATACTCTAGCGAAACTCCTTTACATAACGACGTTCATTCCGTTTTTTTTACCGGGGATATCAGCGGTTGTAGGTTTTCT
GATAATGTACGGAAGAAATGGTATACTCAATTCCTTCCTGGGGATTTTTGGGATCAAGCTTCAGATTCTTTACACCTTCA
CCGCGATCTTGCTTGGACATATTTTTTACAACGCCCCGATACTTGTTAGAGTTCTCGGTGATGCACTTGCAGGAATCCCA
AAAGAACAGATAGAAAACGCACTGTCTGATGGTGCGAATAAATGGAAGATATTCTGGAAGATTGAATTTCCTCTGGTTCT
TCCTGCATTAGTGGCCGGAACTATTCTCATTTTTTCTTACTGCTTCACTTCCTTTGCTGTTGTTTTGATTCTCGGTGGCT
CGCAGTACGCGACCCTGGAGGTTGCGATTTACATGTACCTCAAACTACTTGCCAAACCAAATATCGCGATTTCTCTGGCA
ATACTCCAATTTCTTTTCATATTTGCCTTTGGATTTTTTCTTTCTTTCTTCGAAAAGAGAGAGAGCTTTCAATATGGAGA
ACGGTACATTGAAAAAAGAAAATACCTGAAGGTTTACTCCGTTCTCTTTCTGATTTTTGAAAGCGCGCCGGTTATAGCGG
GTTTTGCTGGATCGGTTTACGATTTTCGAAGGAAAGAATTTCTCTTTGACAGGATAGTGACCATCTTTACATCATCGCAG
ATTCCTATCGTGGGTTCTACCATCCCGGAGGCAATTTTAAGATCGCTGCTATTGTCAGCAACCACAGCCTTAATAGGAAT
GGTTCTCGGTTTTTCTATGATCTGGGGATTAAGAGATACACCTCGTGGAAGCTTTATTTTAAGAGGTATTTCTCTTCTAA
GTATATCAATAACCCCGGCTATACTTGCTCTATCTTACAGCACTTCTTTTAAGTTTATTGCACCACCGCTGGCCATGATT
CTGCTCTACCTTGTGGTATCCCTTCCAATCAATCTGAACCTGATCCACGCTGGAACCGCTGGTGTGAGCGTAACGGCAAT
AGAAGCGGCAAAAATAGATGGTGCCAGCTCATTGCAAATTCTGTGGCATATTATCGTTCCTCTTTTCAGAAAGGTGCTTA
TCGCATCCTTCAGTGTGATCTTTGCGATATCTATGGGCGAATTCAGCGGGTCTCTAATCCTCGGTGGAGATAGCTATCCC
ACAATAACCGTTGCCATATATCGGTTGATGTCCAGCCGCCATCTCATCGAAGCGAGATTTCTGAGCTCTTTGCTGGTGAT
TGTCGTTCTGGTCTCACTGGCACTGATAGGCGGTTTCTTCACCTCTTTTTCTGAACATCTGAGAAATACCGGCAGCAGGA
ATTGA

Upstream 100 bases:

>100_bases
ATTGATCTGCCAGAAGCGTTTAACTACGCGGTGAAACCTACCGAACTTCTCTCTGTTCCTGAAAATATCGATATTGAAAA
AATTATTGCCCTCTGGGAGA

Downstream 100 bases:

>100_bases
TGGGACACTCATTGCATTGTGGATTTACAGGCTTGCACACCCTTCGGCCGAATTCCACCATAGAACCATTCAAAGGTCCC
CACAATTCCTCCGGTAAAAT

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MLSTNKFYLALFLTLAIAVLFILYPLFASFGSARGSEVLTIIRSNTIQKILKFTVFQATISTLLAVLVGFPAAYYYAHHN
DTLAKLLYITTFIPFFLPGISAVVGFLIMYGRNGILNSFLGIFGIKLQILYTFTAILLGHIFYNAPILVRVLGDALAGIP
KEQIENALSDGANKWKIFWKIEFPLVLPALVAGTILIFSYCFTSFAVVLILGGSQYATLEVAIYMYLKLLAKPNIAISLA
ILQFLFIFAFGFFLSFFEKRESFQYGERYIEKRKYLKVYSVLFLIFESAPVIAGFAGSVYDFRRKEFLFDRIVTIFTSSQ
IPIVGSTIPEAILRSLLLSATTALIGMVLGFSMIWGLRDTPRGSFILRGISLLSISITPAILALSYSTSFKFIAPPLAMI
LLYLVVSLPINLNLIHAGTAGVSVTAIEAAKIDGASSLQILWHIIVPLFRKVLIASFSVIFAISMGEFSGSLILGGDSYP
TITVAIYRLMSSRHLIEARFLSSLLVIVVLVSLALIGGFFTSFSEHLRNTGSRN

Sequences:

>Translated_534_residues
MLSTNKFYLALFLTLAIAVLFILYPLFASFGSARGSEVLTIIRSNTIQKILKFTVFQATISTLLAVLVGFPAAYYYAHHN
DTLAKLLYITTFIPFFLPGISAVVGFLIMYGRNGILNSFLGIFGIKLQILYTFTAILLGHIFYNAPILVRVLGDALAGIP
KEQIENALSDGANKWKIFWKIEFPLVLPALVAGTILIFSYCFTSFAVVLILGGSQYATLEVAIYMYLKLLAKPNIAISLA
ILQFLFIFAFGFFLSFFEKRESFQYGERYIEKRKYLKVYSVLFLIFESAPVIAGFAGSVYDFRRKEFLFDRIVTIFTSSQ
IPIVGSTIPEAILRSLLLSATTALIGMVLGFSMIWGLRDTPRGSFILRGISLLSISITPAILALSYSTSFKFIAPPLAMI
LLYLVVSLPINLNLIHAGTAGVSVTAIEAAKIDGASSLQILWHIIVPLFRKVLIASFSVIFAISMGEFSGSLILGGDSYP
TITVAIYRLMSSRHLIEARFLSSLLVIVVLVSLALIGGFFTSFSEHLRNTGSRN
>Mature_534_residues
MLSTNKFYLALFLTLAIAVLFILYPLFASFGSARGSEVLTIIRSNTIQKILKFTVFQATISTLLAVLVGFPAAYYYAHHN
DTLAKLLYITTFIPFFLPGISAVVGFLIMYGRNGILNSFLGIFGIKLQILYTFTAILLGHIFYNAPILVRVLGDALAGIP
KEQIENALSDGANKWKIFWKIEFPLVLPALVAGTILIFSYCFTSFAVVLILGGSQYATLEVAIYMYLKLLAKPNIAISLA
ILQFLFIFAFGFFLSFFEKRESFQYGERYIEKRKYLKVYSVLFLIFESAPVIAGFAGSVYDFRRKEFLFDRIVTIFTSSQ
IPIVGSTIPEAILRSLLLSATTALIGMVLGFSMIWGLRDTPRGSFILRGISLLSISITPAILALSYSTSFKFIAPPLAMI
LLYLVVSLPINLNLIHAGTAGVSVTAIEAAKIDGASSLQILWHIIVPLFRKVLIASFSVIFAISMGEFSGSLILGGDSYP
TITVAIYRLMSSRHLIEARFLSSLLVIVVLVSLALIGGFFTSFSEHLRNTGSRN

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1178

COG function: function code P; ABC-type Fe3+ transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

Organism=Escherichia coli, GI1786254, Length=219, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1787369, Length=170, Percent_Identity=27.0588235294118, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787080, Length=214, Percent_Identity=26.1682242990654, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1788764, Length=195, Percent_Identity=26.1538461538462, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005947 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 58936; Mature: 58936

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSTNKFYLALFLTLAIAVLFILYPLFASFGSARGSEVLTIIRSNTIQKILKFTVFQATI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
STLLAVLVGFPAAYYYAHHNDTLAKLLYITTFIPFFLPGISAVVGFLIMYGRNGILNSFL
HHHHHHHHHCCHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
GIFGIKLQILYTFTAILLGHIFYNAPILVRVLGDALAGIPKEQIENALSDGANKWKIFWK
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEE
IEFPLVLPALVAGTILIFSYCFTSFAVVLILGGSQYATLEVAIYMYLKLLAKPNIAISLA
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
ILQFLFIFAFGFFLSFFEKRESFQYGERYIEKRKYLKVYSVLFLIFESAPVIAGFAGSVY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHH
DFRRKEFLFDRIVTIFTSSQIPIVGSTIPEAILRSLLLSATTALIGMVLGFSMIWGLRDT
HHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PRGSFILRGISLLSISITPAILALSYSTSFKFIAPPLAMILLYLVVSLPINLNLIHAGTA
CCCHHHHHHHHHHEEEHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCC
GVSVTAIEAAKIDGASSLQILWHIIVPLFRKVLIASFSVIFAISMGEFSGSLILGGDSYP
CCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEECCCCCC
TITVAIYRLMSSRHLIEARFLSSLLVIVVLVSLALIGGFFTSFSEHLRNTGSRN
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLSTNKFYLALFLTLAIAVLFILYPLFASFGSARGSEVLTIIRSNTIQKILKFTVFQATI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
STLLAVLVGFPAAYYYAHHNDTLAKLLYITTFIPFFLPGISAVVGFLIMYGRNGILNSFL
HHHHHHHHHCCHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
GIFGIKLQILYTFTAILLGHIFYNAPILVRVLGDALAGIPKEQIENALSDGANKWKIFWK
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEE
IEFPLVLPALVAGTILIFSYCFTSFAVVLILGGSQYATLEVAIYMYLKLLAKPNIAISLA
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
ILQFLFIFAFGFFLSFFEKRESFQYGERYIEKRKYLKVYSVLFLIFESAPVIAGFAGSVY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHH
DFRRKEFLFDRIVTIFTSSQIPIVGSTIPEAILRSLLLSATTALIGMVLGFSMIWGLRDT
HHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PRGSFILRGISLLSISITPAILALSYSTSFKFIAPPLAMILLYLVVSLPINLNLIHAGTA
CCCHHHHHHHHHHEEEHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCC
GVSVTAIEAAKIDGASSLQILWHIIVPLFRKVLIASFSVIFAISMGEFSGSLILGGDSYP
CCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEECCCCCC
TITVAIYRLMSSRHLIEARFLSSLLVIVVLVSLALIGGFFTSFSEHLRNTGSRN
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA